scholarly journals Ultrasound molecular imaging of breast cancer in MCF-7 orthotopic mice using gold nanoshelled poly(lactic-co-glycolic acid) nanocapsules: a novel dual-targeted ultrasound contrast agent

2018 ◽  
Vol Volume 13 ◽  
pp. 1791-1807 ◽  
Author(s):  
Li Xu ◽  
Jing Du ◽  
Caifeng Wan ◽  
Yu Zhang ◽  
Shaowei Xie ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Molecular Imaging ◽  
Contrast Agent ◽  
Glycolic Acid ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging ◽  
Mcf 7

Novel Difolate Targeting Nano-Level Ultrasound Contrast Agent for Therapy of Breast Cancer Tumor Cells

2021 ◽  
Vol 13 (7) ◽  
pp. 1295-1303
Author(s):  
Guangheng Liu ◽  
Xiangfeng Yang ◽  
Qiming Niu ◽  
Wenkui Sun
Keyword(s):  
Breast Cancer ◽  
Contrast Agent ◽  
Tumor Cells ◽  
Folate Receptor ◽  
Ultrasound Contrast Agent ◽  
Hydroxyl Group ◽  
Binding Ability ◽  
Ultrasound Contrast ◽  
Mcf 7

ABSTRACTA new type of difolate targeting nano-level ultrasound contrast agent ((folate molecule, FOL)2-TUAs) was prepared, so as to investigate its targeted binding effect with human breast cancer mammary carcinoma cells (MCF-7) in vitro. L-2-aminoadipic acid (L-2-AD) as a branch unit was inserted at the hydroxyl end of distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH to construct a tree structure. At this time, the free hydroxyl group in the distearoyl phosphatidylethanolamine (DISP)-PEG2000-COOH structure modified the FOL with the help of N-Hydroxysuccinimide/N,N'-dicyclohexylcarbodiimide (NHS/DCC). Each 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DISP-PEG2000) connected two FOLs to generate difolate targeted nanomaterials. Nano laser particle size (PS) and Zeta potential analyzer (ZPA) were applied to analyze the physical characteristics of the material such as PS and dispersion, and the enhanced development effect in vitro was detected by the ultrasonic diagnostic instrument. Besides, the targeted binding ability of the contrast agent based on this material to folate receptor (FR) overexpressing MCF-7 cells was analyzed by flow cytometry (FCM) and fluorescence microscope. In the experiment, hydrogen-1 nuclear magnetic resonance (1H NMR) demonstrated that (FOL)2-TUAs was successfully synthesized. The surface of this material was round and uniformly distributed without aggregation. According to the relative number of FOL molecules, non-targeted nano-agent (U-TUA), monofolate targeted nano-agent (FOL-TUA), and difolate targeted nano-agent ((FOL)2-TUA) were obtained. The in vitro imaging showed that different materials exhibited enhanced imaging effects in ultrasonic diagnostic equipment. FCM and fluorescence microscopy both indicated that the difolate TUA could achieve a good binding to MCF-7 cells. Most of the nano-agents were attached to the cell membrane, surrounded by red fluorophore, namely increasing the FOL content of DISP-PEG2000 chain could enhance the targeted binding ability of tumor cells.

scholarly journals Targeted ultrasound contrast agent for molecular imaging of inflammation in high-shear flow

2006 ◽  
Vol 1 (6) ◽  
pp. 259-266 ◽  
Author(s):  
A. L. Klibanov ◽  
J. J. Rychak ◽  
W. C. Yang ◽  
S. Alikhani ◽  
B. Li ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Shear Flow ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
High Shear ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound

BR55: A Lipopeptide-Based VEGFR2-Targeted Ultrasound Contrast Agent for Molecular Imaging of Angiogenesis

2010 ◽  
Vol 45 (2) ◽  
pp. 89-95 ◽  
Author(s):  
Sibylle Pochon ◽  
Isabelle Tardy ◽  
Philippe Bussat ◽  
Thierry Bettinger ◽  
Jean Brochot ◽  
...  
Keyword(s):  
Molecular Imaging ◽  
Contrast Agent ◽  
Ultrasound Contrast Agent ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound

scholarly journals Targeted ultrasound contrast agents for ultrasound molecular imaging and therapy

2015 ◽  
Vol 31 (2) ◽  
pp. 90-106 ◽  
Author(s):  
Tom van Rooij ◽  
Verya Daeichin ◽  
Ilya Skachkov ◽  
Nico de Jong ◽  
Klazina Kooiman
Keyword(s):  
Molecular Imaging ◽  
Contrast Agents ◽  
Ultrasound Contrast Agents ◽  
Ultrasound Contrast ◽  
Targeted Ultrasound ◽  
Ultrasound Molecular Imaging
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