255 EFFECT OF THE TYPE OF DIETARY FATTY ACID (α-LINOLENIC ACID OR LINOLEIC ACID) ON THE NUMBER AND THE QUALITY OF OOCYTES COLLECTED BY OVUM PICKUP AND ON IN VITRO EMBRYO PRODUCTION BY DAIRY HEIFERS

2007 ◽  
Vol 19 (1) ◽  
pp. 244 ◽  
Author(s):  
A. A. Ponter ◽  
J. Arnault ◽  
C. Joly ◽  
K. Guelou ◽  
G. Valy ◽  
...  
Keyword(s):  
Growth Rate ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Dietary Fat ◽  
Dietary Treatment ◽  
Embryo Production ◽  
Experimental Conditions

A supplement of dietary fat can improve oocyte quality in ruminants. However, to our knowledge, the effect of the type dietary fat (differing in fatty acid profile) on oocyte and embryo production has never been reported in cattle. Therefore, in this study the effect of the type of fat supplement on the number and quality of oocytes collected by ovum pickup (OPU) and on the production of embryos was investigated in Holstein heifers. The experiment was conducted over 2 years: year 1: 8 heifers, and year 2: 10 heifers (16 to 20 months old, body weight 368 � 8.2 kg and BCS 2.3 � 0.1). Heifers were given a diet of hay (67% DM) and a concentrate (33% DM). The concentrate (130 g fat/kg DM) was formulated with either extruded linseeds (L, rich in α-linolenic acid (ω-3 fatty acid),n = 9) or extruded soybeans (S, rich in linoleic acid (ω-6 fatty acid), n = 9). Oocytes were collected by OPU for 6 weeks (2 sessions/week) and their morphological quality was assessed (Q1, excellent; Q2, good; Q3, fair; and Q4, low). The oocytes from one session/week were frozen and those from the other session were used to produce embryos (in vitro maturation and fertilization). Embryo quality was judged following IETS criteria (EQ1, excellent; EQ2, good; EQ3, fair; and EQ4, low). Blood samples were collected weekly throughout the experiment for the analysis of insulin, insulin-like growth factor-1, glucose, non-esterified fatty acids, β-hydroxybutyrate, and urea. Statistical analysis was performed on the growth rate, BCS, hormone, metabolite, and fatty acid data and on the oocyte and embryo data using Split plot ANOVA. Growth rate was not affected by diet (S, 0.93 � 0.21 kg d-1 vs. L, 0.90 � 0.21 kg d-1), and there was no effect of dietary treatment on plasma hormone and metabolite concentrations. However, treatment L increased the proportion of α-linolenic acid (P < 0.0001), and treatment S increased the proportion of linoleic acid (P < 0.0001) in the plasma. Neither the oocyte characteristics (number of oocytes collected, 5.3 � 1.0 vs. 5.7 � 1.0; their quality: Q1, 1.1 � 0.3 vs. 1.0 � 0.3; Q2, 1.8 � 0.3 vs. 1.6 � 0.3; Q3, 1.4 � 0.4 vs. 2.1 � 0.4; and Q4, 1.0 � 0.2 vs. 1.1 � 0.2; and oocytes inseminated, 4.4 � 0.8 vs. 5.1 � 0.8, and cleaved, 3.4 � 0.7 vs. 3.5 � 0.7 per heifer per session; L vs. S, respectively) nor the embryo characteristics (number of embryos, 1.1 � 0.3 vs. 0.9 � 0.3; their quality: EQ1, 0.4 � 0.1 vs. 0.3 � 0.1; EQ2, 0.4 � 0.1 vs. 0.3 � 0.1; EQ3, 0.2 � 0.1 vs. 0.3 � 0.1; and EQ4, 0.04 � 0.03 vs. 0.05 � 0.03 per heifer per session; L vs. S, respectively) were affected by dietary treatment. In conclusion, under our experimental conditions, the type of fatty acid (ω-3 vs. ω-6) does not modify significantly the numbers of oocytes and embryos produced by OPU and their quality in dairy eifers.

208 SUPEROVULATORY RESPONSE AND EMBRYO PRODUCTION IN HOLSTEIN COWS FED DIETS ENRICHED IN OLEIC, LINOLEIC, OR α-LINOLENIC ACID

2014 ◽  
Vol 26 (1) ◽  
pp. 218
Author(s):  
R. Salehi ◽  
M. G. Colazo ◽  
A. Behrouzi ◽  
A. Ruiz-Sanchez ◽  
M. Oba ◽  
...  
Keyword(s):  
Oleic Acid ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Embryonic Development ◽  
Linolenic Acid ◽  
Early Embryonic Development ◽  
Holstein Cows ◽  
Corpora Lutea ◽  
Embryo Production

Early embryonic development is enhanced in Holstein cows fed diets enriched in linoleic and α-linolenic acids compared to those fed a diet enriched in stearic fatty acid (Thangavelu et al. 2007; Theriogenology 68, 949–957). A positive effect of oleic acid on oocyte competence in vitro has been reported (Aardema et al. 2011; Biol. Reprod. 85, 62–69), but the influence of oleic acid on early embryonic development has not been investigated. Therefore, our objective was to evaluate the effects of diets enriched in oleic, linoleic, or α-linolenic acid on superovulatory response and embryo production. Non-lactating Holstein cows were blocked by parity and bodyweight, and assigned to one of three diets supplemented with canola (CAN, n = 8; 62% oleic acid), sunflower (SUN, n = 7; 73% linoleic acid), or flax (FLX, n = 8; 57% α-linolenic acid). Cows were individually offered hay (9.4 kg) and concentrate mixture (4.1 kg) supplemented with one of the three rolled oilseeds (~8% of dietary dry matter). After a minimum of 21 days of diet adaptation, cows were given FSH (300 mg total dose, eight 12-hourly injections), artificially inseminated twice, 12 h apart, with semen of the same sire and ova/embryos recovered non-surgically 7.5 days post-insemination. Once started on a diet, cows continued to receive the same diet until the end of the experiment. Thirty-five collections (flushing) were performed (CAN: 12, SUN: 11 and FLX: 12) and data were analysed using the GENMOD procedure of SAS (SAS Institute Inc., Cary, NC, USA); results are presented as mean ± s.e. Number of corpora lutea (12.8 ± 2.6, 14.5 ± 1.2 and 13.1 ± 1.5), anovulated follicles (3.0 ± 1.4, 2.0 ± 1.1 and 2.6 ± 0.9), and total ova/embryos (7.5 ± 2.0, 8.6 ± 1.7 and 7.3 ± 1.2) did not differ (P > 0.05) for CAN, SUN, and FLX treatment, respectively. Cows fed SUN tended (P = 0.06) to produce more transferable embryos (6.1 ± 1.7) than those fed CAN (4.3 ± 1.5), but did not differ from those fed FLX (4.8 ± 1.3). There were fewer (P < 0.05) unfertilized ova in cows fed SUN (0.6 ± 0.5) than in those fed either CAN (1.3 ± 0.6) or FLX (1.8 ± 0.9). Conversely, cows fed FLX had fewer (P < 0.05) degenerated embryos (0.7 ± 0.4) compared to those fed either CAN (1.9 ± 0.6) or SUN (1.9 ± 0.5). The number of expanded blastocysts was greater (P < 0.05) in cows fed CAN (1.08 ± 0.57) than in those fed FLX (0.25 ± 0.25), but it was not different from those fed SUN (0.90 ± 0.36). In summary, the type of dietary fatty acid did not affect superovulatory response, but a diet enriched in oleic acid resulted in more expanded blastocysts, whereas a diet enriched in linoleic acid significantly reduced the number of unfertilized ova and numerically increased transferable embryos. The diet enriched in α-linolenic acid resulted in fewer degenerated embryos. This study was supported by Alberta Meat and Livestock Agency, Alberta Milk, and Alberta Innovates-BioSolutions.

scholarly journals Explore the gene network regulating the composition of fatty acids in cottonseed

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Lihong Ma ◽  
Xinqi Cheng ◽  
Chuan Wang ◽  
Xinyu Zhang ◽  
Fei Xue ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Gene Network ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Accumulation Pattern ◽  
Time Points ◽  
Expression Characteristics

Abstract Background Cottonseed is one of the major sources of vegetable oil. Analysis of the dynamic changes of fatty acid components and the genes regulating the composition of fatty acids of cottonseed oil is of great significance for understanding the biological processes underlying biosynthesis of fatty acids and for genetic improving the oil nutritional qualities. Results In this study, we investigated the dynamic relationship of 13 fatty acid components at 12 developmental time points of cottonseed (Gossypium hirsutum L.) and generated cottonseed transcriptome of the 12 time points. At 5–15 day post anthesis (DPA), the contents of polyunsaturated linolenic acid (C18:3n-3) and saturated stearic acid (C18:0) were higher, while linoleic acid (C18:2n-6) was mainly synthesized after 15 DPA. Using 5 DPA as a reference, 15,647 non-redundant differentially expressed genes were identified in 10–60 DPA cottonseed. Co-expression gene network analysis identified six modules containing 3275 genes significantly associated with middle-late seed developmental stages and enriched with genes related to the linoleic acid metabolic pathway and α-linolenic acid metabolism. Genes (Gh_D03G0588 and Gh_A02G1788) encoding stearoyl-ACP desaturase were identified as hub genes and significantly up-regulated at 25 DPA. They seemed to play a decisive role in determining the ratio of saturated fatty acids to unsaturated fatty acids. FAD2 genes (Gh_A13G1850 and Gh_D13G2238) were highly expressed at 25–50 DPA, eventually leading to the high content of C18:2n-6 in cottonseed. The content of C18:3n-3 was significantly decreased from 5 DPA (7.44%) to 25 DPA (0.11%) and correlated with the expression characteristics of Gh_A09G0848 and Gh_D09G0870. Conclusions These results contribute to our understanding on the relationship between the accumulation pattern of fatty acid components and the expression characteristics of key genes involved in fatty acid biosynthesis during the entire period of cottonseed development.

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

Gamolenic acid in atopic eczema: ▾ epogam

1990 ◽  
Vol 28 (18) ◽  
pp. 69-70
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Essential Fatty Acid ◽  
Atopic Eczema ◽  
Conventional Treatment ◽  
Gamma Linolenic Acid ◽  
Evening Primrose

Epogam capsules (Scotia) contain oil from the seed of the evening primrose which is rich in the essential fatty acid linoleic acid and its metabolite gamolenic (gamma-linolenic) acid. Epogam is licensed for use to relieve symptoms in atopic eczema, and is claimed to act at a fundamental metabolic level in this disease. Conventional treatment of eczema is often unsatisfactory, and any claim of an advance must be taken seriously. How well founded are the claims made for Epogam?

OPTIMIZATION OF COCOA BUTTER EQUIVALENT PRODUCTION FROM FORMULATED HARD PALM OIL MID-FRACTION AND CANOLA OIL BLENDS

2016 ◽  
Vol 78 (6-12) ◽  
Author(s):  
Reiza Mutia ◽  
Dayang Norulfairuz Abang Zaidel ◽  
Ida Idayu Muhamad
Keyword(s):  
Fatty Acid ◽  
Linoleic Acid ◽  
Reaction Time ◽  
Cocoa Butter ◽  
Linolenic Acid ◽  
Palm Oil ◽  
Canola Oil ◽  
Cocoa Butter Equivalent ◽  
Omega 3 ◽  
Omega 6

The study to find cocoa butter equivalent (CBE) as an alternative to cocoa butter (CB) from available and low cost commercial oils or fats has been increased recently. Current study investigates the blending of hard palm oil mid-fraction (PMF) with canola oil to produce high nutritional CBE using immobilized lipase from Rhizomucor miehei. The experiments were designed using Response Surface Methodology (RSM) to optimize the percentage of saturated-unsaturated-saturated (StUSt) triacylglycerols (TAGs). The experiment was performed at hard PMF concentration of 50 to 90% (w/w), lipozyme load between 5% and 10% (based on the weight of substrate) with a reaction time between 2 to 14 hours. The best reaction conditions to attain this target was 89.35% (w/w) of hard PMF concentration, 2 hours of reaction time, and 5% (based on the weight of substrate) of lipozyme load, resulting CBE which contains 64.44±1.18% of StUSt. The addition of canola oil improved the nutritional value of CBE which was marked by the higher percentage of linoleic acid (omega-6, 4.53±0.06%) and linolenic acid (omega-3, 0.74±0.14%) in CBE than CB (omega-6, 2.68±0.34%). Enzymatic interesterification was not altering fatty acid content in the CBE, especially linoleic acid (omega-6) and linolenic acid (omega-3) which was characterized by no significant difference (p > 0.05) between the fatty acid profile of initial mixture (before interesterification) and CBE (after interesterification).

Effects of feeding ryegrass of varying maturity on the metabolism and composition of lipids in the rumen of sheep

1986 ◽  
Vol 106 (3) ◽  
pp. 445-448 ◽  
Author(s):  
T. Gerson ◽  
A. John ◽  
A. S. D. King
Keyword(s):  
Fatty Acid ◽  
Lipid Metabolism ◽  
Linoleic Acid ◽  
Blood Plasma ◽  
Polyunsaturated Fatty Acid ◽  
Perennial Ryegrass ◽  
Crude Protein ◽  
Linseed Oil ◽  
Triacyl Glycerol

SummaryTwo experiments were carried out to test the effects of ryegrass maturity on rumen lipid metabolism. In the first experiment the effect of stage of maturity of perennial ryegrass on lipid metabolism in the rumen was studied with grazing sheep fitted with rumen cannulae. The pasture was either immature (13·8% crude protein), mature (8·1% crude protein) or senescent (5·5% crude protein).The ratesin vitroof triacyl glycerol lipolysis and linoleic acid (18: 2w6) hydrogenation were found to decrease with increasing age of the ryegrass.In the second experiment the sheep were dosed with emulsified linseed oil (30 g) via rumen cannulae while grazing immature or senescent ryegrass and the rumen digesta and blood plasma sampled at 0, 4 and 8 h after dosing.The proportions of linseed oil retained in the rumen were greater and blood plasma linoleic (18:2w6) and linolenic (18:3w3) acid concentrations higher when senescent ryegrass was fed.It was concluded that the rates of rumen lipolysis and hydrogenation decreased with the age of pasture and that after dosing with linseed oil the polyunsaturated fatty acid concentrations in blood plasma increased.

scholarly journals Fatty acid chemistry of Atrichum undulatum and Hypnum andoi

2012 ◽  
Vol 66 (2) ◽  
pp. 207-209 ◽  
Author(s):  
Boris Pejin ◽  
Ljubodrag Vujisic ◽  
Marko Sabovljevic ◽  
Vele Tesevic ◽  
Vlatka Vajs
Keyword(s):  
Fatty Acids ◽  
Gas Chromatography ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Stearic Acid ◽  
Palmitic Acid ◽  
Linolenic Acid ◽  
Essential Fatty Acids ◽  
Behenic Acid ◽  
Moss Species

The fatty acid composition of the moss species Atrichum undulatum (Hedw.) P. Beauv. (Polytrichaceae) and Hypnum andoi A.J.E. Sm. (Hypnaceae) collected in winter time were analyzed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) as a contribution to their chemistry. Eight fatty acids were identified in the chloroform/methanol extract 1:1 of A. undulatum (linoleic acid 26.80%, palmitic acid 22.17%, ?-linolenic acid 20.50%, oleic acid 18.49%, arachidonic acid 6.21%, stearic acid 3.34%, cis-5,8,11,14,17-eicosapentaenoic acid 1.52% and behenic acid 1.01%), while six fatty acids were found in the same type of extract of H. andoi (palmitic acid 63.48%, erucic acid 12.38%, stearic acid 8.08%, behenic acid 6.26%, lignoceric acid 5.16% and arachidic acid 4.64%). According to this study, the moss A. undulatum can be considered as a good source of both essential fatty acids for humans (linoleic acid and ?-linolenic acid) during the winter.

scholarly journals The Effect of Walnut Flour on the Physical and Sensory Characteristics of Wheat Bread

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Noha M. Almoraie
Keyword(s):  
Linoleic Acid ◽  
Physical Properties ◽  
Linolenic Acid ◽  
Proximate Composition ◽  
Specific Volume ◽  
Sensory Quality ◽  
Wheat Bread ◽  
Loaf Volume ◽  
Standard Procedures

The study was carried out to demonstrate the effect of walnut flour enhancement on the physical, nutritional, and sensory quality of bread. Walnut flour was prepared by soaking, deshelling, oven drying, and sieving whole walnuts. The wheat flour was supplemented with walnut flour by 0, 20, 30, 40, and 50% of the total amount. Standard procedures were taken to estimate the proximate composition of wheat and walnut flour and bread samples. A comparison between the control and supplemented bread was made, where the physical characteristics (weight, volume, and specific volume) and sensory quality were checked. The enhanced bread, where the percentage was between 20 and 50%, appeared to have a significant increase in protein, fat, linoleic acid, and α-linolenic acid and a decrease in carbohydrate and fibre values. Increased walnut flour replacement showed that physical properties, loaf volume and specific loaf volume, have declined. The sensory attributes between the unsupplemented and supplemented bread showed major differences. As an outcome, substituting 30% walnut flour gave the best overall quality of bread acceptability.

scholarly journals Recovery of Fatty Acid Composition in Mediterranean Yellowtail (Seriola dumerili, Risso 1810) fed a Fish-Oil Finishing Diet

2020 ◽  
Vol 21 (14) ◽  
pp. 4871
Author(s):  
Francesco Bordignon ◽  
Silvia Martínez-Llorens ◽  
Angela Trocino ◽  
Miguel Jover-Cerdá ◽  
Ana Tomás-Vidal
Keyword(s):  
Arachidonic Acid ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Docosahexaenoic Acid ◽  
Eicosapentaenoic Acid ◽  
Fish Oil ◽  
Linolenic Acid ◽  
Vegetable Oils ◽  
Fa Composition

The present study evaluated the effects of wash-out on the fatty acid (FA) composition in the muscles of Mediterranean yellowtail. After 109 days during which fish were fed either a fish oil (FO)-based diet (FO 100) or a diet (FO 0) in which FO was completely substituted by vegetable oils, all fish were subjected to a wash-out with FO 100 diet for 90 days. The FA profile of muscles in fish fed FO 0 diet at the beginning of the experiment reflected that of dietary vegetable oils, rich in linoleic acid (LA), and α-linolenic acid (ALA), and was deficient in AA (arachidonic acid), EPA (eicosapentaenoic acid), and DHA (docosahexaenoic acid). No essential FA were fully restored in fish previously fed FO 0 diet on 45th or 90th day of wash-out. At the end of wash-out, the FA composition showed that AA, EPA, and DHA in the white muscles increased by +33%, +16%, and +43% (p < 0.001), respectively. Similarly, AA and DHA in the red muscles increased by +33% and +41% respectively, while EPA remained similar to fish fed FO 0 diet exclusively. Therefore, a 90-d wash-out can partially improve the FA profile in muscles of Mediterranean yellowtail previously fed vegetable oil-based diets.

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