The effects of feeding 3-nitrooxypropanol at two doses on milk production, rumen fermentation, plasma metabolites, nutrient digestibility, and methane emissions in lactating Holstein cows

2017 ◽  
Vol 57 (2) ◽  
pp. 282 ◽  
Author(s):  
Jennifer Haisan ◽  
Yun Sun ◽  
Leluo Guan ◽  
Karen A. Beauchemin ◽  
Allan Iwaasa ◽  
...  
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Rumen Fluid ◽  
Methane Emissions ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Methane Yield ◽  
Holstein Cows ◽  
Ruminal Ph ◽  
Molar Proportion

The objective of this study was to determine the effects of feeding 3-nitrooxypropanol (NOP) in the total mixed ration of lactating Holstein cows on rumen fermentation, ruminal microbial population, enteric methane production, milk production, nutrient digestibility, and blood metabolites. Fifteen ruminally cannulated Holstein cows in mid to late lactation were used in a 3 × 3 Latin square design study with 28-day periods. Cows were fed a 60%-forage diet (dry matter basis) with 2500 (HIGH), 1250 (LOW) or 0 (CON) mg/day of NOP. After a 20-day diet adaptation period, dry matter intake (DMI) and milk yield were recorded daily. Rumen digesta and rumen fluid were collected on Days 21 and 28 and ruminal pH was determined on Days 23–28. Methane emissions were measured on Days 23–27 using the sulfur hexafluoride tracer gas technique. Faecal and blood samples were taken on Days 25–27. Feeding NOP at either dose did not affect DMI, milk production or bodyweight gain. Total concentration of volatile fatty acids in rumen fluid did not differ; however, there was a decrease in molar proportion of acetate and increase in molar proportion of propionate with feeding NOP in a dose-dependent manner. Microbial profile and ruminal pH were not affected by treatment. Apparent total-tract digestibility of DM (62.7% vs 58.4%; P < 0.05) and neutral detergent fibre (38.0% vs 30.7%; P < 0.05) were increased with the HIGH dose compared with CON. Feeding NOP reduced methane yield from 19.9 to 15.3 g/kg DMI for CON versus LOW (P < 0.05) and from 19.9 to 12.6 for CON versus HIGH (P < 0.05). Feeding NOP at 1250 or 2500 mg/day reduced methane yield and affected ruminal volatile fatty acid profile without compromising DMI or apparent total-tract nutrient digestibility.

scholarly journals Replacement of raw soybean with roasted soybean increased milk production in Holstein cows

2017 ◽  
Vol 47 (5) ◽  
Author(s):  
Gilson Sebastião Dias Júnior ◽  
Vítor Augusto Silveira ◽  
Ivan Júnior Ascari ◽  
Renata Apocalypse Nogueira Pereira ◽  
Marina de Arruda Camargo Danés ◽  
...  
Keyword(s):  
Milk Production ◽  
Dry Matter ◽  
Degradation Kinetics ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Production Performance ◽  
Holstein Cows ◽  
Lower Protein ◽  
Inclusion Level ◽  
Improved Performance

ABSTRACT: The aim of this study was to evaluate the effect of total replacement of raw whole soybean (RAW) for roastedwhole soybean (ROS) on the production performance of Holstein cows. Two experiments were carried out usinga simple reversal design where RAW has been completely replaced by ROS. In experiment 1, 22 cows (175±60 days in milk)were used, and the dietary inclusion level of RAW or ROS was 3.7% of dry matter (DM). In experiment 2, 16 cows (130±50 days in milk)were used, and thedietary inclusion level of RAW or ROS was 11% of DM. In both experiments, ROS increased milk production by 1.1kgday-1 without changing fat and protein production. Dry matter intake or milk urea nitrogenwere not affected by dietary soy source. In experiment 2, plasma glucose concentration was decreased, and allantoin/creatinine ratio in urine tended to decreasein ROS. Experiment 2 also evaluated the nutrient digestibility and ruminal degradation kinetics of crude protein in two soybean sources. Roasting had no effect on the digestibility of DM, organic matter, and neutral detergent fiber. Roasted whole soybean hadgreater fraction B and lower protein degradation rate than did RAW; this showed that heat treatment was effective in increasing therumen undegradable amino acid flowto the animal, which suggesteda potential mechanism of action for improved performance observed in ROS.

Comparison of banana flower powder and sodium bicarbonate supplementation on rumen fermentation and milk production in dairy cows

2016 ◽  
Vol 56 (10) ◽  
pp. 1650 ◽  
Author(s):  
Sungchhang Kang ◽  
Metha Wanapat ◽  
Anusorn Cherdthong ◽  
Kampanat Phesatcha
Keyword(s):  
Sodium Bicarbonate ◽  
Dairy Cows ◽  
Milk Production ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Dry Matter Intake ◽  
Microbial Nitrogen ◽  
Buffering Agent ◽  
Banana Flower

Four Holstein-Friesian crossbred dairy cows were randomly assigned according to a 2 × 2 factorial arrangement in a 4 × 4 Latin square design to study the effect of banana flower powder (BAFLOP) and sodium bicarbonate (NaHCO3) supplementation as rumen-buffering agents on rumen fermentation and milk production. The first factor was two ratios of roughage to concentrate (R : C) at 60 : 40 and 40 : 60, whereas the second was two sources of buffering agent (BAFLOP and NaHCO3) supplemented at 20 g/kg of dry matter intake. All cows were fed total dry matter intake at 25 g/kg bodyweight and untreated rice straw was used as a roughage source. Feeding R : C at 40 : 60 increased nutrient digestibilities, nitrogen absorption, allantoin excretion and absorption, microbial nitrogen synthesis, microbial crude protein and efficiency of microbial nitrogen synthesis in both BAFLOP- and NaHCO3-supplemented groups. BAFLOP supplementation could maintain ruminal pH as NaHCO3. Blood urea nitrogen, total volatile fatty acid and propionate, and milk yield increased in cows fed R : C at 40 : 60 both in BAFLOP and NaHCO3 supplementation, whereas acetate was relatively high in cows consuming R : C at 60 : 40. However, rumen microorganisms were similar among treatments whereas milk compositions were unchanged by the dietary treatments, except milk fat and total solids were the highest in cow consumed R : C at 60 : 40. The results suggested that feeding R : C at 40 : 60 resulted in improvement of nutrient digestibility, rumen fermentation efficiency and milk production, and supplementation of BAFLOP showed similar buffering capacity as NaHCO3. Therefore, BAFLOP could be used efficiently as a dietary rumen-buffering agent and promisingly deserves a replacement for bicarbonate sources in lactating dairy cows fed on a high concentrate diet.

Effect of high altitude on nutrient digestibility, rumen fermentation and basal metabolism rate in Chinese Holstein cows on the Tibetan plateau

2013 ◽  
Vol 53 (3) ◽  
pp. 240 ◽  
Author(s):  
G. H. Qiao ◽  
C. Q. Yu ◽  
J. H. Li ◽  
X. Yang ◽  
X. Q. Zhu ◽  
...  
Keyword(s):  
High Altitude ◽  
Dry Matter ◽  
Rumen Fermentation ◽  
Nutrient Digestibility ◽  
Basal Metabolism ◽  
Holstein Cows ◽  
Chinese Holstein ◽  
Metabolism Rate ◽  
Basal Metabolism Rate ◽  
Chinese Holstein Cows

Two experiments were conducted to investigate the effect of two altitudes (3600 and 1600 m) on nutrient digestibility, rumen fermentation and basal metabolism rate in Chinese Holstein cows. Experiment 1 was conducted to investigate the effect of high altitude (3600 m) on rumen fermentation, in vitro dry matter degradability, and nitrogen metabolism compared with low altitude (1600 m) in Chinese Holstein cows. Results indicated that total volatile fatty acids concentration, total gas production and gas coefficient a, b and c, efficiency of microbial protein synthesis and in vitro dry matter degradability were lower at 3600 m than 1600 m (P < 0.05). The number of protozoa and ammonia nitrogen concentration was higher at 3600 m than 1600 m (P < 0.05). We concluded that carbohydrate fermentation in the rumen was impaired, and ammonia nitrogen used for microbial nitrogen synthesis was also decreased at high altitude of 3600 m compared with 1600 m. Experiment 2 was conducted in vivo to investigate the effect of high altitude on apparent nutrient digestibility and basal metabolism rate in Chinese Holstein cows. Results indicated that the apparent digestibility of the diet’s dry matter, organic matter, neutral detergent fibre and acid detergent fibre was lower at 3600 m than those at 1600 m, respectively (P < 0.05). In Chinese Holstein cows, basal metabolism rate was increased with increasing level of altitude (P < 0.05). The results indicated that the high altitude of the Tibetan plateau impaired rumen fermentation and elevated the basal metabolism rate of Chinese Holstein cows.

scholarly journals Feed intake, nutrient digestibility, milk production and composition in dairy cows fed silage of wet brewers grain

2016 ◽  
Vol 37 (2) ◽  
pp. 1069 ◽  
Author(s):  
Leiliane Cristine de Souza ◽  
Maximiliane Alavarse Zambom ◽  
Claudete Regina Alcalde ◽  
Tatiane Fernandes ◽  
Deise Dalazen Castagnara ◽  
...  
Keyword(s):  
Organic Matter ◽  
Milk Production ◽  
Wheat Bran ◽  
Dry Matter ◽  
Milk Composition ◽  
Nutrient Digestibility ◽  
Neutral Detergent Fiber ◽  
Holstein Cows ◽  
Soybean Hulls ◽  
Ground Corn

The aim of this study was to evaluate the supply of wet brewers grain silage with and without nutrient additives in ensiling (wheat bran, soybeans hulls and ground corn), and the effect of additives on the intake and digestibility of dry matter and nutrients as well as the efficiency, production and milk composition of Holstein cows. Four multiparous Holstein cows were used, with an average milk production of 25 liters per day. The cows were randomly assigned, via the Latin Square design (4x4), to one of four experimental diets. The experimental period was 21 days. The results obtained in the experiment were evaluated by analysis of variance, Fisher’s F test and Tukey’s test at 5 % probability. The experimental diets consisted of wet brewers grain silage alone, and this silage with the addition of wheat bran, soybean hulls or ground corn. The diets did not affect the initial body weight of the animals. Diets containing wheat bran and ground corn provided a higher intake of dry matter (21.78 and 21.50 kg day-1) and organic matter (20.42 and 20.22 kg day-1) compared to the wet brewers grain silage alone and the diet containing soybean hulls. The results observed for dry matter (636.96 and 632.96 g kg-1), organic matter (659.16 and 654.35 g kg-1 DM), crude protein (712.84 and 717.79 g kg-1 DM) and neutral detergent fiber (598.99 and 538.90 g kg-1 DM) when cows were fed diets containing soybean hulls and ground corn, respectively, were higher than other diets. The different diets did not change daily milk production, milk production corrected for 3.5 % fat or milk composition. The milk urea nitrogen from cows fed ground corn diets was a lower concentration (17.67 mg dL-1), however, it was still above the suitable range (10 to 14 mg dL-1). Providing wet brewers grain silage with soybeans hulls or ground corn to Holstein cows increased the digestibility of dry matter and nutrients. However, the use of silages with wheat bran, soybean hulls or ground corn did not alter the efficiency, production or milk composition of cows fed with these silages.

scholarly journals 49 Effects of in-vitro digestibility in cannulated steers when supplemented different levels of glycerol

2020 ◽  
Vol 98 (Supplement_2) ◽  
pp. 55-56
Author(s):  
Noheli Gutierrez ◽  
Jamie A Boyd
Keyword(s):  
Dry Matter ◽  
Cold Water ◽  
Rumen Fluid ◽  
Latin Square ◽  
Optimal Level ◽  
Nutrient Digestibility ◽  
In Vitro Digestibility ◽  
Study Results ◽  
Different Levels

Abstract A study was conducted to evaluate effects of increasing concentration of food grade glycerol on rumen environment and nutrient digestibility. Three ruminally cannulated Jersey steers were used in this study. The study was conducted from March to May 2019. Experimental design was a 3x3 Latin square with a 2wk adjustment period followed by a 1wk collection period. Diet was coastal bermudagrass hay based. Different forage types were introduced in the incubation process to evaluate digestibility. Glycerol was administered once a day at 0, 15, or 20% of DMI (dry matter intake). dNDF (digestible NDF) and dDM (digestible dry matter) was determined using an ANKOM Daisy II incubator inoculated with 200g fresh rumen fluid and incubated for 12, 24, 48 and 72 h at 39°C. Each vessel contained ground forage samples in filter bags in triplicate. After incubation, filter bags were rinsed with cold water and dried for 24h in a 55°C forced air oven. Data were analyzed using the Proc MIXED procedure of SAS version 9.4. There was no difference dNDF in effect of different levels of glycerol between forage types by diet. But a numerical tendency was observed that dNDF was decreased at 20% inclusion rates in comparison to 0 and 15% inclusion of glycerol in the diet. Neither steer nor run was significantly different in the study. However as expected digestibility over time was significantly different (P &lt; 0.001). A significant increase was observed in DMI with the increased levels of glycerol in the diet (P = 0.003), both the 15% and 20% levels of glycerol increased in DMI in comparison to the control (0%). It appears based on these study results that digestibility may be inhibited, as levels of dietary glycerol increase in the diet and more work needs to be done to find the optimal level of glycerol supplementation.

scholarly journals Concentrate containing calcium salts of fatty acids rich in polyunsaturated fatty acids can change rumen fermentation in grazing goats

2018 ◽  
Vol 39 (6) ◽  
pp. 2621
Author(s):  
Ludmila Couto Gomes ◽  
Claudete Regina Alcalde ◽  
Julio Cesar Damasceno ◽  
Luiz Paulo Rigolon ◽  
Ana Paula Silva Possamai ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Latin Square ◽  
Rumen Fermentation ◽  
Ruminal Fermentation ◽  
Calcium Salts ◽  
Linear Effect ◽  
Concentrate Supplementation ◽  
Ruminal Ph

Feeding goats with calcium salts of fatty acids (CSFA) can supply ruminants with lipids, with minimal effects on ruminal fermentation and fiber digestibility. However, there is a shortage of information on the effect of CSFA on characteristics of rumen fermentation in grassland goats. Thus, the present study aimed to assess the addition of CSFA to concentrate on the parameters of rumen fermentation of grazing goats. Five rumen cannulated goats were distributed in a Latin square 5x5 design (treatments: 0%, 1.5%, 3.0%, 4.5% and 6.0% CSFA. The pH, ammonia N and volatile fatty acids (VFA) content were analyzed in the ruminal fluid at 0, 2, 4, 6 and 8 hours after concentrate supplementation. The pH and ammonia N concentration showed a linear effect with the addition of CSFA. There was no effect observed for the VFA molar concentration after grazing goats were fed with the experimental diet. In conclusion, further research is needed to investigate the addition of CSFA to goat diets because there is evidence that CSFA increases ruminal pH and decreases excess ruminal ammonia without changing the VFA concentration in the rumen fluid.

Intraruminal administration ofMegasphaera elsdeniimodulated rumen fermentation profile in mid-lactation dairy cows

2011 ◽  
Vol 79 (1) ◽  
pp. 16-25 ◽  
Author(s):  
Qendrim Zebeli ◽  
Sarah J Terrill ◽  
Alberto Mazzolari ◽  
Suzanna M Dunn ◽  
Wen Z Yang ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Dairy Cows ◽  
Milk Yield ◽  
Volatile Fatty Acids ◽  
Rumen Fluid ◽  
Plasma Concentrations ◽  
Rumen Fermentation ◽  
Holstein Cows ◽  
Plasma Metabolites ◽  
Fermentation Profile

This study evaluated the effects of intraruminal administration ofMegasphaera elsdeniion ruminal fermentation patterns, the profile of plasma metabolites, and milk yield and composition of mid-lactation dairy cows. Eight primiparous, ruminally cannulated Holstein cows were arranged in a paired 2×2 crossover design. Cows were randomly assigned to one of two treatments: 1) intraruminal inoculation of 35 ml suspension per day ofM. elsdeniiATCC 25940 (MEGA), containing 108cfu/ml of bacteria, dissolved in 35 ml of saline (0·15m), or 2) carrier alone (35 ml saline; CTR). Both postprandial and preprandial rumen volatile fatty acids (VFA) and plasma metabolite measurements were analysed. Postprandial VFA patterns were affected the most, with butyrate (P<0·01) and valerate (P<0·01) proportions increasing, and acetate (P<0·01), isobutyrate (P=0·05) and isovalerate (P<0·01) decreasing in MEGA cows. Preprandial data measured at various days showed that MEGA dosage tended to increase the molar proportion of propionate (P=0·09) and lower the acetate to propionate ratio (P=0·07) in the rumen fluid. There was no effect of treatment on rumen pH and on the concentration of lactate in the rumen as well as on selected preprandial plasma metabolites. Postprandial plasma concentrations of cholesterol tended to increase (P=0·07) in MEGA cows compared with CTR. Concentrations of non-esterified fatty acids (NEFA) in the plasma were lower in MEGA cows after the morning feeding (P<0·01). Sampling hour also affected plasma NEFA in this study. Plasma β-hydroxybutyrate (BHBA) were not affected by the treatment (P>0·05); however, after the morning feeding BHBA concentration was increased in both groups of cows. Dry matter intake and milk yield and composition were not affected by treatment. In conclusion, results indicate thatM. elsdeniihas the potential to modulate the rumen fermentation profile in mid-lactation Holstein cows, but these effects were only slightly reflected in changes in plasma metabolites and milk composition.

Sign in / Sign up

Export Citation Format

Share Document