Inhibitory effect of the angiogenesis inhibitor TNP-470 on tumor growth and metastasis in nude mice bearing human hepatocellular carcinoma

1997 ◽  
Vol 123 (7) ◽  
pp. 383-387 ◽  
Author(s):  
Jing-Lin Xia ◽  
Bing-Hui Yang ◽  
Zhao-You Tang ◽  
Fang-Xian Sun ◽  
Qiong Xue ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Angiogenesis Inhibitor ◽  
Inhibitory Effect ◽  
Human Hepatocellular Carcinoma ◽  
Tumor Growth And Metastasis

scholarly journals Isomalto oligosaccharide sulfate inhibits tumor growth and metastasis of hepatocellular carcinoma in nude mice

BMC Cancer ◽  
2011 ◽  
Vol 11 (1) ◽  
Author(s):  
Chun-Li Xiao ◽  
Zhong-Hua Tao ◽  
Lin Guo ◽  
Wei-Wei Li ◽  
Jin-Liang Wan ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Tumor Growth ◽  
Nude Mice ◽  
Tumor Growth And Metastasis

scholarly journals Inhibitory Effect of Endostar on Specific Angiogenesis Induced by Human Hepatocellular Carcinoma

2015 ◽  
Vol 2015 ◽  
pp. 1-9 ◽  
Author(s):  
Qing Ye ◽  
Shukui Qin ◽  
Yanhong Liu ◽  
Jundong Feng ◽  
Qiong Wu ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Angiogenesis Inhibitor ◽  
Inhibitory Effect ◽  
Tube Formation ◽  
Human Hepatocellular Carcinoma ◽  
Conditioned Media ◽  
Dependent Manner ◽  
Matrigel Plug ◽  
Dose Dependent

To investigate the effect of endostar on specific angiogenesis induced by human hepatocellular carcinoma, this research systematically elucidated the inhibitory effect on HepG2-induced angiogenesis by endostar from 50 ng/mL to 50000 ng/mL. We employed fluorescence quantitative Boyden chamber analysis, wound-healing assay, flow cytometry examination using a coculture system, quantitative analysis of tube formation, andin vivoMatrigel plug assay induced by HCC conditioned media (HCM) and HepG2 compared with normal hepatocyte conditioned media (NCM) and L02. Then, we found that endostar as a tumor angiogenesis inhibitor could potently inhibit human umbilical vein endothelial cell (HUVEC) migration in response to HCM after four- to six-hour action, inhibit HCM-induced HUVEC migration to the lesion part in a dose-dependent manner between 50 ng/mL and 5000 ng/mL at 24 hours, and reduce HUVEC proliferation in a dose-dependent fashion. Endostar inhibited HepG2-induced tube formation of HUVECs which peaked at 50 ng/mL.In vivoMatrigel plug formation was also significantly reduced by endostar in HepG2 inducing system rather than in L02 inducing system. It could be concluded that, at cell level, endostar inhibited the angiogenesis-related biological behaviors of HUVEC in response to HCC, including migration, adhesion proliferation, and tube formation. At animal level, endostar inhibited the angiogenesis in response to HCC in Matrigel matrix.

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