VEGFR2-targeted ultrasound molecular imaging of angiogenesis to evaluate liver allograft fibrosis

2021 ◽  
Author(s):  
Chen Qiu ◽  
Tingting Sha ◽  
Tinghui Yin ◽  
Wei Zhang ◽  
Xiuling Chen ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Monitoring Tool ◽  
Liver Allograft ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

The study highlighted the potential of VEGFR2-targeted USMI as an effective monitoring tool for liver allograft fibrosis.

VEGFR2-Targeted Ultrasound Molecular Imaging of Angiogenesis to Evaluate Liver Allograft Fibrosis

2021 ◽  
Author(s):  
Chen Qiu ◽  
Tingting Sha ◽  
Tinghui Yin ◽  
Wei Zhang ◽  
Xiuling Chen ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Liver Allograft ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

scholarly journals Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

2015 ◽  
Vol 31 (2) ◽  
pp. 90-106 ◽  
Author(s):  
Tom van Rooij ◽  
Verya Daeichin ◽  
Ilya Skachkov ◽  
Nico de Jong ◽  
Klazina Kooiman
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

Abstract 2482: Fate of Targeted Ultrasound Contrast Agents After Endothelial Adhesion: A Time Course Study

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Shivani Bowry ◽  
Jianjun Wang ◽  
Sue Ottoboni ◽  
Tom Ottoboni ◽  
William R Wagner ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Ultrasound Imaging ◽  
Time Course ◽  
Time Window ◽  
Leukocyte Adhesion ◽  
Ultrasound Contrast Agents ◽  
Acoustic Properties ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging ◽  
Time Course Study

Background: Targeted microsphere (μSPH) adhesion to endothelial epitopes forms the basis for ultrasound molecular imaging. The fate of targeted μSPH, once bound to the endothelial target, is unknown. This has limited the optimization of ultrasound imaging strategies; for example, the appropriate time to commence imaging after μSPH injection is uncertain, and in part depends on the time course and duration of μSPH binding. We therefore sought to characterize the behavior of adhered μSPH as a function of time using serial observations of rat cremaster microcirculation after μSPH injection. Methods: Fluorescent nitrogen-encapsulated polymer μSPH (diameter 3.0 ± 1.4 μm) bearing control non-specific IgG (μSPH CTL ) or monoclonal antibody against VCAM (μSPH VCAM ) or ICAM (μSPH ICAM ) were prepared. Inflamed cremaster muscle of 21 anesthetized Wistar rats was exteriorized for intravital microscopy 4 hours after intrascrotal injection of TNF-α (5μg). Each rat received 1 venous injection of a single μSPH species (n=7 rats/μSPH; 10 8 μSPH/injection). An index venule was identified for serial microscopic observation every 5 min starting 10 min after μSPH injection. Results: The cumulative sum of adhered μSPH during 1 hr observation was higher for μSPH ICAM (24±11) and μSPH VCAM (18±1) than μSPH CTL (3±2, p=0.005). The number of μSPH ICAM adherent to the index venule was stable over time (10 min: 2 ± 3; 30 min: 3±2; 80 min: 3 ± 5, ANOVA p=0.9). The number of adhered μSPH VCAM trended downwards (10 min: 5±4; 30 min: 2±2; 80 min: 1±2; ANOVA p=0.08). By 1 hr, adhered μSPH appeared altered in shape; no μSPH transmigration was seen. Conclusions: Nitrogen-filled polymer μSPH targeted to leukocyte adhesion molecules adhere to inflamed endothelium. The extent of μSPH adhesion remains relatively constant over a time period relevant for imaging applications, although beyond 1hr, the μSPH appear morphologically altered and could have different acoustic properties. These data provide a useful and broad time window for effective ultrasound imaging of molecular targets. The time course of μSPH attachment to endothelium is an important consideration when devising strategies for ultrasound molecular imaging and should be characterized in a μSPH- and target- specific manner.

Aptamer-Conjugated Nanobubbles for Targeted Ultrasound Molecular Imaging

Langmuir ◽  
2011 ◽  
Vol 27 (11) ◽  
pp. 6971-6976 ◽  
Author(s):  
Chung-Hsin Wang ◽  
Yu-Fen Huang ◽  
Chih-Kuang Yeh
Keyword(s):  
Molecular Imaging ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

Low intensity focused ultrasound (LIFU) triggered drug release from cetuximab-conjugated phase-changeable nanoparticles for precision theranostics against anaplastic thyroid carcinoma

2019 ◽  
Vol 7 (1) ◽  
pp. 196-210 ◽  
Author(s):  
Yang Wang ◽  
Guoqing Sui ◽  
Dengke Teng ◽  
Qimeihui Wang ◽  
Jia Qu ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Drug Release ◽  
Thyroid Carcinoma ◽  
Focused Ultrasound ◽  
Anaplastic Thyroid Carcinoma ◽  
Antitumor Therapy ◽  
Low Intensity ◽  
Targeted Ultrasound ◽  
Triggered Drug Release ◽  
Ultrasound Molecular Imaging

This study provides an efficient theranostic strategy for concurrent targeted ultrasound molecular imaging and effective synergistic antitumor therapy.

scholarly journals Intracolic ultrasound molecular imaging: a novel method for assessing colonic tumor necrosis factor-α expression in inflammatory bowel disease

2021 ◽  
Vol 27 (1) ◽  
Author(s):  
Xiaoyan Miao ◽  
Ren Mao ◽  
Yujia You ◽  
Huichao Zhou ◽  
Chen Qiu ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Ultrasound Imaging ◽  
Intestinal Mucosa ◽  
Tumor Necrosis ◽  
Regression Analyses ◽  
Targeted Ultrasound ◽  
Tnf Α ◽  
Inflammatory Bowel ◽  
Ultrasound Molecular Imaging ◽  
Necrosis Factor

Abstract Background While anti-tumor necrosis factor alpha (TNF-α) therapy has been proven effective in inflammatory bowel disease (IBD), approximately 40% of patients lose the response. Transmembrane TNF-α (mTNF-α) expression in the intestinal mucosa is correlated with therapeutic efficacy, and quantification of mTNF-α expression is significant for predicting response. However, conventional intravenous application of microbubbles is unable to assess mTNF-α expression in intestinal mucosa. Herein, we proposed intracolic ultrasound molecular imaging with TNF-α-targeted microbubbles (MBTNF-α) to quantitatively detect mTNF-α expression in the intestinal mucosa. Methods MBTNF-α was synthesized via a biotin–streptavidin bridging method. TNF-α-targeted ultrasound imaging was performed by intracolic application of MBTNF-α to detect mTNF-α expression in surgical specimens from a murine model and patients with IBD. Linear regression analyses were performed to confirm the accuracy of quantitative targeted ultrasound imaging. Results On quantitative TNF-α-targeted ultrasound images, a greater signal intensity was observed in the mouse colons with colitis ([1.96 ± 0.45] × 106 a.u.) compared to that of the controls ([0.56 ± 0.21] × 106 a.u., P < 0.001). Targeted US signal intensities and inflammatory lesions were topographically coupled in mouse colons. Linear regression analyses in specimens of mice and patients demonstrated significant correlations between the targeted ultrasound signal intensity and mTNF-α expression (both P < 0.001). Furthermore, TNF-α-targeted ultrasound imaging qualitatively distinguished the varying inflammatory severity in intestinal specimens from IBD patients. Conclusion Intracolic ultrasound molecular imaging with MBTNF-α enables quantitative assessment of mTNF-α expression. It may be a potential tool for facilitating the implementation of personalized medicine in IBD.

scholarly journals Breast Cancer Detection by B7-H3–Targeted Ultrasound Molecular Imaging

2015 ◽  
Vol 75 (12) ◽  
pp. 2501-2509 ◽  
Author(s):  
Sunitha V. Bachawal ◽  
Kristin C. Jensen ◽  
Katheryne E. Wilson ◽  
Lu Tian ◽  
Amelie M. Lutz ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Imaging ◽  
Cancer Detection ◽  
Breast Cancer Detection ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging
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