scholarly journals The effect of guar gum on the viscosity of the gastrointestinal contents and on glucose uptake from the perfused jejunum in the rat

1981 ◽  
Vol 46 (2) ◽  
pp. 239-246 ◽  
Author(s):  
N. A. Blackburn ◽  
I. T. Johnson
Keyword(s):  
Small Intestine ◽  
Glucose Uptake ◽  
Large Intestine ◽  
Apparent Viscosity ◽  
Wistar Rats ◽  
Guar Gum ◽  
Glucose Absorption ◽  
Synthetic Diet ◽  
Male Wistar Rats ◽  
Small And Large Intestine

1. Male Wistar rats were meal-fed for at least 10d a control semi-synthetic diet containing no guar gum, or one of three similar test diets containing 3, 10 or 20 g dry guar gum/kg.2. Rats were killed 6 h after feeding, and contents of stomach, small and large intestine were collected separately. The apparent viscosities of stomach and smalt intestine contents from animals fed on diets containing 10 and 20 g guar gum/kg were increased relative to control animals, but large intestine contents were unchanged3. In the second part of this study, male Wistar rats were anaesthetized and two consecutive lengths of jejunum were perfused, initially with Ringer only (control) or Ringer plus 5 or 6g guar gum/1 (test). Following this pre-perfusion, both segments were perfused with Ringer containing glucose (10 mM), [3H]glucose and [14C]inulin, and the rate of glucose absorption was determined4. The rate of glucose absorption was decreased relative to control values in segments pre-perfused with both 5 and 6g guar gum/I solution, but this reduction was significant only in the instance of the 6g/l solution (P< 0.001)5. These results provide evidence to support previous assumptions that ingestion of guar gum will increase the apparent viscosity of the contents of the stomach and small intestine. We propose that a possible mechanism by which guar reduces post-prandial glycaemia is a reduction of glucose absorption from the smali intestine, resulting from an increase in viscosity of the contents

scholarly journals The effect of heat treatment and particle size of bran on mineral absorption in rats

1982 ◽  
Vol 48 (3) ◽  
pp. 467-475 ◽  
Author(s):  
Andrea Caprez ◽  
Susan J. Fairweather-Tait
Keyword(s):  
Heat Treatment ◽  
Particle Size ◽  
Isotope Dilution ◽  
Wistar Rats ◽  
Specific Radioactivity ◽  
Young Male ◽  
Breakfast Cereal ◽  
Synthetic Diet ◽  
Male Wistar Rats ◽  
The Mean

1. The effect of heat treatment of bran on true zinc absorption was measured using an isotope-dilution technique. A bran-based breakfast cereal (heated to 204° for 40 min during manufacture) was incorporated into a semi-synthetic diet at a level of 180 g/kg, A parallel diet was formulated containing an identical weight of untreated bran from the same source plus other ingredients used to make the cereal.2. Young male Wistar rats (mean weight 80 g) were injected intramuscularly with 65Zn to label body Zn. They were given the heat-treated- and untreated-bran diets for 9 d. During the last 6 d of this period Zn intakes and faecal and urinary Zn were measured in order to calculate apparent Zn retention. True Zn retention was measured by taking into account losses of Zn of endogenous origin (labelled with 65Zn), by measuring faecal and urinary 65Zn taking the mean specific radioactivity of Zn in kidneys and upper small intestine to represent specific radioactivity of endogenous origin.3. Heat treatment of bran removed approximately one-third of the phytate, but this was not enough to improve Zn absorption from the diet. True Zn retention measured by isotope dilution was significantly higher (P < 0.02) than apparent Zn retention measured bv the conventional balance technique.4. The hypothesis that a reduction in particle size of bran would improve mineral availability was tested by feeding coarse and milled bran (100 g/kg diet) in a semi-synthetic diet to rats and measuring true Fe and apparent Zn absorptions. The importance of phytate was also investigated by feedino a diet containing dephytinized bran.5. Male Wistar rats (mean weight 172 g) were given diets containing coarse, milled or dephytinized bran for 9 d. Fe and Zn intakes were measured and faeces and urine collected for Fe and Zn analysis.6. The mean (±SE) particle size of the bran was reduced on milling from 3.5 (± 1.8) to 0.2–0.5 mm. There were no differences in the fraction of Fe retained between the three groups. Particle size had a small effect on Zn retention which was marginally higher in rats on the milled-bran diet (0.126 (± 0.023)) than in those on the coarse-bran diet (0.087 (± 0.012)). Total removal of phytate had a greater effect and apparent Zn retention from the dephytinized-bran diet was significantly higher (0.182 (±0.027), P < 0.01).

scholarly journals The effect of dietary protein source and guar gum on gastrointestinal growth and enteroglucagon secretion in the rat

1987 ◽  
Vol 58 (1) ◽  
pp. 65-72 ◽  
Author(s):  
Susan Southon ◽  
Jennifer M. Gee ◽  
I. T. Johnson
Keyword(s):  
Dietary Protein ◽  
Wistar Rats ◽  
Guar Gum ◽  
Protein Source ◽  
Endocrine Activity ◽  
Intestinal Length ◽  
Daily Food ◽  
Male Wistar Rats ◽  
Small Intestinal ◽  
Dietary Protein Source

1. Male Wistar rats (approximately 100 g) were given fibre-free semi-synthetic diets containing either casein or albumin (168 g/kg diet) as the protein source with or without guar gum (75 g/kg diet) (casein, albumin, casein- guar gum and albumin-guar gum groups).2. Small intestinal length, weights of caecal tissue and contents and plasma enteroglucagon concentration were significantly increased in guar-gum-fed animals compared with the fibre-free groups.3. Total caecal weight and plasma enteroglucagon concentration were higher in the albumin-guar gum group compared with the casein-guar gum group. The weights of caecal tissue and contents were significantly increased in rats given the fibre-free albumin diet compared with those consuming a similar diet with casein as the protein source, although daily food intake tended to be lower.4. It is concluded that the effect of materials classed as dietary fibre may be significantly influenced by the non- polysaccharide component of the diet, and that such interactions may influence both the growth and endocrine activity of the gastrointestinal tract.

scholarly journals Experimental Type 2 Diabetes Induces Enzymatic Changes in Isolated Rat Enterocytes

2003 ◽  
Vol 4 (2) ◽  
pp. 119-123 ◽  
Author(s):  
Isabel M. Martínez ◽  
Inmaculada Morales ◽  
Guadalupe García-Pino ◽  
José E. Campillo ◽  
María A. Tormo
Keyword(s):  
Type 2 Diabetes ◽  
Small Intestine ◽  
Glucose Absorption ◽  
Diabetic Rats ◽  
Male Wistar Rats ◽  
Experimental Type ◽  
Enzymatic Changes ◽  
Rat Enterocytes ◽  
Intestinal Disaccharidases

Diabetes in humans and in experimental animals produces changes in the function and structure of the small intestine. The authors determined the activity of intestinal disaccharidases (maltase and sucrase) and of 6-phosphofructo-1-kinase (PFK-1) in enterocytes isolated from the small intestine of male Wistar rats (2.5 to 3 months old) with experimental nonobese type 2 diabetes, induced by streptozotocin (STZ) injection on the day of birth (n0-STZ) or on the 5th day of life (n5-STZ), with different degrees of hyperglycemia and insulinemia (n0-STZ and n5-STZ models). The glycemia (mmol/L) of the diabetic rats (n0-STZ: 8.77 ± 0.47; n5-STZ: 20.83 ± 0.63) was higher (P< .01) than that of the nondiabetic (ND) rats (5.99 ± 0.63); on the contrary, the insulinemia (ng/mL) was significantly lower in both n0-STZ (1.74 ± 0.53;P< .05) and n5-STZ (1.12 ± 0.44;P< .01) diabetic rats than in normal rats (3.77 ± 0.22). The sucrase and maltase activities (U/g protein) in diabetic rats (n0-STZ: 89 ± 9 and 266 ± 12; n5-STZ: 142 ± 23 and 451 ± 57) were significantly higher than those in the ND group (66 ± 5 and 228 ± 22). The PFK-1 activities (mU/mg protein) in the diabetic models (n0-STZ: 14.89 ± 1.51; n5-STZ: 13.35 ± 3.12) were significantly lower (P< .05) than in ND rats (20.54 ± 2.83). The data demonstrated enzymatic alterations in enterocytes isolated fromthe small intestine of n0-STZ rats that are greater (P< .05) than in the more hyperglycemic and hypoinsulinemic n5-STZ animals. The results also show that nonobese type 2–like diabetes in the rat produces modifications that favor an increase in glucose absorption rates.

scholarly journals The effects of dietary sugar-beet fibre and guar gum on lipid metabolism in Wistar rats

1994 ◽  
Vol 72 (3) ◽  
pp. 385-395 ◽  
Author(s):  
P. D. Overton ◽  
N. Furlonger ◽  
J. M Beety ◽  
J. Chakraborty ◽  
J. A. Tredger ◽  
...  
Keyword(s):  
Bile Acid ◽  
Sugar Beet ◽  
Wistar Rats ◽  
Guar Gum ◽  
Acid Output ◽  
Dietary Lipid ◽  
Hepatic Cholesterol ◽  
Male Wistar Rats ◽  
Rate Of Absorption ◽  
Coa Reductase

This study investigates the mechanisms of action for the hypocholesterolaemic effects of sugar-beet fibre (SBF) and gum gum. Four groups of ten male Wistar rats were fed ad lib. on test diets containing either 100 g SBF or guar/kg, or control diets containing 100 g cellulose or wheat bran/kg for 28 d. Food intake, weight gain and food consumption ratios were unaffected by the diets. Circulating cholesterol and hepatic cholesterol concentrations were significantly lower in both SBF- and guar-fed groups compared with either cellulose- or bran-fed animals. Circulating triacylglycerol concentrations were significantly lower in SBF- and guar-fed animals, but total hepatic lipid concentrations and hepatic and adipose tissue lipogenesis rates were unaffected by the diets. Hepatic cholesterol-7α-hydroxylase (EC1.14.13.17) activities were significantly higher in the guar-fed animals compared with cellulose or bran control groups. Hepatic 3-hydroxy-3-metbylglutaryl-CoA reductase (EC1.1.1.88) activities were unaffected. Circulating bile acid concentrations were significantly lower in SBF- and guar-fed animals and faecal bile acid output was significantly higher in the guar-fed group compared with bran- or cellulose-fed groups. This study supports the hypothesis that guar exerts its hypocholesterolaemic effect via intraluminal bile acid binding and loss of cholesterol from increased faecal bile acid excretion. The mechanism of action for the hypocholesterolaemic effect of SBF is less clear; the results of the present study point to a mechanism involving disruption of the enterohepatic bile acid circulation, possibly via changes in the rate of absorption of dietary lipid.

scholarly journals THE EFFECT OF VARYING LEVELS OF RAW LIMA BEAN (Phasealus lunatus) ON THE RAT'S PANCREATIC AND INTESTINAL TRYPSIN (EC 3.4.21.4) AND CHYMOTRYPSIN (EC 3.4.21.1) ACTIVITIES

2021 ◽  
Vol 11 (2) ◽  
pp. 140-147
Author(s):  
V. A. Aletor ◽  
B. L. Fetuga
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Lima Bean ◽  
Trypsin Activity ◽  
Growing Rats ◽  
Multiple Determination ◽  
Proteinase Inhibition ◽  
Small And Large Intestine ◽  
Pancreatic Proteinase

Two series of assays involving a total of 120 growing rats were carried out to investigate the effect of varying levels of raw lima bean (RLB) on pancreatic and intestinal trypsin EC 3.4.21.4) and chymotrypsin (EC 3.4.21.1) activities. Experiment one indicates significant (P<0.01) inhibtion of both pancreatic trypsin and chymotrypsin due to RLB feeding. Enzyme activities in both the small and large intestine were also significantly (P<0.01) depressed while enzyme values in the caecum were not. Age x Diet interaction was non significant with respect to these enzymes. Pancreatic trypsin and chymotrypsin correlated significantly (P<0.01; P<0.05) with RLB with respective R2, coefficient of multiple determination, of 0.94 and 0.67. Trypsin activity in both the small and large intestine was also significantly (P<0.01) correlated with respective R2 of 0.78 and 0.96. The second study suggests a less than 10% replacement of cooked lima bean by the raw to avert significant pancreatic proteinase inhibition, and a less than 15% replacement of the cooked lima bean by the raw to aver t significantinhibition of the proteinases especially in the small intestine.

scholarly journals Clinical and experimental substantiation of the effect of hypoxia on the wall of the small and large intestine in newborns

2018 ◽  
Vol 11 (4) ◽  
pp. 268-274
Author(s):  
Irina Yuryevna Karpova ◽  
Vyacheslav Vladimirovich Parshikov ◽  
Natalia Nikolaevna Prodanets ◽  
Tatyana Ivanovna Solovieva ◽  
Evgenia Dmitrievna Pyatova ◽  
...  
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Muscle Layer ◽  
Intestinal Wall ◽  
Case Histories ◽  
Tissue Component ◽  
White Rats ◽  
Connective Tissue Component ◽  
Small And Large Intestine ◽  
Experimental Substantiation

Based on the analysis of morphological and morphometric data, the clinical and experimental results of the effect of hypoxia on the wall of the small and large intestine in newborns are presented. The clinical part is based on the study of 58 case histories of patients with necrotizing enterocolitis, which the operating material (resected sections of the small intestine, large intestine) was studied in detail. The experimental work included the modeling of chronic hypobaric hypoxia in different trimesters of pregnancy in 4 groups of white rats (24 females). The offspring was taken out of the experiment at 4-5 days after birth, followed by collection of the small and large intestine areas to study histoarchitectonics. It is proved that hypoxia affects the number of newborn offspring, its weight. The small intestine is most vulnerable to the effect of this factor. Analysis of the morphological and morphometric picture of the operating and experimental material convincingly proves that under the influence of hypoxia the destruction of the intestinal mucosa occurs and the growth of the connective tissue component in the muscle layer occurs. Changes from the microcirculation of blood and lymph provoke the development of pre-ulcerative, ulcerative defects and the formation of necrosis. Disturbance of the drainage function promotes the spread of destruction in the intestinal wall.

scholarly journals 7. Effect of Soya Wastes Probiotic and Palm Kernel Addition Fermented by Aspergillus niger to the Cellulase Activity in Broilers Digestive Tract

2019 ◽  
Vol 13 (1) ◽  
pp. 47-54
Author(s):  
Nurliana Nurliana ◽  
Anna Farida ◽  
Sugito Sugito ◽  
Al Azhar ◽  
Razali Razali ◽  
...  
Keyword(s):  
Small Intestine ◽  
Digestive Tract ◽  
Large Intestine ◽  
Broiler Chickens ◽  
Palm Kernel ◽  
Cellulase Activity ◽  
Feed Supplement ◽  
Concentration Data ◽  
Two Factors ◽  
Small And Large Intestine

This study aimed to determine the cellulase activity in broilers digestive tract after the treatment of soya wastes and palm kernel fermented by A.niger (AKBIS Prob) in the ration. This study used a complete randomized factorial design, which consists of two factors, namely the AKBIS Prob composition factor and the duration of AKBIS Prob. Twenty four broilers were divided into four groups by randomized and three replications of each. The feed supplement combinations were P0 (commercial), P1 (commercial and AKBIS Prob 2%), P2 (commercial and AKBIS Prob 4%), and P3 (commercial and AKBIS Prob 6%). The intestine preparation was taken at the 22 and 36 days to extraction and detection the cellulase activity concentration. Data were analyzed using variance analysis of factorial patterns. AKBIS Prob 2,4 and 6% was no effect (P 0.05) to the cellulase activity in the small and large intestine, while the treatment duration was significant (P0,05) in the small intestine but neither in the large intestine (P0,05) to the cellulase activity. The addition of AKBIS Prob 2.4 and 6% had no effect (P 0.05) on the concentration of cellulase activity in the small intestine and large intestine, while the duration the treatment was very significant (P 0.05) in the small intestine and neither in the large intestine (P 0.05) on the concentration of cellulase activity. The AKBIS administration in the ration for 22 days can increase the activity and concentration of cellulase activity in the small intestine of the digestive tract of broiler chickens.

scholarly journals Neural motor complexes propagate continuously along the full length of mouse small intestine and colon

2020 ◽  
Vol 318 (1) ◽  
pp. G99-G108 ◽  
Author(s):  
Marcello Costa ◽  
Timothy James Hibberd ◽  
Lauren J. Keightley ◽  
Lukasz Wiklendt ◽  
John W. Arkwright ◽  
...  
Keyword(s):  
Small Intestine ◽  
Large Intestine ◽  
Motor Behavior ◽  
Neural Mechanism ◽  
Distal Colon ◽  
Full Length ◽  
Propagating Waves ◽  
Mouse Small Intestine ◽  
Small Intestinal ◽  
Small And Large Intestine

Cyclical propagating waves of muscle contraction have been recorded in isolated small intestine or colon, referred to here as motor complexes (MCs). Small intestinal and colonic MCs are neurogenic, occur at similar frequencies, and propagate orally or aborally. Whether they can be coordinated between the different gut regions is unclear. Motor behavior of whole length mouse intestines, from duodenum to terminal rectum, was recorded by intraluminal multisensor catheter. Small intestinal MCs were recorded in 27/30 preparations, and colonic MCs were recorded in all preparations ( n = 30) with similar frequencies (0.54 ± 0.03 and 0.58 ± 0.02 counts/min, respectively). MCs propagated across the ileo-colonic junction in 10/30 preparations, forming “full intestine” MCs. The cholinesterase inhibitor physostigmine increased the probability of a full intestine MC but had no significant effect on frequency, speed, or direction. Nitric oxide synthesis blockade by Nω-nitro-l-arginine, after physostigmine, increased MC frequency in small intestine only. Hyoscine-resistant MCs were recorded in the colon but not small intestine ( n = 5). All MCs were abolished by hexamethonium ( n = 18) or tetrodotoxin ( n = 2). The enteric neural mechanism required for motor complexes is present along the full length of both the small and large intestine. In some cases, colonic MCs can be initiated in the distal colon and propagate through the ileo-colonic junction, all the way to duodenum. In conclusion, the ileo-colonic junction provides functional neural continuity for propagating motor activity that originates in the small or large intestine. NEW & NOTEWORTHY Intraluminal manometric recordings revealed motor complexes can propagate antegradely or retrogradely across the ileo-colonic junction, spanning the entire small and large intestines. The fundamental enteric neural mechanism(s) underlying cyclic motor complexes exists throughout the length of the small and large intestine.

scholarly journals Lymphatic absorption of plasmalogen in rats

2003 ◽  
Vol 90 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Hiroshi Hara ◽  
Takuya Wakisaka ◽  
Yoritaka Aoyama
Keyword(s):  
Small Intestine ◽  
Wistar Rats ◽  
Nacl Solution ◽  
Lymphatic Absorption ◽  
Mesenteric Lymph ◽  
Brain Phospholipids ◽  
Male Wistar Rats ◽  
Lymph Duct ◽  
Fatty Aldehydes ◽  
Long Chain

Plasmalogen is a subclass of phospholipids that is widely distributed in man and animals. Many physiological roles have been proposed for this lipid; however, there have been no reports on the intestinal absorption of plasmalogen. In the present study, we examined lymphatic absorption of plasmalogen after the duodenal infusion of emulsified brain phospholipids (BPL) containing plasmalogen (22 mol% of total phospholipids) and soyabean lecithin (SPL) (100 g emulsified phospholipid/l). Male Wistar rats with implanted cannulas in the mesenteric lymph duct and the duodenum were kept in a Bollman-type restraining cage, and were infused the emulsion after 1 d recovery with duodenal infusion of a glucose–NaCl solution. Lymphatic plasmalogen output was increased at 2–4 h after the switch to BPL emulsion, and peaked at 4–6 h. However, no increases were observed after SPL infusion. Lymphatic recovery of plasmalogen for 8 h was 198 nmol, which was 0·22 mol% of the total plasmalogen disappeared from the intestine. We did not detect any increases in long-chain fatty aldehydes, which are the degradation product of plasmalogen, either in the blood or the small intestine. We conclude that a small percentage but a significant amount of the plasmalogen was absorbed into the lymph.

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