A catechol-chitosan-based adhesive and injectable hydrogel resistant to oxidation and compatible with cell therapy

2021 ◽  
Author(s):  
Capucine Guyot ◽  
Atma Adoungotchodo ◽  
Werner Taillades ◽  
Marta Cerruti ◽  
Sophie Lerouge
Keyword(s):  
Cell Therapy ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Encapsulated Cells

Injectable hydrogels designed for cell therapy need to be adhesive to the surrounding tissues to maximize their retention and the communication between the host and the encapsulated cells. Catechol grafting...

Physically crosslinked injectable hydrogels for long-term delivery of oncolytic adenoviruses for cancer treatment

2019 ◽  
Vol 7 (10) ◽  
pp. 4195-4207 ◽  
Author(s):  
Thai Minh Duy Le ◽  
Bo-Kyeong Jung ◽  
Yi Li ◽  
Huu Thuy Trang Duong ◽  
Thanh Loc Nguyen ◽  
...  
Keyword(s):  
Cancer Treatment ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Temperature Responsive ◽  
Oncolytic Adenoviruses ◽  
Term Delivery ◽  
Physically Crosslinked ◽  
Hydrogel System

A dual pH- and temperature-responsive physically crosslinked and injectable hydrogel system was developed for efficient and long-term delivery of oncolytic adenoviruses (Ads).

Strong underwater adhesion of injectable hydrogels triggered by diffusion of small molecules

2021 ◽  
Author(s):  
Xing Su ◽  
Wenyue Xie ◽  
Pudi Wang ◽  
Zhuoling Tian ◽  
Hao Wang ◽  
...  
Keyword(s):  
Small Molecules ◽  
Tea Polyphenols ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Underwater Adhesion ◽  
Physically Crosslinked

It is challenging for injectable hydrogels to achieve high underwater adhesiveness. Based on this concern, we report a fully physically crosslinked injectable hydrogel composed of gelatin, tea polyphenols and urea,...

Immunoisolated Xenogeneic Chromaffin Cell Therapy for Chronic Pain

1996 ◽  
Vol 85 (5) ◽  
pp. 1005-1012 ◽  
Author(s):  
E. Buchser ◽  
M. Goddard ◽  
B. Heyd ◽  
J. M. Joseph ◽  
J. Favre ◽  
...  
Keyword(s):  
Chronic Pain ◽  
Cell Therapy ◽  
Chromaffin Cells ◽  
Double Blind ◽  
Encapsulated Cells ◽  
Human Scale ◽  
Potential Efficacy ◽  
Biologic Effects ◽  
Pain Ratings ◽  
Clinical Potential

Background Chromaffin cells from the adrenal gland secrete a mixture of compounds that have a strong analgesic effect, especially when administered intrathecally. Many studies in animal models have shown that discordant xenogeneic cell isolates, including chromaffin cells, can survive and have biologic effects when transplanted within a semipermeable membrane capsule. Methods To evaluate the clinical potential of encapsulated cell therapy, a human-scale implant containing bovine chromaffin cells was developed, characterized, and implanted in the subarachnoid space of seven patients with severe chronic pain not satisfactorily managed with conventional therapies. Patients received no pharmacologic immunosuppression. Cell devices were implanted during minimally invasive surgery, and device design allowed retrieval. All devices were recovered after implant periods of 41 to 176 days. Results Postexplant histologic analysis, immunostaining, and secretory function all confirmed survival and biochemical function of the encapsulated cells. Reductions in morphine intake and improvement in pain ratings were observed in several patients. Conclusions This study represents the first successful trial of encapsulated xenogeneic cells in humans. The preliminary findings of pain reduction warrant the initiation of a randomized, double-blind phase II study to evaluate the potential efficacy of the procedure.

scholarly journals A Low-Cost Open Source Device for Cell Microencapsulation

Materials ◽  
2020 ◽  
Vol 13 (22) ◽  
pp. 5090
Author(s):  
Miriam Salles Pereira ◽  
Liana Monteiro da Fonseca Cardoso ◽  
Tatiane Barreto da Silva ◽  
Ayla Josma Teixeira ◽  
Saul Eliahú Mizrahi ◽  
...  
Keyword(s):  
Immune System ◽  
Cell Therapy ◽  
Open Source ◽  
Low Cost ◽  
Cell Encapsulation ◽  
Host Cells ◽  
Host Immune System ◽  
Middle Income ◽  
Encapsulated Cells ◽  
Cell Microencapsulation

Microencapsulation is a widely studied cell therapy and tissue bioengineering technique, since it is capable of creating an immune-privileged site, protecting encapsulated cells from the host immune system. Several polymers have been tested, but sodium alginate is in widespread use for cell encapsulation applications, due to its low toxicity and easy manipulation. Different cell encapsulation methods have been described in the literature using pressure differences or electrostatic changes with high cost commercial devices (about 30,000 US dollars). Herein, a low-cost device (about 100 US dollars) that can be created by commercial syringes or 3D printer devices has been developed. The capsules, whose diameter is around 500 µm and can decrease or increase according to the pressure applied to the system, is able to maintain cells viable and functional. The hydrogel porosity of the capsule indicates that the immune system is not capable of destroying host cells, demonstrating that new studies can be developed for cell therapy at low cost with microencapsulation production. This device may aid pre-clinical and clinical projects in low- and middle-income countries and is lined up with open source equipment devices.

scholarly journals Recent Advances in Injectable Hydrogels for Biomedical Applications

2019 ◽  
Vol 2 (1) ◽  
pp. 1 ◽  
Author(s):  
Eko Adi Prasetyanto
Keyword(s):  
Tissue Engineering ◽  
Biomedical Applications ◽  
State Of The Art ◽  
Research Progress ◽  
Hybrid Hydrogel ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Different Types ◽  
And Cartilage ◽  
Recent Research Progress

Injectable hydrogels, a class of hydrogel, have received a lot of attention in biomedical applications due to its versatility. It is reported that injectable hydrogel can be applied in various biomedical procedures for example as submucosal fluid cushion, periodontal implant, and cartilage and bone tissue engineering. In addition to its easy delivery (implantation), this class of hydrogel can be tailored to match specific applications. The customization of this hydrogel can be easily executed by changing polymeric backbone of hydrogel, choosing different types of crosslinking or by adding nanoparticles to form hybrid hydrogel systems. Physical properties, compatibility and biodegradability of the resulted materials are important factors for designing injectable hydrogels. In this Recent Research Progress, we highlight the state-of-the-art injectable hydrogels and note the general requirements of an ideal injectable hydrogel for biomedical applications.

scholarly journals Mechanisms of ischemic skeletal muscle regeneration mediated by mechanically constrained human allogeneic mesenchymal stromal cells

2020 ◽  
Vol 3 ◽  
Author(s):  
Alex O'Connor ◽  
Steven Miller ◽  
Michael Loke ◽  
Chang-Hyun Gil ◽  
Katherin Leckie ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Cell Therapy ◽  
Muscle Regeneration ◽  
Significant Risk ◽  
Skeletal Muscle Regeneration ◽  
Mouse Tissue ◽  
Diabetic Patients ◽  
Tissue Samples ◽  
Encapsulated Cells ◽  
Starting Point

Background and Hypothesis: Critical limb threatening ischemia (CLTI) is the end stage of peripheral arterial disease (PAD). CLTI presents a significant risk for lower extremity amputation, especially in diabetic patients with poor options for revascularization. Additionally, using a novel diabetic mouse model of CLTI, we have shown that IM injection of 3D cultured MSCs (spheroids) is more effective in promoting skeletal muscle regeneration of ischemic muscle than monolayer cultured MSC. We hypothesize that this result is due to the mechanical constrained nature of the MSC in spheroid form, which has been shown to alter the cellular phenotype. Alginate encapsulated cells are another form of mechanical confinement, and have additional benefits including resistance to immunological attack, making them a practical therapy for treatment of CLTI patients.    Project Methods: In this work, cells were encapsulated in 2% alginate using a centrifugation method. Media from these cells along with others were analyzed for IL-10 and IL-33 using ELISA. Mouse tissue samples were stained with WGA-555 antibody and analyzed using a scanning microscope. Effects on tissue perfusion were measured with Laser Doppler Perfusion Imaging. At the time of this abstract, the media from encapsulated and naked MSCs is being used to culture myoblasts, looking at cell growth.    Results: ELISA results did not show significant increases in IL-10 or IL-33 for encapsulated vs. non-encapsulated cells. LDPI has shown an increased perfusion rate for hindlimbs treated with encapsulated MSCs vs naked MSCs. Muscle fiber analysis is ongoing, but initial data appears promising.     Conclusion and Potential Impact: This experiment provides a starting point for improving and expanding cell therapy for critical limb ischemia, potentially resulting in better outcomes for diabetic patients and preventing lower limb amputations. The encapsulation process also has value in other types of cell therapy, as it could protect cells from host defenses and increase dwell time. 

Cell therapy using encapsulated cells producing endostatin

2003 ◽  
pp. 137-141 ◽  
Author(s):  
R. Bjerkvig ◽  
T.-A. Read ◽  
P. Vajkoczy ◽  
P. Aebischer ◽  
W. Pralong ◽  
...  
Keyword(s):  
Cell Therapy ◽  
Encapsulated Cells

scholarly journals Injectable hydrogels self-assembled from oligopeptide-poly(2-methacryloyloxyethyl phosphorylcholine) hybrid graft copolymers for cell scaffolds and controlled release applications

2021 ◽  
Author(s):  
Tomoyuki Koga ◽  
Tomoo Matsuoka ◽  
Yusuke Morita ◽  
Nobuyuki Higashi
Keyword(s):  
Controlled Release ◽  
Graft Copolymers ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel ◽  
Self Assembling ◽  
Cell Scaffolds ◽  
Self Assembled

An injectable hydrogel composed of biocompatible PMPC with PEG-attached self-assembling peptide grafts was newly prepared, and it could be used as 3D cell scaffolds and controlled-release applications.

scholarly journals Regeneration of Articular Cartilage Using Injectable Hybrid Polymer Hydrogel Incorporated With Nanoparticles

2021 ◽  
pp. 394-397
Author(s):  
T. Ruhina ◽  
N. Mahdhia Begum ◽  
S. Padmavathi ◽  
I. Sahanajith ◽  
K. Karthick Babu
Keyword(s):  
Articular Cartilage ◽  
Cartilage Repair ◽  
Cartilage Damage ◽  
Cartilage Tissue Engineering ◽  
Treatment Strategies ◽  
Bioactive Molecules ◽  
Cartilage Tissue ◽  
Human Beings ◽  
Injectable Hydrogels ◽  
Injectable Hydrogel

Cartilage damage is still a threat to human beings, yet there is currently no treatment available to fully restore the function of cartilage. Recently, due to their unique structures and properties, injectable hydrogels have been widely studied and have exhibited high potential for applications in therapeutic areas, especially in cartilage repair. In this review, briefly introduce the properties of cartilage, some articular cartilage injuries, and now available treatment strategies. Afterwards,the functional and fundamental requirements of injectable hydrogels in cartilage tissue engineering, as well as the main advantages of injectable hydrogels as a therapy for cartilage damage, including strong plasticity and excellent biocompatibility. Moreover, we comprehensively summarize the polymers, cells, and bioactive molecules regularly used in the fabrication of injectable hydrogel, with two kinds of gelation, i.e., physical and chemical crosslinking, which ensure the excellence design of injectable hydrogels for cartilage repair. We also include the novel hybrid injectable hydrogel incorporated with nanoparticles. Finally, we conclude with the advances of this clinical application and the challenges of injectable hydrogels used in cartilage.

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