scholarly journals Beyond the diffraction limit: far-field fluorescence imaging with ultrahigh resolution

2007 ◽  
Vol 3 (11) ◽  
pp. 781 ◽  
Author(s):  
James H. Rice
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ali Abdolali ◽  
Hooman Barati Sedeh ◽  
Mohammad Hosein Fakheri ◽  
Chen Shen ◽  
Fei Sun

AbstractBased on the transformation acoustics methodology, the design principle for achieving an arbitrary shape magnifying lens (ASML) is proposed. Contrary to the previous works, the presented ASML is competent of realizing far-field high resolution images and breaking the diffraction limit, regardless of the position of the utilized sources. Therefore, objects locating within the designed ASML can be properly resolved in the far-field region. It is shown that the obtained material through the theoretical investigations becomes an acoustic null medium (ANM), which has recently gained a significant attention. Besides the homogeneity of ANM, which makes it an implementable material, it is also independent of the perturbation in the geometry of the lens, in such a way that the same ANM can be used for different structural topologies. The obtained ANM has been implemented via acoustics unit cells formed by membranes and side branches with open ends and then was utilized to realize an ASML with the aid of effective medium theory. It is shown that the far-field results of an ideal ASML abide well with the results of the implemented sample, validating the proposed design principle. The presented acoustic magnifying lens has a wide spectrum of possible applications ranging from medical imaging, and biomedical sensors to focused ultrasound surgery.


2021 ◽  
Vol 3 ◽  
pp. 100067
Author(s):  
Praneeth Gadamsetti ◽  
Markus Testorf ◽  
David A. Gonzalez ◽  
Paolo Batoni ◽  
Menelaos K. Poutous
Keyword(s):  

2021 ◽  
Author(s):  
Michael Weber ◽  
Marcel Leutenegger ◽  
Stefan Stoldt ◽  
Stefan Jakobs ◽  
Tiberiu S. Mihaila ◽  
...  

AbstractWe introduce MINSTED, a fluorophore localization and super-resolution microscopy concept based on stimulated emission depletion (STED) that provides spatial precision and resolution down to the molecular scale. In MINSTED, the intensity minimum of the STED doughnut, and hence the point of minimal STED, serves as a movable reference coordinate for fluorophore localization. As the STED rate, the background and the required number of fluorescence detections are low compared with most other STED microscopy and localization methods, MINSTED entails substantially less fluorophore bleaching. In our implementation, 200–1,000 detections per fluorophore provide a localization precision of 1–3 nm in standard deviation, which in conjunction with independent single fluorophore switching translates to a ~100-fold improvement in far-field microscopy resolution over the diffraction limit. The performance of MINSTED nanoscopy is demonstrated by imaging the distribution of Mic60 proteins in the mitochondrial inner membrane of human cells.


1999 ◽  
Vol 86 (10) ◽  
pp. 5337-5341 ◽  
Author(s):  
A. A. Podshivalov ◽  
M. R. Shepard ◽  
O. I. Matveev ◽  
B. W. Smith ◽  
J. D. Winefordner

2020 ◽  
Vol 38 (8) ◽  
pp. 2322-2327
Author(s):  
Pooria Salami ◽  
Leila Yousefi

Author(s):  
Viktor A. Podolskiy ◽  
Christopher M. Roberts ◽  
Nicolas Olivier ◽  
William P. Wardley ◽  
Bo Fan ◽  
...  

2005 ◽  
Vol 59 (7) ◽  
pp. 868-872 ◽  
Author(s):  
Takeshi Watanabe ◽  
Yoshinori Iketaki ◽  
Takashige Omatsu ◽  
Kimihisa Yamamoto ◽  
Masaaki Fujii

The two-point resolution of a novel two-color far-field super-resolution fluorescence microscopy was evaluated by measuring fluorescent beads 100 nm in diameter. This microscopy is based on a combination of two-color fluorescence dip spectroscopy and a phase-modulation technique for a laser beam. By simply introducing two-color laser light, the size of the fluorescent image of a bead was shrunk down to a diameter of 250 nm from the diffraction-limited image with a diameter of 360 nm. For two closely adjacent fluorescent beads with a separation distance of 350 nm, the two-color microscope clearly gave separated fluorescence images, while the conventional one-color fluorescence microscope could not resolve them. It has been proved that our technique breaks Rayleigh's diffraction limit.


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