scholarly journals Sulphur Metabolism and Excretion Studies in Ruminants IX. Sulphur, Nitrogen, and Energy Utilization by Sheep Fed a Sulphur-Deficient and a Sulphate-Supplemented, Roughage-Based Diet

1972 ◽  
Vol 25 (5) ◽  
pp. 1073 ◽  
Author(s):  
PR Bird
Keyword(s):  
Energy Utilization ◽  
Sulphur Metabolism ◽  
Treatment Groups ◽  
Basal Ration

Four Merino wethers were each fed a sulphur-deficient, roughage-based ration containing 1'05% nitrogen (79% as urea) and supplying 135 mg sulphur/day. Four other sheep were fed similar amounts of basal ration supplemented with NasS04. This ration supplied 494 mg sulphur/day. Mter 21-day periods the treatment groups were reversed.

scholarly journals Sulphur Metabolism and Excretion Studies in Ruminants V. Ruminal Desulphuration of Methionine and Cyst(e)ine

1972 ◽  
Vol 25 (1) ◽  
pp. 185 ◽  
Author(s):  
PR Bird
Keyword(s):  
Rumen Fluid ◽  
Sulphur Metabolism ◽  
Basal Ration

The concentrations of volatile sulphides in the rumen fluid of sheep were determined at intervals after giving single intraruminal infusions of DL-methionine, L-cystine, or L-cysteine. The basal ration fed contained 0�1 % sulphur.

The effect of delaying the introduction of concentrates on the summer milk production of hay-fed dairy cows

1969 ◽  
Vol 9 (36) ◽  
pp. 66
Author(s):  
JC Radcliffe
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Experimental Period ◽  
Percentage Composition ◽  
Treatment Groups ◽  
Basal Ration ◽  
Friesian Cows

From November 23, 1965, concentrates were introduced successively at two-week intervals to five treatment groups of grade Friesian cows that were being fed a basal ration of cereal hay in the paddock. The yield of milk, butterfat, solids-not-fat, and protein rose significantly as the supplements were introduced but almost no significant effect of concentrate feeding was observed on the weekly percentage composition tests of the milk. A comparison of the total covariance corrected yield results for the experimental period from November 23, 1965, to March 15, 1966, showed that the group of cows receiving concentrates throughout produced 30 per cent more milk, and 29 per cent more solids-not-fat, but only 24 per cent more protein and butterfat than the group receiving concentrates from January 18, 1966. However, the additional milk production would be uneconomic where milk is purchased on a butterfat basis.

Thyroid status and embryonic mortality in swine

1959 ◽  
Vol 196 (2) ◽  
pp. 287-290 ◽  
Author(s):  
George E. Brunstad ◽  
Stewart H. Fowler
Keyword(s):  
Analysis Of Variance ◽  
Embryonic Mortality ◽  
Ovulation Rate ◽  
Thyroid Status ◽  
Thyroid Activity ◽  
Treatment Groups ◽  
The Third ◽  
Altered Thyroid ◽  
And Control ◽  
Basal Ration

Three lots of 10 Palouse gilts each were used to test the effect of altered thyroid activity on embryonic mortality in swine. Two lots received 0.15% thiouracil and 0.0123% thyroprotein, respectively, in a basal ration; the third lot served as the control. All gilts were assigned to treatment groups at first estrus, bred to fertile boars at third estrus and slaughtered 38 days post coitum. An analysis of variance revealed no statistically significant differences among lots for: ovulation rate, embryonic mortality during the first 38 days of gestation, or number of normal embryos at 38 days post coitum. Crown-rump lengths of normal embryos carried by thyroprotein-treated and control gilts were longer than those of normal embryos carried by thiouracil-treated gilts ( P < 0.01). There was a trend toward lowered ovulation rate and reduced embryonic mortality in the gilts receiving thyroprotein. The significance of these results is discussed in relation to BMR and pregnancy in women.

Sulphur metabolism and excretion studies in ruminants. IV. Cystine and sulphate effects upon the flow of sulphur from the rumen and upon sulphur excretion by sheep

1971 ◽  
Vol 22 (3) ◽  
pp. 443 ◽  
Author(s):  
PR Bird ◽  
ID Hume
Keyword(s):  
Latin Square ◽  
Basal Diet ◽  
Sodium Sulphate ◽  
Design Experiment ◽  
Sulphur Metabolism ◽  
Organic Sulphur ◽  
Inorganic Sulphate ◽  
Sulphate Sulphur ◽  
Basal Ration

In a 4 x 4 Latin square design experiment, sheep were fed on a basal ration which supplied 0.61 g sulphur per day, or the basal ration supplemented with 1.4 g inorganic sulphate sulphur, or 1.4 g cystine sulphur, or 1.4 g sulphate sulphur plus 1.4 g cystine sulphur per day. All forms of sulphur supplements increased the daily flow of protein sulphur from the rumen to the omasum (P < 0.05) above basal. Only the cystine treatments increased the flow of sulphide (P < 0.005), ester sulphate (P < 0.005), soluble organic sulphur (P < 0.05), and cystine (P < 0.05), and increased the ruminal sulphide concentrations above basal (P < 0.005). From 7.0 to 12.6% of the added cystine was not degraded in the rumen. In three animals less than 3 % of the added sulphate, alone or in combination with cystine, passed unchanged to the omasum. The fourth animal (67) differed from the others (P < 0.05): the added sulphate was poorly reduced in the rumen, and 62% reached the omasum unchanged. The sulphur composition of the digesta collected from the omasum was: protein sulphur, 55-70%; soluble organic sulphur, 13-22%; inorganic sulphate sulphur (excluding sheep 67), 2.2-2.6 %; ester sulphate sulphur, 1.8-3.4 %; and sulphide, 0.4-28%. Excluding sheep 67, 36% of the dietary sulphur was absorbed from the rumen when the sulphur was added as sodium sulphate or as cystine, and 50 % when added in both forms together. There was a net addition of 0.45 g sulphur per day to the rumen on the basal diet. The influence of the amount and the form of the dietary sulphur on the excretion of faecal and urinary sulphur fractions is discussed.

scholarly journals The influence of ruminal infusion of volatile fatty acids on milk yield and composition and on energy utilization by lactating cows

1969 ◽  
Vol 23 (3) ◽  
pp. 443-453 ◽  
Author(s):  
E. R. Ørskov ◽  
W. P. Flatt ◽  
P. W. Moe ◽  
A. W. Munson ◽  
R. W. Hemken ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Acetic Acid ◽  
Propionic Acid ◽  
Milk Fat ◽  
Volatile Fatty Acids ◽  
Metabolizable Energy ◽  
Energy Utilization ◽  
Acid Infusion ◽  
Body Tissues ◽  
Basal Ration

1. In an experiment of 3 x 3 latin square design, four lactating Holstein cows were given a basal ration designed to induce low percentages of milk fat. The treatments were (I) basal ration, a pelleted mixture of lucerne hay (20%) and concentrates (80%), with 40 l. of water infused intraruminally, (2) basal ration with acetic acid substituted for 15.4% of the metabolizable energy (ME) and (3) propionic acid substituted for 15.4% of the ME. In the last 3 weeks of the 6-week experimental period respiration trials were carried out in an open-circuit indirect calorimeter. The levels of feeding offered in the three periods were 325, 275 and 225 kcal ME/kg body-weight 0.75 in periods 1, 2 and 3 respectively.2. No differences were detected in the utilization of the energy of acetic and propionic acids, but there were differences in the partition of energy into milk or body tissues; with acetic acid infusion more energy was secreted as milk and with propionic acid infusion more was deposited in body tissue.3. There was an increase in milk fat percentage with acetic acid infusion, but not complete recovery to normal. The milk fat percentages were 1.96, 2.58 and 1.92 for treatments 1, 2 and 3 respectively. Acetic acid infusion caused increases in the C12, C14 and C16 fatty acids of milk fat and decreased the proportion of C18:1 fatty acids.4. It is suggested that the low percentages of milk fat found when cows are given concen- trates could result from a decreased extent of fermentation in the rumen, allowing a greater proportion of the starch consumed to be absorbed as glucose in the small intestine.

scholarly journals "Valsyn Mix" In Comparative Studies of the Prevention of Coliform Diarrhea in Calves

1969 ◽  
Vol 50 (4) ◽  
pp. 280-292 ◽  
Author(s):  
José D. Rivera-Anaya ◽  
Carlos M. Berrocal
Keyword(s):  
Body Weight ◽  
Statistical Significance ◽  
Percent Level ◽  
Skim Milk ◽  
Treatment Schedule ◽  
Hematocrit Level ◽  
Treatment Groups ◽  
E Coli ◽  
Newborn Calves ◽  
Basal Ration

A group of 12 newborn calves was fed antibiotic-free reconstituted skim milk, at a 10-percent body weight daily level, for 35 days. Each of two other calf-groups was fed the basal ration with an oxy tot rocy clin e-HCl additive, and the basal ration, with "Vaisyn Mix", a furaltadone preparation. All calves wore orally inoculated for 10 consecutive days with doses of E. coli. Daily temperature and physical observations, and weekly clinico-hematological data were recorded for each calf. Statistical, analyses showed that there were no significant differences between treatment groups in relation to the hematocrit, level and death of calves, or the leukocyte level and death of calves, and also that gain in body-weight of survivor calves is independent of treatment-schedule influence. However, there is statistical significance at the 5-percent level between the ability of Valsyn Mix to protect calves from E. coli diarrhea when compared with oxytetracycline hydrochloride, and no medication.

scholarly journals Sulphur Metabolism and Excretion Studies In Ruminants II. Organic and Inorganic Sulphur Excretion by Sheep After Intraruminal or Intraduodenal Infusions of Sodium Sulphate

1971 ◽  
Vol 24 (4) ◽  
pp. 1329 ◽  
Author(s):  
PR Bird
Keyword(s):  
Sodium Sulphate ◽  
Sulphur Metabolism ◽  
Intraduodenal Infusion ◽  
Inorganic Sulphur ◽  
Basal Ration

A basal ration containing 0�86 g sulphur was fed daily to sheep receiving a continuous intraruminal or intraduodenal infusion supplying 0-6 g sulphur, as sodium sulphate, per day.

Morphological and biochemical analyses of changes in rat hepatocytes related to wine and ethanol consumption

1984 ◽  
Vol 42 ◽  
pp. 232-233
Author(s):  
S.M. Geyer ◽  
C.L. Mendenhall ◽  
J.T. Hung ◽  
E.L. Cardell ◽  
R.L. Drake ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Endoplasmic Reticulum ◽  
Enzyme Activity ◽  
Malic Enzyme ◽  
Smooth Endoplasmic Reticulum ◽  
Rat Hepatocytes ◽  
Mature Male ◽  
Treatment Groups ◽  
Malic Enzyme Activity ◽  
Biochemical Analyses

Thirty-three mature male Holtzman rats were randomly placed in 3 treatment groups: Controls (C); Ethanolics (E); and Wine drinkers (W). The animals were fed synthetic diets (Lieber type) with ethanol or wine substituted isocalorically for carbohydrates in the diet of E and W groups, respectively. W received a volume of wine which provided the same gram quantity of alcohol consumed by E. The animals were sacrificed by decapitation after 6 weeks and the livers processed for quantitative triglycerides (T3), proteins, malic enzyme activity (MEA), light microscopy (LM) and electron microscopy (EM). Morphometric analysis of randomly selected LM and EM micrographs was performed to determine organellar changes in centrilobular (CV) and periportal (PV) regions of the liver. This analysis (Table 1) showed that hepatocytes from E were larger than those in C and W groups. Smooth endoplasmic reticulum decreased in E and increased in W compared to C values.

SEM Evaluation of Fluoride Pretreatment Effects on Acid-Etching of Caries-Like Enamel Lesions In Vitro

1981 ◽  
Vol 39 ◽  
pp. 474-475
Author(s):  
M. John Hicks
Keyword(s):  
Dental Enamel ◽  
Preventive Measure ◽  
Acid Etching ◽  
Distilled Water ◽  
Adhesive Resin ◽  
Fluoride Treatment ◽  
Treatment Groups ◽  
Pretreatment Effects ◽  
Preparation Techniques

Acid-etching of enamel surfaces has been performed routinely to bond adhesive resin materials to sound dental enamel as a caries-preventive measure. The effect of fluoride pretreatment on acid-etching of enamel has been reported to produce inconsistent and unsatisfactory etching patterns. The failure to obtain an adequate etch has been postulated to be due to fluoride precipitation products deposited on the enamel surface. The purpose of this study was to evaluate the effects of fluoride pretreatment on acid-etching of carieslike lesions of human dental enamel.Caries-like lesions of enamel were created in vitro on human molar and premolar teeth. The teeth were divided into two fluoride treatment groups. The specimens were exposed for 4 minutes to either a 2% Sodium Fluoride (NaF) solution or a 10% Stannous Fluoride (SnF2) solution. The specimens were then washed in deionized-distilled water. Each tooth was sectioned into four test regions. This was carried out to compare the effects of various time exposures (0 to 2 minutes) and differing concentrations (10 to 60% w/w) of phosphoric acid (H3PO4) on etching of caries-like lesions. Standard preparation techniques for SEM were performed on the specimens.

Evaluation of hippocampus in rhesus monkeys after chronic (1-year) inhalation exposure to marijuana: I. Electron microscopy

1990 ◽  
Vol 48 (3) ◽  
pp. 398-399
Author(s):  
A.M. Andrews ◽  
S.W. Wilson ◽  
A.C. Scallet ◽  
S.F. Ali ◽  
J. Bailey ◽  
...  
Keyword(s):  
Synaptic Vesicles ◽  
Rhesus Monkeys ◽  
Low Dose ◽  
Inhalation Exposure ◽  
Synaptic Cleft ◽  
High Dose ◽  
Withdrawal Time ◽  
Treatment Groups ◽  
Ultrastructural Evidence ◽  
Male Rhesus

Exposure of rhesus monkeys (Macaca mulatta) to marijuana via inhalation or to intravenous delta-9-tetrahydrocannabinol (THC), reportedly caused ultrastructural evidence of increased synaptic width. Chronic marijuana smoke in a single rhesus monkey examined after a six month withdrawal time caused ultrastructure changes in the septal, hippocampal and amygdala regions; the synaptic cleft was widened, electron opaque material was found in the cleft and in the pre- and postsynaptic regions, with some clumping of the synaptic vesicles. The objective of our study was to assess neuropathological alterations produced by chronic inhalation of marijuana smoke.Nineteen male rhesus monkeys, 3-5 years of age and weighing 3-8 kg, were divided into four treatment groups: a) sham control, b) placebo smoke (7 days/ week) c) low dose marijuana (2 times/week with 5 days/week sham) and d) high dose marijuana (7 times/week). A smoke exposure consisted of smoke from one cigarette (2.6% THC) burned down to 10 mm butt length. Smoke was administered via smoke generator (ADL II, Arthur D. Little, Inc. Cambridge, MA) and nose-mouth only masks (local production) equipped with one-way valves.

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