Deep Freezing of Boar Semen III. Effects of Centrifugation, Diluent and Dilution Rate, Pellet Volume, and Method of Thawing on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 239 ◽  
Author(s):  
S Salamon
Keyword(s):  
Dilution Rate ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

Five factorial experiments were conducted to examine the effects of centrifugation of semen, diluent and dilution rate, pellet volume, and method of thawing on the survival of boar spermatozoa after freezing by the pellet method.

scholarly journals Deep Freezing of Boar Semen II. Effects of Method of Dilution, Glycerol Concentration, and Time of Semen-Glycerol Contact on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 231 ◽  
Author(s):  
I Wilmut ◽  
S Salamon ◽  
C Polge
Keyword(s):  
Glycerol Concentration ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

Five factorial experiments were conducted to examine the effects of glycerol concentration and processing procedures prior to freezing on the revival of boar spermatozoa upon thawing.

scholarly journals Deep Freezing of Boar Semen I. Effects of Diluent Composition, Protective Agents, and Method of Thawing on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 219 ◽  
Author(s):  
S Salamon ◽  
I Wilmut ◽  
C Polge
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Factorial Experiments ◽  
Protective Agents ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

A series of factorial experiments was conducted to examine the effects of sugars and polyols (inositol, dulcitol), glycerol, low molecular weight polyols and cell "non-permeating" agents as cryoprotectives, and method of thawing on survival of boar spermatozoa after freezing by the pellet method.

Effect of Composition of Tris-based Diluent on Survival of Boar Spermatozoa following Deep Freezing

1974 ◽  
Vol 27 (5) ◽  
pp. 485 ◽  
Author(s):  
D Visser ◽  
S Salamon
Keyword(s):  
Acetic Acid ◽  
Egg Yolk ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Spermatozoa

Six factorial experiments were conducted to examine the effects of concentrations of 2-amino-2-hydroxymethylpropane-l,3-diol (tris), sugars, erythritol, catalase, ethylenediaminetetra-acetic acid (EDTA), glycerol and egg yolk in the freezing diluent on the survival of boar spermatozoa after freeze-thawing.

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Tao Shen ◽  
Zhong-Liang Jiang ◽  
Cong-Jun Li ◽  
Xiao-Chen Hu ◽  
Qing-Wang Li
Keyword(s):  
Lipoic Acid ◽  
Artificial Insemination ◽  
Antioxidant Effect ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Alpha Lipoic Acid ◽  
Glutamic Oxaloacetic Transaminase ◽  
Boar Semen ◽  
Endogenous Antioxidant ◽  
Boar Spermatozoa

SummaryAlpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing–thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen–thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing–thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

Influence of Equilibration, Freezing Rate, Method of Dilution, and Diluent on the Survival of Deep-Frozen Bull Spermatozoa

1965 ◽  
Vol 18 (4) ◽  
pp. 901 ◽  
Author(s):  
ICA Martin
Keyword(s):  
Freezing Rate ◽  
Slow Freezing ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Rate Method ◽  
Fast Freezing

From the results of four factorial experiments on the deep-freezing of bull spermatozoa: (1) Revival rates of semen treated with lecithin followed by cooling to 5�C with dilution just before freezing did not differ significantly from samples diluted at 30�C soon after collection of the ejaculate. Aging the spermatozoa at 5�C for 6 hr before freezing was beneficial and a slow freezing rate of O� 5 to 1 degC fall per minute to -15�C followed by 3 degC fall below this temperature gave better results than faster rates. Time of storage at 5�C and freezing rate interacted, as fast freezing was much better tolerated by spermatozoa which had been aged at 5�C for 6 or 18 hr.

scholarly journals Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1930
Author(s):  
Eva Tvrdá ◽  
Mégane Debacker ◽  
Michal Ďuračka ◽  
Ján Kováč ◽  
Ondřej Bučko
Keyword(s):  
Mitochondrial Activity ◽  
Ros Generation ◽  
Dna Integrity ◽  
Membrane Stabilization ◽  
New Information ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
The Impact ◽  
Sample Dilution

In this study, we evaluated the impact of 5–50 μM quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p < 0.001 and p < 0.0001; 10 μM), and which correlated with the most prominent maintenance of sperm motility (p < 0.01, 48 h; p < 0.05, 72 h). A significant membrane stabilization (p < 0.01, 24 h and 48 h; p < 0.0001, 72 h) and prevention of lipid peroxidation (p < 0.05, 24 h and 48 h; p < 0.01, 72 h) was primarily observed following administration of 10 and 25 μM NAR; respectively. Administration of 10 μM QUE led to a significant decrease of superoxide (p < 0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 μM NAR (p < 0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.

scholarly journals Cryopreservation of boar semen

2020 ◽  
Vol 65 (No. 4) ◽  
pp. 115-123
Author(s):  
Marija Jovičić ◽  
Eva Chmelíková ◽  
Markéta Sedmíková
Keyword(s):  
Animal Species ◽  
Semen Quality ◽  
Egg Yolk ◽  
High Rate ◽  
Freezing And Thawing ◽  
Long Term Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
Term Storage

Sperm cryopreservation is the best technology for long-term storage of the semen. However, the damage of boar spermatozoa by cryopreservation is more severe than in other animal species and a standardized freezing protocol for efficient cryopreservation has not been established yet. Semen quality and freezability vary greatly between breeds as well as between individual boars and even the season. Boar spermatozoa are sensitive to low temperatures; they sustain damage and a high rate of mortality and freezing/thawing the boar semen may strongly impair the sperm function and decrease the semen quality. The freezability of boar semen can be influenced by a cryopreservation procedure, and also by using various additives to freezing and thawing extenders such as antioxidants. In order to obtain acceptable results after thawing the boar semen, it is necessary to combine an optimal amount of additives (glycerol, egg yolk, sugars, antioxidants), cooling and warming velocities.

scholarly journals Effects of feeding tuna oil on the lipid composition of pig spermatozoa and in vitro characteristics of semen

Reproduction ◽  
2001 ◽  
pp. 315-322 ◽  
Author(s):  
JA Rooke ◽  
CC Shao ◽  
BK Speake
Keyword(s):  
Fatty Acids ◽  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Basal Diet ◽  
Tuna Oil ◽  
Total Fatty Acids ◽  
Boar Semen ◽  
Boar Spermatozoa

The aim of the present study was to characterize the effects of feeding tuna oil on the lipid and fatty acid composition of boar spermatozoa and to relate changes in composition to boar semen characteristics. Ten boars were paired by age and allocated to one of two diets (five boars per diet). The diets, which were offered for 6 weeks, consisted of a basal diet that was either unsupplemented or supplemented with 30 g tuna oil kg(-1) diet. Adding tuna oil to the diet increased the ether extract concentration of the diets fed from 65 to 92 g kg(-1) dry matter and supplied 10.5 g long chain polyunsaturated (n-3) fatty acids per 100 g total fatty acids. There were no changes in semen fatty acid composition after 3 weeks of feeding tuna oil. However, after 5 and 6 weeks, the proportions (g per 100 g total fatty acids) of 22:6(n-3) in sperm phospholipid fatty acids were increased from 34.5 to 42.9 g by feeding tuna oil and 22:5(n-6) decreased from 29.8 to 17.9 g. No changes were observed in other sperm lipids or seminal plasma phospholipids as a result of the diets fed. Feeding tuna oil increased the proportion of spermatozoa with progressive motility and with a normal acrosome score and reduced the proportion of spermatozoa with abnormal morphologies.

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