scholarly journals Physiology of Pea Fruits I. the Developing Fruit

1955 ◽  
Vol 8 (2) ◽  
pp. 137 ◽  
Author(s):  
HS Mckee ◽  
RN Robertson ◽  
JB Lee
Keyword(s):  
Ascorbic Acid ◽  
Cell Volume ◽  
Starch Content ◽  
Dry Weight ◽  
Metabolic Changes ◽  
Soluble Carbohydrate ◽  
Soluble Nitrogen ◽  
Fresh Weight ◽  
Protein Nitrogen ◽  
Respiration Rates

Pea fruits from two crops were sampled at different times from flowering. Changes in the fresh weight, dry weight, starch, soluble carbohydrate, protein nitrogen, and soluble nitrogen in both seeds and hulls were followed in two seasons and related Jo the changes in cell volume in the seeds. In one season respiration rates and phosphate, pectin, and ascorbic acid contents were also investigated. The seeds gained more carbohydrate and nitrogen than was lost by the hulls. Starch and protein were synthesized rapidly by the seeds. The increase in starch content in the seeds was followed by a decrease in soluble carbohydrate content, after which the seed ceased to accumulate water. These metabolic changes are discussed in the light of recent biochemical knowledge, and in relation to more detailed biochemical investigations in progress.

scholarly journals Starch Synthesis and Changes in Uridine Diphosphorlase in the Developing Wheat Grain

1969 ◽  
Vol 22 (6) ◽  
pp. 1321 ◽  
Author(s):  
JF Turner
Keyword(s):  
Reducing Sugars ◽  
Starch Synthesis ◽  
Dry Weight ◽  
Soluble Nitrogen ◽  
Wheat Grain ◽  
Fresh Weight ◽  
Sharp Rise ◽  
Protein Nitrogen ◽  
Starch Formation ◽  
Main Substrate

The enzymes UDPG pyrophosphorylase and ADPG pyrophosphorylase were assayed during the growth of wheat grains. Changes in fresh weight, dry weight, water, sucrose, reducing sugars, starch, total nitrogen, protein nitrogen, and soluble nitrogen were followed simultaneously. Throughout development the activity of UDPG pyrophosphorylase per grain was much greater than the activity of ADPG pyrophosphorylase. Both enzymes increased in activity during the phase of starch synthesis and a sharp rise in ADPG pyrophosphorylase was associated with the onset of rapid starch formation. ADPG pyrophosphorylase activity decreased to a very low level when starch formation in the grain ceased. UDPG pyrophosphorylase activity also decreased at this time. Although the participation of UDPG is not excluded, it is suggested that the main substrate for starch synthesis in the wheat grain is ADPG. A mechanism for the synthesis of starch from sucrose is proposed.

Relationship Between Grain Nitrogen, Non-Protein Nitrogen and Nucleic Acids During Wheat Grain Development

1979 ◽  
Vol 6 (4) ◽  
pp. 449 ◽  
Author(s):  
GR Donovan
Keyword(s):  
Total Nitrogen ◽  
Culture Medium ◽  
Nitrogen Concentration ◽  
Dry Weight ◽  
Grain Development ◽  
Fresh Weight ◽  
Wheat Varieties ◽  
Protein Nitrogen ◽  
Apparent Relationship ◽  
Grain Nitrogen

Eight wheat varieties which normally produce grain of different final percentage nitrogen content were grown under field and glasshouse conditions. The final percentage grain nitrogen of the field grown varieties ranked in the expected order; however, total nitrogen/grain, DNAIgrain, RNA/grain and non-protein nitrogen/grain during grain development differed between varieties. DNA/grain reached a maximum value in all varieties between 21 and 28 days post- anthesis, suggesting a longer period of cell division than previously reported. There was no apparent relationship between final percentage grain nitrogen and either DNA, total grain RNA or the concentration of grain amino acids during development. Heads from glasshouse grown wheat were detached at 8 days postanthesis and grown in liquid culture under conditions where the nitrogen concentration of the culture medium was varied. Fresh weight/grain, DNA/grain, RNA/grain and total grain nitrogen all increased with increasing nitrogen concentration in the culture medium, but grain dry weight remained constant at the different nitrogen concentrations. The changes in fresh weight/grain, DNA/grain and RNA/grain were not the same for all varieties. A possible relationship between total grain nitrogen and DNA/grain and RNA/grain during seed development exists for heads grown in culture for individual varieties. This apparent relationship for individual varieties cannot be used to explain intervarietal differences in total grain nitrogen because in some cases different varieties grown under identical culture conditions, although producing grain of equivalent total nitrogen, had widely differing levels of both DNA and RNA per grain.

Effects of nitrogen fertilizers on total nitrogen, soluble nitrogen and soluble carbohydrate contents of grass

1962 ◽  
Vol 59 (3) ◽  
pp. 387-392 ◽  
Author(s):  
T. Z. Nowakowski
Keyword(s):  
Ammonium Sulphate ◽  
Total Nitrogen ◽  
Sodium Nitrate ◽  
Nitrogen Fertilizers ◽  
Soluble Carbohydrates ◽  
Soluble Carbohydrate ◽  
Soluble Nitrogen ◽  
Protein Nitrogen ◽  
Total N ◽  
Nitrate N

Italian rye-grass given ammonium sulphate or sodium nitrate at 56 or 112 lb. N/acre was analysed for total nitrogen, soluble nitrogen (non-protein-nitrogen) and soluble carbohydrates.Ten days after applying fertilizer the differences in total-N between the grass receiving 56 and grass receiving 112 lb. N/acre were very small. Total-N in grass decreased with growth, but the effect of the rate of nitrogen on total-N increased. At first the grass given sodium nitrate contained more soluble nitrogen than grass given ammonium sulphate, the difference being greater at 56 lb. N/acre; soluble nitrogen decreased with increasing growth. Ten days after applying fertilizer, the nitrate-N content of grass was very high (ranging from 0·1 to 0·9% in the D.M.) and it gradually decreased. At both levels of nitrogen application, grass given sodium nitrate contained much more nitrate-N than grass given ammonium sulphate. Forty days after applying nitrogen the nitrate-N contents of grass which received 56 and 112 1b. N/acre as ammonium sulphate were 0·039 and 0·222% of the dry matter, respectively; the grass supplied with sodium nitrate gave values of 0·082 and 0·438%.Total soluble carbohydrates in the grass were small early in growth and gradually increased. Nitrogen dressings had little effect on the content of soluble sugars (glucose + fructose + sucrose) but greatly decreased the fructosan. The pattern of changes in the total soluble carbohydrate content followed that in fructosan content. Early in growth, the total soluble carbohydrate/crude protein ratio was very small in grass from all treatments except the ‘control’. This ratio increased with growth and at the last sampling was 2·13 in grass receiving no nitrogen, and in grass supplied with 56 and 112 lb. N/acre as ammonium sulphate it was 1·44 and 0·72 respectively; the corresponding figures for grass receiving sodium nitrate were 1·13 and 0·66. The total soluble carbohydrate carbon/soluble nitrogen ratio in grass with no nitrogen was 18 at the first sampling and it increased gradually, reaching 70 at the last sampling. This ratio was considerably less with all nitrogen treatments than with ‘control’. The values obtained with 112 lb. N/acre were less than those obtained with 561b./acre, irrespective of the form of nitrogen used.The relationship between the soluble carbohydrate carbon content and the soluble nitrogen in grass is illustrated graphically and discussed.

scholarly journals Effect of ascorbic and salicylic acids on growth and flowering of Gazania cv. Frosty Kiss Mixed

2020 ◽  
Vol 26 (4) ◽  
pp. 537-544
Author(s):  
Abdul Kareem A.J. Mohammad Saeed
Keyword(s):  
Ascorbic Acid ◽  
Leaf Area ◽  
Growth Traits ◽  
Foliar Spray ◽  
Dry Weight ◽  
Fresh Weight ◽  
Flowering Duration ◽  
Flowering Date ◽  
Flower Diameter ◽  
Number Of Leaves

Abstract An experiment was conducted during the season 2018-2019 in the lath house to study the effect of foliar spray with Ascorbic Acid (AsA) and Salicylic Acid (SA) on vegetative and flowering growth traits of Gazania (Gazania rigens L.) cv. Frosty Kiss Mixed. The experiment was designed as a factorial experiment (3×3) in Randomized Complete Blocks Design (RCBD) with three replicates. Plants were sprayed with concentrations of 0, 100, and 200 mg L-1 of AsA, and 0, 150 and 300 mg L-1 of SA. Foliar spray with 200 mg L-1 of AsA was superior on increasing number of leaves (40.00), number of suckers (9.22), leaf area (2174.09 cm2), leaves dry weight (6.36 g), number of flowers (5.64), early of flowering date (97.22 days), flower fresh and dry weight (4.78 g) (0.51 g) respectively, while spray with a concentration of 100 mg L-1 led to increase flower diameter (6.37 cm) and flowering duration (10.85 days). Foliar spray with SA improved all vegetative and flowering traits. Treatment of 300 mg L-1 was the most significant effect in increasing number of leaves (39.56), leaf area (2182.06 cm2), leaves fresh weight (43.93 g), number of flowers (5.83), flower diameter (6.31 cm), early of flowering date (98.07 days), and flower dry weight (0.50 g). The interaction effect between the studied factors was significant in all studied traits. Treatment of AsA200×SA150 was the most effective and gave the best results in terms of number of suckers (9.11), dry weight of leaves (6.49 g), number of flowers (5.87), flower diameter (6.44 cm), flowering date (96.98 days), flowering duration (10.89 days).

scholarly journals Characteristic of the nutritive value of the protein from rye caruopses. I. Amino acid composition of protein and the nitrogen forms in the caryopses of ten rue varieties from the breeding collection

2015 ◽  
Vol 27 (1) ◽  
pp. 105-114 ◽  
Author(s):  
R. Kubiczek ◽  
M. Rakowska
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Composition ◽  
Protein Content ◽  
Acid Composition ◽  
Nutritive Value ◽  
Dry Weight ◽  
High Protein ◽  
Soluble Nitrogen ◽  
Protein Nitrogen

Total and soluble nitrogen, protein and non-protein -nitrogen was determined as well as the amino acid composition of the caryopses of ten rye varieties including three bred in Poland and cultivated on a commercial scale: 'Dańkowskie Złote', 'Dańkowskie Selekcyjne' and 'Borkowskie Tetra'. and seven foreign varieties characterized by a high total protein content (11.9-16.4% in dry weight). In the varieties examined the amount of protein nitrogen increased in the same degree as did the content of total nitrogen. The amino acids limiting the nutritive value of the protein in rye caryopses were mostly lysine and methionine, and in the varieties with high protein content tryptophan. The low-protein varieties had a relatively higher content of lysine, sulphur amino acids, tryptophan and other amino acids (as % of protein) than the high protein ones, but their absolute amino acid content (as % of dry weight) was lower.

scholarly journals (447) Response of Two Salt-stressed Cultivars of Sweetpotato to Changes in Calcium Levels under In Vitro Conditions

HortScience ◽  
2005 ◽  
Vol 40 (4) ◽  
pp. 1035A-1035
Author(s):  
Devi Prasad V. Potluri ◽  
Nkechiyere Nwami
Keyword(s):  
Salt Stress ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Metabolic Changes ◽  
Soluble Carbohydrate ◽  
Ms Medium ◽  
Shoot Height ◽  
Weight Number ◽  
Adverse Affects

Two cultivars of sweetpotato, `Commensal' and `Salyboro', were subjected to salt stress using axillary bud cultures. The salt levels ranged from 0 to 10 g·L-1. After the initial experiments, levels of calcium in the medium were changed from 3 mm in the MS medium to 1.5, 6, and 12 mm. After 10 weeks of growth, plantlet shoot height, dry weight, number of nodes, levels of proline, soluble carbohydrate, and protein were measured. `Commensal' was tolerant to salt levels up to 4 g·L-1, but `Salyboro' was sensitive to concentrations of salt even at lower concentrations as evidenced by the growth and dry weight. Proline accumulation was higher in the shoot than in the root. The protein: carbohydrate ratios did not change much in `Commensal', but levels of carbohydrates increased in `Salyboro'. Reduction in calcium levels had a synergistic affect on salt-stressed cultivars. Enhanced levels of calcium reduced the inhibitory affects of salt stress. This was more pronounced in `Salyboro', which was susceptible. Proline levels were higher in plants subjected to salt stress and higher levels of calcium than controls, but lower than the plants subjected to salt stress. These and other metabolic changes suggest that calcium can reduce the adverse affects of salt stress in these two sweetpotato cultivars.

Nitrogen metabolism of Picea glauca. III. Diurnal changes of amino acids, amides, protein, and chlorophyll in leaves of expanding buds

1968 ◽  
Vol 46 (7) ◽  
pp. 929-937 ◽  
Author(s):  
D. J. Durzan
Keyword(s):  
Amino Acids ◽  
Picea Glauca ◽  
Moisture Stress ◽  
Rapid Expansion ◽  
Soluble Nitrogen ◽  
Diurnal Changes ◽  
Fresh Weight ◽  
Protein Nitrogen ◽  
Fresh Weight Basis ◽  
Diurnal Patterns

In spring, buds of spruce shoots under moisture stress contracted during the day and expanded at night. Increased water supply to roots removed the shrinkage of buds and stimulated rapid expansion. In leaves of buds about to break and under moisture stress, the alcohol-soluble nitrogen, high in arginine content, was maximal at midday, after midnight, and again at noon the next day. As a percentage of the soluble nitrogen, all compounds, except arginine, contributed most at sunset and again at sunrise. Other prominent amino acids included proline, alanine, γ-aminobutyric acid, and the amides glutamine and asparagine. Except for the amides, these and other compounds, present at lower levels, gave diurnal patterns similar on a fresh weight basis to arginine. Values for protein nitrogen were greatest at 2 p.m. followed by an increase of asparagine nitrogen, and again at 4 a.m., followed by an increase of glutamine nitrogen. At night the composition of newly synthesized protein increased significantly in aspartic acid, serine, glycine, alanine, leucine, and isoleucine. Chlorophyll a increased from 2 p.m. to 10 p.m., whereas chlorophyll b was present at lower levels and showed little diurnal variation.

scholarly journals Interactive Short-term Effects of Waterlogging and Salinity Stress on Growth and Carbohydrate, Lipid Peroxidation, and Nutrients in Two Perennial Ryegrass Cultivars

2017 ◽  
Vol 142 (2) ◽  
pp. 110-118 ◽  
Author(s):  
Xiujie Yin ◽  
Chao Zhang ◽  
Xin Song ◽  
Yiwei Jiang
Keyword(s):  
Lipid Peroxidation ◽  
Salinity Stress ◽  
Perennial Ryegrass ◽  
Dry Weight ◽  
Nutrient Content ◽  
Water Soluble ◽  
Soluble Carbohydrate ◽  
Fresh Weight ◽  
Short Term ◽  
Water Soluble Carbohydrate

Waterlogging can occur in salt-affected turfgrass sites. The objective of this study was to characterize growth and carbohydrate, lipid peroxidation, and nutrient levels in the leaves and roots of two perennial ryegrass (Lolium perenne) cultivars (Catalina and Inspire) to short-term simultaneous waterlogging and salinity stress. Previous research showed that ‘Catalina’ was relatively more tolerant to salinity but less tolerant to submergence than ‘Inspire’. Both cultivars were subjected to 3 and 7 days of waterlogging (W), salinity [S (300 mm NaCl)], and a combination of the two stresses (WS). Across the two cultivars, W alone had little effect on the plants, while both S and WS alone significantly decreased plant height (HT), leaf fresh weight (LFW), leaf dry weight (LDW), root fresh weight (RFW), root dry weight (RDW), leaf nitrogen (LN) and carbon (LC), and leaf and root K+ (RK+), and increased leaf water-soluble carbohydrate (LWSC) and root water-soluble carbohydrate (RWSC), malondialdehyde (MDA), and Na+ content, compared with the control. A decline in chlorophyll content (Chl) was found only at 7 days of WS. Leaf phosphorus (LP) content either decreased or remained unchanged but root phosphorus content increased under S and WS. Reductions in LFW and LDW were found at 3 days of S and WS, whereas RFW and RDW were unaffected until 7 days of S or WS. Both cultivars responded similarly to W, S, and WS with a few exceptions on RDW, LWSC, leaf MDA (LMDA), and root MDA (RMDA). Although WS caused declines in Chl and resulted in higher leaf Na+ (LNa+) and root Na+ (RNa+) than S at 7 days of treatment, S and WS had similar effects on growth, carbohydrate, MDA, N, C, and phosphorus, and K+ content across the two cultivars. The results suggested that S alone largely accounted for the negative effects of WS on plant growth and physiology including alteration of carbohydrate and nutrient content as well as induction of lipid peroxidation.

METABOLIC CHANGES IN DETACHED WHEAT LEAVES FLOATED ON BENZIMIDAZOLE AND THE EFFECT OF THESE CHANGES ON RUST REACTION

1958 ◽  
Vol 36 (5) ◽  
pp. 591-601 ◽  
Author(s):  
D. J. Samborski ◽  
F. R. Forsyth ◽  
Clayton Person
Keyword(s):  
Stem Rust ◽  
Metabolic Changes ◽  
Soluble Carbohydrate ◽  
Soluble Nitrogen ◽  
Progressive Decrease ◽  
Detached Leaves ◽  
Endogenous Substrate ◽  
Wheat Leaves

Stem-rust-infected leaves of resistant (var. Khapli) and susceptible (var. Little Club) wheats were detached and floated on water and benzimidazole, with and without glucose. Khapli leaves were susceptible on water, resistant on 40 p.p.m. benzimidazole, susceptible on 40 p.p.m. benzimidazole with 1% glucose, and resistant on 60 p.p.m. benzimidazole with 1% glucose. Little Club leaves were susceptible in all treatments. Khapli leaves subjected to treatments that increase the endogenous substrate levels, such as floating on water for 2 to 4 days or spraying with DDT 2 or 3 days before detachment, were susceptible on 40 p.p.m. benzimidazole and resistant or partially resistant at higher concentrations.The progressive decrease in protein of detached leaves on water was interrupted on transfer to benzimidazole, after which some resynthesis of protein took place with a corresponding decrease in the soluble nitrogen fraction. Detached leaves on benzimidazole with 1% glucose had more soluble nitrogen than leaves on benzimidazole alone. There was more alcohol-soluble carbohydrate in leaves floated on water than in leaves on benzimidazole.

Physicochemical and nutritional evaluation of Spanish melon landraces

2015 ◽  
Vol 15 (2) ◽  
pp. 177-186 ◽  
Author(s):  
Sandra Escribano ◽  
Almudena Lázaro
Keyword(s):  
Ascorbic Acid ◽  
Genetic Material ◽  
Dry Weight ◽  
Physicochemical Characteristics ◽  
Soluble Solids ◽  
Fresh Weight ◽  
Repetition Effects ◽  
Nutrient Contents ◽  
Solids Content ◽  
The 19Th Century

The study of unknown and therefore unexploited genetic material from landraces and wild relatives could be essential to help modern plant breeders to respond to ongoing requirements and new challenges in food production. The present study evaluates the most relevant physicochemical values and nutrient contents of a genetically unique array of traditional melon varieties, cultivated in Spain at least since the 19th century, and compares them with modern melon hybrids available on the market. This research is complemented with an assessment of variety, environment and repetition effects on each trait to determine their stability. Spanish melon landraces displayed extraordinary diversity with respect to juiciness (70.59–95.97 g/100 g water fresh weight), firmness (20.75–149.89 N), soluble solids content (9.57–16.53 °Brix), pH (5.04–6.38), total sugars (360.21–877.36 mg/g dry weight), carotenoids (0.01–2.05 μg/g fresh weight) and ascorbic acid values (7.55–44.33 mg/100 g fresh weight). A subset of these landraces, belonging to Piel de Sapo and Rochet market classes, revealed remarkably superior values of ascorbic acid in comparison with all commercial varieties, doubling ascorbic acid values with respect to their corresponding market class. Furthermore, most of these landraces exhibited high acidity and accumulated high levels of sugars, fulfilling those sensory and physicochemical characteristics that researchers and breeders have spent many years seeking. The possibilities of these landraces to be used in improvement projects are innumerable; they should be surely taken into account in the near future.

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