scholarly journals Rational Design of Network Properties in Guest–Host Assembled and Shear-Thinning Hyaluronic Acid Hydrogels

2013 ◽  
Vol 14 (11) ◽  
pp. 4125-4134 ◽  
Author(s):  
Christopher B. Rodell ◽  
Adam L. Kaminski ◽  
Jason A. Burdick
Keyword(s):  
Hyaluronic Acid ◽  
Rational Design ◽  
Shear Thinning ◽  
Network Properties

Epithelial cell adhesion on films mimicking surface of polymeric scaffolds of artificial urethra compared to molecular modeling of integrin binding

2019 ◽  
Vol 34 (3) ◽  
pp. 280-290 ◽  
Author(s):  
Jakub Kollar ◽  
Andrea Morelli ◽  
Federica Chiellini ◽  
Stanislav Miertus ◽  
Dusan Bakos ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Cell Adhesion ◽  
Epithelial Cell ◽  
Polymer Films ◽  
Rational Design ◽  
Computational Study ◽  
Transmembrane Protein ◽  
Molecular Mechanic ◽  
Three Dimensional ◽  
Film Surface

In this study, a combined experimental and computational study of long-term human bladder epithelial cell line HBLAK adhesion and proliferation on five different polymeric surfaces, namely hyaluronic acid, amylose, collagen, polyhydroxybutyrate, and polylactic acid, was performed with the goal to understand the nature of the attraction between various surface materials and a simplified model of the cell surface (transmembrane protein integrin). These biodegradable polymers are frequently used as scaffolds for tissue engineering. During formation of the new tissue, the scaffold polymers are gradually replaced by the natural extracellular matrix of the proliferating cells. Cell adhesion and proliferation experiments were carried out employing thin polymer films prepared by solvent casting while for the computational approach three-dimensional molecular models of layers of ordered polymeric fibers were used as quasi-planar nano-sized models of polymeric surface patches. Experimental results indicated a good capability of amylose, polyhydroxybutyrate, and hyaluronic acid polymer films to foster cell adhesion. Proliferation experiment, carried out by incubating cells with the investigated polymer films for 72 h, showed that all the investigated polymers are able to sustain a good proliferation of HBLAK cells almost comparable to plain glass. Computational estimate of molecular mechanic interaction energies between three-dimensional models of polymeric films and the collagen-binding α2 I domain of the cell adhesion receptor integrin α2β1 confirmed elevated affinity to amylose and polyhydroxybutyrate that is related to higher polarity of function groups on the film surface as documented by the maps of molecular electrostatic potential. This combined experimental and modeling approach can contribute to rational design and surface modifications of polymeric material suitable for forming the scaffolds of human urethra that can be effectively colonized by stem cells.

scholarly journals Characterisation of Hyaluronic Acid Blends Modified by Poly(N-Vinylpyrrolidone)

Molecules ◽  
2021 ◽  
Vol 26 (17) ◽  
pp. 5233
Author(s):  
Katarzyna Lewandowska ◽  
Marta Szulc
Keyword(s):  
Hyaluronic Acid ◽  
Shear Thinning ◽  
Tensile Tests ◽  
Infrared Analysis ◽  
Shear Tests ◽  
Casting Method ◽  
Pronounced Increase ◽  
Blend Films ◽  
Solution Casting Method ◽  
Thinning Effect

The viscosity behaviour and physical properties of blends containing hyaluronic acid (HA) and poly(N-vinylpyrrolidone) (PVP) were studied by the viscometric technique, steady shear tests, tensile tests and infrared spectroscopy. Viscometric and rheological measurements were carried out using blends of HA/PVP with different HA weight fractions (0, 0.2, 0.5, 0.8 and 1). The polymer films and HA/PVP blend films were prepared using the solution casting method. The study of HA blends by viscometry showed that HA/PVP was miscible with the exception of the blend with high HA content. HA and its blends showed a shear-thinning flow behaviour. The non-Newtonian indices (n) of HA/PVP blends were calculated by the Ostwald–de Waele equation, indicating a shear-thinning effect in which pseudoplasticity increased with increasing HA contents. Mechanical properties, such as tensile strength and elongation at the break, were higher for HA/PVP films with wHA = 0.5 compared to those with higher HA contents. The elongation at the break of HA/PVP blend films displayed a pronounced increase compared to HA films. Moreover, infrared analysis confirmed the existence of interactions between HA and PVP. The blending of HA with PVP generated films with elasticity and better properties than homopolymer films.

scholarly journals Rational Design of Hyaluronic Acid-Based Copolymer-Mixed Micelle in Combination PD-L1 Immune Checkpoint Blockade for Enhanced Chemo-Immunotherapy of Melanoma

2021 ◽  
Vol 9 ◽  
Author(s):  
Chaopei Zhou ◽  
Xiuxiu Dong ◽  
Chunxiang Song ◽  
Shuang Cui ◽  
Tiantian Chen ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Tumor Cells ◽  
Immune Checkpoint ◽  
Targeted Delivery ◽  
Rational Design ◽  
Mixed Micelle ◽  
Tumor Therapy ◽  
Immune Checkpoint Blockade ◽  
Checkpoint Blockade ◽  
Therapy Strategy

The application of combinational therapy breaks the limitation of monotherapy and achieves better clinical benefit for tumor therapy. Herein, a hyaluronic acid/Pluronic F68-based copolymer-mixed micelle was constructed for targeted delivery of chemotherapeutical agent docetaxel (PHDM) in combination with programmed cell death ligand-1(PD-L1) antibody. When PHDM+anti-PDL1 was injected into the blood system, PHDM could accumulate into tumor sites and target tumor cells via CD44-mediated endocytosis and possess tumor chemotherapy. While anti-PDL1 could target PD-L1 protein expressed on surface of tumor cells to the immune checkpoint blockade characteristic for tumor immunotherapy. This strategy could not only directly kill tumor cells but also improve CD8+ T cell level and facilitate effector cytokines release. In conclusion, the rational-designed PHDM+anti-PDL1 therapy strategy creates a new way for tumor immune-chemotherapy.

Rational design of porous starch/hyaluronic acid composites for hemostasis

2020 ◽  
Vol 158 ◽  
pp. 1319-1329 ◽  
Author(s):  
Yanling Wang ◽  
Guangwan Liu ◽  
Lijuan Wu ◽  
Hongyuan Qu ◽  
Deli Song ◽  
...  
Keyword(s):  
Hyaluronic Acid ◽  
Rational Design

Rational design of shear-thinning supramolecular hydrogels with porphyrin for controlled chemotherapeutics release and photodynamic therapy

2015 ◽  
Vol 66 ◽  
pp. 149-159 ◽  
Author(s):  
Hua Jin ◽  
Xiao-Hui Dai ◽  
Chuan Wu ◽  
Jian-Ming Pan ◽  
Xiao-Hong Wang ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Rational Design ◽  
Shear Thinning

scholarly journals Intermediate structural hierarchy in biological networks modulates the fractal dimension and force distribution of percolating clusters

2021 ◽  
Author(s):  
Benjamin S Hanson ◽  
Lorna Dougan
Keyword(s):  
Biological Networks ◽  
Building Block ◽  
Rational Design ◽  
Network Formation ◽  
Dynamic Network ◽  
Force Transmission ◽  
Structural Hierarchy ◽  
Network Properties ◽  
Protein Hydrogels ◽  
Dynamic Network Formation

Globular protein hydrogels are an emerging class of materials with the potential for rational design, and a generalised understanding of how their network properties emerge from the structure and dynamics of the building block is a key challenge. Here we computationally investigate the effect of intermediate (polymeric) nanoscale structure on the formation of protein hydrogels. We show that changes in both the cross-link topology and flexibility of the polymeric building block lead to changes in the force transmission around the system, and provide insight into the dynamic network formation processes.

scholarly journals Shear-thinning hyaluronic acid hydrogels for insulin delivery

2016 ◽  
Vol 4 ◽  
Author(s):  
Xue Kun ◽  
Webber Matthew ◽  
Langer Robert ◽  
Anderson Daniel
Keyword(s):  
Hyaluronic Acid ◽  
Insulin Delivery ◽  
Shear Thinning
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