scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma

scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma

scholarly journals Metastatic human hepatocellular carcinoma models in nude mice and cell line with metastatic potential

2001 ◽  
Vol 7 (5) ◽  
pp. 597 ◽  
Author(s):  
Zhao-You Tang ◽  
Fan-Xian Sun ◽  
Jian Tian ◽  
Sheng-Long Ye ◽  
Yin-Kun Liu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Line ◽  
Nude Mice ◽  
Metastatic Potential ◽  
Human Hepatocellular Carcinoma

scholarly journals Establishment and characteristic of an orthotopic implantation model of human hepatocellular carcinoma with Luc-GFP-labeled in nude mice

2018 ◽  
Author(s):  
Jing-jing Zhang ◽  
Min-hua Xu ◽  
Jie Wang ◽  
Xiao-bao Jin ◽  
Yan Ma
Keyword(s):  
Hepatocellular Carcinoma ◽  
Cell Suspension ◽  
Cell Line ◽  
Nude Mice ◽  
Xenograft Model ◽  
Human Hepatocellular Carcinoma ◽  
Orthotopic Model ◽  
Injection Method ◽  
Tumor Fragment

AbstractAimTo construct Luc-GFP-labeled human hepatocellular carcinoma (HCC) cell line with high metastatic potential. And to establish a spontaneous metastasis and conveniently monitored orthotopic model of hepatocellular carcinoma in nude mice. Methods: HCCLM3-Luc-GFP cell line stably expressing luciferase (Luc) and green fluorescent protein (GFP) was constructed by lentivirus transfection. The orthotopic xenograft model was established though cell suspension injection method and tumor fragment implanted method. The growth and metastasis of the tumors were observed by in vivo imaging and pathology. Results: HCCLM3-Luc-GFP, a highly metastatic HCC cell line with GFP expression and Luc activity, was obtained. The tumorigenic rates both of two approaches were 100%, but the lung metastatic rate was higher the former than the latter. Conclusion: The orthotopic model of highly metastatic and Luc-GFP-labeled HCC in nude mice was successfully established by above approaches, called as cell suspension injection method and tumor fragment implanted method, respectively. This study provides a new and effective means to monitor the growth of tumors in vivo and to evaluate the efficacy of anti-metastatic drugs against HCC.

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