scholarly journals Deep Freezing of Angora Goat Semen: Effects of Diluent Composition and Method and Rate of Dilution on Survival of Spermatozoa

1982 ◽  
Vol 35 (3) ◽  
pp. 295 ◽  
Author(s):  
S Salamon ◽  
AJ Ritar

Five factorial experiments were conducted to examine the effects of concentration of tris(hydroxymethyl) aminomethane (Tris), type and concentration of sugar in the diluent, rate and method of dilution on the survival of goat spermatozoa after freezing by the pellet method.

1965 ◽  
Vol 18 (4) ◽  
pp. 901 ◽  
Author(s):  
ICA Martin

From the results of four factorial experiments on the deep-freezing of bull spermatozoa: (1) Revival rates of semen treated with lecithin followed by cooling to 5�C with dilution just before freezing did not differ significantly from samples diluted at 30�C soon after collection of the ejaculate. Aging the spermatozoa at 5�C for 6 hr before freezing was beneficial and a slow freezing rate of O� 5 to 1 degC fall per minute to -15�C followed by 3 degC fall below this temperature gave better results than faster rates. Time of storage at 5�C and freezing rate interacted, as fast freezing was much better tolerated by spermatozoa which had been aged at 5�C for 6 or 18 hr.


1974 ◽  
Vol 27 (5) ◽  
pp. 485 ◽  
Author(s):  
D Visser ◽  
S Salamon

Six factorial experiments were conducted to examine the effects of concentrations of 2-amino-2-hydroxymethylpropane-l,3-diol (tris), sugars, erythritol, catalase, ethylenediaminetetra-acetic acid (EDTA), glycerol and egg yolk in the freezing diluent on the survival of boar spermatozoa after freeze-thawing.


1968 ◽  
Vol 21 (2) ◽  
pp. 351 ◽  
Author(s):  
S Salamon

Four factorial experiments were conducted in which were examined and compared factors which affect revival of ram spermatozoa after pellet.freezing and other methods of freezing. Revival of spermatozoa after pellet.freezing was the best in egg yolk-lactose (333 mM), followed by egg yolk-raffinose (333 mM), egg yolk-glucose (355 mM), and egg yolk-fructose (355 mM) diluents. Within the glycerol range of 0-7%, lactose gave the best results with 3%, raffinose and glucose with 5%, and fructose with 7% (vJv) glycerol.


1973 ◽  
Vol 26 (1) ◽  
pp. 231 ◽  
Author(s):  
I Wilmut ◽  
S Salamon ◽  
C Polge

Five factorial experiments were conducted to examine the effects of glycerol concentration and processing procedures prior to freezing on the revival of boar spermatozoa upon thawing.


1973 ◽  
Vol 26 (1) ◽  
pp. 239 ◽  
Author(s):  
S Salamon

Five factorial experiments were conducted to examine the effects of centrifugation of semen, diluent and dilution rate, pellet volume, and method of thawing on the survival of boar spermatozoa after freezing by the pellet method.


1973 ◽  
Vol 26 (1) ◽  
pp. 219 ◽  
Author(s):  
S Salamon ◽  
I Wilmut ◽  
C Polge

A series of factorial experiments was conducted to examine the effects of sugars and polyols (inositol, dulcitol), glycerol, low molecular weight polyols and cell "non-permeating" agents as cryoprotectives, and method of thawing on survival of boar spermatozoa after freezing by the pellet method.


Author(s):  
Jaspa Samwel ◽  
Theodosy Msogoya ◽  
Abdul Kudra ◽  
Hosea Dunstan Mtui ◽  
Anna Baltazari ◽  
...  

Abstract Background Orange (Citrus sinensis L.) production in Tanzania is constrained by several pre-harvest factors that include pests. Hexanal, sprayed as Enhanced Freshness Formulation (EFF) is a relatively new technology that has been reported to reduce pre-harvest loss in fruits. However, the effects of hexanal on pre-harvest yield loss of orange are not known. We studied the effects of hexanal as EFF on yield losses of three sweet orange cultivars namely, Early Valencia, Jaffa, and Late Valencia. Factorial experiments tested the effects of EFF concentration, variety, and time of EFF application on number of dropped fruit, percentage of non-marketable fruit and incidence of pest damage. Results Results showed significant negative correlation (p < 0.001) between EFF and the percentage of dropped fruit, non-marketable yield, and incidence of pest damage. An increase in hexanal concentration by 1%, is expected to reduce number of dropped fruit by 50, percentage of non-marketable by 35.6, and incidences of pest damage by 36.5% keeping other factors constant. Results also show significant association (p < 0.001) between time of hexanal application and non-marketable yield. Percentage of dropped fruit is expected to increase by 1 for each day away from harvest, keeping other factors constant. Conclusion Pre-harvest application of hexanal as EFF can significantly reduce number of dropped fruits, percentage of non-marketable fruit and incidence of pest damage.


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