Nomenclature Abstract for Streptomyces peucetius Grein et al. 1963 (Approved Lists 1980).

2003 ◽  
Author(s):  
Charles Thomas Parker ◽  
George M Garrity
Keyword(s):  
Streptomyces Peucetius

Isolation of a chitinase overproducing mutant of Streptomyces peucetius defective in daunorubicin biosynthesis

2000 ◽  
Vol 46 (10) ◽  
pp. 956-960 ◽  
Author(s):  
Kuzhandhaivel S Vetrivel ◽  
Kuppamuthu Dharmalingam
Keyword(s):  
Biosynthetic Pathway ◽  
Time Course ◽  
Polyketide Synthase ◽  
Protein Profile ◽  
Restriction Enzymes ◽  
Wild Type ◽  
Anthracycline Antibiotic ◽  
Streptomyces Peucetius ◽  
Chitinase Production ◽  
Major Alteration

Streptomyces peucetius, producer of the antitumor anthracycline antibiotic daunorubicin, was mutagenized, and mutants defective in daunorubicin biosynthesis were screened. One mutant (SPVI), which failed to produce daunorubicin, was found to overproduce an extracellular chitinase. Time course analyses of chitinase production and of the extracellular protein profile showed that the increase in activity is due to increased synthesis of the enzyme protein. The production of chitinase in SPVI was repressed by glucose as in the case of wild-type S. peucetius. PFGE analysis of VspI restriction fragments of S. peucetius and SPVI showed that there was no major alteration in the mutant genome. The hybridization pattern of S. peucetius and SPVI genomic DNA digested with various restriction enzymes was identical when probed with dnrUVJI genes of the S. peucetius daunorubicin cluster and chiA of Streptomyces lividans 66. The possible step affected in the daunorubicin biosynthetic pathway could be a polyketide synthase, since aklanonic acid, the earliest detectable intermediate in the daunorubicin pathway, was not synthesized in SPVI.Key words: Streptomyces peucetius, chitinase, daunorubicin, NTG mutagenesis.

scholarly journals Overexpression of a pathway specific negative regulator enhances production of daunorubicin in bldA deficient Streptomyces peucetius ATCC 27952

2016 ◽  
Vol 192 ◽  
pp. 96-102 ◽  
Author(s):  
Anaya Raj Pokhrel ◽  
Amit Kumar Chaudhary ◽  
Hue Thi Nguyen ◽  
Dipesh Dhakal ◽  
Tuoi Thi Le ◽  
...  
Keyword(s):  
Negative Regulator ◽  
Streptomyces Peucetius

Activation of Cryptic hop Genes from Streptomyces peucetius ATCC 27952 Involved in Hopanoid Biosynthesis

2015 ◽  
Vol 25 (5) ◽  
pp. 658-661 ◽  
Author(s):  
Gopal Prasad Ghimire ◽  
Niranjan Koirala ◽  
Jae Kyung Sohng
Keyword(s):  
Streptomyces Peucetius

scholarly journals The Streptomyces peucetius drrC gene encodes a UvrA-like protein involved in daunorubicin resistance and production.

1996 ◽  
Vol 178 (11) ◽  
pp. 3238-3245 ◽  
Author(s):  
N Lomovskaya ◽  
S K Hong ◽  
S U Kim ◽  
L Fonstein ◽  
K Furuya ◽  
...  
Keyword(s):  
Streptomyces Peucetius

scholarly journals ChiS histidine kinase negatively regulates the production of chitinase ChiC in Streptomyces peucetius

2014 ◽  
Vol 169 (2-3) ◽  
pp. 155-162 ◽  
Author(s):  
Amrathlal Rabbind Singh ◽  
Paranthaman Senthamaraikannan ◽  
Chitra Thangavel ◽  
Ravikanth Danda ◽  
Shunmugiah Karutha Pandian ◽  
...  
Keyword(s):  
Histidine Kinase ◽  
Streptomyces Peucetius

scholarly journals Improved Squalene Production via Modulation of the Methylerythritol 4-Phosphate Pathway and Heterologous Expression of Genes from Streptomyces peucetius ATCC 27952 in Escherichia coli

2009 ◽  
Vol 75 (22) ◽  
pp. 7291-7293 ◽  
Author(s):  
Gopal Prasad Ghimire ◽  
Hei Chan Lee ◽  
Jae Kyung Sohng
Keyword(s):  
Escherichia Coli ◽  
Heterologous Expression ◽  
Farnesyl Diphosphate ◽  
Farnesyl Diphosphate Synthase ◽  
Isopentenyl Diphosphate ◽  
Isopentenyl Diphosphate Isomerase ◽  
Streptomyces Peucetius ◽  
E Coli ◽  
Expression Of Genes ◽  
Genes Encoding

ABSTRACT Putative hopanoid genes from Streptomyces peucetius were introduced into Escherichia coli to improve the production of squalene, an industrially important compound. High expression of hopA and hopB (encoding squalene/phytoene synthases) together with hopD (encoding farnesyl diphosphate synthase) yielded 4.1 mg/liter of squalene. This level was elevated to 11.8 mg/liter when there was also increased expression of dxs and idi, E. coli genes encoding 1-deoxy-d-xylulose 5-phosphate synthase and isopentenyl diphosphate isomerase.

scholarly journals Streptomyces peucetius daunorubicin biosynthesis gene, dnrF: sequence and heterologous expression

Microbiology ◽  
1995 ◽  
Vol 141 (4) ◽  
pp. 1007-1016 ◽  
Author(s):  
S. Filippini ◽  
M. M. Solinas ◽  
U. Breme ◽  
M. B. Schluter ◽  
D. Gabellini ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Streptomyces Peucetius ◽  
Biosynthesis Gene
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