Effect of age, retinol, and cholecalciferol on carcass fat and adipocyte number and size in growing lambs

1997 ◽  
Vol 48 (1) ◽  
pp. 7 ◽  
Author(s):  
E. Payne ◽  
S. Watkins
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Adipocyte Differentiation ◽  
Age Effects ◽  
Cell Diameter ◽  
Treatment Groups ◽  
Steady Growth ◽  
Total Fat ◽  
Effect Of Age

To determine whether hyperplasia occurs in young growing meat lambs when fat is laid down we measured the changes in fat parameters with age in Coopworth × Dorset lambs and, in parallel, ran groups treated with retinol and cholecalciferol (reported inhibitors of adipocyte differentiation in vitro), which were slaughtered at 33–34 weeks of age. The lambs were fed a pelleted ration at 30 g/kg body weight from 16 weeks of age and serially slaughtered for age effects in groups of 4, whereas the treatment groups contained 6 lambs. Some lambs developed urethral blockage from pellet feeding so the experiment was terminated early at 33–34 weeks. The lambs showed a steady growth rate and an increase in the proportion of fat in the carcass, total carcass fat, and subcutaneous adipocyte diameter at 25–29 weeks of age. The total number of adipocytes in the carcass derived from diameter measurements on subcutaneous adipocytes and the total carcass fat showed no significant changes. Retinol and cholecalciferol caused significant reductions in cell diameter and increases in total adipocytes but little change in total fat.

scholarly journals In Vivo Radioprotective Activity of Cell-Permeable Bifunctional Antioxidant Enzyme GST-TAT-SOD against Whole-Body Ionizing Irradiation in Mice

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Jianru Pan ◽  
Huocong He ◽  
Ying Su ◽  
Guangjin Zheng ◽  
Junxin Wu ◽  
...  
Keyword(s):  
Growth Rate ◽  
Antioxidant Activity ◽  
Body Weight ◽  
Whole Body ◽  
White Pulp ◽  
Radioprotective Activity ◽  
Significant Difference ◽  
Wbc Count

GST-TAT-SOD was the fusion of superoxide dismutase (SOD), cell-permeable peptide TAT, and glutathione-S-transferase (GST). It was proved to be a potential selective radioprotector in vitro in our previous work. This study evaluated the in vivo radioprotective activity of GST-TAT-SOD against whole-body irradiation. We demonstrated that intraperitoneal injection of 0.5 ml GST-TAT-SOD (2 kU/ml) 2 h before the 6 Gy whole-body irradiation in mice almost completely prevented the splenic damage. It could significantly enhance the splenic antioxidant activity which kept the number of splenic white pulp and consequently resisted the shrinkage of the spleen. Moreover, the thymus index, hepatic antioxidant activity, and white blood cell (WBC) count of peripheral blood in irradiated mice pretreated with GST-TAT-SOD also remarkably increased. Although the treated and untreated irradiated mice showed no significant difference in the growth rate of animal body weight at 7 days postirradiation, the highest growth rate of body weight was observed in the GST-TAT-SOD-pretreated group. Furthermore, GST-TAT-SOD pretreatment increased resistance against 8 Gy whole-body irradiation and enhanced 30 d survival. The overall effect of GST-TAT-SOD seemed to be a bit more powerful than that of amifostine. In conclusion, GST-TAT-SOD would be a safe and potentially promising radioprotector.

Anti-Müllerian hormone reduces growth rate without altering follicular survival in isolated caprine preantral follicles cultured in vitro

2017 ◽  
Vol 29 (6) ◽  
pp. 1144 ◽  
Author(s):  
R. M. P. Rocha ◽  
L. F. Lima ◽  
I. R. Brito ◽  
G. M. Silva ◽  
H. H. V. Correia ◽  
...  
Keyword(s):  
Growth Rate ◽  
Cytochrome P450 ◽  
Hormone Receptors ◽  
Mrna Levels ◽  
Follicular Growth ◽  
Steroid Secretion ◽  
Preantral Follicles ◽  
Treatment Groups ◽  
Cytochrome P450 Family

The aim of the present study was to evaluate the effect of anti-Müllerian hormone (AMH), with and without FSH, on the in vitro development of isolated caprine preantral follicles, as well as follicular steroid production and mRNA levels of AMH, hormone receptors (AMH and FSH), CYP19A1 (cytochrome P450, family 19, subfamily A, polypeptide 1), CYP17 (cytochrome P450, family 17, subfamily A, polypeptide 1), HSD3B (3-beta-hydroxysteroid dehydrogenase) and Myc (myelocytomatosis oncogene). Isolated secondary follicles were cultured in minimum essential medium alpha (α-MEM+) alone or supplemented with 50 ng mL–1 AMH and/or 100 ng mL–1 FSH added sequentially on different days of culture. Follicles were cultured for a total of 18 days, with different media during the first (Days 0–9) and second (Days 10–18) halves of the culture period, resulting in six treatment groups, as follows: α-MEM+/α-MEM+, FSH/FSH, AMH/AMH, AMH+FSH/AMH+FSH, AMH/FSH, and FSH/AMH. Follicle development was evaluated on the basis of follicular growth, oocyte maturation and steroid secretion. There was a decrease in follicular growth rate in the AMH, AMH + FSH and AMH/FSH treatment groups compared with α-MEM+ and FSH treatment groups (P < 0.05). However, the different culture conditions had no effect on rates of meiotic resumption and steroid secretion (P > 0.05). Moreover, follicles cultured in the presence of FSH had lower levels of AMH receptor type II (AMHRII) mRNA compared with non-cultured control (freshly isolated follicles), and the AMH and AMH/FSH treatment groups. In conclusion, AMH reduces the follicular growth rate of isolated goat preantral follicles in vitro without affecting follicular survival.

scholarly journals Downregulation of RelA (p65) by Rapamycin Inhibits Murine Adipocyte Differentiation and Reduces Fat Mass of C57BL/6J Mice despite High Fat Diet

ISRN Obesity ◽  
2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Peter D. Ray ◽  
Reid A. Maclellan ◽  
Jin He ◽  
Zhigang Liu ◽  
Jianguo Wu
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Adipocyte Differentiation ◽  
Mammalian Target Of Rapamycin ◽  
Subcutaneous Fat ◽  
Immunosuppressive Agent ◽  
Dependent Manner ◽  
High Fat

Rapamycin (RAPA) is a clinical immunosuppressive agent first reported in the literature in 1975 after its discovery in a soil sample from the island of Rapa Nui. Aside from the well-documented effects of RAPA on cell division and immunologic response, the literature reveals it to have negative effects on adipocyte and osteocyte differentiation as well. Understanding of the molecular effects of RAPA on cell differentiation is fragmentary in regard to these cell lineages. In this paper, we examined a potential mechanism for RAPA’s effects on adipocyte differentiation in vitro and in vivo. The data point to a unique role of Rel A (p65)—a component of the NF-κB system—in mediating this event. In murine adipose derived stem cell cultures (muADSCs) from C57BL/6J mice, RAPA was found to selectively downregulate RelA/p65, mammalian target of rapamycin (mTOR), and do so in a dose-dependent manner. This implies a novel role for RelA in adipocyte biology. Intracellular lipid accumulation—as subjectively observed—was also decreased in muADSCs treated with RAPA. Mice treated with RAPA had reduced overall body weight and reduced size of both intraabdominal and subcutaneous fat pads. When treated with RAPA, mice fed a high fat diet did not develop obesity and were not different from their regular diet controls in terms of body weight. These results suggested that RAPA inhibits adipogenesis and lipogenesis of muADSCs resulting in a prevention of obesity in C57BL/6J mice. This inhibition is strong enough to negate the effects of a high fat diet and seems to act by downregulating the RelA/p65 mTOR signaling pathway—a key component of the NF-κB family.

scholarly journals Effect of Non-Conventional and Conventional Fodder on Growth Performance of Goats in Farmers Field Condition

2007 ◽  
Vol 7 ◽  
pp. 15
Author(s):  
M. R. Tiwari ◽  
N. L. Tamrakar ◽  
H. R. Shrestha
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Dry Matter ◽  
Average Daily Gain ◽  
Maize Stover ◽  
Male And Female ◽  
Treatment Groups ◽  
Concentrate Mixture ◽  
Similar Body ◽  
Daily Gain

The present experiment was carried out on farmers’ goats in their sheds in Rayale village of Kavre district for 150 days. Eighteen khari goats (both male and female), 8-12 months old and having similar body weight were randomly divided into three groups comprising of six individuals in each group. Three types of rations were formulated for the experimental goats. Feed intake by experimental goats in terms of green and dry matter was significantly different (P&lt;0.05) between the treatment groups. Growth rate of the goats fed with top dressed maize stover (treatment 2) was higher than the treatment 3 (seasonal fodder adlib + 200 g concentrate mixture) and treatment 1 (1/2 maize stover silage + seasonal fodder adlib + 200 g concentrate mixture). The lowest average daily gain (ADG) was found in treatment 3 where goats were fed conventional fodder and grasses supplemented with concentrate (18.4 g) followed by treatment 1 (24.6 g). <i> Nepal Journal of Science and Technology </i> Vol. 7 2006

Effect of Hematoporphyrin « in Vivo » and « in Vitro » on ATPC+ Tumor Cells

1968 ◽  
Vol 54 (5) ◽  
pp. 361-368
Author(s):  
Giorgio Cittadini ◽  
Tommaso De Cata ◽  
Carlo Gubinelli
Keyword(s):  
Growth Rate ◽  
Weight Loss ◽  
Body Weight ◽  
Drug Toxicity ◽  
Body Weight Loss ◽  
Ascites Tumor ◽  
Once Daily ◽  
Fluid Interaction

ATPC+ ascites tumor transplants of different size were performed in NMRI mice. Hematoporphyrin chlorhydrate (Hp) was administered i.p. once daily during the seven days following transplantation. In the first series « in vivo » (1.9 x 106 cells; 0.1, 0.3, 0.9 mg of Hp), body weight, ascitogenic time (Tasc) and total ascitic volume (TV) were determined. Hematoporphyrin-treated animals showed, after the apprearance of the ascites, a body weight loss superior to controls, due to the drug toxicity caused by Hp vs. ascitic fluid interaction. Neither Tasc nor TV were significantly modified. In the second series « in vivo » (2.3 x 104 or 2.3 x 102 cells; 0.3 mg Hp), body weight loss was lower, Tasc was significantly increased and also the survival varied. In a third experiment ATPC+ cells were incubated « in vitro » with Hp in a range from 102 to 108 molecules/cell). No effect was observed on growth rate when the cells were transplanted into the host.

scholarly journals Efficacy of Daptomycin against Bacillus anthracis in a Murine Model of Anthrax Spore Inhalation

2010 ◽  
Vol 54 (10) ◽  
pp. 4471-4473 ◽  
Author(s):  
Henry S. Heine ◽  
Jennifer Bassett ◽  
Lynda Miller ◽  
Bret K. Purcell ◽  
W. Russell Byrne
Keyword(s):  
Body Weight ◽  
Bacillus Anthracis ◽  
Murine Model ◽  
Day Treatment ◽  
Survival Rates ◽  
Treatment Groups ◽  
Anthrax Spore ◽  
Phosphate Buffered Saline

ABSTRACT Daptomycin demonstrated in vitro (MIC90, 4 μg/ml) and in vivo activities against Bacillus anthracis. Twice-daily treatment with a dose of 50 mg/kg of body weight was begun 24 h after challenge and continued for 14 or 21 days; results were compared to those for controls treated with phosphate-buffered saline or ciprofloxacin. Day 43 survival rates were 6/10 mice for the 14-day and 9/10 mice for the 21-day treatment groups, compared to survival with ciprofloxacin: 8/10 and 9/10 mice, respectively. Culture results from tissues removed at the termination of the experiment were negative.

scholarly journals Pengaruh Penambahan Sargassum sp. dan Inositol dalam Pakan terhadap Pertumbuhan dan Daya Tahan Juvenil Ikan Gurami (Osphronemus gouramy Lac.)

2017 ◽  
Vol 16 (2) ◽  
pp. 72
Author(s):  
Dwi Lestari ◽  
E. L. Widiastuti ◽  
N. Nurcahyani ◽  
G. N. Susanto
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Survival Rate ◽  
Body Length ◽  
Growth Parameters ◽  
Freshwater Fishes ◽  
The Body ◽  
Body Width ◽  
Treatment Groups ◽  
Commercial Feed

Gourami (Osphronemus gouramy Lac.) as one of the freshwater fishes, are easily to be cultured but slow in growth rate. One of the ways to enhance their growth rate is by feeding them with high nutritious food. Sargassum sp. and inositol are known compound able to stimulate the growth of some fishes. The study was conducted to determine the effect of Sargassum sp and inositol on commercial feed to the growth rate and survival of juvenile gouramy. The study was conducted from January to April 2015 in Aquatic Laboratory of Biology Department - University of Lampung. Completely randomized designed was used with 4 treatment groups and 5 replications. Anova and Tukey at 5% levels were applied to this study. The result indicated that addition of inositol and Sargassum sp on commercial food significantly affected the gourami growth parameters such as the body weight, body length, body width as well as juvenile gouramy spesific growth rate and their survival rate.

scholarly journals Effect ofAchyranthes bidentataBlume on 3T3-L1 Adipogenesis and Rats Fed with a High-Fat Diet

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Sang Deog Oh ◽  
Mihyun Kim ◽  
Byung-Il Min ◽  
Gi Soon Choi ◽  
Sun-Kwang Kim ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Adipocyte Differentiation ◽  
Body Weight Gain ◽  
Water Extract ◽  
High Fat ◽  
Achyranthes Bidentata ◽  
Reduced Body ◽  
Differentiation Model

The present study investigated the antiobesity effect ofAchyranthes bidentataBlume root water extract in a 3T3-L1 adipocyte differentiation model and rats fed with a high-fat diet. To investigate the effect ofAchyranthes bidentataBlume on adipogenesisin vitro, differentiating 3T3-L1 cells in adipocyte-induction media were treated every two days withAchyranthes bidentataBlume at various concentrations (1 to 25 μg/mL) for eight days. We found thatAchyranthes bidentataBlume root inhibited 3T3-L1 adipocyte differentiation without affecting cell viability, and Western blot analysis revealed that phospho-Akt expression was markedly decreased, whereas there was no significant change in perilipin expression. Furthermore, administration ofAchyranthes bidentataBlume root (0.5 g/kg body weight for six weeks) to rats fed with a high-fat diet significantly reduced body weight gain without affecting food intake, and the level of triglyceride was significantly decreased when compared to those in rats fed with only a high-fat diet. These results suggest thatAchyranthes bidentataBlume root water extract could have a beneficial effect on inhibition of adipogenesis and controlling body weight in rats fed with a high-fat diet.

The effects of cross-fostering on growth rate and post-weaning behavior of segregated early-weaned piglets

2000 ◽  
Vol 80 (4) ◽  
pp. 533-538 ◽  
Author(s):  
S. Giroux ◽  
S. Robert ◽  
G.-P. Martineau
Keyword(s):  
Growth Rate ◽  
Body Weight ◽  
Skin Lesions ◽  
Early Weaning ◽  
Weaned Piglets ◽  
Eating Frequency ◽  
Treatment Groups ◽  
Key Words Pig ◽  
Cross Fostering ◽  
All Treatment

Cross-fostering involving piglets older than 2 d of age is often used in segregated early weaning (SEW) units to increase piglets' body weight homogeneity. This study was conducted to document the effects of such cross-fostering on weight gain, skin lesions, and post-weaning behavior of SEW piglets. Cross-fostering was done at 6 ± 1 d of age, in half of the 32 litters studied, by exchanging two piglets between pairs of litters. Piglets (n = 256) were weighed at birth, fostering, weaning (day 18 ± 1), and every week during the next month. The behavior of piglets was video-recorded during 3 h after weaning, and during 1 h on days 19, 20, 22, 24, 31, 38 and 45. Adopted piglets gained only 76% of the weight of non-adopted piglets between fostering and weaning (P < 0.001) and this difference persisted until day 45 (P < 0.05). Piglets from fostered litters fought less than control piglets during their first 2 d in nursery pens (P < 0.01) and skin lesions tended to be less frequent (P < 0.1). In all treatment groups, eating frequency was low on days 18 and 19 and increased abruptly on day 20. In conclusion, fostering impaired growth of piglets, but also facilitated their adaptation to unacquainted piglets after weaning. Key words: Pig, fostering, behavior, growth, welfare, segregated early weaning

scholarly journals Effect of Age at Castration on Beef Calf Performance

EDIS ◽  
2013 ◽  
Vol 2013 (3) ◽  
Author(s):  
Amie Imler ◽  
Todd Thrift ◽  
Matt Hersom ◽  
Joel Yelich
Keyword(s):  
Growth Rate ◽  
Growth Rates ◽  
Weaning Weight ◽  
Calf Growth ◽  
Treatment Groups ◽  
Fact Sheet ◽  
Calf Performance ◽  
Animal Sciences ◽  
Effect Of Age

A study in Florida was conducted to examine the issue of age at castration to determine if castration timing resulted in significant differences in growth rate and weaning weight in nursing calves. In addition, the study included a comparison between Angus and Brangus calves in the treatment groups to determine if there was a breed by castration effect. No differences in calf growth rates were observed in early compared to late castration. This 4-page fact sheet was written by Amie Imler, Todd Thrift, Matt Hersom, and Joel Yelich, and published by the UF Department of Animal Sciences, March 2013. http://edis.ifas.ufl.edu/an289

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