The effect of species of grass, stage of maturity and level of intake on the degradation of protein and organic matter in the rumen of steers

1990 ◽  
Vol 41 (4) ◽  
pp. 791 ◽  
Author(s):  
FJ Hart ◽  
J Leibholz
Keyword(s):  
Organic Matter ◽  
Feed Intake ◽  
Retention Time ◽  
Avena Sativa ◽  
Nitrogen Flow ◽  
Paspalum Dilatatum ◽  
Microbial Nitrogen ◽  
Late Maturity ◽  
Grass Stage ◽  
Two Stages

Six Friesian steers (300 kg liveweight) were prepared with rumen and abomasal cannulae, and fed three artificially dried grasses at three levels of organic matter (OM) intake and at two stages of maturity. The grasses were paspalum (Paspalum dilatatum), oats (Avena sativa) and kikuyu (Pennisetum clandestinum). The apparent digestion of organic matter (OM) in the whole tract decreased with increasing maturity for all grasses. The proportion of OM digested in the rumen was greater for paspalum and kikuyu than oats, and increased with advancing maturity for paspalum and kikuyu. Microbial nitrogen flow from the rumen was 26 and 25 g/kg of OM apparently digested in the rumen for paspalum, 32 and 36 for oats and 25 and 14 for the early and late maturity kikuyu. The true degradation of grass protein in the rumen was between 0.70 and 0.80 for all three grasses, at all three feed intakes and for both stages of maturity of the grasses. Total rumen pool size was less for oats than paspalum or kikuyu. The retention time of particles and fluid in the rumen increased with decreasing feed intake for oats.

The development of ruminant digestion in the calf. II.* The digestion of barley and urea

1978 ◽  
Vol 29 (6) ◽  
pp. 1293 ◽  
Author(s):  
J Leibholz
Keyword(s):  
Amino Acids ◽  
Small Intestine ◽  
Feed Intake ◽  
Terminal Ileum ◽  
Dry Matter ◽  
Nitrogen Flow ◽  
Apparent Absorption ◽  
Microbial Nitrogen ◽  
Nitrogen Intake ◽  
Wheat Chaff

Two Friesian calves were fitted with re-entrant cannulae in the duodenum and four Friesian calves were fitted with re-entrant cannulae in the duodenum and terminal ileum within 1 week of birth. At 35 days of age the calves were weaned from milk and offered a diet containing 72% barley, 3.5% urea and 15 % wheat chaff. Total collections of digesta flowing from the duodenum and ileum were made for 12 hr at weekly intervals for 6 weeks after weaning. Of the dry matter ingested, 26% was apparently digested in the stomach 1 week after weaning, 40% by 2 weeks after weaning and increasing to 51% by 6 weeks after weaning. The flow of nitrogen to the duodenum was greater than the nitrogen intake in the first week after weaning, and it gradually decreased to 83% of the nitrogen intake by 6 weeks after weaning. During this period the contribution of microbial nitrogen to the nitrogen flow increased from 28 to 71%. The apparent absorption of amino acids from the small intestine increased with increasing feed intake, but the uptake of methionine was less than the methionine requirement for growth. _________________ * Part I, Aust. J. Agric. Res., 26: 1081 (1975).

scholarly journals Influence of Residual Feed Intake and Cow Age on Dry Matter Intake Post-Weaning and Peak Lactation of Black Angus Cows

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1822
Author(s):  
Cory T. Parsons ◽  
Julia M. Dafoe ◽  
Samuel A. Wyffels ◽  
Timothy DelCurto ◽  
Darrin L. Boss
Keyword(s):  
Milk Production ◽  
Feed Intake ◽  
Dry Matter ◽  
Free Choice ◽  
Residual Feed Intake ◽  
Beef Cows ◽  
Age Effect ◽  
Dry Matter Intake ◽  
Intake Rate ◽  
Two Stages

We evaluated heifer post-weaning residual feed intake (RFI) classification and cow age on dry matter intake (DMI) at two stages of production. Fifty-nine non-lactating, pregnant, (Study 1) and fifty-four lactating, non-pregnant (Study 2) commercial black Angus beef cows were grouped by age and RFI. Free-choice, hay pellets were fed in a GrowSafe feeding system. In Study 1, cow DMI (kg/d) and intake rate (g/min) displayed a cow age effect (p < 0.01) with an increase in DMI and intake rate with increasing cow age. In Study 2, cow DMI (kg/d) and intake rate (g/min) displayed a cow age effect (p < 0.02) with an increase in DMI and intake rate with increasing cow age. Milk production displayed a cow age × RFI interaction (p < 0.01) where both 5–6-year-old and 8–9-year-old low RFI cows produced more milk than high RFI cows. For both studies, intake and intake behavior were not influenced by RFI (p ≥ 0.16) or cow age × RFI interaction (p ≥ 0.21). In summary, heifer’s post-weaning RFI had minimal effects on beef cattle DMI or intake behavior, however, some differences were observed in milk production.

scholarly journals Measurement of microbial nitrogen flow in the duodenum in sheep

1993 ◽  
Vol 42 (2) ◽  
pp. 167-167 ◽  
Author(s):  
WZ Yang ◽  
C. Poncet
Keyword(s):  
Nitrogen Flow ◽  
Microbial Nitrogen

scholarly journals INFLUENCE OF FARMYARD MANURE ON RETENTION AND AVAILABILITY OF NICKEL, ZINC AND LEAD IN METAL-CONTAMINATED CALCAREOUS LOAM SOILS

2017 ◽  
Vol 25 (3) ◽  
pp. 289-296 ◽  
Author(s):  
Muhammad Abbas AZIZ ◽  
Hamaad Raza AHMAD ◽  
Dennis L. CORWIN ◽  
Muhammad SABIR ◽  
Khalid Rehman HAKEEM ◽  
...  
Keyword(s):  
Organic Matter ◽  
Retention Time ◽  
Farmyard Manure ◽  
Calcareous Soils ◽  
Field Capacity ◽  
Soil Samples ◽  
Continuous Irrigation ◽  
Moisture Contents ◽  
Nickel Zinc ◽  
Chloride Salts

Continuous irrigation of soils with untreated effluents can result in the accumulation and translocation of some metals in the soils and plants. Application of farmyard manure (FYM) to such soils may increase or decrease their availability and retention time. Calcareous soils contaminated with 100, 200, and 400mg kg–1 Ni, Zn, and Pb as chloride salts were used, and farmyard manure added (40g kg–1 for 90 days) with moisture contents at field capacity. Soil samples were drawn at 30 day intervals, and metals extracted with (AB-DTPA) C14H23NO3O10. With FYM application of 400 mg kg–1, Ni availability increased from 179 (day 30) to 240 mg kg–1(day 90); Zn from 163 (day 30) to 230 mg kg–1 (day 90), but, Pb decreased from 214 to 161 mg kg–1. FYM forms multi-dentate complex which greatly enhances the Ni and Zn solubility, and organic matter immobilizes Pb in the soil.

Effect of leucaena supplementation level, and provision of urea, on utilisation by sheep of Rhodes grass hay

2001 ◽  
Vol 41 (2) ◽  
pp. 155 ◽  
Author(s):  
I. W. Karda ◽  
G. McL. Dryden
Keyword(s):  
Organic Matter ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rhodes Grass ◽  
Microbial Nitrogen ◽  
Grass Hay ◽  
Digestible Organic Matter ◽  
Ruminal Degradation ◽  
Chloris Gayana ◽  
Dietary Treatments

Tarramba leucaena (Leucaena leucocephala cv. Tarramba) foliage had per kilogram dry matter, 169 g protein and 29.8 g condensed tannins. Its value as a supplement, given either with or without urea, to sheep given a low-quality Callide Rhodes grass (Chloris gayana cv. Callide) hay was studied. Six rumen fistulated sheep (mean s.d. liveweight, 34 1.4 kg) were used to compare 6 dietary treatments in an incomplete latin square design. Rhodes grass hay was given ad libitum either alone, or with urea 7 g/day (U), or with leucaena 150 g/day (L150), or leucaena with urea (L150U), or leucaena 300 g/day (L300), or leucaena with urea (L300U). Digestible organic matter intake was increased significantly by leucaena supplementation although digestibility of the whole diet did not alter. Rumen fluid ammonia-N was not altered by leucaena supplementation, but was increased by urea. This suggests that Tarramba foliage protein has some resistance to ruminal degradation. Liquid and solids passage rates were not affected by the treatments. Microbial nitrogen supply to the intestine (g/day), and the efficiency of microbial nitrogen synthesis (g/kg organic matter apparently digested in the rumen), were increased by leucaena supplementation (P<0.01). Microbial protein synthesis was the only response in which 300 g/day air-dry Tarramba foliage gave improved results over 150 g/day.

The use of cytosine as a marker to estimate microbial protein synthesis in the rumen

1991 ◽  
Vol 1991 ◽  
pp. 107-107
Author(s):  
L.A. Sinclair ◽  
P.C. Garnsworthy ◽  
P.J. Buttery
Keyword(s):  
Protein Synthesis ◽  
Spectrophotometric Analysis ◽  
Diaminopimelic Acid ◽  
Nitrogen Flow ◽  
Microbial Protein ◽  
Hplc Separation ◽  
Microbial Nitrogen ◽  
Purine Content ◽  
Present Trial

Recently methods based upon the HPLC separation and detection of cytosine (Koenig 1980) and the spectrophotometric analysis of the total purine content of microbes (Zinn and Owens 1986) have been proposed to estimate microbial nitrogen flow at the duodenum. Little work has been undertaken in-vivo to evaluate cytosine as a marker and to compare it with current techniques. The present trial was designed to evaluate this marker and to compare it with diaminopimelic acid (DAPA), [3H]leucine and the total purine technique. In addition, recent reports (Hvelpund and Madsen 1985) have indicated that expressing miaobial efficiency in terms of carbohdrate, as opposed to organic matter degraded in the rumen, reduced the variation in microbial yield. This was also investigated.

scholarly journals Relations of Ruminal Fermentation Parameters and Microbial Matters to Odd- and Branched-Chain Fatty Acids in Rumen Fluid of Dairy Cows at Different Milk Stages

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1019 ◽  
Author(s):  
Keyuan Liu ◽  
Yang Li ◽  
Guobin Luo ◽  
Hangshu Xin ◽  
Yonggen Zhang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Nitrogen Flow ◽  
Ruminal Fermentation ◽  
Bacterial Populations ◽  
Microbial Nitrogen ◽  
Branched Chain Fatty Acids ◽  
Branched Chain ◽  
Fermentation Parameters ◽  
Chain Fatty Acids

The purpose of this research was to evaluate whether relationships exist between odd- and branched-chain fatty acids (OBCFAs) originating from milk fat and the corresponding data of ruminal fermentation parameters, microbial populations, and base contents that were used to mark microbial protein in rumen. Nine lactating Holstein dairy cows with similar body weights and parity were selected in this study, and the samples of rumen and milk were collected at the early, middle, and late stages, respectively. The rumen and milk samples were collected over three consecutive days from each cow, and the ruminal and milk OBCFA profiles, ruminal fermentation parameters, bacterial populations, and base contents were measured. The results showed that the concentrations of OBCFAs, with the exception of C11:0 and C15:0, were significantly different between milk and rumen (p < 0.05). The concentrations of anteiso-fatty acids in milk were higher than those in rumen, and the contents of linear odd-chain fatty acids were higher than those of branched-chain fatty acids in both milk and rumen. Significant relationships that existed between the concentrations of C11:0, iso-C15:0, anteiso-C15:0, C15:0, and anteiso-C17:0 in rumen and milk (p < 0.05). The total OBCFA content in milk was positively related to the acetate molar proportion but negatively correlated with isoacid contents (p < 0.05). The populations of Ruminococcus albus, R. flavefacients, and Eubacterium ruminantium were significantly related to milk C13:0 contents (p < 0.05). The adenine/N ratio was negatively related to milk OBCFA content (p < 0.05) but positively associated with the iso-C15:0/iso-C17:0 ratio (p < 0.05). Milk OBCFAs were significantly correlated with ruminal fermentation parameters, ruminal bacterial populations, and base contents. Milk OBCFAs had the potential to predict microbial nitrogen flow, and the prediction equations for ruminal microbial nitrogen flow were established for OBCFAs in dairy milk.

The effect of the pea to wheat ratio and harvest date on the voluntary feed intake,in vivodigestibility and nitrogen retention of pea-wheat bi-crop silages by sheep

2000 ◽  
Vol 2000 ◽  
pp. 81-81 ◽  
Author(s):  
A.T. Adesogan ◽  
M.B. Salawu ◽  
E.R. Deaville
Keyword(s):  
Feed Intake ◽  
Nutritive Value ◽  
Nitrogen Retention ◽  
Harvest Date ◽  
Apparent Digestibility ◽  
N Retention ◽  
Stages Of Growth ◽  
Voluntary Feed Intake ◽  
Two Stages

Pea-wheat bi-crop silages were reported to have moderate nutritive value when the proportion of peas in the sward was less than 200 g/kg (Adesoganet al., 1999). These authors also suggested that improvements in the digestibility, intake and nitrogen (N) balance of the forages may result from increasing the proportion of peas in the sward. This experiment was designed to examine this theory by determining thein vivoapparent digestibility, N retention and voluntary feed intake (VFI) in sheep of pea-wheat bi-crop silages containing different ratios of peas to wheat and harvested at two stages of growth.

The relationship between apparent retention time in the rumen, voluntary intake, and apparent digestibility of legume and grass diets in sheep

1973 ◽  
Vol 24 (6) ◽  
pp. 889 ◽  
Author(s):  
RF Thornton ◽  
DJ Minson
Keyword(s):  
Organic Matter ◽  
Retention Time ◽  
Daily Intake ◽  
Organic Matter Content ◽  
Matter Content ◽  
Apparent Digestibility ◽  
Jaw Movements ◽  
Digestible Organic Matter ◽  
Voluntary Intake ◽  
The Relationship

Eight grasses and six legumes were fed ad libitum at hourly intervals to wethers fitted with large rumen fistulas to determine voluntary intake, apparent digestibility, and the apparent retention time of organic matter in the rumen, as well as the quantity of rumen digesta and the organic matter content of the rumen digesta. The voluntary intake of legumes was 28% higher than that of equally digestible grasses. This difference was caused by a shorter retention time (17%) and a higher amount of organic matter (14%) in the rumen digesta from legume diets than from grass diets. The weight of wet digesta in the rumen of sheep fed on legumes was 7% lower than from those fed on grass. Neither the quantity of digesta nor the quantity of organic matter in the rumen was related to the voluntary intake, digestibility, or retention time of organic matter in the rumen. Number of jaw movements each day and rate of cotton thread digestion were not related to the retention time of the diets either. Daily intake of digestible organic matter (DOMI, g/day) was closely correlated with retention time (RTOM, hr) for the feeds in this study (r = –0.93) and the same relation applied to both temperate and tropical feeds, viz. DOMI = 1276–50.7 RTOM (r=0.96) Differences in voluntary intake between grasses and legumes were attributed to differences in retention time and the density to which the food was packed in the rumen.

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