Characterization of Dephosphocoenzyme A Kinase from Streptomyces peucetius ATCC27952, and Its Application for Doxorubicin Overproduction

2014 ◽  
Vol 24 (9) ◽  
pp. 1238-1244 ◽  
Author(s):  
Na-Rae Lee ◽  
Hemraj Rimal ◽  
Joo-Ho Lee ◽  
Tae-Jin Oh
Keyword(s):  
Streptomyces Peucetius

Characterization of CYP166B1 and its electron transfer system in Streptomyces peucetius var. caesius ATCC 27952

2010 ◽  
Vol 46 (5) ◽  
pp. 372-377 ◽  
Author(s):  
Pramod Shrestha ◽  
Tae-Jin Oh ◽  
Narayan Prasad Niraula ◽  
Kwangkyoung Liou ◽  
Jin Cheol Yoo ◽  
...  
Keyword(s):  
Electron Transfer ◽  
Transfer System ◽  
Streptomyces Peucetius ◽  
Electron Transfer System

Biochemical characterization of the glucose kinase from Streptomyces coelicolor compared to Streptomyces peucetius var. caesius

2005 ◽  
Vol 156 (3) ◽  
pp. 361-366 ◽  
Author(s):  
Iveta Imriskova ◽  
Roberto Arreguín-Espinosa ◽  
Silvia Guzmán ◽  
Romina Rodriguez-Sanoja ◽  
Elizabeth Langley ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Streptomyces Coelicolor ◽  
Streptomyces Peucetius ◽  
Glucose Kinase

scholarly journals Doxorubicin Overproduction in Streptomyces peucetius: Cloning and Characterization of the dnrU Ketoreductase anddnrV Genes and the doxA Cytochrome P-450 Hydroxylase Gene

1999 ◽  
Vol 181 (1) ◽  
pp. 305-318 ◽  
Author(s):  
Natalie Lomovskaya ◽  
Sharee L. Otten ◽  
Yukiko Doi-Katayama ◽  
Leonid Fonstein ◽  
Xiao-Chun Liu ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Double Mutant ◽  
Polyketide Synthase ◽  
Enzymatic Reactions ◽  
Triple Mutant ◽  
Streptomyces Peucetius ◽  
Polyketide Synthase Gene ◽  
Notable Increase ◽  
Cytochrome P 450

Doxorubicin-overproducing strains of Streptomyces peucetius ATCC 29050 can be obtained through manipulation of the genes in the region of the doxorubicin (DXR) gene cluster that containsdpsH, the dpsG polyketide synthase gene, the putative dnrU ketoreductase gene, dnrV, and thedoxA cytochrome P-450 gene. These five genes were characterized by sequence analysis, and the effects of replacingdnrU, dnrV, doxA, ordpsH with mutant alleles and of doxAoverexpression on the production of the principal anthracycline metabolites of S. peucetius were studied. The exact roles of dpsH and dnrV could not be established, although dnrV is implicated in the enzymatic reactions catalyzed by DoxA, but dnrU appears to encode a ketoreductase specific for the C-13 carbonyl of daunorubicin (DNR) and DXR or their biosynthetic precursors. The highest DXR titers were obtained in a dnrX dnrU (N. Lomovskaya, Y. Doi-Katayama, S. Filippini, C. Nastro, L. Fonstein, M. Gallo, A. L. Colombo, and C. R. Hutchinson, J. Bacteriol. 180:2379–2386, 1998) double mutant and a dnrX dnrU dnrH (C. Scotti and C. R. Hutchinson, J. Bacteriol. 178:7316–7321, 1996) triple mutant. Overexpression of doxA in adoxA::aphII mutant resulted in the accumulation of DXR precursors instead of in a notable increase in DXR production. In contrast, overexpression of dnrV and doxAjointly in the dnrX dnrU double mutant or the dnrX dnrU dnrH triple mutant increased the DXR titer 36 to 86%.

scholarly journals EXPRESSION, PURIFICATION AND CHARACTERIZATION OF CHITINASE CHIC PRECURSOR FROM Streptomyces peucetius

2018 ◽  
Vol 6 (4) ◽  
pp. 669-676
Author(s):  
Amrathlal Rabbind Singh ◽  
◽  
Chitra Thangavel ◽  
Kuppamuthu Dharmalingam ◽  
◽  
...  
Keyword(s):  
Purification And Characterization ◽  
Streptomyces Peucetius
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