Ligation-dependent rolling circle amplification method for ATP determination with high selectivity and sensitivity

The Analyst ◽  
2021 ◽  
Author(s):  
Mingjian Chen ◽  
Yang Li ◽  
Peng Li ◽  
Wanni Guo ◽  
Yuxin Yang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Amplification Method

An effective ATP fluorescent sensing method was developed via the coupled using of rolling circle amplification and DNAzymes, which exhibited merits including high sensitivity and specificity, simplicity in design, and potential universality.

Fluorescence aptasensor for Ochratoxin A in food samples based on hyperbranched rolling circle amplification

2015 ◽  
Vol 7 (15) ◽  
pp. 6109-6113 ◽  
Author(s):  
Ying Zhang ◽  
Linlin Yang ◽  
Cuiying Lin ◽  
Longhua Guo ◽  
Bin Qiu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Food Samples ◽  
Rolling Circle

A fluorescence aptasensor for Ochratoxin A based on hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA

2019 ◽  
Vol 11 (18) ◽  
pp. 2450-2455 ◽  
Author(s):  
Yan Xu ◽  
Yan Li ◽  
Hongkun Zhou ◽  
Xiaoping Mei ◽  
Jieyun Ye ◽  
...  
Keyword(s):  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Selective Detection ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Amplification System ◽  
Dual Amplification ◽  
T7 Exonuclease

A novel combination of rolling circle amplification and T7 exonuclease-assisted amplification to achieve highly sensitive and selective detection of microRNA molecules was developed.

Ultrasensitive colorimetric carcinoembryonic antigen biosensor based on hyperbranched rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4330-4334 ◽  
Author(s):  
Kai Liang ◽  
Shuiting Zhai ◽  
Zhidong Zhang ◽  
Xiaoyang Fu ◽  
Jingwei Shao ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle

A hyperbranched rolling circle amplification (HRCA)-based colorimetric biosensor for carcinoembryonic antigen (CEA) with high sensitivity and specificity has been developed.

Fluorescence biosensor for folate receptors in cancer cells based on terminal protection and hyperbranched rolling circle amplification

2016 ◽  
Vol 8 (32) ◽  
pp. 6231-6235 ◽  
Author(s):  
Dawei Li ◽  
Yuanzheng Ma ◽  
Ying Zhang ◽  
Zhenyu Lin
Keyword(s):  
Cancer Cells ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Fluorescence Sensor ◽  
Folate Receptors ◽  
Rolling Circle ◽  
Fluorescence Biosensor

A fluorescence sensor for folate receptors based on terminal protection and hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

Target-responsive dumbbell probe-mediated rolling circle amplification strategy for highly sensitive Hg2+ detection

RSC Advances ◽  
2014 ◽  
Vol 4 (51) ◽  
pp. 27091-27097 ◽  
Author(s):  
Qingwang Xue ◽  
Yanqin Lv ◽  
Yuanfu Zhang ◽  
Shuling Xu ◽  
Qiaoli Yue ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Highly Sensitive ◽  
Mercuric Ions ◽  
Amplification Strategy

A novel label-free amplified fluorescent sensing scheme based on target-responsive dumbbell probe-mediated rolling circle amplification (D-RCA) has been developed for sensitive and selective detection of mercuric ions.

CRISPR-Cas12a enhanced rolling circle amplification method for ultrasensitive miRNA detection

2020 ◽  
Vol 158 ◽  
pp. 105239
Author(s):  
Gong Zhang ◽  
Lei Zhang ◽  
Jingtao Tong ◽  
Xianxian Zhao ◽  
Jianlin Ren
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Amplification Method ◽  
Mirna Detection

scholarly journals Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongke Qu ◽  
Chunmei Fan ◽  
Mingjian Chen ◽  
Xiangyan Zhang ◽  
Qijia Yan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
High Specificity ◽  
Detection Methods ◽  
Research Progress ◽  
Rolling Circle ◽  
Displacement Reactions ◽  
Short Time

AbstractThe cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

A fluorescent biosensor for highly specific and ultrasensitive detection of adenosine triphosphate based on ligation-triggered branched rolling circle amplification

2019 ◽  
Vol 11 (36) ◽  
pp. 4629-4636
Author(s):  
Hongxin Jiang ◽  
Guimei Han ◽  
Yaping Xu ◽  
Junxing Li ◽  
Xiaowei Liu ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Rolling Circle Amplification ◽  
Dna Ligase ◽  
Ultrasensitive Detection ◽  
Selective Detection ◽  
Rolling Circle ◽  
T4 Dna Ligase ◽  
Fluorescent Sensing ◽  
Fluorescent Biosensor

A fluorescent sensing strategy for ultrasensitive and highly selective detection of ATP was presented by taking advantage of the exponential amplification capability of BRCA and the extreme specificity of T4 DNA ligase toward ATP.

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

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