scholarly journals Improved Bone Regeneration in Rabbit Bone Defects Using 3D Printed Composite Scaffolds Functionalized with Osteoinductive Factors

2020 ◽  
Vol 12 (43) ◽  
pp. 48340-48356
Author(s):  
Arun Kumar Teotia ◽  
Kasper Dienel ◽  
Irfan Qayoom ◽  
Bas van Bochove ◽  
Sneha Gupta ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
3D Printed ◽  
Rabbit Bone

scholarly journals Regeneration of Bone Defects in a Rabbit Femoral Osteonecrosis Model Using 3D-Printed Poly (Epsilon-Caprolactone)/Nanoparticulate Willemite Composite Scaffolds

2021 ◽  
Vol 22 (19) ◽  
pp. 10332
Author(s):  
Latifeh Karimzadeh Bardeei ◽  
Ehsan Seyedjafari ◽  
Ghamartaj Hossein ◽  
Mohammad Nabiuni ◽  
Mohammad Hosein Majles Ara ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Weight Bearing ◽  
Three Dimensional ◽  
Micro Computed Tomography ◽  
Composite Scaffolds ◽  
Functional Roles ◽  
Cellular Environment ◽  
3D Printed ◽  
Rabbit Bone ◽  
Regeneration Of Bone

Steroid-associated osteonecrosis (SAON) is a chronic disease that leads to the destruction and collapse of bone near the joint that is subjected to weight bearing, ultimately resulting in a loss of hip and knee function. Zn2+ ions, as an essential trace element, have functional roles in improving the immunophysiological cellular environment, accelerating bone regeneration, and inhibiting biofilm formation. In this study, we reconstruct SAON lesions with a three-dimensional (3D)-a printed composite made of poly (epsilon-caprolactone) (PCL) and nanoparticulate Willemite (npW). Rabbit bone marrow stem cells were used to evaluate the cytocompatibility and osteogenic differentiation capability of the PCL/npW composite scaffolds. The 2-month bone regeneration was assessed by a Micro-computed tomography (micro-CT) scan and the expression of bone regeneration proteins by Western blot. Compared with the neat PCL group, PCL/npW scaffolds exhibited significantly increased cytocompatibility and osteogenic activity. This finding reveals a new concept for the design of a 3D-printed PCL/npW composite-based bone substitute for the early treatment of osteonecrosis defects.

3D-printed magnetic Fe3O4/MBG/PCL composite scaffolds with multifunctionality of bone regeneration, local anticancer drug delivery and hyperthermia

2014 ◽  
Vol 2 (43) ◽  
pp. 7583-7595 ◽  
Author(s):  
Jianhua Zhang ◽  
Shichang Zhao ◽  
Min Zhu ◽  
Yufang Zhu ◽  
Yadong Zhang ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Bone Regeneration ◽  
Tumor Resection ◽  
Bone Defects ◽  
Composite Scaffolds ◽  
Anticancer Drug Delivery ◽  
3D Printed ◽  
Large Bone ◽  
Large Bone Defects ◽  
Magnetic Fe3o4

The 3D-printed Fe3O4/MBG/PCL scaffolds with potential multifunctionality would be promising for use in the treatment and regeneration of large bone defects after tumor resection.

3D-printed Poly-Lactic Co-Glycolic Acid (PLGA) scaffolds in non-critical bone defects impede bone regeneration in rabbit tibia bone

2021 ◽  
pp. 1-7
Author(s):  
Jin Xi Lim ◽  
Min He ◽  
Alphonsus Khin Sze Chong
Keyword(s):  
Computed Tomography ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Volume Ratio ◽  
Bone Defects ◽  
Control Group ◽  
Micro Computed Tomography ◽  
Rabbit Tibia ◽  
3D Printed ◽  
Critical Bone Defects

BACKGROUND: An increasing number of bone graft materials are commercially available and vary in their composition, mechanism of action, costs, and indications. OBJECTIVE: A commercially available PLGA scaffold produced using 3D printing technology has been used to promote the preservation of the alveolar socket after tooth extraction. We examined its influence on bone regeneration in long bones of New Zealand White rabbits. METHODS: 5.0-mm-diameter circular defects were created on the tibia bones of eight rabbits. Two groups were studied: (1) control group, in which the bone defects were left empty; (2) scaffold group, in which the PLGA scaffolds were implanted into the bone defect. Radiography was performed every two weeks postoperatively. After sacrifice, bone specimens were isolated and examined by micro-computed tomography and histology. RESULTS: Scaffolds were not degraded by eight weeks after surgery. Micro-computed tomography and histology showed that in the region of bone defects that was occupied by scaffolds, bone regeneration was compromised and the total bone volume/total volume ratio (BV/TV) was significantly lower. CONCLUSION: The implantation of this scaffold impedes bone regeneration in a non-critical bone defect. Implantation of bone scaffolds, if unnecessary, lead to a slower rate of fracture healing.

scholarly journals Fabrication and Characterization of Ta–GelMA–BG Scaffolds by Chemical Crosslinking Processing for Promotion Osteointegration

2021 ◽  
Vol 8 ◽  
Author(s):  
Fujian Zhao ◽  
Xiongfa Ji ◽  
Yang Yan ◽  
Zhen Yang ◽  
Xiaofeng Chen ◽  
...  
Keyword(s):  
Early Stage ◽  
Bone Defects ◽  
Chemical Crosslinking ◽  
Composite Scaffolds ◽  
Load Bearing ◽  
3D Printed ◽  
Fabrication And Characterization ◽  
Cell Adhesion And Proliferation

The repair of bone defects in load-bearing positions still faces great challenges. Tantalum (Ta) has attempted to repair bone defects based on the excellent mechanical properties. However, the osseointegration of Ta needs to be improved due to the lack of osteoinduction. Herein, tantalum–gelatin–methacryloyl–bioactive glass (Ta–GelMA–BG) scaffolds were successfully fabricated by loading BG in 3D-printed Ta scaffolds through a chemical crosslinking method. The results showed that the composite scaffolds have the ability to promote cell adhesion and proliferation. The incorporation of BG resulted in a significant increase in apatite-forming and osteogenesis differentiation abilities. In vivo results indicated that the Ta–GelMA–BG scaffolds significantly enhanced the osteointegration at the early stage after implantation. Overall, the Ta–GelMA–BG scaffolds are a promising platform for the load bearing bone regeneration field.

scholarly journals Repair of critical-size porcine craniofacial bone defects using a collagen-polycaprolactone composite biomaterial

2021 ◽  
Author(s):  
Marley J Dewey ◽  
Derek J Milner ◽  
Daniel Weisgerber ◽  
Colleen Flanagan ◽  
Marcello Rubessa ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Fibrous Tissue ◽  
Bone Defects ◽  
Large Animal ◽  
Collagen Scaffolds ◽  
Shape Fitting ◽  
Large Animal Models ◽  
3D Printed ◽  
Mineralized Collagen

Regenerative medicine approaches for massive craniomaxillofacial bone defects face challenges associated with the scale of missing bone, the need for rapid graft-defect integration, and challenges related to inflammation and infection. Mineralized collagen scaffolds have been shown to promote mesenchymal stem cell osteogenesis due to their porous nature and material properties, but are mechanically weak, limiting surgical practicality. Previously, these scaffolds were combined with 3D-printed polycaprolactone mesh to form a scaffold-mesh composite to increase strength and promote bone formation in sub-critical sized porcine ramus defects. Here, we compare the performance of mineralized collagen-polycaprolactone composites to the polycaprolactone mesh in a critical-sized porcine ramus defect model. While there were no differences in overall healing response between groups, our data demonstrated broadly variable metrics of healing regarding new bone infiltration and fibrous tissue formation. Abscesses were present surrounding some implants and polycaprolactone polymer was still present after 9-10 months of implantation. Overall, while there was limited successful healing, with 2 of 22 implants showed substantial levels of bone regeneration, and others demonstrating some form of new bone formation, the results suggest targeted improvements to improve repair of large animal models to more accurately represent craniomaxillofacial bone healing. Notably, strategies to increase osteogenesis throughout the implant, modulate the immune system to support repair, and employ shape-fitting tactics to avoid implant micromotion and resultant fibrosis. Improvements to the mineralized collagen scaffolds involve changes in pore size and shape to increase cell migration and osteogenesis and inclusion or delivery of factors to aid vascular ingrowth and bone regeneration.

scholarly journals Characterisation of bone regeneration in 3D printed ductile PCL/PEG/hydroxyapatite scaffolds with high ceramic microparticle concentrations

2021 ◽  
Author(s):  
Jing Yang ◽  
Chuanliang Cao ◽  
Pengren Huang ◽  
Aruna Prasopthum ◽  
Andy James Parsons ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bioactive Glass ◽  
Bone Tissue Engineering ◽  
Bone Regeneration ◽  
Particle Concentration ◽  
Composite Scaffolds ◽  
Reinforced Composite ◽  
3D Printed ◽  
Particle Reinforced Composite ◽  
Very High

3D printed bioactive glass or bioceramic particle reinforced composite scaffolds for bone tissue engineering currently suffer from low particle concentration (<50 wt%) hence low osteoconductivity. Meanwhile, composites with very high...

scholarly journals Biofabrication of 3D printed hydroxyapatite composite scaffolds for bone regeneration

2020 ◽  
Author(s):  
Yoontae Kim ◽  
Eun-Jin Lee ◽  
Albert V. Davydov ◽  
Stanislav Frukhbeyen ◽  
Jonathan E. Seppala ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffolds ◽  
Hydroxyapatite Composite ◽  
3D Printed

3D printed porous PLA/nHA composite scaffolds with enhanced osteogenesis and osteoconductivity in vivo for bone regeneration

2019 ◽  
Vol 14 (6) ◽  
pp. 065003 ◽  
Author(s):  
Xibao Chen ◽  
Chunxia Gao ◽  
Jiawei Jiang ◽  
Yaping Wu ◽  
Peizhi Zhu ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Scaffolds ◽  
3D Printed

scholarly journals Polydopamine functionalized VEGF gene-activated 3D printed scaffolds for bone regeneration

RSC Advances ◽  
2021 ◽  
Vol 11 (22) ◽  
pp. 13282-13291
Author(s):  
Jaidev L. Chakka ◽  
Timothy Acri ◽  
Noah Z. Laird ◽  
Ling Zhong ◽  
Kyungsup Shin ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Blood Vessels ◽  
Bone Defects ◽  
Vegf Gene ◽  
3D Printed ◽  
Critical Bone Defects

Bone is a highly vascularized organ and the formation of new blood vessels is essential to regenerate large critical bone defects.

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