Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction

2015 ◽  
Vol 51 (16) ◽  
pp. 3371-3374 ◽  
Author(s):  
Fengxia Su ◽  
Limei Wang ◽  
Yueying Sun ◽  
Chenghui Liu ◽  
Xinrui Duan ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Genomic Dna ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Cpg Methylation ◽  
Chain Reaction ◽  
Selective Method ◽  
Ligase Chain Reaction ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Using LCR amplification and AuNP-based colorimetric detection, a highly sensitive and selective method for detection of DNA methylation has been developed.

Colorimetric detection of class A soybean saponins by coupling DNAzyme with the gap ligase chain reaction

2020 ◽  
Vol 12 (26) ◽  
pp. 3361-3367
Author(s):  
Wenshuai Li ◽  
Guorui Wu ◽  
Min Wang ◽  
Aiqin Yue ◽  
Weijun Du ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Naked Eye ◽  
Class A

We propose a colorimetric assay based on the coupling of gap ligase chain reaction (Gap-LCR) with DNAzyme to detect the target GmSg-1 genes of class A soybean saponins with the naked eye, without the involvement of expensive instruments.

Highly sensitive DNA methylation analysis at CpG resolution by surface-enhanced Raman scattering via ligase chain reaction

2015 ◽  
Vol 51 (54) ◽  
pp. 10953-10956 ◽  
Author(s):  
Yuling Wang ◽  
Eugene J. H. Wee ◽  
Matt Trau
Keyword(s):  
Dna Methylation ◽  
Raman Scattering ◽  
Methylation Analysis ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Highly Sensitive ◽  
Surface Enhanced ◽  
Surface Enhanced Raman ◽  
Enhanced Raman Scattering ◽  
Dna Methylation Analysis

Highly sensitive DNA methylation analysis at CpG resolution is demonstrated by employing SERS nanotags via ligase chain reaction (LCR) and validated with sequencing.

Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor

Nanoscale ◽  
2014 ◽  
Vol 6 (6) ◽  
pp. 3055-3058 ◽  
Author(s):  
Xinjian Yang ◽  
Zhiqiang Gao
Keyword(s):  
Gold Nanoparticles ◽  
Small Molecule ◽  
Signal Amplification ◽  
Colorimetric Assay ◽  
Folate Receptor ◽  
Colorimetric Detection ◽  
Label Free ◽  
Exonuclease Iii ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

By combining terminal protection of small molecule (folate)-capped DNA probes, exonuclease III signal amplification and gold nanoparticles, we developed a simple and label-free colorimetric assay for highly sensitive detection of folate receptor.

scholarly journals Highly sensitive and multiplexed analysis of CpG methylation at single-base resolution with ligation-based exponential amplification

2015 ◽  
Vol 6 (3) ◽  
pp. 1866-1872 ◽  
Author(s):  
Fengxia Su ◽  
Limei Wang ◽  
Yueying Sun ◽  
Chenghui Liu ◽  
Xinrui Duan ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Sensitivity ◽  
Cpg Methylation ◽  
Chain Reaction ◽  
Single Base ◽  
Ligase Chain Reaction ◽  
Highly Sensitive ◽  
Single Base Resolution ◽  
Multiplexed Analysis

Multiple CpG methylation can be accurately detected in one-tube ligase chain reaction (LCR) amplification with high sensitivity and specificity.

Lab in a tube: a fast-assembled colorimetric sensor for highly sensitive detection of oligonucleotides based on a hybridization chain reaction

RSC Advances ◽  
2015 ◽  
Vol 5 (55) ◽  
pp. 44714-44721 ◽  
Author(s):  
Siqi Zhang ◽  
Kun Wang ◽  
Zhenyu Li ◽  
Zhongmin Feng ◽  
Ting Sun
Keyword(s):  
Complex Formation ◽  
Dna Sequence ◽  
Self Assembly ◽  
The Self ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
G Quadruplex ◽  
Colored Product ◽  
Highly Sensitive Detection

Upon adding THBV, the self-assembly of THBV with H1 allows the rest of the DNA sequence of H1 to accelerate H1–H2 complex formation. The G-quadruplex at the end of the H1–H2 complex could catalyze TMB into a colored product.

Zwitterionic peptide-capped gold nanoparticles for colorimetric detection of Ni2+

Nanoscale ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 5466-5473 ◽  
Author(s):  
Attasith Parnsubsakul ◽  
Sukunya Oaew ◽  
Werasak Surareungchai
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Sensitive Detection ◽  
Highly Sensitive ◽  
Highly Sensitive Detection

Recyclable zwitterionic peptide-capped AuNPs for highly sensitive detection of Ni2+.

Direct fluorescence detection of point mutations in human genomic DNA using microbead-based ligase chain reaction

Talanta ◽  
2010 ◽  
Vol 80 (5) ◽  
pp. 1725-1729 ◽  
Author(s):  
Xiangxian Meng ◽  
Xiaohai Yang ◽  
Kemin Wang ◽  
Qiuping Guo ◽  
Yongjun Tan ◽  
...  
Keyword(s):  
Fluorescence Detection ◽  
Genomic Dna ◽  
Point Mutations ◽  
Chain Reaction ◽  
Ligase Chain Reaction ◽  
Direct Fluorescence ◽  
Human Genomic ◽  
Human Genomic Dna

Evaluation of a 7-Day Continuous Intravenous Infusion of Decitabine: Inhibition of Promoter-Specific and Global Genomic DNA Methylation

2005 ◽  
Vol 23 (17) ◽  
pp. 3897-3905 ◽  
Author(s):  
Wolfram E. Samlowski ◽  
Sancy A. Leachman ◽  
Mark Wade ◽  
Pamela Cassidy ◽  
Patricia Porter-Gill ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Polymerase Chain Reaction ◽  
Continuous Infusion ◽  
Intravenous Infusion ◽  
Genomic Dna ◽  
Quantitative Polymerase Chain Reaction ◽  
Dna Hypomethylation ◽  
Chain Reaction ◽  
Polymerase Chain

Purpose The nucleoside analog 5-aza-2′-deoxycytidine (5-aza-CdR, decitabine) is a potent inhibitor of DNA methylation in vitro. Cellular treatment with this agent induces the re-expression of methylation-silenced genes. It remains unclear to what extent this compound inhibits DNA methylation in vivo. A clinical study was designed to examine the molecular effects and toxicity of a continuous 1-week intravenous infusion of decitabine in solid tumor patients. Methods Ten patients with refractory solid tumors were included in this study. Decitabine was administered at 2 mg/m2/d via continuous infusion for 168 hours. Quantitative polymerase chain reaction and high performance liquid chromatography were utilized to measure promoter-specific and global DNA methylation in peripheral-blood cells before and after treatment. Results Transient grade III/IV neutropenia (two patients) and grade II thrombocytopenia (one patient) was observed at the lowest planned dose step (2 mg/m2/d for 7 days). Nonhematologic toxicities were not observed. Quantitative polymerase chain reaction demonstrated significant MAGE-1 promoter hypomethylation by 14 days after the start of treatment in all 13 treatment cycles examined. Significant genomic DNA hypomethylation was also seen by day 14 in 11 of 13 treatment cycles analyzed. Genomic DNA methylation reverted to baseline levels by 28 to 35 days after the start of treatment, demonstrating that inhibition of DNA methylation by decitabine is transient. Conclusion A 168-hour continuous infusion of decitabine is well tolerated and results in the inhibition of promoter-specific and genomic DNA methylation in vivo. This treatment schedule is suitable for evaluation of decitabine in combination with agents whose activity may be enhanced by the reversal of DNA methylation–mediated gene silencing.

scholarly journals Highly sensitive detection of lipopolysaccharides using an aptasensor based on hybridization chain reaction

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Peiyan Xie ◽  
Longjiao Zhu ◽  
Xiangli Shao ◽  
Kunlun Huang ◽  
Jingjing Tian ◽  
...  
Keyword(s):  
Sensitive Detection ◽  
Hybridization Chain Reaction ◽  
Chain Reaction ◽  
Highly Sensitive ◽  
Highly Sensitive Detection
Sign in / Sign up

Export Citation Format

Share Document