A G-quadruplex-based colorimetric assay of S1 nuclease activity and inhibition

2015 ◽  
Vol 7 (13) ◽  
pp. 5600-5605 ◽  
Author(s):  
Bingfang Shi ◽  
Yingfeng Qin ◽  
Mengjiao Huang ◽  
Jingjin Zhao ◽  
Yubin Su ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Nuclease Activity ◽  
Label Free ◽  
Specific Detection ◽  
S1 Nuclease ◽  
Highly Sensitive ◽  
G Quadruplex

A label-free colorimetric assay based on the catalysis of G-quadruplex/hemin DNAzyme was developed for highly sensitive and specific detection of S1 nuclease activity and inhibition.

scholarly journals A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity

The Analyst ◽  
2020 ◽  
Vol 145 (7) ◽  
pp. 2774-2778
Author(s):  
Jiahui Wen ◽  
Yongming Liu ◽  
Jingwen Li ◽  
Hao Lin ◽  
Yiran Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Method ◽  
Nuclease Activity ◽  
Colorimetric Sensor ◽  
Sensitive Detection ◽  
Label Free ◽  
S1 Nuclease ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

A label-free, simple and rapid colorimetric method for the sensitive detection of S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles.

A Highly Sensitive and Label-Free Microbead-Based ‘Turn-On’ Colorimetric Sensor for the Detection of Mercury(II) in Urine Using a Peroxidase-Like Split G-Quadruplex–Hemin DNAzyme

2018 ◽  
Vol 71 (12) ◽  
pp. 945
Author(s):  
Xin Fu ◽  
He Zhang ◽  
Jie Zhang ◽  
Shi-Tong Wen ◽  
Xing-Cheng Deng
Keyword(s):  
Standard Deviation ◽  
Base Pair ◽  
Limit Of Detection ◽  
Label Free ◽  
Turn On ◽  
Highly Sensitive ◽  
Relative Standard ◽  
G Quadruplex ◽  
Catalytically Active ◽  
Partial Cdna

A highly sensitive and label-free microbead-based ‘turn-on’ assay was developed for the detection of Hg2+ in urine based on the Hg2+-mediated formation of intermolecular split G-quadruplex–hemin DNAzymes. In the presence of Hg2+, T–T mismatches between the two partial cDNA strands were stabilized by a T–Hg2+–T base pair, and can cause the G-rich sequences of the two oligonucleotides to associate to form a split G-quadruplex which is able to bind hemin to form the catalytically active G-quadruplex–hemin DNAzyme. This microbead-based ‘turn-on’ process allows the detection of Hg2+ in urine samples at concentrations as low as 0.5 pM. The relative standard deviation and recovery are 1.2–3.9 and 98.7–103.2%, respectively. The remarkable sensitivity for Hg2+ is mainly attributed to the enhanced mass transport ability that is inherent in homogeneous microbead-based assays. Compared with previous developments of intermolecular split G-quardruplex–hemin DNAzymes for the homogeneous detection of Hg2+ (the limit of detection was 19nM), a signal enhancement of ~1000 times is obtained when such an assay is performed on the surface of microbeads.

Label-Free Electrical Immunosensor for Highly Sensitive and Specific Detection of Microcystin-LR in Water Samples

2015 ◽  
Vol 49 (15) ◽  
pp. 9256-9263 ◽  
Author(s):  
Feng Tan ◽  
Nuvia Maria Saucedo ◽  
Pankaj Ramnani ◽  
Ashok Mulchandani
Keyword(s):  
Water Samples ◽  
Label Free ◽  
Specific Detection ◽  
Highly Sensitive

Fluorescent S1 nuclease assay utilizing exponential strand displacement amplification

The Analyst ◽  
2019 ◽  
Vol 144 (10) ◽  
pp. 3364-3368 ◽  
Author(s):  
Chang Yeol Lee ◽  
Hansol Kim ◽  
Hyo Yong Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Nuclease Activity ◽  
Label Free ◽  
Strand Displacement ◽  
Activity Assay ◽  
Strand Displacement Amplification ◽  
S1 Nuclease ◽  
Nuclease Assay

We devise a simple, label-free S1 nuclease activity assay by exploiting target-induced inhibition of exponential strand displacement amplification (eSDA).

Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

G-quadruplex-Based Fluorescent Assay of S1 Nuclease Activity and K+

2013 ◽  
Vol 85 (4) ◽  
pp. 2431-2435 ◽  
Author(s):  
Zhixue Zhou ◽  
Jinbo Zhu ◽  
Libing Zhang ◽  
Yan Du ◽  
Shaojun Dong ◽  
...  
Keyword(s):  
Nuclease Activity ◽  
Fluorescent Assay ◽  
S1 Nuclease ◽  
G Quadruplex

A label-free G-quadruplex DNA-based fluorescence method for highly sensitive, direct detection of cisplatin

2014 ◽  
Vol 202 ◽  
pp. 714-720 ◽  
Author(s):  
Hualin Yang ◽  
Hanli Cui ◽  
Longgang Wang ◽  
Li Yan ◽  
Yong Qian ◽  
...  
Keyword(s):  
Direct Detection ◽  
Fluorescence Method ◽  
Label Free ◽  
Quadruplex Dna ◽  
Highly Sensitive ◽  
G Quadruplex

A highly sensitive and adjustable colorimetric assay of hydrogen sulfide by signal amplification based on G-quadruplex-Cu2+ peroxidase mimetics

The Analyst ◽  
2020 ◽  
Vol 145 (8) ◽  
pp. 2995-3001 ◽  
Author(s):  
Yu Ma ◽  
Maotian Xu ◽  
Yongxiang Wang ◽  
Zi Liu ◽  
Baoxian Ye
Keyword(s):  
Hydrogen Sulfide ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Highly Sensitive ◽  
G Quadruplex

This work reports the first example of a colorimetric H2S sensor constructed through G-quadruplex-Cu2+ (G4-Cu2+) peroxidase mimetics employing Cu2+ ions and G-rich DNA with signal amplification.

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