Ultrahigh-resolution, frequency-resolved resonance fluorescence imaging with a monoisotopic mercury atom cell

1999 ◽  
Vol 86 (10) ◽  
pp. 5337-5341 ◽  
Author(s):  
A. A. Podshivalov ◽  
M. R. Shepard ◽  
O. I. Matveev ◽  
B. W. Smith ◽  
J. D. Winefordner
Keyword(s):  
Fluorescence Imaging ◽  
Mercury Atom ◽  
Resonance Fluorescence ◽  
Ultrahigh Resolution

Lipid-Encapsulated Fe3O4 Nanoparticles for Multimodal Magnetic Resonance/Fluorescence Imaging

2020 ◽  
Vol 3 (7) ◽  
pp. 6785-6797
Author(s):  
Linlin Zhang ◽  
Sheng Tong ◽  
Qingbo Zhang ◽  
Gang Bao
Keyword(s):  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
Fe3o4 Nanoparticles ◽  
Resonance Fluorescence

Sub-Doppler Spectral Resolution and Improved Sensitivity in a Cesium Resonance Fluorescence Imaging Monochromator

2002 ◽  
Vol 56 (6) ◽  
pp. 677-681 ◽  
Author(s):  
Nathan C. Pixley ◽  
Tiffany L. Correll ◽  
D. Pappas ◽  
Benjamin W. Smith ◽  
James D. Winefordner
Keyword(s):  
Fluorescence Imaging ◽  
Spectral Resolution ◽  
Hollow Cathode ◽  
Limit Of Detection ◽  
Line Emission ◽  
Image Intensifier ◽  
Chemical Imaging ◽  
Resonance Fluorescence ◽  
Excitation Scheme ◽  
Hollow Cathode Lamp

Improvements in spectral resolution and sensitivity of a cesium based resonance fluorescence imaging monochromator (RFIM) are described. Alignment of the two lasers used in the excitation scheme in a co-propagating geometry have resulted in a detector spectral bandpass (270 MHz) that is narrower than the Doppler broadened profile (380 MHz) of cesium vapor at room temperature. Improvements in the experimental design, namely the addition of an image intensifier and cooled charge-coupled device (CCD) camera, have resulted in increased sensitivity; we report a limit of detection of 108 photons per pixel limited by image intensifier noise. Images of the cesium D2 line emission (852.12 nm) from a cesium hollow cathode lamp were compared to an argon emission line (4Sº1/2 → 4P3/2) at 852.14 nm from a silver/argon hollow cathode lamp as a demonstration of the spectral resolution. The ability of the RFIM to discriminate between photons exciting the F = 4 vs. F = 3 ground state hyperfine level (a frequency difference of 9.2 GHz) of the cesium 62S1/2 state was also investigated to further demonstrate the spectral selectivity. The potential use of the described RFIM for chemical imaging applications is discussed.

scholarly journals Retro-enantio isomer of angiopep-2 assists nanoprobes across the blood-brain barrier for targeted magnetic resonance/fluorescence imaging of glioblastoma

2021 ◽  
Vol 6 (1) ◽  
Author(s):  
Ruoxi Xie ◽  
Zijun Wu ◽  
Fanxin Zeng ◽  
Huawei Cai ◽  
Dan Wang ◽  
...  
Keyword(s):  
Magnetic Resonance ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Ex Vivo ◽  
Malignant Gliomas ◽  
Resonance Fluorescence ◽  
Malignant Tumours ◽  
Nir Fluorescence

AbstractGlioblastoma (GBM), one of the most common primary intracranial malignant tumours, is very difficult to be completely excised by surgery due to its irregular shape. Here, we use an MRI/NIR fluorescence dual-modal imaging nanoprobe that includes superparamagnetic iron oxide nanoparticles (SPIONs) modified with indocyanine (Cy7) molecules and peptides (ANG or DANG) to locate malignant gliomas and guide accurate excision. Both peptides/Cy7-SPIONs probes displayed excellent tumour-homing properties and barrier penetrating abilities in vitro, and both could mediate precise aggregation of the nanoprobes at gliomas sites in in vivo magnetic resonance imaging (MRI) and ex vivo near-infrared (NIR) fluorescence imaging. However, compared with ANG/Cy7-SPIONs probes, DANG/Cy7-SPIONs probes exhibited better enhanced MR imaging effects. Combining all these features together, this MRI/NIR fluorescence imaging dual-modal nanoprobes modified with retro-enantio isomers of the peptide have the potential to accurately display GBMs preoperatively for precise imaging and intraoperatively for real-time imaging.

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