scholarly journals Identification and enhancing production of a novel macrolide compound in engineered Streptomyces peucetius

RSC Advances ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 3168-3173
Author(s):  
Van Thuy Thi Pham ◽  
Hue Thi Nguyen ◽  
Chung Thanh Nguyen ◽  
Ye Seul Choi ◽  
Dipesh Dhakal ◽  
...  
Keyword(s):  
Streptomyces Peucetius ◽  
Enhancing Production

Activation of peucemycin in S. peucetius DM07 by the OSMAC strategy.

Exploring Genetic Diversity in Cashmere Goats of Ladakh for Enhancing Production

2021 ◽  
pp. 106409
Author(s):  
F.D. Sheikh ◽  
T.A.S. Ganai ◽  
A.M Ganai ◽  
Safeer Alam ◽  
Sabiya Asmat
Keyword(s):  
Genetic Diversity ◽  
Cashmere Goats ◽  
Enhancing Production

scholarly journals Can Appropriate Thermal Post-Treatment Make Defect Content in as-Built Electron Beam Additively Manufactured Alloy 718 Irrelevant?

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 536 ◽  
Author(s):  
Sneha Goel ◽  
Kévin Bourreau ◽  
Jonas Olsson ◽  
Uta Klement ◽  
Shrikant Joshi
Keyword(s):  
Electron Beam ◽  
Process Optimization ◽  
Electron Beam Melting ◽  
Phase Distribution ◽  
Hot Isostatic Pressing ◽  
Alloy 718 ◽  
Proof Of Concept ◽  
Defect Content ◽  
Enhancing Production ◽  
Hardness Values

Electron beam melting (EBM) is gaining rapid popularity for production of complex customized parts. For strategic applications involving materials like superalloys (e.g., Alloy 718), post-treatments including hot isostatic pressing (HIPing) to eliminate defects, and solutionizing and aging to achieve the desired phase constitution are often practiced. The present study specifically explores the ability of the combination of the above post-treatments to render the as-built defect content in EBM Alloy 718 irrelevant. Results show that HIPing can reduce defect content from as high as 17% in as-built samples (intentionally generated employing increased processing speeds in this illustrative proof-of-concept study) to <0.3%, with the small amount of remnant defects being mainly associated with oxide inclusions. The subsequent solution and aging treatments are also found to yield virtually identical phase distribution and hardness values in samples with vastly varying as-built defect contents. This can have considerable implications in contributing to minimizing elaborate process optimization efforts as well as slightly enhancing production speeds to promote industrialization of EBM for applications that demand the above post-treatments.

Amylase and cysteine proteinase gene knockdown in rice cells using RNA interference for enhancing production of recombinant proteins

2013 ◽  
Vol 114 (1) ◽  
pp. 97-107 ◽  
Author(s):  
Nan-Sun Kim ◽  
Seon-Hui Jang ◽  
Hwa-Young Yu ◽  
Nguyen-Duc Chung ◽  
Tae-Ho Kwon ◽  
...  
Keyword(s):  
Rna Interference ◽  
Recombinant Proteins ◽  
Cysteine Proteinase ◽  
Gene Knockdown ◽  
Enhancing Production

Isolation of a chitinase overproducing mutant of Streptomyces peucetius defective in daunorubicin biosynthesis

2000 ◽  
Vol 46 (10) ◽  
pp. 956-960 ◽  
Author(s):  
Kuzhandhaivel S Vetrivel ◽  
Kuppamuthu Dharmalingam
Keyword(s):  
Biosynthetic Pathway ◽  
Time Course ◽  
Polyketide Synthase ◽  
Protein Profile ◽  
Restriction Enzymes ◽  
Wild Type ◽  
Anthracycline Antibiotic ◽  
Streptomyces Peucetius ◽  
Chitinase Production ◽  
Major Alteration

Streptomyces peucetius, producer of the antitumor anthracycline antibiotic daunorubicin, was mutagenized, and mutants defective in daunorubicin biosynthesis were screened. One mutant (SPVI), which failed to produce daunorubicin, was found to overproduce an extracellular chitinase. Time course analyses of chitinase production and of the extracellular protein profile showed that the increase in activity is due to increased synthesis of the enzyme protein. The production of chitinase in SPVI was repressed by glucose as in the case of wild-type S. peucetius. PFGE analysis of VspI restriction fragments of S. peucetius and SPVI showed that there was no major alteration in the mutant genome. The hybridization pattern of S. peucetius and SPVI genomic DNA digested with various restriction enzymes was identical when probed with dnrUVJI genes of the S. peucetius daunorubicin cluster and chiA of Streptomyces lividans 66. The possible step affected in the daunorubicin biosynthetic pathway could be a polyketide synthase, since aklanonic acid, the earliest detectable intermediate in the daunorubicin pathway, was not synthesized in SPVI.Key words: Streptomyces peucetius, chitinase, daunorubicin, NTG mutagenesis.

scholarly journals Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952

2016 ◽  
Vol 192 ◽  
pp. 96-102 ◽  
Author(s):  
Anaya Raj Pokhrel ◽  
Amit Kumar Chaudhary ◽  
Hue Thi Nguyen ◽  
Dipesh Dhakal ◽  
Tuoi Thi Le ◽  
...  
Keyword(s):  
Negative Regulator ◽  
Streptomyces Peucetius

Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis

2015 ◽  
Vol 25 (5) ◽  
pp. 658-661 ◽  
Author(s):  
Gopal Prasad Ghimire ◽  
Niranjan Koirala ◽  
Jae Kyung Sohng
Keyword(s):  
Streptomyces Peucetius
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