scholarly journals Proteolysis of meat and bone meal to increase utilisation

2014 ◽  
Vol 54 (2) ◽  
pp. 200 ◽  
Author(s):  
G. J. Piazza ◽  
R. A. Garcia
Keyword(s):  
Time Course ◽  
Group Formation ◽  
Farm Animals ◽  
Soluble Form ◽  
Meat Industry ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Short Treatment ◽  
Insoluble Material ◽  
Flocculation Activity

Meat and bone meal (MBM), an important by-product of the meat industry, is the ground, rendered remainder of farm animals after removal of the hide and meat. Most protein in MBM is insoluble, which limits its usefulness. Defatted, milled porcine MBM was subjected to saturating amounts of trypsin, a selective protease, and subtilisin, a protease with broad selectivity. Samples were withdrawn over a 48-h time course of hydrolysis and filtered to remove insoluble material. The rate at which the MBM protein was converted to a soluble form was equivalent for both proteases. Over the time course, trypsin generated fewer free amino groups than did subtilisin, and at a specified time, the molecular weight (MW) of the soluble trypsin hydrolysate was higher than that of the subtilisin hydrolysate. Assay of amino group formation showed that the proteases were still active even after soluble protein generation had ceased. The hydrolysates are useful for a variety of food and non-food uses. The hydrolysates were tested for flocculation activity in an ongoing effort to find sources for renewable flocculant. Kaolin flocculant activity was observed with the soluble fraction obtained before hydrolysis of MBM and also observed with the relatively high MW hydrolysates from short treatment with trypsin and subtilisin. Low MW fractions obtained from by subtilisin treatment at 30–48 h also showed kaolin-settling ability, probably through a coagulation or charge neutralisation process.

scholarly journals Use of Meat Industry Waste in the Form of Meat-and-Bone Meal in Fertilising Maize (Zea mays L.) for Grain

2021 ◽  
Vol 13 (5) ◽  
pp. 2857
Author(s):  
Arkadiusz Stępień ◽  
Katarzyna Wojtkowiak ◽  
Ewelina Kolankowska
Keyword(s):  
Weather Conditions ◽  
Meat Industry ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Mineral N ◽  
Industry Waste ◽  
Yield And Quality ◽  
3.0 T ◽  
Effective Use

The processing of meat industry waste into meat-and-bone meal (MBM) provides the opportunity to use it as fertiliser in the cultivation of agricultural crops. This study was conducted in the years 2014–2017 at the Experimental Station in Tomaszkowo, Poland to assess MBM effects on yield and quality of maize cultivated for grain. An example of the effective use of nutrients contained in MBM applied at doses of 2.0 and 3.0 t ha is the cultivation of maize in 2016, which was affected by favourable weather conditions. The effect of the accumulation of MBM doses and, consequently, the provision of a greater amount of nutrients ensure sufficient amounts to obtain yields, greater than those provided by mineral fertilisation only. The macronutrient concentration in the maize grains following the application of MBM was similar to the composition of the grains of maize fertilised with mineral N, P and K fertilisers. With the MBM, micronutrients are introduced in amounts that are able to satisfy plants with these components, yet this study failed to demonstrate any effect of increased MBM doses on the concentration of the analysed elements in the maize grains.

scholarly journals High-Risk Biodegradable Waste Processing By Alkaline Hydrolysis

2011 ◽  
Vol 62 (3) ◽  
pp. 249-253 ◽  
Author(s):  
Sanja Kalambura ◽  
Neven Voća ◽  
Tajana Krička ◽  
Zoran Šindrak ◽  
Ana Špehar ◽  
...  
Keyword(s):  
High Risk ◽  
Alkaline Hydrolysis ◽  
Spongiform Encephalopathy ◽  
Waste Processing ◽  
Meat Industry ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Organic Fertiliser ◽  
Processing Technologies ◽  
Biodegradable Waste

High-Risk Biodegradable Waste Processing By Alkaline HydrolysisBiodegradable waste is by definition degraded by other living organisms. Every day, meat industry produces large amounts of a specific type of biodegradable waste called slaughterhouse waste. Traditionally in Europe, this waste is recycled in rendering plants which produce meat and bone meal and fat. However, feeding animals with meat and bone meal has been banned since the outbreaks of bovine spongiform encephalopathy (BSE). In consequence, new slaughterhouse waste processing technologies have been developed, and animal wastes have now been used for energy production. Certain parts of this waste, such as brains and spinal cord, are deemed high-risk substances, because they may be infected with prions. Their treatment is therefore possible only in strictly controlled conditions. One of the methods which seems to bear acceptable health risk is alkaline hydrolysis. This paper presents the results of an alkaline hydrolysis efficiency study. It also proposes reuse of the obtained material as organic fertiliser, as is suggested by the analytical comparison between meat and bone meal and hydrolysate.

Real-Time PCR Detection of Ruminant DNA

2004 ◽  
Vol 67 (3) ◽  
pp. 550-554 ◽  
Author(s):  
LUIS MENDOZA-ROMERO ◽  
EDWARD L. C. VERKAAR ◽  
PAUL H. SAVELKOUL ◽  
ARNOLD CATSBURG ◽  
HENK J. M. AARTS ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Bovine Spongiform Encephalopathy ◽  
Real Time ◽  
Real Time Pcr ◽  
Spongiform Encephalopathy ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Dna Targeting ◽  
Species Origin ◽  
Semiquantitative Method

To control the spread of bovine spongiform encephalopathy, several DNA methods have been described for the detection of the species origin of meat and bone meal. Most of these methods are based on the amplification of a mitochondrial DNA segment. We have developed a semiquantitative method based on real-time PCR for detection of ruminant DNA, targeting an 88-bp segment of the ruminant short interspersed nuclear element Bov-A2. This method is specific for ruminants and is able to detect as little as 10 fg of bovine DNA. Autoclaving decreased the amount of detectable DNA, but positive signals were observed in feeding stuff containing 10% bovine material if this had not been rendered in accordance with the regulations, i.e., heated at 134°C for 3 instead of 20 min.

scholarly journals Bovine meat and Bone Meal as an Economically Viable Alternative in Quail Feeding in the Final Phase

2016 ◽  
Vol 18 (spe) ◽  
pp. 7-12
Author(s):  
CC Pizzolante ◽  
JE Moraes ◽  
SK Kakimoto ◽  
FEL Budiño ◽  
C Móri ◽  
...  
Keyword(s):  
Viable Alternative ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Final Phase

scholarly journals Effect of isoproteinous feed on growth and survival of tilapia (Oreochromis niloticus) fry

2012 ◽  
Vol 10 (1) ◽  
pp. 169-174
Author(s):  
I Siddika ◽  
M Das ◽  
K R Sumi
Keyword(s):  
Wheat Flour ◽  
Rice Bran ◽  
Oreochromis Niloticus ◽  
Fish Meal ◽  
Cost Benefit Analysis ◽  
Cost Benefit ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Growth And Survival ◽  
Significant Difference

A four week experiment was conducted to evaluate the effect of isoproteinous (35%) feed prepared named diet 1, 2, 3 and 4 respectively by substituting 0%, 50% , 75% and 100% fish meal with meat and bone meal in view of preparing a cost effective tilapia (Oreochromis niloticus) fry feed. The four experimental diets (diet 1 to 4) each were randomly assigned to twelve hapas with three replications. Tilapia fry (0.011g) were randomly stocked in 100 fry/9ft² hapa and fed five times a day up to their apparent satiation. The results of the study showed that the weight gain, specific growth rate (SGR), the apparent net protein utilization value and survival (%) of the fish were significantly  (P<0.05) higher and better feed utilization with lower FCR value was observed in fish fed on the diet 4 where meat and bone meal was used as sole source of protein base. There was no significant difference between the carcass moisture and lipid of fish fed with different experimental diets. The results of the study indicated that diet 1 containing fish meal (45.15% Fish meal, 27.43% Rice bran and 27.43% Wheat flour) can be replaced by meat and bone meal containing diet 4 (54.06% Meat and bone meal, 22.97% Rice bran and 22.97% Wheat flour) with no adverse effects on growth and survival of O. niloticus. Before recommendation several trials will be necessary in different farms to be  sure of the reproducibility of the result obtained in the present experiment. Cost- benefit analysis reveals that the   meat and bone meal containing diet certainly provides cheaper feed.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12110   J. Bangladesh Agril. Univ. 10(1): 169–174, 2012  

Response of breeding sows to long term dietary supplementation with folic acid

1994 ◽  
Vol 1994 ◽  
pp. 153-153
Author(s):  
P.B. Lynch ◽  
P.J.A. Sheehy
Keyword(s):  
Folic Acid ◽  
Dietary Supplementation ◽  
Red Cell ◽  
Restricted Feeding ◽  
Meat And Bone Meal ◽  
Bone Meal ◽  
Soyabean Meal ◽  
Dietary Treatments

Dietary supplementation with folic acid has been shown to improve reproductive performance in sows (Lindemann 1993). However most studies have been for one cycle only and few have examined the effect of supplementation over several parities.One hundred and thirty four crossbred sows ranging in parity from 2 to 4 were selected at farrowing and randomly allocated to two dietary treatments of low and high supplemental folic acid (0 and 10 g per tonne, Roche Products Ltd.). Treatments were applied for the following three lactations and post weaning periods, two full pregnancies and to day 30 of the pregnancy following the third lactation. The diet fed contained barley, wheat, soyabean meal and meat and bone meal with nutrient levels of 14.0 MJ DE/kg and 1.02% lysine. Sows were individually penned throughout with restricted feeding in pregnancy (2.2 kg/day increasing to 2.5 kg/day in the final month), and ad libitum in lactation (approx 5.0 kg/day) and post weaning (approx 3.4 kg/day). Blood samples for determination of plasma and red cell folate were taken from 14 sows per treatment on days 4, 50 and 110 of one cycle. These were determined by a microbiological assay (modification of methods of Scott et al 1974 and Wilson and Home 1982).

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