scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

Soft Matter ◽  
2020 ◽  
Vol 16 (39) ◽  
pp. 8996-9001
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Phase Contrast ◽  
Imaging Technique ◽  
Phase Contrast Imaging ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Contrast Imaging ◽  
Unilamellar Vesicles ◽  
Contrast Microscopy

Bending rigidity measured with fluctuation spectroscopy is insensitive to the imaging technique: do’s and do not’s when using confocal and phase contrast imaging.

scholarly journals Fluctuation spectroscopy of giant unilamellar vesicles using confocal and phase contrast microscopy

2020 ◽  
Author(s):  
Hammad A. Faizi ◽  
Cody J. Reeves ◽  
Vasil N. Georgiev ◽  
Petia M. Vlahovska ◽  
Rumiana Dimova
Keyword(s):  
Confocal Microscopy ◽  
Phase Contrast ◽  
Phase Contrast Microscopy ◽  
Bending Rigidity ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Bilayer Membranes ◽  
Bending Modulus ◽  
Contrast Microscopy ◽  
Thermally Driven

A widely used method to measure the bending rigidity of bilayer membranes is fluctuation spectroscopy, which analyses the thermally-driven membrane undulations of giant unilamellar vesicles recorded with either phase-contrast or confocal microscopy. Here, we analyze the fluctuations of the same vesicle using both techniques and obtain consistent values for the bending modulus. We discuss the factors that may lead to discrepancies.

Dynamic behaviour of giant unilamellar vesicles induced by the uptake of [70]fullerene

2014 ◽  
Vol 50 (11) ◽  
pp. 1288-1291 ◽  
Author(s):  
Atsushi Ikeda ◽  
Tomohiro Hida ◽  
Tatsuya Iizuka ◽  
Manami Tsukamoto ◽  
Jun-ichi Kikuchi ◽  
...  
Keyword(s):  
Exchange Reaction ◽  
Lipid Membranes ◽  
Phase Contrast ◽  
Dynamic Behaviour ◽  
Phase Contrast Microscopy ◽  
Giant Unilamellar Vesicles ◽  
Unilamellar Vesicles ◽  
Contrast Microscopy

We have directly observed the processes involved in a C70-exchange reaction from γ-cyclodextrin cavities to lipid membranes using GUVs by phase contrast microscopy.

Application of X-ray phase-contrast imaging technique in the study of pyrocarbon-coated zirconia kernels

2009 ◽  
Vol 42 (5) ◽  
pp. 384-388 ◽  
Author(s):  
Y.S. Kashyap ◽  
P.S. Yadav ◽  
P.S. Sarkar ◽  
A. Agrawal ◽  
T. Roy ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Imaging Technique ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray

Quantitative phase retrieval in X-ray Zernike phase contrast microscopy

2015 ◽  
Vol 22 (4) ◽  
pp. 1056-1061 ◽  
Author(s):  
Heng Chen ◽  
Zhili Wang ◽  
Kun Gao ◽  
Qiyue Hou ◽  
Dajiang Wang ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Phase Retrieval ◽  
Materials Science ◽  
Phase Contrast Microscopy ◽  
Contrast Imaging ◽  
X Ray ◽  
Quantitative Phase ◽  
Direct Light ◽  
Contrast Microscopy ◽  
Phase Variations

In recent years, increasing attention has been devoted to X-ray phase contrast imaging, since it can provide high-contrast images by using phase variations. Among the different existing techniques, Zernike phase contrast microscopy is one of the most popular phase-sensitive techniques for investigating the fine structure of the sample at high spatial resolution. In X-ray Zernike phase contrast microscopy, the image contrast is indeed a mixture of absorption and phase contrast. Therefore, this technique just provides qualitative information on the object, which makes the interpretation of the image difficult. In this contribution, an approach is proposed for quantitative phase retrieval in X-ray Zernike phase contrast microscopy. By shifting the phase of the direct light by π/2 and 3π/2, two images of the same object are measured successively. The phase information of the object can then be quantitatively retrieved by a proper combination of the measured images. Numerical experiments were carried out and the results confirmed the feasibility of the proposed method. It is expected that the proposed method will find widespread applications in biology, materials science and so on.

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