CRISPR-Cas12a enhanced rolling circle amplification method for ultrasensitive miRNA detection

2020 ◽  
Vol 158 ◽  
pp. 105239
Author(s):  
Gong Zhang ◽  
Lei Zhang ◽  
Jingtao Tong ◽  
Xianxian Zhao ◽  
Jianlin Ren
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Amplification Method ◽  
Mirna Detection

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

A T7 exonuclease-assisted cyclic enzymatic amplification method coupled with rolling circle amplification: a dual-amplification strategy for sensitive and selective microRNA detection

2014 ◽  
Vol 50 (13) ◽  
pp. 1576-1578 ◽  
Author(s):  
Liang Cui ◽  
Zhi Zhu ◽  
Ninghang Lin ◽  
Huimin Zhang ◽  
Zhichao Guan ◽  
...  
Keyword(s):  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Enzymatic Amplification ◽  
Amplification Method ◽  
Microrna Detection ◽  
Dual Amplification ◽  
Amplification Strategy ◽  
Excellent Selectivity ◽  
T7 Exonuclease

A T7 exonuclease-assisted cyclic enzymatic amplification method (CEAM) was combined with rolling circle amplification (RCA) to develop a RCA–CEAM dual amplification method for ultrasensitive detection of microRNA with excellent selectivity.

Rolling-Circle Amplification: Unshared Advantages in miRNA Detection

ChemBioChem ◽  
2009 ◽  
Vol 10 (8) ◽  
pp. 1289-1291 ◽  
Author(s):  
Saskia Neubacher ◽  
Christoph Arenz
Keyword(s):  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Mirna Detection

scholarly journals Rolling Circle and Loop Mediated Isothermal Amplification Strategy for Ultrasensitive miRNA Detection

Separations ◽  
2021 ◽  
Vol 8 (10) ◽  
pp. 166
Author(s):  
Zheng Cao ◽  
Xianfeng Jiang ◽  
Guizhou Xiao ◽  
Mingcheng Xu ◽  
Hui Liu ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Clinical Sample ◽  
Rolling Circle Amplification ◽  
Isothermal Amplification ◽  
Lamp Method ◽  
Rolling Circle ◽  
Loop Mediated Isothermal Amplification ◽  
Limit Of Quantitation ◽  
Mirna Detection ◽  
Amplification Strategy

Rolling circle amplification (RCA) and loop mediated isothermal amplification (LAMP) were combined to establish the rolling circle and loop mediated isothermal amplification (RC-LAMP) method for miRNA detection. With the participation of Bst 2.0 DNA Polymerase, the method enabled RCA and LAMP amplification to occur simultaneously without thermal cycling. The limit of detection of RC-LAMP was 500 amol/L, which is comparable to previously reported amplification strategies. Moreover, its upper limit of quantitation was higher and showed a stronger resistance to matrix interference. Therefore, it is possible to detect low concentrations of miRNA in samples by increasing the total RNA added. Owing to its facile detection mode and simple operation, this method has great potential in clinical sample detection.

Ligation-dependent rolling circle amplification method for ATP determination with high selectivity and sensitivity

The Analyst ◽  
2021 ◽  
Author(s):  
Mingjian Chen ◽  
Yang Li ◽  
Peng Li ◽  
Wanni Guo ◽  
Yuxin Yang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Amplification Method

An effective ATP fluorescent sensing method was developed via the coupled using of rolling circle amplification and DNAzymes, which exhibited merits including high sensitivity and specificity, simplicity in design, and potential universality.

scholarly journals Generation of long, fully modified, and serum-resistant oligonucleotides by rolling circle amplification

2015 ◽  
Vol 13 (38) ◽  
pp. 9820-9824 ◽  
Author(s):  
Marcel Hollenstein
Keyword(s):  
Rolling Circle Amplification ◽  
Single Stranded Dna ◽  
Rolling Circle ◽  
Dna Oligonucleotides ◽  
Amplification Method ◽  
Nucleoside Triphosphates

Nucleoside triphosphates modified at any level of the scaffold were shown to be compatible with the rolling circle amplification method. The combination of modified dNTPs and RCA enables the generation of long, fully modified, single-stranded DNA oligonucleotides.

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