scholarly journals Label-free colorimetric detection of glutathione by autocatalytic oxidation of o-phenylenediamine based on Au3+ regulation and its application

2021 ◽  
Author(s):  
Shaoguang Li ◽  
Huilin Liu ◽  
Xiaoying Zhang ◽  
Jiasong Cai ◽  
Kunlong Huang ◽  
...  

A label-free, rapid, and highly sensitive colorimetric assay for the detection of glutathione (GSH) was developed.

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.


Chemosensors ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 10 ◽  
Author(s):  
Wang ◽  
Guo ◽  
Hu ◽  
Liang ◽  
Li ◽  
...  

In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.


Talanta ◽  
2018 ◽  
Vol 186 ◽  
pp. 330-336 ◽  
Author(s):  
Fang Li ◽  
Jiachang Liu ◽  
Yuting Hu ◽  
Ning Deng ◽  
Jianbo He

2020 ◽  
Vol 2 (12) ◽  
pp. 5737-5745
Author(s):  
Tejaswini Appidi ◽  
Sushma V. Mudigunda ◽  
Suseela Kodandapani ◽  
Aravind Kumar Rengan

“C-ColAur” technique for colorimetric detection of cervical cancer by in situ formation of gold nanoparticles.


Toxins ◽  
2015 ◽  
Vol 7 (12) ◽  
pp. 5377-5385 ◽  
Author(s):  
Yunxia Luan ◽  
Jiayi Chen ◽  
Cheng Li ◽  
Gang Xie ◽  
Hailong Fu ◽  
...  

RSC Advances ◽  
2015 ◽  
Vol 5 (8) ◽  
pp. 6100-6105 ◽  
Author(s):  
Xue Gong ◽  
Wenjiao Zhou ◽  
Yaqin Chai ◽  
Yun Xiang ◽  
Ruo Yuan

Protection of ssDNA from digesting by Exo I generates amplified color transition for label-free and sensitive detection of folate receptors.


2015 ◽  
Vol 7 (13) ◽  
pp. 5600-5605 ◽  
Author(s):  
Bingfang Shi ◽  
Yingfeng Qin ◽  
Mengjiao Huang ◽  
Jingjin Zhao ◽  
Yubin Su ◽  
...  

A label-free colorimetric assay based on the catalysis of G-quadruplex/hemin DNAzyme was developed for highly sensitive and specific detection of S1 nuclease activity and inhibition.


2015 ◽  
Vol 51 (16) ◽  
pp. 3371-3374 ◽  
Author(s):  
Fengxia Su ◽  
Limei Wang ◽  
Yueying Sun ◽  
Chenghui Liu ◽  
Xinrui Duan ◽  
...  

Using LCR amplification and AuNP-based colorimetric detection, a highly sensitive and selective method for detection of DNA methylation has been developed.


2017 ◽  
Vol 53 (36) ◽  
pp. 5056-5058 ◽  
Author(s):  
Shouting Zhang ◽  
Zhuoyue Zhang ◽  
Tiansheng Wang ◽  
Dongxu Zhang ◽  
Xuemei Li ◽  
...  

Herein, we present a catalysis-based, label-free, and efficient strategy for a rapid, high-throughput, highly selective and ultrasensitive naked-eye colorimetric assay of Au3+ in aqueous solutions, based on the gold amalgam-stimulated peroxidase mimetic activity.


2013 ◽  
Vol 49 ◽  
pp. 20-24 ◽  
Author(s):  
Peng Miao ◽  
Tao Liu ◽  
Xiaoxi Li ◽  
Limin Ning ◽  
Jian Yin ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document