scholarly journals A novel strategy for facile serum exosome isolation based on specific interactions between phospholipid bilayers and TiO2

2019 ◽  
Vol 10 (6) ◽  
pp. 1579-1588 ◽  
Author(s):  
Fangyuan Gao ◽  
Fenglong Jiao ◽  
Chaoshuang Xia ◽  
Yang Zhao ◽  
Wantao Ying ◽  
...  
Keyword(s):  
Titanium Dioxide ◽  
Specific Interaction ◽  
Phospholipid Bilayers ◽  
Phospholipid Bilayer ◽  
Specific Interactions ◽  
Short Period ◽  
Novel Strategy ◽  
Phosphate Groups

Exosomes can be efficiently isolated in a short period of time by the specific interaction of titanium dioxide with the phosphate groups on the surface of phospholipid bilayer.

Asymmetric Distribution of Phosphatidyl Serine in Supported Phospholipid Bilayers on Titanium Dioxide

Langmuir ◽  
2006 ◽  
Vol 22 (8) ◽  
pp. 3467-3473 ◽  
Author(s):  
Fernanda F. Rossetti ◽  
Marcus Textor ◽  
Ilya Reviakine
Keyword(s):  
Titanium Dioxide ◽  
Phosphatidyl Serine ◽  
Phospholipid Bilayers ◽  
Asymmetric Distribution

Precisely controlled molecular imprinting of glutathione-s-transferase by orientated template immobilization using specific interaction with an anchored ligand on a gold substrate

2014 ◽  
Vol 5 (16) ◽  
pp. 4764-4771 ◽  
Author(s):  
Yuri Kamon ◽  
Ryo Matsuura ◽  
Yukiya Kitayama ◽  
Tooru Ooya ◽  
Toshifumi Takeuchi
Keyword(s):  
Thin Films ◽  
Molecular Imprinting ◽  
Molecularly Imprinted Polymer ◽  
Specific Interaction ◽  
Specific Interactions ◽  
Synthetic Route ◽  
Gold Substrate ◽  
Imprinted Polymer ◽  
Glutathione S Transferase ◽  
Molecularly Imprinted

We demonstrate a novel synthetic route for molecularly imprinted polymer (MIP) thin films using a bottom-up approach utilizing protein–ligand specific interactions.

Carbohydrate-Carbohydrate Recognition Promotes Cell Adhesion

2005 ◽  
Vol 872 ◽  
Author(s):  
Christine Gourier ◽  
Eric Perez ◽  
Yongmin Zhang ◽  
Pierre Sinaÿ
Keyword(s):  
Cell Adhesion ◽  
Specific Interaction ◽  
High Sensitivity ◽  
Second Step ◽  
Specific Interactions ◽  
Carbohydrate Recognition ◽  
Giant Vesicles ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
New Type

AbstractRecently, carbohydate-carbohydrate recognition has emerged as a new type of interaction in cell adhesion processes. One of these carbohydrates, the LewisX determinant (LeX), has been shown to be involved in murine embryogenesis. Here we confirm the existence of this specific interaction by measuring the adhesion between giant vesicles functionalised with synthetic Lex bearing lipids providing to the LeXdeterminent a high orientational freedom. This was obtained by micropipette aspiration and contact angle measurements. By using a simple model involving the several contributions to the adhesion free energy, specific and non specific interactions could be separated and quantified. In a second step, using natural Lex bearing sphingolipids, we could underscore the high sensitivity of LeX-LeX recognition to molecular structure and prove that the possible orientations provided by the natural LeX bearing molecules not only allow but strongly favor LeX-LeX recognition.

scholarly journals Non-Specific and Specific Interactions of Hydrocarbons with the Surface of a Non-Swelling Organo-Substituted Layer Silicate

1989 ◽  
Vol 6 (3) ◽  
pp. 147-154 ◽  
Author(s):  
Yu.I. Tarasevich ◽  
S.V. Bondarenko ◽  
Yu.V. Shulepov ◽  
E.V. Aksenenko
Keyword(s):  
Statistical Method ◽  
Specific Interaction ◽  
Benzene Molecule ◽  
Adsorption Complex ◽  
Specific Interactions ◽  
Layer Silicate ◽  
Energy Characteristics ◽  
Kaolinite Surface

A molecular statistical method has been used to describe both specific and non-specific sorption of hydrocarbons on the surface of a non-swelling layer silicate (kaolinite) modified with octadecylammonium chloride. Sorption of n-hexane on this surface appears to be determined primarily by dispersion attraction to the modified overlayer, while specific interaction has been shown to play a major role in the case of benzene sorption. The energy characteristics of the benzene—RNH3+ adsorption complex have been estimated and the assumption that the benzene molecule is partially delocalized on the modified kaolinite surface shown to be reasonable.

scholarly journals Solubility–insolubility interconversion of sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, regulated by the sugar-specific interaction

2004 ◽  
Vol 382 (3) ◽  
pp. 821-829 ◽  
Author(s):  
Haruko UEDA ◽  
Hisako FUKUSHIMA ◽  
Yasumaru HATANAKA ◽  
Haruko OGAWA
Keyword(s):  
Specific Interaction ◽  
Sugar Concentration ◽  
Tree Bark ◽  
Specific Interactions ◽  
Sophora Japonica ◽  
Legume Lectin ◽  
Insoluble Polymer ◽  
Parenchymal Cells ◽  
Self Aggregation ◽  
Soluble Complexes

Sophoragrin, a mannose/glucose-specific lectin in Sophora japonica (Japanese pagoda tree) bark, was the first lectin found to show self-aggregation that is dependent on the sugar concentration accompanying the interconversion between solubility and insolubility [Ueno, Ogawa, Matsumoto and Seno (1991) J. Biol. Chem. 266, 3146–3153]. The interconversion is regulated by the concentrations of Ca2+ and specific sugars: mannose, glucose or sucrose. The specific glycotopes for sophoragrin were found in the sophoragrin subunit and an endogenous galactose-specific lectin, B-SJA-I (bark S. japonica agglutinin I), and the lectin subunit that binds to the glycotope was identified by photoaffinity glycan probes. Remarkably, the insoluble polymer of sophoragrin is dissociated by interaction with B-SJA-I into various soluble complexes. Based on these results, self-aggregation of sophoragrin was shown to be a unique homopolymerization due to the sugar-specific interaction. An immunostaining study indicated that sophoragrin localizes mainly in vacuoles of parenchymal cells coincidently with B-SJA-I. These results indicate that sophoragrin can sequester endogenous glycoprotein ligands via sugar-specific interactions, thus providing new insights into the occurrence and significance of the intravacuolar interaction shown by a legume lectin.

scholarly journals Enzyme-mediated single-nucleotide variation detection at room temperature with high discrimination factor

2015 ◽  
Vol 6 (2) ◽  
pp. 1206-1211 ◽  
Author(s):  
Tongbo Wu ◽  
Xianjin Xiao ◽  
Zhe Zhang ◽  
Meiping Zhao
Keyword(s):  
Room Temperature ◽  
Specific Interaction ◽  
Nucleotide Variation ◽  
Single Nucleotide Variation ◽  
Lambda Exonuclease ◽  
Single Nucleotide ◽  
Chemically Modified ◽  
Modified Dna ◽  
High Discrimination ◽  
Novel Strategy

We present a novel strategy for the highly selective detection of single-nucleotide variation at room temperature, based on an extremely specific interaction between Lambda exonuclease (λ exo) and a chemically modified DNA structure.

scholarly journals Division of labour between different PP2A-B56 complexes during mitosis

2018 ◽  
Author(s):  
Giulia Vallardi ◽  
Lindsey A Allan ◽  
Lisa Crozier ◽  
Adrian T Saurin
Keyword(s):  
Sister Chromatid ◽  
Critical Region ◽  
Spindle Assembly Checkpoint ◽  
Specific Interaction ◽  
Division Of Labour ◽  
Specific Interactions ◽  
Microtubule Attachment ◽  
Chromatid Cohesion ◽  
Terminal Loop ◽  
Selective Interactions

PP2A-B56 is a serine/threonine phosphatase complex that regulates several major mitotic processes, including sister chromatid cohesion, kinetochore-microtubule attachment and the spindle assembly checkpoint. We show here that these key functions are divided between B56 isoforms that localise differentially to either the centromere or kinetochore. The centromeric B56 isoforms rely on a specific interaction with Sgo2, whereas the kinetochore isoforms bind preferentially to BubR1 and other proteins containing an LxxIxE motif. In addition to these selective interactions, Sgo1 also contributes to both localisations by collaborating with BubR1 to maintain B56 isoforms at the kinetochore and helping to anchor the Sgo2/B56 complex at the centromere. A series of chimaeras were used to map the critical region in B56 to a small C-terminal loop that specifies which interactions are favoured and therefore defines where B56 isoforms localise during prometaphase. Together, this study describes how different PP2A-B56 complexes utilise isoform-specific interactions to control distinct processes during mitosis.

Differential effect of arginine modification with 1,2-cyclohexanedione on the capacity of vimentin and desmin to assemble into intermediate filaments and to bind to nucleic acids

1984 ◽  
Vol 65 (1) ◽  
pp. 1-20
Author(s):  
P. Traub ◽  
C.E. Vorgias
Keyword(s):  
Nucleic Acids ◽  
Intermediate Filaments ◽  
Intermediate Filament ◽  
Specific Interaction ◽  
Inhibitory Effect ◽  
Affinity Column ◽  
Intermediate Filament Proteins ◽  
Amino Terminal ◽  
Short Period ◽  
Arginine Residues

When the intermediate filament proteins vimentin and desmin were reacted for a short period of time with the arginine-specific reagent 1,2-cyclohexanedione, the modification had a severe, inhibitory effect on the assembly of intermediate filaments and on the susceptibility of the basic, amino-terminal polypeptide of both proteins to degradation by the intermediate filament-specific, Ca2+-activated proteinase. However, it had only a slightly inhibitory effect on the binding of vimentin and desmin to ribosomal RNA from Ehrlich ascites tumour cells. Since the Ca2+-activated proteinase is very likely to be a trypsin-like enzyme, with a preference for arginyl and lysyl peptide bonds, the results indicate that the arginine residues of the amino-terminal polypeptide of vimentin and desmin are highly essential for filament assembly but largely dispensable for the binding of both proteins to nucleic acids. This was supported by the observation that two breakdown products of vimentin lacking a 5 X 10(3) Mr and an 8 X 10(3) Mr polypeptide from the amino terminus, respectively, did not assemble into intermediate filaments but were still capable of binding to rRNA. Both polypeptides also bound to single-stranded DNA-cellulose under non-denaturing conditions, but passed the affinity column in the presence of 6 M-urea. Thus, the binding of vimentin to nucleic acids appears to be based on two components: a non-specific electrostatic interaction mediated by the positively charged arginine residues of the amino-terminal polypeptide that is insensitive to denaturation by urea, and a specific interaction that is sensitive to denaturation by urea.

Gas-liquid critical temperatures of mixtures containing electron donors

1977 ◽  
Vol 30 (2) ◽  
pp. 401 ◽  
Author(s):  
GJ Campbell ◽  
RL Hurle ◽  
SP Lie ◽  
CL Young
Keyword(s):  
Interaction Energy ◽  
Diethyl Ether ◽  
Thermodynamic Data ◽  
Fluid Model ◽  
Specific Interaction ◽  
Energy Parameter ◽  
Electron Donors ◽  
The Other ◽  
Specific Interactions ◽  
Critical Temperatures

The gas-liquid critical temperatures, Tcm, of some mixtures of the electron donors, triethylamine, diethyl ether and diisopropyl ether with n-alkanes, benzene and hexafluorobenzene are reported. By using the van der Waals one-fluid model, an interaction energy parameter, ξ, has been calculated for each mixture from the values of T°m. The values of ξ for the n-alkane+electron donors are fairly close to unity, indicating that, as would be expected, there are no strong specific interactions between the unlike molecules. The values of ( for the electron donors with benzene give no definite indication of specific interactions. On the other hand, values of ξ for the electron donors with hexafluorobenzene indicate a specific interaction between the unlike molecules. These conclusions are discussed in relation to those reached from a consideration of other thermodynamic data.

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