Enzymatic transamination: The synthesis of α,β,β-Trideutero-L-glutamic acid and α-Dentero-L-glutamic acid

1969 ◽  
Vol 22 (8) ◽  
pp. 1779 ◽  
Author(s):  
DJ Whelan ◽  
GJ Long
Keyword(s):  
Glutamic Acid ◽  
Pyruvic Acid ◽  
Oxaloacetic Acid ◽  
Glutamate Pyruvate

α,β,β-Trideutero-L-glutamic acid has been synthesized by incubating L- glutamic acid in D2O at pD 7.8 in the presence of pig-heart glutamate- oxaloacetate aminotransferase and catalytic amounts of pyridoxal 5?- phosphate and oxaloacetic acid. However, when L-glutamic acid is incubated under similar conditions with pig-heart glutamate-pyruvate aminotransferase, in the presence of catalytic amounts of pyridoxal 5?- phosphate and pyruvic acid, α-deutero-L-glutamic acid is obtained.

scholarly journals Biological synthesis of oxaloacetic acid from pyruvic acid and carbon dioxide

1940 ◽  
Vol 34 (10-11) ◽  
pp. 1383-1395 ◽  
Author(s):  
H. A. Krebs ◽  
L. V. Eggleston
Keyword(s):  
Carbon Dioxide ◽  
Pyruvic Acid ◽  
Biological Synthesis ◽  
Oxaloacetic Acid

CHANGES IN GLUTAMIC ACID – OXALOACETIC ACID – TRANSAMINASE ACTIVITY IN THE LIVER AND KIDNEYS ON THE EFFECT OF HIGH DOSES OF PYRAMIDON (AMINOPYRINE)

1961 ◽  
Vol 39 (7) ◽  
pp. 1189-1191 ◽  
Author(s):  
I. Gy. Fazekas ◽  
Á. Gy. Fazekas ◽  
B. Rengei
Keyword(s):  
Glutamic Acid ◽  
Lethal Dose ◽  
Transaminase Activity ◽  
Renal Cells ◽  
Oxaloacetic Acid ◽  
High Doses

On each of 5 days, rabbits were given subcutaneously 75% of the single lethal dose of Pyramidon (aminopyrine), divided into three daily doses. The animals were killed on the 6th day and the degree of glutamic acid – oxaloacetic acid – transaminase activity of the liver and kidneys was examined. It could be established that activity in the liver increased by 41% and that of the kidneys by 87%. These values are correlated with the lesions which were previously noted in these organs. The increased seral activity as well as the ratio of activity of the organs of the treated and untreated animals is attributed to increased permeability and necrosis in hepatic and renal cells.

scholarly journals Changes in Pyruvic Acid Content and GPT Activity in Chilling-sensitive and Nonsensitive Crops

HortScience ◽  
1990 ◽  
Vol 25 (8) ◽  
pp. 952-953 ◽  
Author(s):  
Hironobu Tsuchida ◽  
Cheng Dan-Hong ◽  
Kazuko Inoue ◽  
Nobuyuki Kozukue ◽  
Susumu Mizuno
Keyword(s):  
Cucumis Sativus ◽  
Cold Storage ◽  
Pyruvic Acid ◽  
Daucus Carota ◽  
Acid Content ◽  
Common Factor ◽  
Solanum Melongena ◽  
Glutamate Pyruvate Transaminase ◽  
Glutamate Pyruvate

This study investigated the mechanism of alanine accumulation observed as a common factor in chilling-sensitive crops [cucumber (Cucumis sativus L.) and eggplant (Solanum melongena L.)] during cold storage. Pyruvic acid in acids increased rapidly during the storage of these crops at 1C. However, in sensitive crops at 20C or in a chilling-resistant crop [carrot (Daucus carota L.)] at 1C, the increase was not found. No significant changes in glutamate-pyruvate transaminase (GPT) activity closely related to the biosynthesis of alanine were found in chilling-sensitive or chilling-resistant crops during storage at 1, 5, or 20C. We suggest that the accumulated alanine found in chilling-sensitive crops may be produced by GPT from accumulated pyruvic acid during cold storage.

scholarly journals The production of pyruvic acid, oxaloacetic acid and α-oxoglutaric acid from glucose by tissue in culture

1959 ◽  
Vol 72 (4) ◽  
pp. 619-623 ◽  
Author(s):  
G. A. Abdel-Tawab ◽  
E. Broda ◽  
G. Kellner
Keyword(s):  
Pyruvic Acid ◽  
Oxaloacetic Acid

scholarly journals Biosynthesis of food constituents: Amino acids: 1. The glutamic acid and aspartic acid groups – a review

2011 ◽  
Vol 24 (No. 1) ◽  
pp. 1-10
Author(s):  
J. Velíšek ◽  
K. Cejpek
Keyword(s):  
Amino Acids ◽  
Citric Acid ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Citric Acid Cycle ◽  
Acid Group ◽  
Review Article ◽  
Acid Cycle ◽  
Oxaloacetic Acid ◽  
Reaction Schemes

This review article gives a survey of principal pathways that lead to the biosynthesis of the proteinogenic amino acids of the glutamic acid group (glutamic acid, glutamine, proline, arginine) and aspartic acid group (aspartic acid, asparagine, threonine, methionine, lysine, isoleucine) starting with oxaloacetic acid from the citric acid cycle. There is an extensive use of reaction schemes, sequences, and mechanisms with the enzymes involved and detailed explanations using sound chemical principles and mechanisms.

STUDIES ON DIACETYL BIOSYNTHESIS BY STREPTOCOCCUS DIACETILACTIS

1963 ◽  
Vol 9 (4) ◽  
pp. 431-441 ◽  
Author(s):  
Eugene W. Seitz ◽  
W. E. Sandine ◽  
P. R. Elliker ◽  
E. A. Day
Keyword(s):  
Citric Acid ◽  
Pyruvic Acid ◽  
Acid Decarboxylase ◽  
Cell Free Extract ◽  
Oxaloacetic Acid ◽  
Resting Cell ◽  
Butanediol Dehydrogenase

Resting cell and cell-free extract experiments demonstrated the presence of citritase, oxaloacetic acid decarboxylase, α-acetolactic acid decarboxylase, and pyruvic acid decarboxylase in Streptococcus diacelilactis. Results indicated that citric acid or pyruvic acid was converted to diacetyl and acetylmethylcarbinol with intermediate synthesis of α-acetolactic acid. Acetylmethylcarbinol also was synthesized by condensation of active and free acetaldehyde. Evidence was obtained for the presence of diacetyl reductase and a reversible 2,3-butanediol dehydrogenase in cell-free extracts of S. diacetilactis. The data suggested that S. diacetilactis produced diacetyl from citrate with the intermediate production of oxaloacetate, pyruvate, and α-acetolactate in that order.

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