scholarly journals The Extracellular Polysaccharide of a Methylotrophic Culture

1978 ◽  
Vol 31 (3) ◽  
pp. 311 ◽  
Author(s):  
MN Huq ◽  
BJ Ralph ◽  
PAD Rickard
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Extracellular Polysaccharide ◽  
Molar Ratios ◽  
O 31

The constituent sugars of the extracellular polysaccharide produced by an enrichment culture, growing on methane as sole carbon source, were identified as glucose, galactose, mannose, fucose and rhamnose, in the approximate molar ratios of 1�00: o� 36: 0�19 : O� 31 : 0�16. When the culture was grown on methanol as sole carbon source, only glucose, galactose and mannose, in the approximate molar ratios of 1 �00 : 0�67 : 0�42, were identified as components of the extracellular polysaccharide.

scholarly journals Isolation and Characterization of Biosurfactant-producing Alcaligenes sp. YLA11 and its Diesel Degradation Potentials

2021 ◽  
Vol 9 (2) ◽  
pp. 7-12
Author(s):  
Abdulrahman Abdulhamid Arabo ◽  
Raji Arabi Bamanga ◽  
Mujiburrahman Fadilu ◽  
Musa Abubakar ◽  
Fatima Abdullahi Shehu ◽  
...  
Keyword(s):  
Carbon Source ◽  
Incubation Time ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Diesel Oil ◽  
Contaminated Site ◽  
Screening Methods ◽  
Bacterial Strains ◽  
Isolation And Characterization ◽  
Degrading Bacteria

This study aimed to isolate and identify biosurfactant producing and diesel alkanes degrading bacteria. For this reason, bacteria isolated from the diesel contaminated site were screened for their potential to produce biosurfactants and degrade diesel alkanes. Primary selection of diesel degraders was carried out by using conventional enrichment culture technique where 12 bacterial strains were isolated based on their ability to grow on minimal media supplemented with diesel as sole carbon source, which was followed by qualitative screening methods for potential biosurfactant production. Isolate B11 was the only candidate that shows positive signs for drop collapse, foaming, haemolytic test, oil displacement of more than 22 ± 0.05 mm, and emulsification (E24) of 14 ± 0.30%. The effect of various culture parameters (incubation time, diesel concentration, nitrogen source, pH and temperature) on biodegradation of diesel was evaluated. The optimum incubation time was confirmed to be 120 days for isolates B11, the optimum PH was confirmed as 8.0 for the isolate, Similarly, the optimum temperature was confirmed as 35oC. In addition, diesel oil was used as the sole carbon source for the isolates. The favourable diesel concentration was 12.5 % (v/v) for the isolate. The isolate has shown degradative ability towards Tridecane (C13), dodecane, 2, 6, 10-trimethyl- (C15), Tetradecane (C14), 2,6,10-Trimethyltridecane (C16), Pentadecane (C15). It degraded between 0.27% - 9.65% individual diesel oil alkanes. The strain has exhibited the potential of degrading diesel oil n-alkanes and was identified as Alcaligenes species strain B11 (MZ027604) using the 16S rRNA sequencing.

scholarly journals Glyphosate Utilization as the Source of Carbon: Isolation and Identification of new Bacteria

2011 ◽  
Vol 8 (4) ◽  
pp. 1582-1587 ◽  
Author(s):  
M. Mohsen Nourouzi ◽  
T. G. Chuah ◽  
Thomas S. Y. Choong ◽  
C. J. Lim
Keyword(s):  
Carbon Source ◽  
Stenotrophomonas Maltophilia ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Bacterial Isolates ◽  
Bacterial Strains ◽  
Isolation And Identification ◽  
Providencia Alcalifaciens ◽  
Mixed Bacteria ◽  
Aminomethyl Phosphonic Acid

Mixed bacteria from oil palm plantation soil (OPS) were isolated to investigate their ability to utilize glyphosate as carbon source. Results showed that approximately all of the glyphosate was converted to aminomethyl-phosphonic acid (AMPA) (99.5%). It is worthy to note that mixed bacteria were able to degrade only 2% of AMPA to further metabolites. Two bacterial strainsi.e. Stenotrophomonas maltophiliaandProvidencia alcalifacienswere obtained from enrichment culture. Bacterial isolates were cultured individually on glyphosate as a sole carbon source. It was observed that both isolates were able to convert glyphosate to AMPA.

scholarly journals Glyceraldehyde-3-phosphate dehydrogenase of Xanthomonas campestris pv. campestris is required for extracellular polysaccharide production and full virulence

Microbiology ◽  
2009 ◽  
Vol 155 (5) ◽  
pp. 1602-1612 ◽  
Author(s):  
Guang-Tao Lu ◽  
Jia-Ri Xie ◽  
Lei Chen ◽  
Jiang-Ru Hu ◽  
Shi-Qi An ◽  
...  
Keyword(s):  
Carbon Source ◽  
Xanthomonas Campestris ◽  
Sole Carbon Source ◽  
Extracellular Polysaccharide ◽  
Open Reading Frame ◽  
Deficient Mutant ◽  
Wild Type ◽  
Reading Frame ◽  
Phosphofructokinase Activity ◽  
Xanthomonas Campestris Pv Campestris

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) plays an important role in glucose catabolism, converting glyceraldehyde 3-phosphates to 1,3-bisphosphoglycerates. Open reading frame (ORF) XC_0972 in the genome of Xanthomonas campestris pv. campestris (Xcc) strain 8004 is the only ORF in this strain annotated to encode a GAPDH. In this work, we have demonstrated genetically that this ORF encodes a unique GAPDH in Xcc strain 8004, which seems to be constitutively expressed. A GAPDH-deficient mutant could still grow in medium with glucose or other sugars as the sole carbon source, and no phosphofructokinase activity was detectable in strain 8004. These facts suggest that Xcc may employ the Entner–Doudoroff pathway, but not glycolysis, to utilize glucose. The mutant could not utilize pyruvate as sole carbon source, whereas the wild-type could, implying that the GAPDH of Xcc is involved in gluconeogenesis. Furthermore, inactivation of the Xcc GAPDH resulted in impairment of bacterial growth and virulence in the host plant, and reduction of intracellular ATP and extracellular polysaccharide (EPS). This reveals that GAPDH is required for EPS production and full pathogenicity of Xcc.

Extracellular polysaccharide of Aspergillus flavus

1981 ◽  
Vol 46 (10) ◽  
pp. 2435-2440 ◽  
Author(s):  
Alžbeta Kardošová ◽  
Jozef Rosík
Keyword(s):  
Carbon Source ◽  
Aspergillus Flavus ◽  
Sole Carbon Source ◽  
Culture Medium ◽  
Main Chain ◽  
Extracellular Polysaccharide ◽  
Water Soluble ◽  
Molar Weight ◽  
Soluble Polysaccharide ◽  
Hydrolysis Of

A crude extracellular polysaccharide was isolated by precipitation with ethanol from the culture medium of Aspergillus flavus, where the sole carbon source was D-galactose; purification afforded a homogeneous water-soluble polysaccharide in 0.05% yield on the weight of the employed carbon source. This polysaccharide had the relative molar weight 55 000 and [α]D22 -4.2° (c 0.5, H2O); upon total hydrolysis it afforded D-mannose and D-galactose in a 1 : 0.44 ratio. The products of hydrolysis of the methylated polysaccharide and also the course of partial acid and enzymic hydrolyses of the polysaccharide showed that the main chain was formed by (1 → 2) β-linked D-mannose units, of which each second, on average, was substituted by monomeric D-galactose units at C(6).

A Flavobacterium sp. that degrades diazinon and parathion

1973 ◽  
Vol 19 (7) ◽  
pp. 873-875 ◽  
Author(s):  
N. Sethunathan ◽  
T. Yoshida
Keyword(s):  
Carbon Dioxide ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Enrichment Culture ◽  
Culture Technique ◽  
Mineral Medium ◽  
Paddy Water

A Flavobacterium sp., isolated from paddy water by enrichment culture technique, decomposed diazinon in a mineral medium as sole carbon source. The bacterium readily hydrolyzed diazinon to 2-isopropyl-6-methyl-4-hydroxy-pyrimidine which was then converted to carbon dioxide. The bacterium also converted parathion to p-nitrophenol. The enzyme involved in the hydrolysis was constitutive.

scholarly journals Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

2020 ◽  
Author(s):  
Vivek Kumar Ranjan ◽  
Shriparna Mukherjee ◽  
Subarna Thakur ◽  
Krutika Gupta ◽  
Ranadhir Chakraborty
Keyword(s):  
Carbon Source ◽  
Sole Carbon Source ◽  
New Delhi ◽  
Pseudomonas Sp

scholarly journals Study of a plugging microbial consortium using crude oil as sole carbon source

2008 ◽  
Vol 5 (4) ◽  
pp. 367-374 ◽  
Author(s):  
Jing Wang ◽  
Guiwen Yan ◽  
Mingquan An ◽  
Jieli Liu ◽  
Houming Zhang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Crude Oil ◽  
Sole Carbon Source ◽  
Microbial Consortium

scholarly journals Mutants affecting histidine utilization inAspergillus nidulans

1975 ◽  
Vol 25 (2) ◽  
pp. 119-135 ◽  
Author(s):  
Meryl Polkinghorne ◽  
M. J. Hynes
Keyword(s):  
Carbon Source ◽  
Nitrogen Source ◽  
Sole Carbon Source ◽  
Nitrogen Sources ◽  
Limiting Factor ◽  
Sole Nitrogen Source ◽  
Wild Type ◽  
Exogenous Substrate ◽  
Growth Properties ◽  
Type Strains

SUMMARYWild-type strains ofAspergillus nidulansgrow poorly onL-histidine as a sole nitrogen source. The synthesis of the enzyme histidase (EC. 4.3.1.3) appears to be a limiting factor in the growth of the wild type, as strains carrying the mutantareA102 allele have elevated histidase levels and grow strongly on histidine as a sole nitrogen source.L-Histidine is an extremely weak sole carbon source for all strains.Ammonium repression has an important role in the regulation of histidase synthesis and the relief of ammonium repression is dependent on the availability of a good carbon source. The level of histidase synthesis does not respond to the addition of exogenous substrate.Mutants carrying lesions in thesarA orsarB loci (suppressor ofareA102) have been isolated. The growth properties of these mutants on histidine as a sole nitrogen source correlate with the levels of histidase synthesized. Mutation at thesarA andsarB loci also reduces the utilization of a number of other nitrogen sources. The data suggest that these two genes may code for regulatory products involved in nitrogen catabolism. No histidase structural gene mutants were identified and possible explanations of this are discussed.

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