Suppression of Tumor Growth Using Antisense Oligonucleotide Against Survivin in an Orthotopic Transplant Model of Human Hepatocellular Carcinoma in Nude Mice

2006 ◽  
Vol 16 (4) ◽  
pp. 365-374 ◽  
Author(s):  
Yuning Sun ◽  
Ruxian Lin ◽  
Jinming Dai ◽  
Dazhi Jin ◽  
Sheng-Qi Wang
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Antisense Oligonucleotide ◽  
Human Hepatocellular Carcinoma ◽  
Transplant Model

scholarly journals Inhibition of Tumor Growth of Human Hepatocellular Carcinoma HepG2 Cells in a Nude Mouse Xenograft Model by the Total Flavonoids fromArachniodes exilis

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Huimin Li ◽  
Dengzhao Jiang ◽  
Lei Zhang ◽  
Jiazhong Wu
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Hepg2 Cells ◽  
Xenograft Model ◽  
Human Hepatocellular Carcinoma ◽  
Total Flavonoids ◽  
Tumor Growth Model ◽  
Mouse Xenograft Model

A tumor growth model of human hepatocellular carcinoma HepG2 cells in nude mice was employed to investigate the antitumor activity of the total flavonoids extracted fromArachniodes exilis(TFAE)in vivo. Several biochemical assays including hematoxylin-eosin (HE) staining, immunohistochemistry, and Western blot were performed to elucidate the mechanism of action of total flavonoids extracted fromArachniodes exilis(TFAE). The results showed that TFAE effectively inhibited the tumor growth of hepatocellular carcinoma in nude mice and had no significant effect on body weight, blood system, and functions of liver and kidney. Expression levels of proapoptotic proteins Bax and cleaved caspase-3 remarkably increased while the expressions of Bcl-2, HIF-1α, and VEGF were suppressed by TFAE. These results suggested that the antitumor potential of TFEA was implied by the apoptosis of tumor cells and the inhibition of angiogenesis in tumor tissue.

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