Fluorescence aptasensor for Ochratoxin A in food samples based on hyperbranched rolling circle amplification

2015 ◽  
Vol 7 (15) ◽  
pp. 6109-6113 ◽  
Author(s):  
Ying Zhang ◽  
Linlin Yang ◽  
Cuiying Lin ◽  
Longhua Guo ◽  
Bin Qiu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Food Samples ◽  
Rolling Circle

A fluorescence aptasensor for Ochratoxin A based on hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

Ligation-dependent rolling circle amplification method for ATP determination with high selectivity and sensitivity

The Analyst ◽  
2021 ◽  
Author(s):  
Mingjian Chen ◽  
Yang Li ◽  
Peng Li ◽  
Wanni Guo ◽  
Yuxin Yang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Amplification Method

An effective ATP fluorescent sensing method was developed via the coupled using of rolling circle amplification and DNAzymes, which exhibited merits including high sensitivity and specificity, simplicity in design, and potential universality.

Ultrasensitive colorimetric carcinoembryonic antigen biosensor based on hyperbranched rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4330-4334 ◽  
Author(s):  
Kai Liang ◽  
Shuiting Zhai ◽  
Zhidong Zhang ◽  
Xiaoyang Fu ◽  
Jingwei Shao ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle

A hyperbranched rolling circle amplification (HRCA)-based colorimetric biosensor for carcinoembryonic antigen (CEA) with high sensitivity and specificity has been developed.

Fluorescence biosensor for folate receptors in cancer cells based on terminal protection and hyperbranched rolling circle amplification

2016 ◽  
Vol 8 (32) ◽  
pp. 6231-6235 ◽  
Author(s):  
Dawei Li ◽  
Yuanzheng Ma ◽  
Ying Zhang ◽  
Zhenyu Lin
Keyword(s):  
Cancer Cells ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Fluorescence Sensor ◽  
Folate Receptors ◽  
Rolling Circle ◽  
Fluorescence Biosensor

A fluorescence sensor for folate receptors based on terminal protection and hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

scholarly journals Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongke Qu ◽  
Chunmei Fan ◽  
Mingjian Chen ◽  
Xiangyan Zhang ◽  
Qijia Yan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
High Specificity ◽  
Detection Methods ◽  
Research Progress ◽  
Rolling Circle ◽  
Displacement Reactions ◽  
Short Time

AbstractThe cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA

2019 ◽  
Vol 11 (18) ◽  
pp. 2450-2455 ◽  
Author(s):  
Yan Xu ◽  
Yan Li ◽  
Hongkun Zhou ◽  
Xiaoping Mei ◽  
Jieyun Ye ◽  
...  
Keyword(s):  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Selective Detection ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Amplification System ◽  
Dual Amplification ◽  
T7 Exonuclease

A novel combination of rolling circle amplification and T7 exonuclease-assisted amplification to achieve highly sensitive and selective detection of microRNA molecules was developed.

scholarly journals DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2017
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Lujian Zhou ◽  
Jingjing Zhang ◽  
Jie Du
Keyword(s):  
Signal Amplification ◽  
Tumor Suppressor Genes ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
And Control ◽  
High Sensitivity Detection

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

scholarly journals Strategies for Isothermal Amplification of Nucleic Acids: are they Ready to be Applied in Point of Care Diagnosis of Mycosis?

2020 ◽  
Vol 11 (3) ◽  
pp. 10559-10571
Keyword(s):  
Nucleic Acid ◽  
Clinical Laboratory ◽  
Multiple Displacement Amplification ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Isothermal Amplification ◽  
Nucleic Acid Amplification ◽  
Clinical Samples ◽  
Rolling Circle ◽  
Isothermal Nucleic Acid Amplification

The early detection of invasive fungal infection (IFD) is significant in order to decrease mortality in susceptible patients. There is, therefore, a need for sensitive and specific fungal species detection assays in a clinical laboratory for early targeted therapy. The isothermal amplification method may be useful for the screening of fungal isolates, especially in resource-poor settings. Therefore, our aim was to review the isothermal nucleic acid amplification methods and their applications in fungal pathogen detection. Out of 50 reported studies, 28, 12, 6, 2, and 2 studies used the isothermal-based assays of a loop-mediated isothermal amplification (LAMP), nucleic acid sequence-based amplification (NASBA), rolling circle amplification (RCA), multiple displacement amplification (MDA) and polymerase Spiral Reaction (PSR), respectively. Thirty-two studies used clinical samples, 18 pure culture, and four environmental samples. The diagnostic accuracy of isothermal nucleic acid amplification testing for pathogenic fungal was reported as high (sensitivity 0.89–1.0 and specificity 0.63–1.0) in all studies irrespective of the sample tested. Although the isothermal-based assays showed high sensitivity and specificity in reported studies, it is still poorer than that of PCR assays. However, improving the assay to make it simpler, more effective, and inexpensive compared with newer PCR methods are still needed.

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