Low intensity focused ultrasound (LIFU) triggered drug release from cetuximab-conjugated phase-changeable nanoparticles for precision theranostics against anaplastic thyroid carcinoma

2019 ◽  
Vol 7 (1) ◽  
pp. 196-210 ◽  
Author(s):  
Yang Wang ◽  
Guoqing Sui ◽  
Dengke Teng ◽  
Qimeihui Wang ◽  
Jia Qu ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Drug Release ◽  
Thyroid Carcinoma ◽  
Focused Ultrasound ◽  
Anaplastic Thyroid Carcinoma ◽  
Antitumor Therapy ◽  
Low Intensity ◽  
Targeted Ultrasound ◽  
Triggered Drug Release ◽  
Ultrasound Molecular Imaging

This study provides an efficient theranostic strategy for concurrent targeted ultrasound molecular imaging and effective synergistic antitumor therapy.

VEGFR2-Targeted Ultrasound Molecular Imaging of Angiogenesis to Evaluate Liver Allograft Fibrosis

2021 ◽  
Author(s):  
Chen Qiu ◽  
Tingting Sha ◽  
Tinghui Yin ◽  
Wei Zhang ◽  
Xiuling Chen ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Liver Allograft ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

scholarly journals Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

2015 ◽  
Vol 31 (2) ◽  
pp. 90-106 ◽  
Author(s):  
Tom van Rooij ◽  
Verya Daeichin ◽  
Ilya Skachkov ◽  
Nico de Jong ◽  
Klazina Kooiman
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging

scholarly journals Cetuximab-Conjugated Perfluorohexane/Gold Nanoparticles for Low Intensity Focused Ultrasound Diagnosis Ablation of Thyroid Cancer Treatment

2020 ◽  
Author(s):  
Yue Ma ◽  
Lingling Wang ◽  
Haixia Li ◽  
Wen Cheng ◽  
Xiulan Zheng ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Thyroid Cancer ◽  
Thyroid Carcinoma ◽  
Cancer Treatment ◽  
Focused Ultrasound ◽  
Ultrasound Diagnosis ◽  
Human Thyroid ◽  
Rat Serum ◽  
Low Intensity

Abstract Chemotherapeutic efficacy plays a significant role in the development of nanotheranostic systems for drug delivery in tumor cells. In this study, we demonstrate the self-assembly of C225 conjugate, Perfluorohexane/Gold Nanoparticles (Au-PFH-NPs), which results in low-intensity focused ultrasound diagnosis ablation of thyroid cancer treatment. Cetuximab-Conjugated Perfluorohexane/Gold Nanoparticles (C-Au-PFH-NPs) showed excellent stability in water, PBS, and 20% rat serum. Transmission electron microscopy images revealed the effective construction of C-Au-PFH-NPs with commonly spherical assemblies. The incubation of C625 thyroid carcinoma with C-Au-PFH-NPs triggered apoptosis, which was confirmed by flow cytometry analysis. The C-Au-PFH-NPs showed remarkable antitumor efficacy in human thyroid carcinoma xenografts. The histopathological results additionally confirm the achieved outcomes. Furthermore, we successfully examined the efficiency of C-Au-PFH-NPs when using the thyroid carcinoma low-intensity focused ultrasound (LIFUS) diagnostic imaging in vivo. These findings are clear for LIFUS agents with high performing images. It is also identified that different therapeutic purposes will have extensive potential for future biomedical purposes.

Abstract 2482: Fate of Targeted Ultrasound Contrast Agents After Endothelial Adhesion: A Time Course Study

Circulation ◽  
2007 ◽  
Vol 116 (suppl_16) ◽  
Author(s):  
Shivani Bowry ◽  
Jianjun Wang ◽  
Sue Ottoboni ◽  
Tom Ottoboni ◽  
William R Wagner ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Ultrasound Imaging ◽  
Time Course ◽  
Time Window ◽  
Leukocyte Adhesion ◽  
Ultrasound Contrast Agents ◽  
Acoustic Properties ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging ◽  
Time Course Study

Background: Targeted microsphere (μSPH) adhesion to endothelial epitopes forms the basis for ultrasound molecular imaging. The fate of targeted μSPH, once bound to the endothelial target, is unknown. This has limited the optimization of ultrasound imaging strategies; for example, the appropriate time to commence imaging after μSPH injection is uncertain, and in part depends on the time course and duration of μSPH binding. We therefore sought to characterize the behavior of adhered μSPH as a function of time using serial observations of rat cremaster microcirculation after μSPH injection. Methods: Fluorescent nitrogen-encapsulated polymer μSPH (diameter 3.0 ± 1.4 μm) bearing control non-specific IgG (μSPH CTL ) or monoclonal antibody against VCAM (μSPH VCAM ) or ICAM (μSPH ICAM ) were prepared. Inflamed cremaster muscle of 21 anesthetized Wistar rats was exteriorized for intravital microscopy 4 hours after intrascrotal injection of TNF-α (5μg). Each rat received 1 venous injection of a single μSPH species (n=7 rats/μSPH; 10 8 μSPH/injection). An index venule was identified for serial microscopic observation every 5 min starting 10 min after μSPH injection. Results: The cumulative sum of adhered μSPH during 1 hr observation was higher for μSPH ICAM (24±11) and μSPH VCAM (18±1) than μSPH CTL (3±2, p=0.005). The number of μSPH ICAM adherent to the index venule was stable over time (10 min: 2 ± 3; 30 min: 3±2; 80 min: 3 ± 5, ANOVA p=0.9). The number of adhered μSPH VCAM trended downwards (10 min: 5±4; 30 min: 2±2; 80 min: 1±2; ANOVA p=0.08). By 1 hr, adhered μSPH appeared altered in shape; no μSPH transmigration was seen. Conclusions: Nitrogen-filled polymer μSPH targeted to leukocyte adhesion molecules adhere to inflamed endothelium. The extent of μSPH adhesion remains relatively constant over a time period relevant for imaging applications, although beyond 1hr, the μSPH appear morphologically altered and could have different acoustic properties. These data provide a useful and broad time window for effective ultrasound imaging of molecular targets. The time course of μSPH attachment to endothelium is an important consideration when devising strategies for ultrasound molecular imaging and should be characterized in a μSPH- and target- specific manner.

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