Three-dimensionally printable shear-thinning triblock copolypeptide hydrogels with antimicrobial potency

2021 ◽  
Author(s):  
Robert Murphy ◽  
Shadi Kordbacheh ◽  
Dimitrios Skoulas ◽  
Simon Ng ◽  
Kasinan Suthiwanich ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Rational Design ◽  
Shear Thinning ◽  
Block Sequence ◽  
Central Block ◽  
Antimicrobial Potency

Through rational design, block sequence controlled triblock copolypeptides comprising cysteine and tyrosine as well as a lysine or glutamic acid central block are devised. In these copolypeptides, each block contributes...

scholarly journals Rational Design of Network Properties in Guest–Host Assembled and Shear-Thinning Hyaluronic Acid Hydrogels

2013 ◽  
Vol 14 (11) ◽  
pp. 4125-4134 ◽  
Author(s):  
Christopher B. Rodell ◽  
Adam L. Kaminski ◽  
Jason A. Burdick
Keyword(s):  
Hyaluronic Acid ◽  
Rational Design ◽  
Shear Thinning ◽  
Network Properties

Rational design and synthesis of potent and long-lasting glutamic acid-based dipeptidyl peptidase IV inhibitors

2009 ◽  
Vol 19 (7) ◽  
pp. 1908-1912 ◽  
Author(s):  
Ting-Yueh Tsai ◽  
Tsu Hsu ◽  
Chiung-Tong Chen ◽  
Jai-Hong Cheng ◽  
Mei-Chun Chiou ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
Rational Design ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Design And Synthesis ◽  
Dipeptidyl Peptidase Iv Inhibitors

Glutamic acid and its derivatives: candidates for rational design of anticancer drugs

2013 ◽  
Vol 5 (8) ◽  
pp. 961-978 ◽  
Author(s):  
Imran Ali ◽  
Waseem A Wani ◽  
Ashanul Haque ◽  
Kishwar Saleem
Keyword(s):  
Glutamic Acid ◽  
Anticancer Drugs ◽  
Rational Design

Rational design of shear-thinning supramolecular hydrogels with porphyrin for controlled chemotherapeutics release and photodynamic therapy

2015 ◽  
Vol 66 ◽  
pp. 149-159 ◽  
Author(s):  
Hua Jin ◽  
Xiao-Hui Dai ◽  
Chuan Wu ◽  
Jian-Ming Pan ◽  
Xiao-Hong Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Rational Design ◽  
Shear Thinning

scholarly journals Rational design of novel, fluorescent, tagged glutamic acid dendrimers with different terminal groups and in silico analysis of their properties

2017 ◽  
Vol Volume 12 ◽  
pp. 7053-7073 ◽  
Author(s):  
Nuno Martinho ◽  
Liana Silva ◽  
Helena Florindo ◽  
Steve Brocchini ◽  
Mire Zloh ◽  
...  
Keyword(s):  
Glutamic Acid ◽  
In Silico ◽  
Rational Design ◽  
In Silico Analysis ◽  
Silico Analysis

Self-Assembled Nanostructure Library from Monodisperse Sequence-Defined Oligo(phosphodiester)s

2019 ◽  
Author(s):  
Donatien de Rochambeau ◽  
Maciej Barlog ◽  
Hassan S. Bazzi ◽  
Hanadi Sleiman
Keyword(s):  
Chemical Composition ◽  
Self Assembly ◽  
Rational Design ◽  
Morphological Changes ◽  
Monomer Unit ◽  
Information Storage ◽  
Supramolecular Interactions ◽  
Block Sequence ◽  
Micelle Size ◽  
Fast Synthesis

<p><a>Natural biopolymers achieve information storage, molecular recognition and catalysis efficiently through sequence-control. To be able to mimic such properties, self-assembly studies of artificial sequence-defined oligomers is of great interest. In this paper, we show the use of hydrophilic, lipophilic, aromatic and fluorophilic monomers to synthesize a large library of truly monodisperse sequence-defined block co-oligo(phosphodiester)s. Automated and accurate control over the sequence allowed to rationally study the degree of polymerisation, blocks ratio, chemical composition and orthogonal supramolecular interactions influence on self-assembly. Interestingly, our studies revealed remarkable morphological changes (spheres to nanosheets) caused by very small differences between polymers, e.g., polymers differing by a single monomer unit. Inverting block sequence in multi-block copolymers also caused a dramatic increase in micelle size. Conventional polymerization does not allow the exploration of these subtle variations in polymer sequence or composition. Therefore, fast synthesis and purification of a variety of oligomers with slightly different sequences allows studying the supramolecular chemistry of precision oligomers in a systematic way. It paves the way to the rational design of functional sequence-defined polymers.</a></p>

scholarly journals Computational and experimental investigation of biofilm disruption dynamics induced by high velocity gas jet impingement

2019 ◽  
Author(s):  
Lledó Prades ◽  
Stefania Fabbri ◽  
Antonio D. Dorado ◽  
Xavier Gamisans ◽  
Paul Stoodley ◽  
...  
Keyword(s):  
High Speed ◽  
Rational Design ◽  
Shear Thinning ◽  
High Shear ◽  
Shear Rates ◽  
Air Jet ◽  
High Shear Rates ◽  
Applied Forces ◽  
Biofilm Disruption ◽  
The Impact

ABSTRACTExperimental data showed that high-speed micro-sprays can effectively disrupt biofilms on their support substratum, producing a variety of dynamic reactions such as elongation, displacement, ripples formation and fluidization. However, the mechanics underlying the impact of high-speed turbulent flows on biofilm structure is complex in such extreme conditions, since direct measurements of viscosity at these high shear rates are not possible using dynamic testing instruments. Here we used computational fluid dynamics simulations to assess the complex fluid interactions of ripple patterning produced by high-speed turbulent air jets impacting perpendicular to the surface of Streptococcus mutans biofilms, a dental pathogen causing caries, captured by high speed imaging. The numerical model involved a two-phase flow of air over a non-Newtonian biofilm, whose viscosity as a function of shear rate was estimated using the Herschel-Bulkley model. The simulation suggested that inertial, shear and interfacial tension forces governed biofilm disruption by the air jet. Additionally, the high shear rates generated by the jet impacts coupled with shear-thinning biofilm property resulted in rapid liquefaction (within milliseconds) of the biofilm, followed by surface instability and travelling waves from the impact site. Our findings suggest that rapid shear-thinning in the biofilm reproduces dynamics under very high shear flows that elasticity can be neglected under these conditions, behaving the biofilm as a Newtonian fluid. A parametric sensitivity study confirmed that both applied force intensity (i.e. high jet-nozzle air velocity) and biofilm properties (i.e. low viscosity, low air-biofilm surface tension and thickness) intensify biofilm disruption, by generating large interfacial instabilities.IMPORTANCEKnowledge of mechanisms promoting disruption though mechanical forces is essential in optimizing biofilm control strategies which rely on fluid shear. Our results provide insight into how biofilm disruption dynamics is governed by applied forces and fluid properties, revealing a mechanism for ripples formation and fluid-biofilm mixing. These findings have important implications for the rational design of new biofilms cleaning strategies with fluid jets, such as determining optimal parameters (e.g. jet velocity and position) to remove the biofilm from a certain zone (e.g. in dental hygiene or debridement of surgical site infections), or using antimicrobial agents which could increase the interfacial area available for exchange, as well as causing internal mixing within the biofilm matrix, thus disrupting the localized microenvironment which is associated with antimicrobial tolerance. The developed model also has potential application in predicting drag and pressure drop caused by biofilms on bioreactor, pipeline and ship hull surfaces.

Self-Assembled Nanostructure Library from Monodisperse Sequence-Defined Oligo(phosphodiester)s

2019 ◽  
Author(s):  
Donatien de Rochambeau ◽  
Maciej Barlog ◽  
Hassan S. Bazzi ◽  
Hanadi Sleiman
Keyword(s):  
Chemical Composition ◽  
Self Assembly ◽  
Rational Design ◽  
Morphological Changes ◽  
Monomer Unit ◽  
Information Storage ◽  
Supramolecular Interactions ◽  
Block Sequence ◽  
Micelle Size ◽  
Fast Synthesis

<p><a>Natural biopolymers achieve information storage, molecular recognition and catalysis efficiently through sequence-control. To be able to mimic such properties, self-assembly studies of artificial sequence-defined oligomers is of great interest. In this paper, we show the use of hydrophilic, lipophilic, aromatic and fluorophilic monomers to synthesize a large library of truly monodisperse sequence-defined block co-oligo(phosphodiester)s. Automated and accurate control over the sequence allowed to rationally study the degree of polymerisation, blocks ratio, chemical composition and orthogonal supramolecular interactions influence on self-assembly. Interestingly, our studies revealed remarkable morphological changes (spheres to nanosheets) caused by very small differences between polymers, e.g., polymers differing by a single monomer unit. Inverting block sequence in multi-block copolymers also caused a dramatic increase in micelle size. Conventional polymerization does not allow the exploration of these subtle variations in polymer sequence or composition. Therefore, fast synthesis and purification of a variety of oligomers with slightly different sequences allows studying the supramolecular chemistry of precision oligomers in a systematic way. It paves the way to the rational design of functional sequence-defined polymers.</a></p>

RATIONAL DESIGN, SYNTHESIS AND IN VITRO EVALUATION OF N-(4-ETHOXYPHENYLSULFONYL)-L-GLUTAMIC ACID ANALOGUES AS ANTIANGIOGENIC AND ANTICANCER AGENTS ON MULTIPLE MYELOMA

INDIAN DRUGS ◽  
2021 ◽  
Vol 57 (12) ◽  
pp. 26-35
Author(s):  
Abhijit Saha ◽  
Koushik Sarker ◽  
Avijit Ghosh ◽  
Suvasish Mishra ◽  
Subrata Sen ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
Glutamic Acid ◽  
Anticancer Agents ◽  
Rational Design ◽  
Umbilical Vein ◽  
Cytotoxic Action ◽  
Molecular Docking Study ◽  
Design Synthesis ◽  
African Green Monkey Kidney

We report the rational design, synthesis and evaluation of the anticancer and antiangiogenic activity of the N-(4-ethoxyphenylsulfonyl)-L-glutamic acid analogs on multiple myeloma. From the series, compound 2c, 2f, and 2h exhibit cytotoxic action on human multiple myeloma cell line RPMI8226 with IC50 (μM) value 2.72, 2.24, and 1.81, respectively. These compounds possess the antiangiogenic property and are selectively cytotoxic to cancer cells, as observed from the in vitro study of Human Umbilical Vein Endothelial Cell (HUVEC) and African green monkey kidney epithelial cell (VERO), respectively. The compounds also have an antiproliferative effect on HUVECs, which was carried out using the dye exclusion method with trypan blue. Vascular Endothelial Growth Factor Receptor-2 (VEGFR-2) Tyr-1175 phosphorylation inhibition assay showed compound 2f, and 2h to be the active inhibitors of roangiogenic responses mediated by VEGFR-2. A molecular docking study of 2f with VEGFR-2 showed possible interaction with a binding energy of -74.19 kcal/mol.

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