Residues of famphur in bovine tissues and milk following its application as a pour-on insecticide

1976 ◽  
Vol 16 (78) ◽  
pp. 82 ◽  
Author(s):  
AM Annand ◽  
JHP Dingle ◽  
AB Heath ◽  
WA Palmer
Keyword(s):  
Body Weight ◽  
Topical Application ◽  
Half Life ◽  
Subcutaneous Fat ◽  
Maximum Level ◽  
Post Mortem ◽  
The Third ◽  
Whole Milk ◽  
Liver And Kidney ◽  
Residue Levels

Three experiments were conducted to determine residues of famphur in tissues and milk of cattle following its topical application. Subcutaneous fat, sampled by biopsy, from animals treated at 150 mg famphur per kg body weight contained maximum residues of famphur (about 10 mg kg-1, average) one day after treatment. Levels of treatment at 50 mg kg-1 and 25 mg kg-1 yielded similar but lower residues after the same period (2.08 and 1.8 p.p.m, respectively). The half-life of famphur residues was independent of the initial residue levels and was calculated as 0.9 day. Mean residues were negligible (highest mean 0.08 p.p.m.) by five days after treatment. Post-mortem sampling of cattle treated with famphur at 45 mg kg-1 showed that at one day and seven days after treatment, residues in fats (up to 1.25 p.p.m. and 0.53 p.p.m. respectively) and muscle (1.41 p.p.m. and 0.71 p.p.m. respectively) were similar but were higher than the negligible levels (0.05 p.p.m. or less) found in liver and kidney. By 14 days, levels in all tissues were very low (0.11 p.p.m. or less). In milk from cows treated with 23 mg famphur kg-1, 76 per cent of the famphur was found in the butterfat and a maximum level (0.237 p.p.m.) in whole milk was found in the first milking after treatment. Residues were negligible (0.008 p.p.m.) by the third day.

Once-daily administration of CJC-1295, a long-acting growth hormone-releasing hormone (GHRH) analog, normalizes growth in the GHRH knockout mouse

2006 ◽  
Vol 291 (6) ◽  
pp. E1290-E1294 ◽  
Author(s):  
Maria Alba ◽  
Danilo Fintini ◽  
Alessia Sagazio ◽  
Betty Lawrence ◽  
Jean-Paul Castaigne ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Body Weight ◽  
Half Life ◽  
Subcutaneous Fat ◽  
Daily Administration ◽  
Good Growth ◽  
Duration Of Action ◽  
Once Daily ◽  
Releasing Hormone ◽  
Normal Body

Although the majority of children with isolated growth hormone (GH) deficiency have a good growth response to GH-releasing hormone (GHRH), the use of this therapeutic agent is limited by its very short half-life. Indeed, we have shown that, in mice with GHRH gene ablation (GHRH knockout; GHRHKO), even twice-daily injections of a GHRH analog are unable to normalize growth. CJC-1295 is a synthetic GHRH analog that selectively and covalently binds to endogenous albumin after injection, thereby extending its half-life and duration of action. We report the effects of CJC-1295 administration in GHRHKO animals. Three groups of 1-wk-old GHRHKO mice were treated for 5 wk with 2 μg of CJC-1295 at intervals of 24, 48, and 72 h. Placebo-treated GHRHKO mice and mice heterozygous for the GHRHKO allele served as controls. GHRHKO animals receiving daily doses of CJC-1295 exhibited normal body weight and length. Mice treated every 48 and 72 h reached higher body weight and length than placebo-treated animals, without full growth normalization. Femur and tibia length remained normal in animals treated every 24 and 48 h. Relative lean mass and subcutaneous fat mass were normal in all treated groups. CJC-1295 caused an increase in total pituitary RNA and GH mRNA, suggesting that proliferation of somatotroph cells had occurred, as confirmed by immunohistochemistry images. These findings demonstrate that treatment with once-daily administration of CJC-1295 is able to maintain normal body composition and growth in GHRHKO mice. The same dose is less effective when administered every 48 or 72 h.

scholarly journals Post-mortem Redistribution of Alprazolam in Rats

2020 ◽  
Vol 121 (4) ◽  
pp. 244-253
Author(s):  
Jana Hořínková ◽  
Petr Kozlík ◽  
Tomáš Křížek ◽  
Danica Michaličková ◽  
Martin Šíma ◽  
...  
Keyword(s):  
Rat Model ◽  
Half Life ◽  
Sampling Time ◽  
Post Mortem ◽  
Tissue Concentrations ◽  
Tissue Samples ◽  
Liver And Kidney ◽  
Continuous Accumulation ◽  
Median Amount

The post-mortem toxicological findings may be misinterpreted, if the drug undergoes substantial post-mortem redistribution. As alprazolam is one of the most frequently evaluated drug for legal/forensic reasons in drug-related fatalities, we studied possible changes in alprazolam distribution after death in a rat model. Rats were sacrificed 30 minutes after alprazolam administration. Blood and tissue samples from 8 animals per sampling time were collected at 0, 2, 6, and 24 h after death. The experimental samples were assayed for alprazolam using validated UHPLC-PDA method. Median blood alprazolam concentrations increased approximately 2 times compared with ante-mortem levels due to the redistribution during early post-mortem phase and then slowly decreased with a half-life of 60.7 h. The highest alprazolam tissue concentrations were found in fat and liver and the lowest levels were observed in lungs and brain. The median amount of alprazolam deposited in the lungs was relatively stable over the 24-h post-mortem period, while in heart, liver and kidney the deposited proportion of administered dose increased by 43–48% in comparison with ante-mortem values indicating continuous accumulation of alprazolam into these tissues. These results provide evidence needed for the interpretation of toxicological results in alprazolam-related fatalities and demonstrate modest alprazolam post-mortem redistribution.

scholarly journals PASTURE FOR YOUNG DAIRY STOCK: II. EFFECT OF PREVIOUS NUTRITIONAL LEVEL AND AGE ON GROWTH OF HOLSTEIN HEIFER CALVES ON PASTURE

1961 ◽  
Vol 41 (1) ◽  
pp. 63-70
Author(s):  
Vern Miles ◽  
V. S. Logan ◽  
W. J. Pigden
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Skim Milk ◽  
Live Weight ◽  
Feeding Period ◽  
Live Weight Gain ◽  
The Third ◽  
Whole Milk ◽  
Nutritional Level ◽  
Treatment Comparisons

Thirty-five Holstein heifer calves were assigned at birth to three ration schedules: moderate m; control c; high h. These three groups were weaned from whole milk during the 2nd, 7th and 9th weeks respectively with m and h calves receiving skim milk to 7 and 16 weeks of age respectively. m and c calves were allowed 4 pounds and h calves up to 6 pounds of meal daily with no limit on hay consumption. Moderate and high rationed calves were further sub-divided into three birth groups and were abruptly switched from the above feeding schedules and placed on pasture without supplementation at 56, 112, and 168 days. Treatment comparisons included live weight gain, withers height and heart girth measurements.High rationed calves made greater growth increases than m or c calves during the barn feeding period. These differences were greater for weight than for heart girth or withers increases. Pastured calves made smaller body gains during the first 28-day pasture period than barn-fed controls. This trend was reversed by the third pasture period with some pastured calves gaining over 2 pounds daily, h calves placed on pasture at 2 months of age made greater body weight gains and appeared to be thriftier than calves previously moderately rationed.

scholarly journals Influence of the administration of propolis and bee pollen preparation on the concentration of mercury in the muscles, kidneys and liver of white storks

2018 ◽  
Vol 74 (3) ◽  
pp. 171-174 ◽  
Author(s):  
Jose L. Valverde Piedra ◽  
Agata Zienkiewicz ◽  
Hussein B. Khudhur ◽  
Sylwia E. Szymańczyk ◽  
Ewa Rumińska
Keyword(s):  
Body Weight ◽  
Mercury Concentration ◽  
Wild Birds ◽  
Post Mortem ◽  
Rehabilitation Center ◽  
Broken Bones ◽  
White Storks ◽  
Liver And Kidney ◽  
Wing Damage ◽  
One Year

A total of ninety white storks (Ciconia ciconia) of both sexes aged over one year of life and at a body weight between 2.8 – 4.15kg were subjects for observations. They were collected from the Warmia and Masuria region, and were rehabilitees of The Wild Birds Rehabilitation Center (Bukwald, Poland). The storks formed a group of birds that had wing damage like broken bones and were unable to fly. According to the severity of the case storks underwent three different kinds of treatment. Light cases of motion disability were submitted to wing or leg stabilization with adhesive bandages (treatment I), while middle and severe cases were additionally submitted to the administration of one (treatment II) or two capsules (treatment III) of propolis and pollen bee preparation (Apipol Farma’s Propolis Plus®) for two weeks, respectively. After the convalescence period a total of twenty three white storks did not recover and were euthanized and dissected. Post mortem samples of pectoral and femoral muscles as well as liver and kidney samples were taken. Mercury concentration was analyzed and the results revealed that the level in the kidneys and liver of white storks not receiving propolis preparation were significantly higher than that of those from treatment II and III. Contrary to this, the mercury concentration recorded in the pectoral and femoral muscles of the birds of treatment II and treatment III were significantly higher than that of the treatment without propolis preparation. The results showed that propolis and pollen bee preparation can reduce the level of mercury in kidneys and liver, but has no influence on the reduction of mercury in pectoral and femoral muscles. .

Residues of chlordimeform in bovine tissues and milk following application by a handspray

1977 ◽  
Vol 17 (86) ◽  
pp. 380
Author(s):  
WA Palmer ◽  
JHP Dingle ◽  
AB Heath
Keyword(s):  
Half Life ◽  
Liver Tissue ◽  
Subcutaneous Fat ◽  
Residue Level ◽  
Cattle Tick ◽  
Spray Application ◽  
Tissue Concentrations ◽  
Lactating Cows ◽  
Whole Milk

Three experiments were conducted to determine the concentrations of residues of chlordimeform in tissues and milk of cattle after spray application to control cattle tick. Subcutaneous fat, sampled by biopsy from animals sprayed with 0.45 per cent (w/v), 0.15 per cent and 0.05 per cent chlordimeform (buffered) contained maximum residues of chlordimeform (2.88 mg kg-1, 0.46 mg kg-1 and 0.1 5 mg kg-1 respectively) one day after treatment. The half life for the rate of disappearance of these residues was independent of the initial residue level and was calculated as 2.46 days. Sampling of six tissues, 24 hours after spraying with chlordimeform (buffered) showed that chlordimeform was found mainly in fat. Smaller concentrations were found in kidney, muscle and liver tissue. Concentrations of 0.45 per cent, 0.15 per cent and 0.05 per cent chlordimeform (buffered) produced residues of 1.42 mg kg-1, 0.28 mg kg-1 and 0.03 mg kg-1 respectively in the whole milk of lactating cows. A half life of 0.45 day was calculated for the rate of disappearance of chlordimeform from the milk.

scholarly journals Efficacy of UK-109496, a new azole antifungal agent, in an experimental model of invasive aspergillosis.

1996 ◽  
Vol 40 (1) ◽  
pp. 86-91 ◽  
Author(s):  
D George ◽  
P Miniter ◽  
V T Andriole
Keyword(s):  
Body Weight ◽  
Invasive Aspergillosis ◽  
Aspergillus Fumigatus ◽  
Antifungal Agent ◽  
Half Life ◽  
Oral Therapy ◽  
Rabbit Model ◽  
Circulating Antigen ◽  
Liver And Kidney ◽  
Tissue Burden

The efficacy of UK-109496, a new azole antifungal agent, was evaluated in an immunosuppressed, temporarily leukopenic rabbit model of invasive aspergillosis. Oral therapy with UK-109496 at a dosage of 10 or 15 mg/kg of body weight every 8 h was begun 24 h after a lethal or sublethal challenge, and results were compared with those for amphotericin B therapy and untreated controls. UK-109496 eliminated mortality and also reduced the tissue burden of Aspergillus fumigatus 10- to 100-fold in liver and kidney tissues and to a lesser degree in lung tissue, and at the higher dose, no viable organisms were recovered from brain tissue from these animals. Both dosages of UK-109496 decreased or eliminated circulating antigen. The half-life of UK-109496 in rabbits was 2.5 to 3 h, and no accumulation of drug was seen even after 15 doses in either uninfected or infected animals. Thus, UK-109496 shows activity in this rabbit model of invasive aspergillosis. Additional studies are needed to determine the potential of the drug for use in the treatment of this infection.

Turnover and Distribution of 131Iodine-Labelled Human Fibrinogen

1964 ◽  
Vol 11 (02) ◽  
pp. 404-422 ◽  
Author(s):  
Annemarie Amris ◽  
C. J Amris
Keyword(s):  
Body Weight ◽  
Cancer Patient ◽  
Distribution Ratio ◽  
Half Life ◽  
Fibrinolytic Activity ◽  
The Other ◽  
Turnover Rates ◽  
Human Fibrinogen ◽  
Coagulation Defects ◽  
Fractional Turnover

Summary14 patients (5 diabetics with arteriosclerotic complications, 4 patients with thrombo-embolic disease, 4 with cirrhosis, coagulation defects and increased fibrinolytic activity, and 1 cancer patient) and 3 control patients were subjected to turnover studies with 13iodine labelled human fibrinogen.Half-life times in the control patients were found to be 4 days, the fractional turnover rates 19–23 per cent, of intravascular fibrinogen per day, and the absolute turnover 0.02 to 0.06 gm per day per kg. body weight. The other patient’s half-life times and turnover rates varied considerably from 0.9–5.5 days, 13–160 per cent, per day of intravascular fibrinogen and 0.02–0.4 gm per day per kg. body weight respectively.As fibrinogen unlike other proteins subjected to turnover studies, is converted to fibrin, it is not possible to measure the true intra-extravascular distribution ratio of fibrinogen. But intravascular fibrinogen could be approximated to constitute 68–99 per cent, of the total fibrinogen. There is justification in believing that fibrinogen is degradated through a continuous coagulation in equilibrium with fibrinolysis, and that the organism contains a greater mass of fibrin, the “fibrin pool”. Considerations of the turnover mechanism can however only be hypothetical.

scholarly journals Nucleotide Supplementation to Whole Milk Has Beneficial Effects on Post-Weaning Holstein Calf Performance

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 218
Author(s):  
Yousef Abbaslou ◽  
Davood Zahmatkesh ◽  
Ehsan Mahjoubi ◽  
Mehdi Hossein Yazdi ◽  
Hamed Beiranvand ◽  
...  
Keyword(s):  
Body Weight ◽  
Average Daily Gain ◽  
Free Access ◽  
Treatment Groups ◽  
Beneficial Effects ◽  
Positive Effects ◽  
Whole Milk ◽  
Calf Performance ◽  
Daily Gain ◽  
Fecal Score

The positive effects of nucleotide (NU) supplementation in milk replacer have been elucidated in infants and in dairy calves; however, NU addition to whole milk has not been evaluated previously. This study aimed to assess NU supplementation in the whole milk on calf growth and health. Thirty Holstein calves (body weight: 39.1 ± 1.0 kg; 3 d after birth) were randomly assigned to the following treatments: whole milk without any supplementation (NU0), whole milk + 0.5 g/d added a NU-containing supplement to whole milk (NUCS0.5), and whole milk + 1 g/d added a NU-containing supplement to whole milk (NUCS1). Calves were weaned at d 55 and stayed on study until d 75. Calves had free access to feed and water throughout the study. Dry matter intakes (DMI) were similar among treatments (p > 0.05) during the pre-weaning period; however, increasing NU resulted in a linear (p < 0.05) increase in DMI during the post weaning period (2158, 2432, and 2518 g/d for NU0, NUCS0.5, and NUCS1, respectively). Treatments did not affect body weight (BW) at the first and second month of study, but final BW linearly increased as NU was added (87.1, 90.6, and 95.4 kg for NU0, NUCS0.5, and NUCS1, respectively). Neither pre-weaning average daily gain nor post-weaning average daily gain was affected by treatments; accordingly, feed efficiency was similar among treatment groups. Days with loose fecal score were linearly decreased as NU was added to whole milk during the first month of life, while the fecal score did not differ among treatments until the end of the study. No difference was observed in the skeletal growth of calves in the current study. Therefore, it can be concluded that NU supplementation in the whole milk has some beneficial effects on calf performance in terms of final BW, post-weaning DMI, and less days with loose feces.

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