Milk replacers containing a soybean protein concentrate for preruminant lambs: interrelationships between methionine, cystine and choline

1982 ◽  
Vol 33 (1) ◽  
pp. 117
Author(s):  
NM Malouf ◽  
DM Walker

Preruminant male crossbred lambs, aged 2-5 days at the start of the experiment, were fed on milk replacers containing a soybean protein concentrate (as the sole source of protein) to supply 25 % of the total dietary energy as protein. The minimum intake of methionine plus cystine (M+C) that was coincident with maximum nitrogen balance was estimated (mean�s.e.) at 38.5�1.8 mg/100 kJ diet (equivalent to 3.6�0.2 g sulfur amino acids/16 g nitrogen). When the concentration of M+C was raised to this level by supplementation with DL-methionine, the addition of choline chloride, in amounts to give final concentrations varying from approximately one-seventh up to twice the concentration in cows' milk, did not affect nitrogen balance. The maximum replacement value of cystine for methionine (as judged by the effect on nitrogen balance), in milk replacers adequately supplen~ented with choline chloride and sulfur amino acids, was 47% on a weight basis, or 52% when calculated on the basis of moles of sulfur.

1972 ◽  
Vol 52 (2) ◽  
pp. 321-328 ◽  
Author(s):  
A. D. L. GORRILL ◽  
J. W. G. NICHOLSON ◽  
H. E. POWER

Two experiments were carried out with Holstein heifer calves to determine the effect of supplying a major portion of the protein in milk replacers by isopropanol-extracted protein concentrate from whole herring (FPC) or a mixture of FPC and soybean protein concentrate (SPC). Milk replacers were compared with whole milk in experiment 1. All calves were abruptly weaned when consuming 0.5 kg starter/day, or by 5 weeks of age. Calf performance was similar when the pre-weaning liquid diet was whole milk, or milk replacers containing either milk protein or 50% of the protein from FPC. Average daily gains to weaning and to 26 weeks of age for all calves were 377 and 692 g/day, respectively. Calves in experiment 2 were fed either an all-milk protein milk replacer or one containing 98% of the protein equally from FPC and SPC. Both milk replacers were fed once daily either six or seven times per week. Calf growth to weaning was lower on the FPC–SPC formula (283 vs. 364 g/day, P < 0.01), but was not significantly different (P < 0.05) to 15 weeks of age (618 vs. 643 g/day). Feeding milk replacer six vs. seven times per week had no significant effect on calf growth. Digestibility coefficients of dry matter, nitrogen, and energy were similar in bull calves fed the all-milk or FPC–SPC protein milk replacers, but retention of absorbed nitrogen was less on the latter diet (54 vs. 45%, P < 0.10).


1972 ◽  
Vol 52 (4) ◽  
pp. 665-670 ◽  
Author(s):  
A. D. L. GORRILL ◽  
J. W. G. NICHOLSON

Alkali treatment to disperse a soybean protein concentrate (SPC) was studied in two milk replacer experiments, in which the SPC supplied 50% of the total nitrogen. In experiment 1, six lambs were used in a double 3 × 3 latin square digestion and nitrogen balance trial. The three treatments of the SPC in the milk replacer were: control (no alkali), alkali 1 (0.05 N NaOH at 40 C, neutralized with HCl after 15 min), and alkali 2 (same as 1, but neutralized after 18 hr at 5 C). The remainder of the diet ingredients were homogenized with a Polytron. Alkali treatment increased digestibility of dry matter (91, 93, and 95%, P < 0.01, on the three treatments, respectively), nitrogen, and energy, but tended to reduce the percent of absorbed nitrogen that was retained (63, 61, and 60%, P < 0.20). The control (complete diet prepared with the Polytron) and alkali 1 treatments were compared in a growth and metabolism trial in experiment 2. Growth of a total of 19 lambs from about 4 days to weaning from milk replacer at 26 days of age tended to be lower on the alkali-treated than the control SPC milk replacer (179 vs. 215 g/day, P < 0.20); however, growth to 10 weeks of age was similar for both groups and averaged 250 g/day.


1969 ◽  
Vol 49 (3) ◽  
pp. 315-321 ◽  
Author(s):  
A. D. L. Gorrill ◽  
J. W. G. Nicholson

Milk replacers containing zero (all-milk) or 70% of the total protein from a soybean protein concentrate (soy-milk), with or without methionine supplementation, were fed to Holstein calves. Bull calves digested 91 and 89% of the dry matter (P < 0.05), and 87 and 82% of the nitrogen (P < 0.01) supplied by the all-milk and soy-milk replacers, respectively. Nitrogen retention averaged 41% of that consumed, with no difference due to protein source. DL-methionine (0.1% of the dry replacer) did not increase calf growth or nitrogen retention. Heifer calves fed whole milk, the all-milk replacer or soy-milk replacer plus methionine, and hay and concentrates, gained 496, 550 and 526 g/day, respectively, to weaning at 7 weeks of age (treatment means not significantly different at P < 0.05). Weight gains for these three groups of heifers from 7 to 15 weeks of age were 723, 650 and 599 g/day (significant difference between whole milk and soy-milk at P < 0.05), respectively. It was concluded that the soybean protein concentrate could supply a major portion of the protein in milk replacers for rearing dairy calves.


2021 ◽  
Vol 888 (1) ◽  
pp. 012069
Author(s):  
R T Hertamawati ◽  
Nurkholis ◽  
R Rahmasari

Abstract This study aims to determine the opportunity and characteristics of edamame soybean protein concentrate (eSPC) as a source of amino acid. In this study, edamame soybean protein concentrate (eSPC) was manufactured using various methods of manufacturing solvent-based materials. This experiment consisted of two different methods, namely washing with acid and ethanol solvent. The results of manufacturing eSPC were analyzed for nutrients, especially the protein and amino acids contents. From the presentation and description of data, the best results of edamame soybean processing into protein concentrate (eSPC) was obtained in the acid method with a protein content of 48.83% and a total of 36.66% amino acids. The highest content of lysine was obtained at eSPC processing using 3.77% of acid, while methionine highest was with approximately 1.12%. These results showed that the eSPC is well used as a source of lysine and methionine in poultry feed.


1979 ◽  
Vol 30 (1) ◽  
pp. 125 ◽  
Author(s):  
R Pelaez ◽  
DM Walker

Preruminant male crossbred lambs, aged about 2 weeks at the start of the experiment, were used. Low protein milk replacers (0.10 of total energy as protein) containing isolated soybean protein (ISP-A or ISP-B) as the sole source of protein were supplemented with mixtures of amino acids. Experiment 1a. Six lambs were each given six isonitrogenous diets in a 6 x 6 change-over design with 4-day dietary periods. The diets contained ISP-B and were supplemented with (1) methionine alone, or methionine plus (2) lysine, (3) isoleucine, (4) threonine, (5) valine or (6) tryptophan. Experiment 1b. Groups of six lambs were given diets containing either ISP-A or ISP-B. Each lamb within a group was given six diets in a 6 x 6 change-over design with 4-day dietary periods. One diet was supplemented with six amino acids (Met, Lys, Isol, Thr, Val and Try) whilst the other five diets had one amino acid (not Met) omitted. Nitrogen balances were determined in experiments 1a and 1b on the last 2 days of each dietary period. Experiment 2. Two groups of four lambs were used in a conventional 12-day nitrogen balance experiment. One group was given the milk replacer containing ISP-B supplemented with the six amino acids used in experiment lb, and the other group the same basal diet supplemented only with Met, Lys and Thr. The addition of single amino acids in addition to methionine did not improve nitrogen balances. Lambs given diets supplemented with Met plus Thr, Val or Try had significantly lower nitrogen balances than those given a supplement of Met alone (experiment 1a). Lambs given diets in which Lys or Thr were omitted from the mixture of six amino acids had Lower nitrogen baIances than any other lambs (experiment 1b). It was concluded that Lys and Thr were equally second limiting in ISP-A and ISP-B at the 0.10 protein energy concentration. The retention of apparently digested nitrogen by lambs given ISP-B supplemented with Met, Lys and Thr (0.669) was significantly higher than that of lambs given ISP-B supplemented with six amino acids (0.590; experiment 2). The mean digestibility coefficient of nitrogen in ISP-B (0.842 ± 0.009) was significantly higher than that in ISP-A (0.764 ± 0.014) and the diets containing ISP-B were more readily accepted by the lambs.


2005 ◽  
Vol 88 (4) ◽  
pp. 1217-1222 ◽  
Author(s):  
Wei-Nong Zhang ◽  
Da-Chuan Liu

Abstract A new process for the preparation of soybean protein concentrate (SPC) by directly extracting full-fat soy flour with a mixture of hexane and aqueous ethanol was established. Compared with conventional methods, it has some advantages, such as saving energy and reducing protein denaturation caused by heat action during solvent recovery, because this process saves one step of solvent recovery. The effects of aqueous ethanol concentration and the mixure ratio (hexane to ethanol) on the degree of protein denaturation and product quality were investigated, on the basis of which the orthogonal tests were performed. The optimum technical parameters were obtained by analyzing the results of the orthogonal tests with statistical methods. We found that SPC can be obtained by extracting full-fat soy flour under the following conditions: mixture ratio hexane: 90% ethanol, 9:1, v/v; extraction temperature, 45°C; ratio of solid to solvents, (1:2 w/v); and 5 repeated extractions (15 min each time). The results of quality analysis showed that solubility of the product was improved significantly [nitrogen solubility index (NSI) 46.6%] compared with that for ethanol washing of protein concentrate (NSI 8.7%).


2018 ◽  
Vol 243 ◽  
pp. 448-452 ◽  
Author(s):  
Emiliano M. Ciannamea ◽  
Juan P. Espinosa ◽  
Pablo M. Stefani ◽  
Roxana A. Ruseckaite

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