Ubiquitous flocculation activity and flocculation production basis of the conglutination mud from Ruditapes philippinarum along the coast of China

Ruditapes philippinarum conglutination mud (RPM) is a typical waste by-product from manila clam R. philippinarum aquaculture. However, RPM from the clam at an aquaculture farm in Zhoushan, China, has been newly reported as a promising natural bioflocculant resource that contains effective flocculating polysaccharides from the clam associated bacteria. With an intent to figure out whether RPM flocculation activity is ubiquitous to the manila clam across a wide geographical range or only the Zhoushan location, and to explore the flocculation production basis and ultimately widen its exploitation scope, in this study, an extensive survey of RPMs from four representative locations along the coast of China was performed to determine their flocculation activity, polysaccharide constitution and bacterial community composition. Frozen preserved RPM samples from Zhoushan, Dalian, Weihai and Zhanjiang exhibited comparable flocculation activities (FRs) ranging from 61.9±2.4% to 73.2±0.9% at dosage of 8 g·L-1; while fresh RPMs from Zhoushan exhibited a much higher flocculation activity of 91.34±1.18% than its frozen counterpart. Polysaccharide extracts from the four locations showed similar monosaccharide constitutions to some extent. The geographical distribution led to certain variation in bacterial community structures. The similarity clustering of the polysaccharide compositions coincided with that of bacterial community structures from RPMs, suggesting that polysaccharides and respective bacterial communities might be the foundation of the flocculation activity for all RPMs. The overlapping OTUs across all the RPMs accounted for 44.6–62.22% of the overall sequences in each sample and contained the vast majority of the most abundant OTUs (Operational Taxonomic Units), forming a common "core microbiome" that is probably responsible for polysaccharide production and flocculation activity development.

Application of Extracellular Polymeric Substances Extracted From Wastewater Sludge for Reactive Dyes Removal

The aim of this study was to investigate the adsorption of three commercial reactive dyes using extracellular polymeric substances (EPS) extracted from the waste sludge of a beer wastewater treatment plant in Hanoi, Vietnam. EPS was extracted from sludge by HCHO-NaOH method and characterized by the measurement of kaolin flocculation activity, dry weight, chemical composition and functional groups. Adsorption of dyes on EPS was conducted by Jartest at different pH, reaction time and EPS dosage. The EPS was composed of 25% of sludge weight. The FTIR analysis showed the present of amine and carboxyl group in the EPS structure. The removal efficiencies of reactive dyes were high at pH below 6, the contact time was from 30 to 60 min and EPS dosage of 200 – 250 mg/L. At optimum condition, the removal efficiency of 85%, 99% and 99% were obtained for Reactive Yellow 176 (RY 176), Reactive Blue 21 (RB 21) and Reactive Red 241 (RR 241), respectively. The adsorption process could be described by both Langmuir and Fruendlich isothermal equations. The maximum dye adsorption capacity for RY 176, RB 21 and RR 241 was 0.50 g/g, 0.72 g/g and 0.95 g/g, respectively. This study revealed that the EPS in wastewater sludge can be utilized as an effective adsorbent for dyes removal, thereby, enhancing the value of sludge in wastewater treatment.

Rapid Production of a Novel Al(III) Dependent Bioflocculant Isolated From Raoultella ornithinolytica 160-1 and Its Application Combined With Inorganic Salts

An efficient bioflocculant-producing strain, Raoultella ornithinolytica 160-1, was identified by 16S rRNA and mass spectrometry analyses. Rapid production of bioflocculant EPS-160 was obtained with 10.01 g/(L⋅d) after optimized by response surface methodology. With the aid of Al(III), more than 90% flocculation activity of EPS-160 at 8 mg/L dosage was achieved in 5 min. Thus, this novel Al(III) dependent bioflocculant was used in combined with chemical coagulants AlCl3 to remove kaolin suspensions and wastewater treatment. The results indicated that the addition of EPS-160 in aggregation system not only largely improved the flocculation ability than the individual use of chemical flocculant (over 30 percent), but also overcome the decrease of flocculation activity due to the overdose of AlCl3 and maintained the optimum dosage of AlCl3 in a wide range (11–23 mg/L). The zeta potentials and EPS-160 structure indicated that both charge neutralization and bridging were the flocculation mechanism with kaolin. During the wastewater treatment, this composite flocculants consisted of EPS-160 and AlCl3 also had great performance for turbidity elimination. Moreover, with the properties of high flocculation activity, hyperthermal stability, pH tolerance and non-toxicity, EPS-160 shows great potential applications.

A Comparative Study between Bimetallic Iron@copper Nanoparticles with Iron and Copper Nanoparticles Synthesized Using a Bioflocculant: Their Applications and Biosafety

Nanotechnology addresses numerous environmental problems such as wastewater treatment. Ground water, surface water and wastewater that is contaminated by toxic organic, inorganic solutes and pathogenic microorganisms can now be treated through the application of nanotechnology. The study reports iron@copper (Fe@Cu) nanoparticles, iron nanoparticles (FeNPs) and copper nanoparticles (CuNPs) synthesized using a bioflocculant in a green approach technique. Characterization of the as-synthesized materials was achieved using analytical techniques such as Fourier transform-Infrared spectroscopy (FT-IR), Thermogravimetric analysis (TGA), Scanning Electron Microscope (SEM), Transmission Electron Microscopy (TEM), UV-Vis spectroscopy (UV-Vis) and X-ray diffraction (XRD). The presence of hydroxyl (–OH) and amine (–NH2) groups was shown by FT-IR spectroscopy studies and the as-synthesized material was shown to be thermostable. Elements such as oxygen, carbon, iron and copper were found to be abundant in Wt%. Absorption peaks were found between 200 and 390 nm wavelength and diffraction peaks at 2θ –29°, 33° and 35° for FeNPs, CuNPs and Fe@Cu, respectively. In their application, the effect of various parameters on the flocculation activity were evaluated. Both the CuNPs and (Fe@Cu) nanoparticles have shown the best flocculation activity at a concentration of 0.2 mg/mL with over 90% activity, while the dosage size with a concentration of 0.4 mg/mL was optimal for FeNPs. The FeNPs were found to be cation dependent, while CuNPs and Fe@Cu nanoparticles flocculate in the absence of a cation and flocculate both in acidic and alkaline pH. All the synthesized nanoparticles are thermostable and maintain flocculation activity above 80% at 100 °C. Both the Fe@Cu and CuNPs were found to be effective in removing dyes with the removal efficiency above 89% and were found to be effective in removal of chemical oxygen demand (COD) and biochemical oxygen demand (BOD) in Mzingazi river water and coal mine wastewater with over 80% removal efficiency. Moreover, the synthesized nanoparticles showed some remarkable antimicrobial properties when evaluated against Gram-positive and Gram-negative bacteria. The as-synthesized material was found to be safe to use at low concentration when verified against human embryonic cells (HEK293) and breast cancer cells (MCF7) and biodegradable.

Characterization of Two Cactus Formulation-Based Flocculants and Investigation on Their Flocculating Ability for Cationic and Anionic Dyes Removal

Dye invasion in wastewaters is undeniably one of the crucial environmental concerns in addition to the supplement of toxic synthetic chemical flocculants used for color removal using the conventional coagulation-flocculation process. With the aim to improve the flocculation stage in terms of reagents safety and ensure dyes removal, the present study explores the flocculating effectiveness of two natural, stable, and eco-friendly cactus formulations, namely 60 °C oven-dried (DP) and lyophilized (LP) cladodes. Both formulations were assessed to treat cationic (Methylene blue; MB) and anionic (Methyl Orange; MO) dye solutions as a substitution attempt for the currently questioned employed synthetic chemical flocculants. Obtained results demonstrate that, in conjunction with alum as coagulant, the lyophilized powder (LP) bio-based flocculant appears to be the most efficient cactus formulation, showing a significant color (83%) and a turbidity (69%) abatement for the cationic dye (MB) and, respectively, 63% and 62% for the anionic one (MO). Additionally, the flocculation activity of the LP formula remained high over an eight-month period of storage. Moreover, based on the Fourier transform infrared (FTIR) spectroscopic analysis and the chemical characterization of cactus formulations, the occurring flocculation mechanisms of the dye removal are presumed to be based on both adsorption and bridging phenomena. Further, the significant color and turbidity decline achieved upon the addition of the lyophilized cactus cladodes powder (LP), enhancing thus the coagulation performance of the alum-based coagulant, proved the effectiveness of this bio-flocculant compared to the commonly used chemical flocculant (polyacrylamide). Hence, it was suggested that lyophilized cactus cladodes as a natural flocculant could be one of the effective surrogates to chemical flocculants conventionally used in wastewater treatment for the sake of a safer and sustainable environment.

Wastewater Treatment by a Polymeric Bioflocculant and Iron Nanoparticles Synthesized from a Bioflocculant

Wastewater remains a global challenge. Various methods have been used in wastewater treatment, including flocculation. The aim of this study was to synthesize iron nanoparticles (FeNPs) using a polymeric bioflocculant and to evaluate its efficacy in the removal of pollutants in wastewater. A comparison between the efficiencies of the bioflocculant and iron nanoparticles was investigated. A scanning electron microscope (SEM) equipped with an energy-dispersive X-ray analyzer (EDX) and Fourier transform-infrared (FT-IR) spectroscopy were used to characterize the material. SEM-EDX analysis revealed the presence of elements such as O and C that were abundant in both samples, while FT-IR studies showed the presence of functional groups such as hydroxyl (–OH) and amine (–NH2). Fe nanoparticles showed the best flocculation activity (FA) at 0.4 mg/mL dosage as opposed to that of the bioflocculant, which displayed the highest flocculation activity at 0.8 mg/mL, and both samples were found to be cation-dependent. When evaluated for heat stability and pH stability, FeNPs were found thermostable with 86% FA at 100 °C, while an alkaline pH of 11 favored FA with 93%. The bioflocculant flocculated poorly at high temperature and was found effective mostly at a pH of 7 with over 90% FA. FeNPs effectively removed BOD (biochemical oxygen demand) and COD (chemical oxygen demand) in all two wastewater samples from coal mine water and Mzingazi River water. Cytotoxicity results showed both FeNPs and the bioflocculant as nontoxic at concentrations up to 50 µL.

Optimization of Fe@Cu Core–Shell Nanoparticle Synthesis, Characterization, and Application in Dye Removal and Wastewater Treatment

Green synthesis of core–shell nanoparticles is gaining importance nowadays as it is viewed as being environmental friendly and cost effective. The present study aimed to synthesize iron@copper core–shell nanoparticles using a polysaccharide-based bioflocculant from Alcalegenis faecalis and to evaluate its efficiency in dye removal and river water and domestic wastewater treatment. The synthesized samples were characterized by Fourier-transform infrared spectroscopy, X-ray diffraction, scanning electron microscopy, thermogravimetric analysis, transmission electron microscopy, and UV-Vis spectroscopy analysis. To optimize the best concentration for core–shell formation, different ratios of iron to copper were prepared. Sample 1 (S1) contained 1:3 iron to copper (Fe 25%–Cu 75%), sample 2 (S2) contained 1:1 iron to copper (Fe 50%–Cu 50%), and the third sample (S3) contained 3:1 iron to copper (Fe 75%–Cu 25%). The flocculation activity (FA) was above 98% at 0.2 mg/mL for all the samples and the samples flocculated well under acidic, alkaline, and neutral pH conditions. Sample 3 was shown to be thermostable, with flocculation activity above 90%, and samples 2 and 1 were also thermostable, but the flocculation decreased to 87 at 100 °C. All three samples revealed some remarkable properties for staining dye removal as the removal efficiency was above 89% for all dyes tested. The synthesized core–shell nanoparticles could remove nutrients such as total nitrogen and phosphate in both domestic wastewater and Mzingazi river water. Furthermore, high removal efficiency for chemical oxygen demand (COD) and biological oxygen demand (BOD) was also observed.