scholarly journals High-lose and long-term therapy with interferon-alfa inhibits tumor growth and recurrence in nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential

Hepatology ◽  
2000 ◽  
Vol 32 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Lu Wang ◽  
Zhao-You Tang ◽  
Lun-Xiu Qin ◽  
Xiao-Feng Wu ◽  
Hui-Chuan Sun ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Interferon Alfa ◽  
Human Hepatocellular Carcinoma ◽  
Term Therapy ◽  
High Metastatic Potential ◽  
Long Term Therapy

scholarly journals Hypoxia induces sorafenib resistance mediated by autophagy via activating FOXO3a in hepatocellular carcinoma

2020 ◽  
Vol 11 (11) ◽  
Author(s):  
Chao Liang ◽  
Zhebin Dong ◽  
Xianlei Cai ◽  
Jie Shen ◽  
Yuan Xu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Term Therapy ◽  
Advanced Hepatocellular Carcinoma ◽  
Multikinase Inhibitor ◽  
Sorafenib Treatment ◽  
Clinical Prognosis ◽  
Nuclear Location ◽  
Long Term Therapy ◽  
Hcc Cells

AbstractSorafenib, a multikinase inhibitor, is considered as the only approved drug to cure the advanced hepatocellular carcinoma (HCC); however, the acquired chemoresistance caused by intratumoral hypoxia through sorafenib long term therapy induces sorafenib inefficacy. We demonstrated here that hypoxia significantly attenuated sensitivity of HCC cells to sorafenib treatment and reduced its proliferation. Autophagy was observed in sorafenib-treated HCC cells in hypoxia, and inhibition of autophagy by 3-MA eliminated hypoxia-induced sorafenib resistance. Further study revealed hypoxia-activated FOXO3a, an important cellular stress transcriptional factor, via inducing its dephosphorylation and nuclear location; and FOXO3a-dependent transcriptive activation of beclin-1 was responsible for hypoxia-induced autophagy in HCC cells. Knockout of FOXO3a inhibited the autophagy induced by sorafenib itself in normoxia and significantly enhanced the cytotoxicity of sorafenib in HCC cells; and it also inhibited the hypoxia-induced autophagy and achieved the same effect in sorafenib sensitivity-enhancement in HCC cells as it in normoxia. Finally, knockout of intratumoral FOXO3a significantly enhanced curative efficacy of sorafenib via inhibition of autophagy in xenograft tumors in nude mice. Collectively, our study suggests that FOXO3a plays a key role in regulating hypoxia-induced autophagy in sorafenib-treated HCC, and FOXO3-targeted therapy may serve as a promising approach to improve clinical prognosis of patients suffering from HCC.

scholarly journals Inhibition of Tumor Growth of Human Hepatocellular Carcinoma HepG2 Cells in a Nude Mouse Xenograft Model by the Total Flavonoids fromArachniodes exilis

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Huimin Li ◽  
Dengzhao Jiang ◽  
Lei Zhang ◽  
Jiazhong Wu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Hepg2 Cells ◽  
Xenograft Model ◽  
Human Hepatocellular Carcinoma ◽  
Total Flavonoids ◽  
Tumor Growth Model ◽  
Mouse Xenograft Model

A tumor growth model of human hepatocellular carcinoma HepG2 cells in nude mice was employed to investigate the antitumor activity of the total flavonoids extracted fromArachniodes exilis(TFAE)in vivo. Several biochemical assays including hematoxylin-eosin (HE) staining, immunohistochemistry, and Western blot were performed to elucidate the mechanism of action of total flavonoids extracted fromArachniodes exilis(TFAE). The results showed that TFAE effectively inhibited the tumor growth of hepatocellular carcinoma in nude mice and had no significant effect on body weight, blood system, and functions of liver and kidney. Expression levels of proapoptotic proteins Bax and cleaved caspase-3 remarkably increased while the expressions of Bcl-2, HIF-1α, and VEGF were suppressed by TFAE. These results suggested that the antitumor potential of TFEA was implied by the apoptosis of tumor cells and the inhibition of angiogenesis in tumor tissue.

scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma
Sign in / Sign up

Export Citation Format

Share Document