Fluorescence biosensor for folate receptors in cancer cells based on terminal protection and hyperbranched rolling circle amplification

2016 ◽  
Vol 8 (32) ◽  
pp. 6231-6235 ◽  
Author(s):  
Dawei Li ◽  
Yuanzheng Ma ◽  
Ying Zhang ◽  
Zhenyu Lin
Keyword(s):  
Cancer Cells ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Fluorescence Sensor ◽  
Folate Receptors ◽  
Rolling Circle ◽  
Fluorescence Biosensor

A fluorescence sensor for folate receptors based on terminal protection and hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

Fluorescence aptasensor for Ochratoxin A in food samples based on hyperbranched rolling circle amplification

2015 ◽  
Vol 7 (15) ◽  
pp. 6109-6113 ◽  
Author(s):  
Ying Zhang ◽  
Linlin Yang ◽  
Cuiying Lin ◽  
Longhua Guo ◽  
Bin Qiu ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Food Samples ◽  
Rolling Circle

A fluorescence aptasensor for Ochratoxin A based on hyperbranched rolling circle amplification with high sensitivity and specificity has been developed.

Ligation-dependent rolling circle amplification method for ATP determination with high selectivity and sensitivity

The Analyst ◽  
2021 ◽  
Author(s):  
Mingjian Chen ◽  
Yang Li ◽  
Peng Li ◽  
Wanni Guo ◽  
Yuxin Yang ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Fluorescent Sensing ◽  
Amplification Method

An effective ATP fluorescent sensing method was developed via the coupled using of rolling circle amplification and DNAzymes, which exhibited merits including high sensitivity and specificity, simplicity in design, and potential universality.

Ultrasensitive colorimetric carcinoembryonic antigen biosensor based on hyperbranched rolling circle amplification

The Analyst ◽  
2014 ◽  
Vol 139 (17) ◽  
pp. 4330-4334 ◽  
Author(s):  
Kai Liang ◽  
Shuiting Zhai ◽  
Zhidong Zhang ◽  
Xiaoyang Fu ◽  
Jingwei Shao ◽  
...  
Keyword(s):  
Carcinoembryonic Antigen ◽  
Sensitivity And Specificity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle

A hyperbranched rolling circle amplification (HRCA)-based colorimetric biosensor for carcinoembryonic antigen (CEA) with high sensitivity and specificity has been developed.

scholarly journals Recent advances of fluorescent biosensors based on cyclic signal amplification technology in biomedical detection

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hongke Qu ◽  
Chunmei Fan ◽  
Mingjian Chen ◽  
Xiangyan Zhang ◽  
Qijia Yan ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
High Specificity ◽  
Detection Methods ◽  
Research Progress ◽  
Rolling Circle ◽  
Displacement Reactions ◽  
Short Time

AbstractThe cyclic signal amplification technology has been widely applied for the ultrasensitive detection of many important biomolecules, such as nucleic acids, proteins, enzymes, adenosine triphosphate (ATP), metal ions, exosome, etc. Due to their low content in the complex biological samples, traditional detection methods are insufficient to satisfy the requirements for monitoring those biomolecules. Therefore, effective and sensitive biosensors based on cyclic signal amplification technology are of great significance for the quick and simple diagnosis and treatment of diseases. Fluorescent biosensor based on cyclic signal amplification technology has become a research hotspot due to its simple operation, low cost, short time, high sensitivity and high specificity. This paper introduces several cyclic amplification methods, such as rolling circle amplification (RCA), strand displacement reactions (SDR) and enzyme-assisted amplification (EAA), and summarizes the research progress of using this technology in the detection of different biomolecules in recent years, in order to provide help for the research of more efficient and sensitive detection methods. Graphical Abstract

scholarly journals A Label-Free Fluorescent Sensor Based on the Formation of Poly(thymine)-Templated Copper Nanoparticles for the Sensitive and Selective Detection of MicroRNA from Cancer Cells

Chemosensors ◽  
2020 ◽  
Vol 8 (3) ◽  
pp. 52
Author(s):  
Qian Ma ◽  
Zhiqiang Gao ◽  
Hiranya Dayal ◽  
Sam Fong Yau Li
Keyword(s):  
Cancer Cells ◽  
Copper Nanoparticles ◽  
Fluorescent Sensor ◽  
Rolling Circle Amplification ◽  
High Specificity ◽  
Excitation Wavelength ◽  
Selective Detection ◽  
Label Free ◽  
Rolling Circle ◽  
Highly Sensitive

In this work, a simple and label-free fluorescence “off” to “on” platform was designed for the sensitive and selective detection of microRNA (miRNA) in cancer cells. This method utilized a padlock DNA-based rolling circle amplification (P-RCA) to synthesize fluorescent poly(thymine) (PolyT) which acted as a template for the synthesis of copper nanoparticles (CuNPs) within 10 minutes under mild conditions. While the repeated PolyT sequence was used as the template for CuNP synthesis, other non-PolyT parts (single strand-DNAs without the capacity to act as the template for CuNP formation) served as “smart glues” or rigid linkers to build complex nanostructures. Under the excitation wavelength of 340 nm, the synthesized CuNPs emitted strong red fluorescence effectively at 620 nm. To demonstrate the use of this method as a universal biosensor platform, lethal-7a (let-7a) miRNA was chosen as the standard target. This sensor could achieve highly sensitive and selective detection of miRNA in the presence of other homologous analogues for the combination of P-RCA with the fluorescent copper nanoparticle. Overall, this novel label-free method holds great potential in the sensitive detection of miRNA with high specificity in real samples.

A T7 exonuclease-RCA dual amplification system for high-sensitivity and high-selectivity analysis of microRNA

2019 ◽  
Vol 11 (18) ◽  
pp. 2450-2455 ◽  
Author(s):  
Yan Xu ◽  
Yan Li ◽  
Hongkun Zhou ◽  
Xiaoping Mei ◽  
Jieyun Ye ◽  
...  
Keyword(s):  
High Selectivity ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Selective Detection ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
Amplification System ◽  
Dual Amplification ◽  
T7 Exonuclease

A novel combination of rolling circle amplification and T7 exonuclease-assisted amplification to achieve highly sensitive and selective detection of microRNA molecules was developed.

scholarly journals DNAzyme-Based Target-Triggered Rolling-Circle Amplification for High Sensitivity Detection of microRNAs

Sensors ◽  
2020 ◽  
Vol 20 (7) ◽  
pp. 2017
Author(s):  
Chen Liu ◽  
Jialun Han ◽  
Lujian Zhou ◽  
Jingjing Zhang ◽  
Jie Du
Keyword(s):  
Signal Amplification ◽  
Tumor Suppressor Genes ◽  
Low Cost ◽  
Rolling Circle Amplification ◽  
High Sensitivity ◽  
Rolling Circle ◽  
Highly Sensitive ◽  
And Control ◽  
High Sensitivity Detection

MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L−1 level hence affording a simple, highly sensitive, and selective low cost detection platform.

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