Relationships between Oesophagostomum columbianum infection and the nutritional status of sheep. III. Serum and tissue protein changes

1969 ◽  
Vol 20 (5) ◽  
pp. 965 ◽  
Author(s):  
RJ Bawden
Keyword(s):  
Single Dose ◽  
Nutritional Status ◽  
Serum Protein ◽  
High Protein ◽  
Pathological Features ◽  
Tissue Protein ◽  
Protein Depletion ◽  
Low Protein ◽  
Oesophagostomum Columbianum ◽  
Protein Group

The infection of young sheep with 1500 infective Oesophagostomum columbianum larvae was associated with the development of hypoproteinaemia resulting basically from a hypoalbuminaemia. Hypogammaglobulinaemia and hyperbetaglobulinaemia also developed in the infected animals. The concentration of α-globulin in the serum was not affected by infection. The extent and duration of the pathological features in the serum protein concentrations varied with the plane of nutrition. More severe effects were noted in sheep which had been maintained on a low protein ration and which exhibited severe protein depletion of the liver, spleen, and thymus, than in animals on a high protein ration. Within the low protein group the most severe depressions in serum protein concentrations resulted from infection with small multiple doses of larvae compared with the same total number given in a single dose.

Relationships between Oesophagostomum columbianum infection and the nutritional status of sheep. II. Haematological changes

1969 ◽  
Vol 20 (3) ◽  
pp. 601 ◽  
Author(s):  
RJ Bawden
Keyword(s):  
Single Dose ◽  
Nutritional Status ◽  
Cell Volume ◽  
Packed Cell Volume ◽  
Haemoglobin Concentration ◽  
High Protein ◽  
Low Protein ◽  
Oesophagostomum Columbianum ◽  
Haematological Changes ◽  
The Mean

Young sheep maintained on a low protein ration showed significant reductions in haemoglobin concentration, packed cell volume, and number of circulating erythrocytes following infection with 1500 Oesophagostomum columbianum larvae. Similar infections of sheep maintained on a high protein ration had no significant effect on the various haematological values. The development of anaemia in the inadequately fed sheep was more severe in those animals which had been infected with five consecutive daily doses of 300 larvae than in those which had received 1500 larvae in a single dose. Little change was apparent throughout the experiment in the values for the mean corpuscular values of volume, haemoglobin, and haemoglobin concentration.

Corrigendum - The endogenous phosphorus excretion of preruminant lambs

1987 ◽  
Vol 38 (6) ◽  
pp. 1061
Author(s):  
DM Walker ◽  
SJ Al-Ali
Keyword(s):  
Daily Intake ◽  
Experimental Period ◽  
Sole Source ◽  
High Protein ◽  
Low Protein ◽  
Bone Ash ◽  
Protein Group ◽  
Gross Energy ◽  
Protein Milk ◽  
Milk Replacers

Twenty-seven preruminant male crossbred lambs, aged 1-2 days at the start of the experiment, were used. Three lambs were sacrificed on day 1 and selected bones taken for analysis. Twenty-four lambs, in groups of three, were bottle-fed on low-phosphorus milk replacers in a 2 x 4 factorial experiment. The sole source of protein in the diets was whole beef blood, supplemented with L-isoleucine and DL-methionine, to supply either 10 or 25% of the total dietary energy as protein. At each protein level the diets were supplemented with CaCO3 to provide four ratios of Ca:P (0.5:1, 1:1, 2:1 and 10:1). The daily intake of gross energy was controlled at 880 kJ-0.73. Faeces and urine were collected separately each day and bulked during the last seven days of the experiment for the estimation of N, Ca and P. Serum Ca and P concentrations were determined at weekly intervals. The lambs were sacrificed at the end of the experimental period of 21 days and selected bones were taken for analysis. The concentrations of bone ash, Ca and P all decreased significantly when compared with values determined at the start of the experiment. The lambs given the high-protein diets showed significantly greater losses of bone ash, Ca and P, and had lower serum P, but higher serum Ca concentrations, than lambs fed on the low-protein milk replacers, irrespective of the dietary Ca:P ratios. Phosphorus excretions during the last seven days of the experiment were as follows: faecal P (day-1); low-protein group (n = 12), 3.1 � 0.5 mg kg-1; high-protein group (n = 11) , 4.1 � 0.5 mg kg-1; all lambs (n = 23), 3.6 � 0.3 mg kg-1. Urinary P (day-1): low-protein group (n = 12), 0.92 � 0.22 mg kg-1; high-protein group (n = 11) , 0.61 � 0.03 mg kg-1; all lambs (n = 23), 0.77 � 0.12 mg kg-1. Faecal and urinary P excretion was unaffected by variation in the dietary Ca:P ratio.

Effects of feeding varying levels of protein and phosphorus in relation to bone disorders in cattle

1975 ◽  
Vol 15 (74) ◽  
pp. 321 ◽  
Author(s):  
BD Siebert ◽  
DMR Newman ◽  
B Hart ◽  
GL Michell
Keyword(s):  
High Protein ◽  
Blood Levels ◽  
Fresh Weight ◽  
Treatment Groups ◽  
Low Protein ◽  
Metacarpal Bones ◽  
Protein Group ◽  
Calcium Phosphorus ◽  
Calcium And Phosphorus ◽  
Total Fresh Weight

Steers were fed low or high protein diets with varying levels of phosphorus. Two steers on the low protein diet developed a disorder which clinically resembled osteomalacia after 21 weeks of experimental feeding, and other animals on the same diet showed varying degrees of lameness. The total fresh weight, total mineral content, and the calcium and phosphorus levels of the metacarpal bones were significantly lower in the low protein group. Total fresh weight, total ash content of the rib bones was also lower although there were no differences between groups in the calcium and phosphorus content of rib bones. There were significant differences between treatment groups in blood levels of calcium, phosphorus and protein. The carcase weight of high protein animals was significantly greater than that of the low protein animals. Phosphorus level in the diet had little effect on any tissue measurement. The results showed that a bone disorder could develop in animals fed adequate phosphorus but inadequate protein. The implications of the experiment are considered in relation to the levels of protein and phosphorus in pasture throughout northern Australia.

Corrigendum to: IGF-I is not a useful marker for nutritional status in the growing pig under commercial conditions

2001 ◽  
Vol 52 (7) ◽  
pp. 791
Author(s):  
L. Ma ◽  
F. R. Dunshea ◽  
Y. M. Brockwell ◽  
R. L. Inglis ◽  
D. J. Kingston ◽  
...  
Keyword(s):  
Weight Gain ◽  
Nutritional Status ◽  
Dietary Protein ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein ◽  
Igf I ◽  
Protein Diets

Plasma hormone concentrations were measured in gilts after fasting, long-term protein restriction, or supplementation. In 11-week-old pigs fasted overnight, plasma insulin, glucagon, gastrin, urea, and glucose were increased 30 min after re-feeding (P < 0.05), whereas IGF-I did not change. In 16-week-old gilts fed a standard commercial diet [14.6% crude protein (CP)], or a high-protein diet (16.7% CP) for 4 weeks, the high-protein diet increased weight gain (13%; P < 0.05) and carcass weight (4%; P < 0.05), but did not alter plasma IGF-I, insulin, or glucagon. In 10-week-old gilts fed high-protein diets (19.4% and 18.3% CP), or low-protein diets (15.5% and 13.3% CP) for 12 weeks during the grower and finisher phases, respectively, the low-protein diet decreased weight gain (18%; P < 0.001) and carcass weight (11%; P < 0.01), with a marked increase in plasma glucagon (P < 0.05), no change in insulin, and only a trend towards decreased IGF-I (P = 0.1). The pigs were more sensitive to altered dietary protein at 10 weeks of age than at 16 weeks. Plasma IGF-I was not responsive to the short-term effects of feeding or the long-term effects of dietary protein. Glucagon could provide a useful marker for nutritional status in young pigs, provided that time of feeding is taken into account.

INFLUENCE OF PRIOR DIETARY PROTEIN LEVELS ON RESISTANCE TO THE STRESS OF PROTEIN DEPLETION

PEDIATRICS ◽  
1962 ◽  
Vol 29 (1) ◽  
pp. 90-96
Author(s):  
Oliver W. Vaughan ◽  
L. J. Filer ◽  
Helen Churella
Keyword(s):  
Plasma Protein ◽  
Protein Turnover ◽  
High Protein Diet ◽  
High Protein ◽  
Intravenous Injections ◽  
Protein Levels ◽  
Low Protein ◽  
Protein Group ◽  
High Protein Diets ◽  
Protein Diets

One-day-old piglets were fed diets of either 50% or 14% protein for 8 weeks; they were given intravenous injections with S35-methionine-labeled plasma protein and were given a nonprotein diet. The plasma protein turnover and the nitrogen excreted were estimated by measuring the S35 activity and the nitrogen in aliquots of blood, urine and feces. During a 102-day period of protein privation, the animals that had received the high-protein diet lost little weight, while pigs previously fed a low level of protein lost 4.4 kg. However the high-protein group had a considerably faster rate of plasma protein turnover, catabolized a much large quantity of protein, and excreted more S35 and nitrogen than did the low-protein group. It is concluded that high-protein diets may make pigs less well able to cope with the stress of sudden protein deprivation.

IGF-I is not a useful marker for nutritional status in the growing pig under commercial conditions

2001 ◽  
Vol 52 (5) ◽  
pp. 603
Author(s):  
L. Ma ◽  
F. R. Dunshea ◽  
Y. M. Brockwell ◽  
R. L. Inglis ◽  
D. J. Kingston ◽  
...  
Keyword(s):  
Weight Gain ◽  
Nutritional Status ◽  
Dietary Protein ◽  
High Protein Diet ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein ◽  
Igf I ◽  
Protein Diets

Plasma hormone concentrations were measured in gilts after fasting, long-term protein restriction, or supplementation. In 11-week-old pigs fasted overnight, plasma insulin, glucagon, gastrin, urea, and glucose were increased 30 min after re-feeding (P < 0.05), whereas IGF-I did not change. In 16-week-old gilts fed a standard commercial diet [14.6% crude protein (CP)], or a high-protein diet (16.7% CP) for 4 weeks, the high-protein diet increased weight gain (13%; P < 0.05) and carcass weight (4%; P < 0.05), but did not alter plasma IGF-I, insulin, or glucagon. In 10-week-old gilts fed high-protein diets (19.4% and 18.3% CP), or low-protein diets (15.5% and 13.3% CP) for 12 weeks during the grower and finisher phases, respectively, the low-protein diet decreased weight gain (18%; P < 0.001) and carcass weight (11%; P < 0.01), with a marked increase in plasma glucagon (P < 0.05), no change in insulin, and only a trend towards decreased IGF-I (P = 0.1). The pigs were more sensitive to altered dietary protein at 10 weeks of age than at 16 weeks. Plasma IGF-I was not responsive to the short-term effects of feeding or the long-term effects of dietary protein. Glucagon could provide a useful marker for nutritional status in young pigs, provided that time of feeding is taken into account.

Studies on the immunity of sheep to Oesophagostomum columbianum: effects of low-protein diet on resistance to infection and cellular reactions in the gut

Parasitology ◽  
1974 ◽  
Vol 69 (2) ◽  
pp. 239-255 ◽  
Author(s):  
Colin Dobson ◽  
Richard J. Bawden
Keyword(s):  
Mast Cell ◽  
Protective Immunity ◽  
Cellular Proliferation ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Low Protein ◽  
Cell Counts ◽  
Oesophagostomum Columbianum ◽  
Protein Diets

Adult Oesophagostomum columbianum populations were larger in sheep fed low-protein diets than in adequately fed animals. Diet did not influence the numbers of larva which became established in sheep. Sheep fed high-protein diets eliminated more worms and were more immunologically competent than poorly fed animals. More encapsulated larvae, showing arrested development, were found in adequately fed sheep than in those fed low-protein diets. Adult O. columbianum produced eggs at an earlier time after infection in poorly fed sheep than worms in well fed sheep. More eggs/female worm were produced in sheep on a low-protein diet compared with the number of eggs produced in well fed hosts over the last week of the infection. The effects of immunity on the behaviour of the worm in both host diet groups is discussed.There was a greater cellular proliferation in the intestines of infected adequately fed sheep than in infected animals on low-protein diets. These changes were most pronounced in the large intestine where the adult parasites were found. The macrophage-lymphocyte series of cells underwent hyperplasia in well fed animals but these changes were reduced, particularly among the plasma cells, in sheep fed low-protein diets. Increased mucin and mast cell counts were observed in sheep on high-protein, but not in hosts on low-protein diets: the intestinal populations of eosinophil and globule leucocytes were also reduced in poorly fed sheep.The relationship of these various cellular reactions and their effect on the protective immunity of sheep to O. columbianum is discussed. It was concluded that the increased susceptibility of protein deprived sheep to O. columbianum infections was associated with malfunctions of the innate immunity of the gut, involving decreased peristalsis and failure of the mucin cell response, and with reduction of the adaptive immune response which was reflected by impoverished lymphocyte and plasma cell reactions, and possibly with poor cooperation between sensitized lymphocytes, antibodies and the mast cell-granulocyte effector mechanisms in protective immunity.

The establishment and survival of Oesophagostomum columbianum in male and female sheep given high and low protein diets

1969 ◽  
Vol 20 (6) ◽  
pp. 1151 ◽  
Author(s):  
RJ Bawden
Keyword(s):  
Marked Reduction ◽  
High Protein ◽  
Protein Diet ◽  
Low Protein Diet ◽  
Male And Female ◽  
Low Protein ◽  
Oesophagostomum Columbianum ◽  
Protein Diets ◽  
Female Sheep ◽  
Male Sheep

Young sheep fed on a low protein (6%) diet were more susceptible to infection with O. columbianum than those on a high protein (18%) diet. Both the number of adult nematodes recovered 56 days after infection, and the fecundity of the female worms prior to autopsy, were greater in the former group. Examinations at the 10th and 56th days after infection revealed a marked reduction with time in the numbers and extent of distribution of macroscopic nodules, associated with the parasite, throughout the intestines; no differences associated with the diet were recorded. Differences in the susceptibility of the host were also associated with the host's sex. Thus the male sheep generally harboured more adult nematodes than the female sheep at autopsy. This effect was most pronounced with the sheep on the low protein diet. The female nematodes which parasitized the female sheep were more fecund than those in the male sheep.

The influence of dietary protein intake on milk production and blood composition of high-yielding dairy cows

1976 ◽  
Vol 43 (3) ◽  
pp. 357-369 ◽  
Author(s):  
R. J. Treacher ◽  
W. Little ◽  
K. A. Collis ◽  
A. J. Stark
Keyword(s):  
Dairy Cows ◽  
Protein Intake ◽  
Agricultural Research ◽  
Energy Levels ◽  
High Protein ◽  
Agricultural Research Council ◽  
Low Protein ◽  
Significant Difference ◽  
Protein Group ◽  
The Mean

SummaryCows were fed either 75 or 100% of the recommended intake levels for protein and 100% of recommended energy levels (Agricultural Research Council, 1965) from 8 weeks pre-calving until 14 weeks post calving. From 14 weeks post calving and to the end of lactation all the cows received 100% of the recommended protein and energy intakes.The mean of the 305-d milk yields of the 2 groups was not significantly different and although cows on the lower protein intake produced less lactose during the first 14 weeks of lactation there was no significant difference in total lactose, fat, protein or total solids production between the groups. In both groups blood packed-cell volume, red cell count and haemoglobin decreased during the first 10 weeks of lactation and then began to increase in the high-protein group. The cows receiving the low-protein diet showed a similar increase only when they received the high-protein ration from 14 weeks post calving. The mean interval from calving to conception was 27·5 weeks in the high-protein group and 20 weeks in the low-protein group.It is concluded that feeding 75% of protein requirements to dairy cows during the first 14 weeks of lactation does not reduce milk yield or quality significantly and probably has no adverse effect on fertility.

scholarly journals Meeting nutritional targets of critically ill patients by combined enteral and parenteral nutrition: review and rationale for the EFFORTcombo trial

2020 ◽  
Vol 33 (2) ◽  
pp. 312-320
Author(s):  
Aileen Hill ◽  
Daren K. Heyland ◽  
Gunnar Elke ◽  
Stefan J. Schaller ◽  
Reto Stocker ◽  
...  
Keyword(s):  
Parenteral Nutrition ◽  
Critically Ill ◽  
Critically Ill Patients ◽  
Nutrition Therapy ◽  
High Protein ◽  
Nutritional Risk ◽  
International Guidelines ◽  
Low Protein ◽  
Protein Group ◽  
Nutrition Review

AbstractWhile medical nutrition therapy is an essential part of the care for critically ill patients, uncertainty exists about the right form, dosage, timing and route in relation to the phases of critical illness. As enteral nutrition (EN) is often withheld or interrupted during the intensive care unit (ICU) stay, combined EN and parenteral nutrition (PN) may represent an effective and safe option to achieve energy and protein goals as recommended by international guidelines. We hypothesise that critically ill patients at high nutritional risk may benefit from such a combined approach during their stay on the ICU. Therefore, we aim to test if an early combination of EN and high-protein PN (EN+PN) is effective in reaching energy and protein goals in patients at high nutritional risk, while avoiding overfeeding. This approach will be tested in the here-presented EFFORTcombo trial. Nutritionally high-risk ICU patients will be randomised to either high (≥2·2 g/kg per d) or low protein (≤1·2 g/kg per d). In the high protein group, the patients will receive EN+PN; in the low protein group, patients will be given EN alone. EN will be started in accordance with international guidelines in both groups. Efforts will be made to reach nutrition goals within 48–96 h. The efficacy of the proposed nutritional strategy will be tested as an innovative approach by functional outcomes at ICU and hospital discharge, as well as at a 6-month follow-up.

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