scholarly journals Deep Freezing of Boar Semen II. Effects of Method of Dilution, Glycerol Concentration, and Time of Semen-Glycerol Contact on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 231 ◽  
Author(s):  
I Wilmut ◽  
S Salamon ◽  
C Polge
Keyword(s):  
Glycerol Concentration ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

Five factorial experiments were conducted to examine the effects of glycerol concentration and processing procedures prior to freezing on the revival of boar spermatozoa upon thawing.

Deep Freezing of Boar Semen III. Effects of Centrifugation, Diluent and Dilution Rate, Pellet Volume, and Method of Thawing on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 239 ◽  
Author(s):  
S Salamon
Keyword(s):  
Dilution Rate ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

Five factorial experiments were conducted to examine the effects of centrifugation of semen, diluent and dilution rate, pellet volume, and method of thawing on the survival of boar spermatozoa after freezing by the pellet method.

scholarly journals Deep Freezing of Boar Semen I. Effects of Diluent Composition, Protective Agents, and Method of Thawing on Survival of Spermatozoa

1973 ◽  
Vol 26 (1) ◽  
pp. 219 ◽  
Author(s):  
S Salamon ◽  
I Wilmut ◽  
C Polge
Keyword(s):  
Molecular Weight ◽  
Low Molecular Weight ◽  
Factorial Experiments ◽  
Protective Agents ◽  
Deep Freezing ◽  
Boar Semen ◽  
Boar Spermatozoa

A series of factorial experiments was conducted to examine the effects of sugars and polyols (inositol, dulcitol), glycerol, low molecular weight polyols and cell "non-permeating" agents as cryoprotectives, and method of thawing on survival of boar spermatozoa after freezing by the pellet method.

Effect of Composition of Tris-based Diluent on Survival of Boar Spermatozoa following Deep Freezing

1974 ◽  
Vol 27 (5) ◽  
pp. 485 ◽  
Author(s):  
D Visser ◽  
S Salamon
Keyword(s):  
Acetic Acid ◽  
Egg Yolk ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Boar Spermatozoa

Six factorial experiments were conducted to examine the effects of concentrations of 2-amino-2-hydroxymethylpropane-l,3-diol (tris), sugars, erythritol, catalase, ethylenediaminetetra-acetic acid (EDTA), glycerol and egg yolk in the freezing diluent on the survival of boar spermatozoa after freeze-thawing.

INFLUENCE OF GLYCEROL CONCENTRATION AND FREEZING TO −79 C ON OXYGEN UPTAKE AND LIVABILITY OF BOAR AND BULL SPERMATOZOA

1972 ◽  
Vol 52 (1) ◽  
pp. 65-72 ◽  
Author(s):  
L. M. SANFORD ◽  
G. J. KING ◽  
J. W. MACPHERSON
Keyword(s):  
Oxygen Uptake ◽  
Incubation Period ◽  
Skim Milk ◽  
Egg Yolk ◽  
Freezing Process ◽  
Glycerol Concentration ◽  
Motile Cells ◽  
Boar Semen ◽  
Boar Spermatozoa

Boar and bull spermatozoa were diluted in a skim milk–egg yolk–glucose extender containing 0, 7.5, or 15% glycerol (v/v) and incubated aerobically for 6 hr at 37 C. Other partially diluted boar semen samples were cooled to 5 C. Glycerol was added to a final concentration of 0, 7.5, and 15%. Samples were frozen to −79 C, rewarmed, and incubated for 3 hr at 37 C. The presence of glycerol in the extender depressed (P < 0.01) the oxygen uptake by nonfrozen boar and bull spermatozoa during the 6-hr incubation period. The reduction of oxygen uptake by semen samples increased as the level of glycerol in the extender increased. There was a corresponding decrease (P < 0.01) in the number of motile cells at the conclusion of the incubation period. Glycerol appeared to have more of a detrimental effect on boar spermatozoan oxygen uptake. The rate of oxygen uptake by boar semen samples postfreezing was extremely depressed, suggesting that spermatozoa surviving the freezing process metabolize at a much lower rate than normal. Active progressive motility of most of the surviving boar spermatozoa ceased within 1–2 hr of incubation under the in vitro conditions of this experiment.

scholarly journals Examination of Some Processing Methods for Freezing Boar Semen

1976 ◽  
Vol 29 (4) ◽  
pp. 325 ◽  
Author(s):  
O Osinowo ◽  
S Salamon
Keyword(s):  
Seminal Plasma ◽  
Glycerol Concentration ◽  
Processing Methods ◽  
Freezing Method ◽  
Freezing Medium ◽  
Boar Semen ◽  
Cell Concentrations ◽  
Boar Spermatozoa ◽  
Medium Method

Five experiments were conducted to examine the effect of processing methods and diluents on survival and morphology of boar spermatozoa after freezing. Post-thawing survival of spermatozoa was better for Beltsville-F3 (BF3) than for tris-fructoseEDT A freezing diluent when the seminal plasma and glycerol were removed prior to freezing (method A). Both freezing diluents yielded similar viability results when the spermatozoa were frozen in the presence of seminal plasma and glycerol (method B). Viability of spermatozoa after thawing was better when glycerol concentration in the prefreezing diluent (method A) or in the freezing medium (method B) was 2� 5 and 5� 0 rather than 7� 5 %. Cooling of diluted semen to 5�C beyond 4 h decreased the post-thawing survival of spermatozoa. The proportion of spermatozoa with undamaged acrosomes after processing and thawing by different methods was indistinguishable and relatively low. When the semen was frozen at cell concentrations ranging from 0�25 to 2�0 x 109/mI, the viability of spermatozoa declined with increasing concentration following freezing in BF3 and S-1 diluents. Viability results were very similar for all cell concentrations examined when tris-fructose-EDTA diluent was used, indicating the possibility of freezing boar semen in a concentrated state.

scholarly journals Binding of boar spermatozoa to oviductal epithelium in vitro in relation to sperm morphology and storage time

Reproduction ◽  
2006 ◽  
Vol 131 (2) ◽  
pp. 311-318 ◽  
Author(s):  
D Waberski ◽  
F Magnus ◽  
F Ardón ◽  
A M Petrunkina ◽  
K F Weitze ◽  
...  
Keyword(s):  
Semen Quality ◽  
Binding Capacity ◽  
Storage Period ◽  
Sperm Function ◽  
Sperm Binding ◽  
Oviductal Epithelium ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa

In vitro short-term storage of boar semen for up to 72 h before insemination negatively affects fertility, but this often remains undetected during semen quality assessment. One important sperm function is the ability to form the functional sperm reservoir in the oviduct. In the present study, we used the modified oviductal explant assay to study sperm binding to oviductal epithelium in vitro in diluted boar semen stored for 24 or 72 h. First, we determined the kinetics of in vitro sperm binding to oviductal epithelium in relation to co-incubation time of sperm and oviductal tissue pieces. Then, we studied how the binding of sperm to oviductal epithelium was affected by in vitro semen storage and by differences among individual boars. Sperm binding after different incubation times was significantly higher when semen was stored 24 h than after 72-h storage (P < 0.05), and peaked at 30–90 min of incubation. Sperm binding differed between boars (n = 44), and was negatively correlated to the percentage of sperm with cytoplasmic droplets (R = −0.51, P < 0.001). There were no significant changes in motility, acrosome integrity and propidium iodide stainability during the 72-h storage period. However, sperm-binding indices were significantly lower after 72 h in vitro storage than after 24-h storage in sperm from boars with normal semen quality (P < 0.05); in contrast, the binding capacity of sperm from boars with higher percentages of morphologically altered sperm remained at a low level. The sperm-binding capacity of sperm from four of the five boars with known subfertility was lower than the mean binding index minus one standard deviation of the boar population studied here. It is concluded that changes in the plasma membrane associated with in vitro ageing reduce the ability of stored boar sperm to bind to the oviductal epithelium. This study shows the potential of sperm–oviduct binding as a tool to assess both male fertility and changes in sperm function associated with in vitro ageing.

Effect of alpha-lipoic acid on boar spermatozoa quality during freezing–thawing

Zygote ◽  
2015 ◽  
Vol 24 (2) ◽  
pp. 259-265 ◽  
Author(s):  
Tao Shen ◽  
Zhong-Liang Jiang ◽  
Cong-Jun Li ◽  
Xiao-Chen Hu ◽  
Qing-Wang Li
Keyword(s):  
Lipoic Acid ◽  
Artificial Insemination ◽  
Antioxidant Effect ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Alpha Lipoic Acid ◽  
Glutamic Oxaloacetic Transaminase ◽  
Boar Semen ◽  
Endogenous Antioxidant ◽  
Boar Spermatozoa

SummaryAlpha-lipoic acid (ALA) is known to be a natural antioxidant. The aim of the present study was to evaluate the cryoprotective effect of ALA on the motility of boar spermatozoa and its antioxidant effect on boar spermatozoa during freezing–thawing. Different concentrations (2.0, 4.0, 6.0, 8.0 or 10.0 mg/ml) of ALA were added to the extender used to freeze boar semen, and the effects on the quality and endogenous antioxidant enzyme activities of frozen–thawed spermatozoa were assessed. The results indicated that the addition of ALA to the extender resulted in a higher percentage of motile spermatozoa post-thaw (P < 0.05). The activities of superoxide dismutase, lactate dehydrogenase, glutamic-oxaloacetic transaminase and catalase improved after adding ALA to the extender (P < 0.05). Artificial insemination results showed that pregnancy rate and litter size were significantly higher at 6.0 mg/ml in the ALA group than in the control group (P < 0.05). In conclusion, ALA conferred a cryoprotective capacity to the extender used for boar semen during the process of freezing–thawing, and the optimal concentration of ALA for the frozen extender was 6.0 mg/ml.

Influence of Equilibration, Freezing Rate, Method of Dilution, and Diluent on the Survival of Deep-Frozen Bull Spermatozoa

1965 ◽  
Vol 18 (4) ◽  
pp. 901 ◽  
Author(s):  
ICA Martin
Keyword(s):  
Freezing Rate ◽  
Slow Freezing ◽  
Factorial Experiments ◽  
Deep Freezing ◽  
Rate Method ◽  
Fast Freezing

From the results of four factorial experiments on the deep-freezing of bull spermatozoa: (1) Revival rates of semen treated with lecithin followed by cooling to 5�C with dilution just before freezing did not differ significantly from samples diluted at 30�C soon after collection of the ejaculate. Aging the spermatozoa at 5�C for 6 hr before freezing was beneficial and a slow freezing rate of O� 5 to 1 degC fall per minute to -15�C followed by 3 degC fall below this temperature gave better results than faster rates. Time of storage at 5�C and freezing rate interacted, as fast freezing was much better tolerated by spermatozoa which had been aged at 5�C for 6 or 18 hr.

scholarly journals Quercetin and Naringenin Provide Functional and Antioxidant Protection to Stored Boar Semen

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1930
Author(s):  
Eva Tvrdá ◽  
Mégane Debacker ◽  
Michal Ďuračka ◽  
Ján Kováč ◽  
Ondřej Bučko
Keyword(s):  
Mitochondrial Activity ◽  
Ros Generation ◽  
Dna Integrity ◽  
Membrane Stabilization ◽  
New Information ◽  
Semen Storage ◽  
Boar Semen ◽  
Boar Spermatozoa ◽  
The Impact ◽  
Sample Dilution

In this study, we evaluated the impact of 5–50 μM quercetin (QUE) and naringenin (NAR) on extended boar spermatozoa in the BTS (Beltsville Thawing Solution) medium for 72 h. Spermatozoa motion, membrane, acrosome, and DNA integrity were investigated immediately after sample dilution (0 h) as well as after 24 h, 48 h, and 72 h of semen storage. Furthermore, reactive oxygen species (ROS) and superoxide production, as well as the extent of oxidative damage to the sperm proteins and lipids, were assessed to determine the potential of QUE and NAR to prevent a potential loss of sperm vitality due to oxidative stress development. Our results indicate that the most notable parameter influenced by QUE was the mitochondrial activity, which remained significantly higher throughout the experiment (p < 0.001 and p < 0.0001; 10 μM), and which correlated with the most prominent maintenance of sperm motility (p < 0.01, 48 h; p < 0.05, 72 h). A significant membrane stabilization (p < 0.01, 24 h and 48 h; p < 0.0001, 72 h) and prevention of lipid peroxidation (p < 0.05, 24 h and 48 h; p < 0.01, 72 h) was primarily observed following administration of 10 and 25 μM NAR; respectively. Administration of 10 μM QUE led to a significant decrease of superoxide (p < 0.0001, 48 h and 72 h) while the most notable decline of ROS generation was recorded in the case of 10 and 25 μM NAR (p < 0.001). This study may provide new information on the specific mechanisms of action involved in the favorable effects of natural biomolecules on spermatozoa.

Sign in / Sign up

Export Citation Format

Share Document