scholarly journals Solubility–insolubility interconversion of sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, regulated by the sugar-specific interaction

2004 ◽  
Vol 382 (3) ◽  
pp. 821-829 ◽  
Author(s):  
Haruko UEDA ◽  
Hisako FUKUSHIMA ◽  
Yasumaru HATANAKA ◽  
Haruko OGAWA
Keyword(s):  
Specific Interaction ◽  
Sugar Concentration ◽  
Tree Bark ◽  
Specific Interactions ◽  
Sophora Japonica ◽  
Legume Lectin ◽  
Insoluble Polymer ◽  
Parenchymal Cells ◽  
Self Aggregation ◽  
Soluble Complexes

Sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, was the first lectin found to show self-aggregation that is dependent on the sugar concentration accompanying the interconversion between solubility and insolubility [Ueno, Ogawa, Matsumoto and Seno (1991) J. Biol. Chem. 266, 3146–3153]. The interconversion is regulated by the concentrations of Ca2+ and specific sugars: mannose, glucose or sucrose. The specific glycotopes for sophoragrin were found in the sophoragrin subunit and an endogenous galactose-specific lectin, B-SJA-I (bark S. japonica agglutinin I), and the lectin subunit that binds to the glycotope was identified by photoaffinity glycan probes. Remarkably, the insoluble polymer of sophoragrin is dissociated by interaction with B-SJA-I into various soluble complexes. Based on these results, self-aggregation of sophoragrin was shown to be a unique homopolymerization due to the sugar-specific interaction. An immunostaining study indicated that sophoragrin localizes mainly in vacuoles of parenchymal cells coincidently with B-SJA-I. These results indicate that sophoragrin can sequester endogenous glycoprotein ligands via sugar-specific interactions, thus providing new insights into the occurrence and significance of the intravacuolar interaction shown by a legume lectin.

Precisely controlled molecular imprinting of glutathione-s-transferase by orientated template immobilization using specific interaction with an anchored ligand on a gold substrate

2014 ◽  
Vol 5 (16) ◽  
pp. 4764-4771 ◽  
Author(s):  
Yuri Kamon ◽  
Ryo Matsuura ◽  
Yukiya Kitayama ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Thin Films ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymer ◽  
Specific Interaction ◽  
Specific Interactions ◽  
Synthetic Route ◽  
Gold Substrate ◽  
Imprinted Polymer ◽  
Glutathione S Transferase ◽  
Molecularly Imprinted

We demonstrate a novel synthetic route for molecularly imprinted polymer (MIP) thin films using a bottom-up approach utilizing protein–ligand specific interactions.

Carbohydrate-Carbohydrate Recognition Promotes Cell Adhesion

2005 ◽  
Vol 872 ◽  
Author(s):  
Christine Gourier ◽  
Eric Perez ◽  
Yongmin Zhang ◽  
Pierre Sinaÿ
Keyword(s):  
Cell Adhesion ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Second Step ◽  
Specific Interactions ◽  
Carbohydrate Recognition ◽  
Giant Vesicles ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
New Type

AbstractRecently, carbohydate-carbohydrate recognition has emerged as a new type of interaction in cell adhesion processes. One of these carbohydrates, the LewisX determinant (LeX), has been shown to be involved in murine embryogenesis. Here we confirm the existence of this specific interaction by measuring the adhesion between giant vesicles functionalised with synthetic Lex bearing lipids providing to the LeXdeterminent a high orientational freedom. This was obtained by micropipette aspiration and contact angle measurements. By using a simple model involving the several contributions to the adhesion free energy, specific and non specific interactions could be separated and quantified. In a second step, using natural Lex bearing sphingolipids, we could underscore the high sensitivity of LeX-LeX recognition to molecular structure and prove that the possible orientations provided by the natural LeX bearing molecules not only allow but strongly favor LeX-LeX recognition.

scholarly journals Non-Specific and Specific Interactions of Hydrocarbons with the Surface of a Non-Swelling Organo-Substituted Layer Silicate

1989 ◽  
Vol 6 (3) ◽  
pp. 147-154 ◽  
Author(s):  
Yu.I. Tarasevich ◽  
S.V. Bondarenko ◽  
Yu.V. Shulepov ◽  
E.V. Aksenenko
Keyword(s):  
Statistical Method ◽  
Specific Interaction ◽  
Benzene Molecule ◽  
Adsorption Complex ◽  
Specific Interactions ◽  
Layer Silicate ◽  
Energy Characteristics ◽  
Kaolinite Surface

A molecular statistical method has been used to describe both specific and non-specific sorption of hydrocarbons on the surface of a non-swelling layer silicate (kaolinite) modified with octadecylammonium chloride. Sorption of n-hexane on this surface appears to be determined primarily by dispersion attraction to the modified overlayer, while specific interaction has been shown to play a major role in the case of benzene sorption. The energy characteristics of the benzene—RNH3+ adsorption complex have been estimated and the assumption that the benzene molecule is partially delocalized on the modified kaolinite surface shown to be reasonable.

scholarly journals Division of labour between different PP2A-B56 complexes during mitosis

2018 ◽  
Author(s):  
Giulia Vallardi ◽  
Lindsey A Allan ◽  
Lisa Crozier ◽  
Adrian T Saurin
Keyword(s):  
Sister Chromatid ◽  
Critical Region ◽  
Spindle Assembly Checkpoint ◽  
Specific Interaction ◽  
Division Of Labour ◽  
Specific Interactions ◽  
Microtubule Attachment ◽  
Chromatid Cohesion ◽  
Terminal Loop ◽  
Selective Interactions

PP2A-B56 is a serine/threonine phosphatase complex that regulates several major mitotic processes, including sister chromatid cohesion, kinetochore-microtubule attachment and the spindle assembly checkpoint. We show here that these key functions are divided between B56 isoforms that localise differentially to either the centromere or kinetochore. The centromeric B56 isoforms rely on a specific interaction with Sgo2, whereas the kinetochore isoforms bind preferentially to BubR1 and other proteins containing an LxxIxE motif. In addition to these selective interactions, Sgo1 also contributes to both localisations by collaborating with BubR1 to maintain B56 isoforms at the kinetochore and helping to anchor the Sgo2/B56 complex at the centromere. A series of chimaeras were used to map the critical region in B56 to a small C-terminal loop that specifies which interactions are favoured and therefore defines where B56 isoforms localise during prometaphase. Together, this study describes how different PP2A-B56 complexes utilise isoform-specific interactions to control distinct processes during mitosis.

Gas-liquid critical temperatures of mixtures containing electron donors

1977 ◽  
Vol 30 (2) ◽  
pp. 401 ◽  
Author(s):  
GJ Campbell ◽  
RL Hurle ◽  
SP Lie ◽  
CL Young
Keyword(s):  
Interaction Energy ◽  
Diethyl Ether ◽  
Thermodynamic Data ◽  
Fluid Model ◽  
Specific Interaction ◽  
Energy Parameter ◽  
Electron Donors ◽  
The Other ◽  
Specific Interactions ◽  
Critical Temperatures

The gas-liquid critical temperatures, Tcm, of some mixtures of the electron donors, triethylamine, diethyl ether and diisopropyl ether with n-alkanes, benzene and hexafluorobenzene are reported. By using the van der Waals one-fluid model, an interaction energy parameter, ξ, has been calculated for each mixture from the values of T°m. The values of ξ for the n-alkane+electron donors are fairly close to unity, indicating that, as would be expected, there are no strong specific interactions between the unlike molecules. The values of ( for the electron donors with benzene give no definite indication of specific interactions. On the other hand, values of ξ for the electron donors with hexafluorobenzene indicate a specific interaction between the unlike molecules. These conclusions are discussed in relation to those reached from a consideration of other thermodynamic data.

scholarly journals Informed movement and dispersal in experimental metacommunities

2015 ◽  
Author(s):  
Emanuel A. Fronhofer ◽  
Jan Klecka ◽  
Carlos J. Melián ◽  
Florian Altermatt
Keyword(s):  
Interspecific Interactions ◽  
Specific Interaction ◽  
Interaction Strength ◽  
Information Use ◽  
Single Species ◽  
Specific Interactions ◽  
Movement Behaviour ◽  
Behavioural Responses ◽  
Metacommunity Dynamics ◽  
Dynamics Stability

Dispersal, and the underlying movement behaviour, are processes of pivotal importance for understanding and predicting metapopulation and metacommunity dynamics. Generally, dispersal decisions are non-random and rely on information, such as the presence of conspecifics. However, studies on metacommunities that include interspecific interactions generally disregard information use. Therefore, it remains unclear whether and how dispersal in metacommunities is informed and whether rules derived from single-species contexts can be scaled up to (meta-)communities. Using experimental protist metacommunities, we show how dispersal and movement are informed and adjusted by the strength of inter-specific interactions. We found that predicting informed movement and dispersal in metacommunities requires knowledge on behavioural responses to intra- and inter-specific interaction strength. Consequently, metacommunity dynamics inferred directly from single-species metapopulations without taking inter-specific interactions into account are likely flawed. Our work identifies the significance of information use for understanding metacommunity dynamics, stability and the coexistence and distribution of species.

Activity coefficient assumptions in the determination of association constants. V. Association of thallium(I) bromide from solubility measurements

1969 ◽  
Vol 22 (5) ◽  
pp. 911 ◽  
Author(s):  
CW Childs ◽  
MH Panckhurst
Keyword(s):  
Activity Coefficient ◽  
Sodium Acetate ◽  
Specific Interaction ◽  
Sodium Perchlorate ◽  
Potassium Bromide ◽  
Association Constants ◽  
Sodium Bromide ◽  
Specific Interactions ◽  
Free Ions

Measurements are reported of the solubility of thallium(I) bromide in aqueous solutions of potassium bromide, sodium perchlorate, barium bromide, and of mixtures of sodium bromide and sodium perchlorate and of sodium bromide and sodium acetate at 25�. The measurements are used to test the merits of two types of ionic activity coefficient expressions, one assuming specific interactions between free ions and the other not. It is shown that neither expression can describe our results adequately, in contrast to previous solubility results with thallium(I) chloride for which the specific interaction expression was adequate.

scholarly journals A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2

2019 ◽  
Vol 10 (6) ◽  
pp. 1579-1588 ◽  
Author(s):  
Fangyuan Gao ◽  
Fenglong Jiao ◽  
Chaoshuang Xia ◽  
Yang Zhao ◽  
Wantao Ying ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Specific Interaction ◽  
Phospholipid Bilayers ◽  
Phospholipid Bilayer ◽  
Specific Interactions ◽  
Short Period ◽  
Novel Strategy ◽  
Phosphate Groups

Exosomes can be efficiently isolated in a short period of time by the specific interaction of titanium dioxide with the phosphate groups on the surface of phospholipid bilayer.

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