Effect of high altitude on nutrient digestibility, rumen fermentation and basal metabolism rate in Chinese Holstein cows on the Tibetan plateau

2013 ◽  
Vol 53 (3) ◽  
pp. 240 ◽  
Author(s):  
G. H. Qiao ◽  
C. Q. Yu ◽  
J. H. Li ◽  
X. Yang ◽  
X. Q. Zhu ◽  
...  
Keyword(s):  
High Altitude ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Basal Metabolism ◽  
Holstein Cows ◽  
Chinese Holstein ◽  
Metabolism Rate ◽  
Basal Metabolism Rate ◽  
Chinese Holstein Cows

Two experiments were conducted to investigate the effect of two altitudes (3600 and 1600 m) on nutrient digestibility, rumen fermentation and basal metabolism rate in Chinese Holstein cows. Experiment 1 was conducted to investigate the effect of high altitude (3600 m) on rumen fermentation, in vitro dry matter degradability, and nitrogen metabolism compared with low altitude (1600 m) in Chinese Holstein cows. Results indicated that total volatile fatty acids concentration, total gas production and gas coefficient a, b and c, efficiency of microbial protein synthesis and in vitro dry matter degradability were lower at 3600 m than 1600 m (P < 0.05). The number of protozoa and ammonia nitrogen concentration was higher at 3600 m than 1600 m (P < 0.05). We concluded that carbohydrate fermentation in the rumen was impaired, and ammonia nitrogen used for microbial nitrogen synthesis was also decreased at high altitude of 3600 m compared with 1600 m. Experiment 2 was conducted in vivo to investigate the effect of high altitude on apparent nutrient digestibility and basal metabolism rate in Chinese Holstein cows. Results indicated that the apparent digestibility of the diet’s dry matter, organic matter, neutral detergent fibre and acid detergent fibre was lower at 3600 m than those at 1600 m, respectively (P < 0.05). In Chinese Holstein cows, basal metabolism rate was increased with increasing level of altitude (P < 0.05). The results indicated that the high altitude of the Tibetan plateau impaired rumen fermentation and elevated the basal metabolism rate of Chinese Holstein cows.

Effect of diet synchrony on rumen fermentation, production performance, immunity status and endocrine in Chinese Holstein cows

2019 ◽  
Vol 59 (4) ◽  
pp. 664 ◽  
Author(s):  
G. H. Qiao ◽  
Z. G. Xiao ◽  
Y. Li ◽  
G. J. Li ◽  
L. C. Zhao ◽  
...  
Keyword(s):  
Organic Matter ◽  
Nitrogen Concentration ◽  
Rumen Fermentation ◽  
Production Performance ◽  
Holstein Cows ◽  
Productive Performance ◽  
Chinese Holstein ◽  
Chinese Holstein Cows

In order to maximise utilisation of nutrients for ruminants, researchers directed the supply of nitrogen and organic matter synchronously to the rumen. In the current research, two experiments were conducted, one using the in situ nylon bag technique and the other using in vivo study. In Experiment 1, dynamic degradation rates of organic matter (OM) and nitrogen of each feedstuff were determined using the in situ nylon bag technique. Accordingly, three diets were formulated with the same chemical components, but differing in synchrony index (0.74, 0.85 and 0.97). Experiment 2 was subsequently carried out in vivo to evaluate the influence of diet synchrony on rumen fermentation, productive performance, immunity status and endocrine in Chinese Holstein cows. Rumen degradability of OM and neutral detergent fibre (NDF) and total tract digestibility of OM were linearly increased with the increase of synchrony index, respectively (P &lt; 0.05). Total volatile fatty acids concentration of rumen fluid was increased linearly with the increase of synchrony index (P &lt; 0.05). Meanwhile, rumen ammonia nitrogen concentration and blood urea nitrogen concentration were decreased in cows with the increase of synchrony index, (P &lt; 0.05). Microbial crude protein production, the efficiency of microbial protein synthesis, and milk protein percentage, were linearly increased with the increase of synchrony index (P &lt; 0.05). Serum concentrations of prostaglandin E2 and immunoreactive fibronectin-γ were linearly decreased with the increase of diet synchrony index (P &lt; 0.05). The aforementioned results implicate that an increase of diet synchrony index could improve diet’s nitrogen utilisation, apparent digestibility of OM, and NDF. Additionally, it could promote rumen fermentation capacity, productive performance and immunity status in dairy cows.

The effects of feeding 3-nitrooxypropanol at two doses on milk production, rumen fermentation, plasma metabolites, nutrient digestibility, and methane emissions in lactating Holstein cows

2017 ◽  
Vol 57 (2) ◽  
pp. 282 ◽  
Author(s):  
Jennifer Haisan ◽  
Yun Sun ◽  
Leluo Guan ◽  
Karen A. Beauchemin ◽  
Allan Iwaasa ◽  
...  
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Methane Emissions ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Methane Yield ◽  
Holstein Cows ◽  
Ruminal Ph ◽  
Molar Proportion

The objective of this study was to determine the effects of feeding 3-nitrooxypropanol (NOP) in the total mixed ration of lactating Holstein cows on rumen fermentation, ruminal microbial population, enteric methane production, milk production, nutrient digestibility, and blood metabolites. Fifteen ruminally cannulated Holstein cows in mid to late lactation were used in a 3 × 3 Latin square design study with 28-day periods. Cows were fed a 60%-forage diet (dry matter basis) with 2500 (HIGH), 1250 (LOW) or 0 (CON) mg/day of NOP. After a 20-day diet adaptation period, dry matter intake (DMI) and milk yield were recorded daily. Rumen digesta and rumen fluid were collected on Days 21 and 28 and ruminal pH was determined on Days 23–28. Methane emissions were measured on Days 23–27 using the sulfur hexafluoride tracer gas technique. Faecal and blood samples were taken on Days 25–27. Feeding NOP at either dose did not affect DMI, milk production or bodyweight gain. Total concentration of volatile fatty acids in rumen fluid did not differ; however, there was a decrease in molar proportion of acetate and increase in molar proportion of propionate with feeding NOP in a dose-dependent manner. Microbial profile and ruminal pH were not affected by treatment. Apparent total-tract digestibility of DM (62.7% vs 58.4%; P < 0.05) and neutral detergent fibre (38.0% vs 30.7%; P < 0.05) were increased with the HIGH dose compared with CON. Feeding NOP reduced methane yield from 19.9 to 15.3 g/kg DMI for CON versus LOW (P < 0.05) and from 19.9 to 12.6 for CON versus HIGH (P < 0.05). Feeding NOP at 1250 or 2500 mg/day reduced methane yield and affected ruminal volatile fatty acid profile without compromising DMI or apparent total-tract nutrient digestibility.

Genetic polymorphism in 5′-flanking region of the lactoferrin gene and its associations with mastitis in Chinese Holstein cows

2009 ◽  
Vol 31 (4) ◽  
pp. 393-399 ◽  
Author(s):  
Hong-Mei WANG ◽  
Zhen-Xing KONG ◽  
Chang-Fa WANG ◽  
Jin-Ming HUANG ◽  
Qiu-Ling LI ◽  
...  
Keyword(s):  
Genetic Polymorphism ◽  
Holstein Cows ◽  
Chinese Holstein ◽  
Lactoferrin Gene ◽  
Chinese Holstein Cows ◽  
Flanking Region

Anti-mastitis SNV identification of NFκB1 in Chinese Holstein cows and the possible anti-inflammation role of NFκB1/p105 in bovine MECs

2020 ◽  
Vol 52 (11) ◽  
pp. 1191-1201
Author(s):  
Ling Chen ◽  
Rongfu Tian ◽  
Huilin Zhang ◽  
Xiaolin Liu
Keyword(s):  
Somatic Cell ◽  
Mrna Expression ◽  
Protein Content ◽  
Milk Protein ◽  
Holstein Cows ◽  
Chinese Holstein ◽  
Milk Protein Content ◽  
Chinese Holstein Cows ◽  
Anti Inflammation

Abstract NFκB1/p105 is the critical member of the NFκB family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NFκB1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NFκB1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in 547 Chinese Holstein cows, and explored the mRNA expression profiles of the NFκB family and ubiquitin ligases (βTrCP1, βTrCP2, KPC1, KPC2) in LPS-induced bovine mammary epithelial cells (MECs) by transcriptome-Seq. The association analysis showed that cows with SNV2-TT and SNV6-CC in the NFκB1 gene had significantly higher milk protein content (P &lt; 0.05), while cows with SNV5-TT in the NFκB1 gene had significantly lower somatic cell score (SCS), but CC genotype at SNV5 locus was not detected in our Holstein cows. The transcriptome-Seq results demonstrated the mRNA expression of NFκB1 was increased and peaked at 4 h post-induction, while the mRNA expressions of both KPC1 and BCL3 that promote the anti-inflammation function of NFκB1/p105 were decreased in LPS-induced bovine MECs. TNFAIP3, an inhibitor of both degradation and processing of p105 precursor, was markedly increased by more than 3 folds. Furthermore, bta-miR-125b which targets at the 3ʹUTR of TNFAIP3 was reduced by 50%. These results indicated that SNV5-TT of the NFκB1 gene with lower SCS may be an anti-mastitis genotype that could cope with infection more efficiently in Chinese Holstein cows. In addition, the anti-inflammation role of NFκB1/p105 seemed to be inhibited in LPS-induced-bovine MECs because the formation of the p50/p50 homodimer was arrested. This study provides a new perspective to understand the inflammatory mechanism in dairy mastitis.

scholarly journals Replacement of raw soybean with roasted soybean increased milk production in Holstein cows

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Gilson Sebastião Dias Júnior ◽  
Vítor Augusto Silveira ◽  
Ivan Júnior Ascari ◽  
Renata Apocalypse Nogueira Pereira ◽  
Marina de Arruda Camargo Danés ◽  
...  
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Degradation Kinetics ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Production Performance ◽  
Holstein Cows ◽  
Lower Protein ◽  
Inclusion Level ◽  
Improved Performance

ABSTRACT: The aim of this study was to evaluate the effect of total replacement of raw whole soybean (RAW) for roastedwhole soybean (ROS) on the production performance of Holstein cows. Two experiments were carried out usinga simple reversal design where RAW has been completely replaced by ROS. In experiment 1, 22 cows (175±60 days in milk)were used, and the dietary inclusion level of RAW or ROS was 3.7% of dry matter (DM). In experiment 2, 16 cows (130±50 days in milk)were used, and thedietary inclusion level of RAW or ROS was 11% of DM. In both experiments, ROS increased milk production by 1.1kgday-1 without changing fat and protein production. Dry matter intake or milk urea nitrogenwere not affected by dietary soy source. In experiment 2, plasma glucose concentration was decreased, and allantoin/creatinine ratio in urine tended to decreasein ROS. Experiment 2 also evaluated the nutrient digestibility and ruminal degradation kinetics of crude protein in two soybean sources. Roasting had no effect on the digestibility of DM, organic matter, and neutral detergent fiber. Roasted whole soybean hadgreater fraction B and lower protein degradation rate than did RAW; this showed that heat treatment was effective in increasing therumen undegradable amino acid flowto the animal, which suggesteda potential mechanism of action for improved performance observed in ROS.

Sign in / Sign up

Export Citation Format

Share Document