scholarly journals Cell-loaded injectable gelatin/alginate/LAPONITE® nanocomposite hydrogel promotes bone healing in a critical-size rat calvarial defect model

RSC Advances ◽  
2020 ◽  
Vol 10 (43) ◽  
pp. 25652-25661
Author(s):  
Bin Liu ◽  
Junqin Li ◽  
Xing Lei ◽  
Sheng Miao ◽  
Shuaishuai Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bone Healing ◽  
Critical Size ◽  
Nanocomposite Hydrogel ◽  
Calvarial Defect ◽  
Defect Model ◽  
Promote Cell Proliferation ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

An injectable cell-laden nanocomposite hydrogel simulate natural ECM, promote cell proliferation, and accelerate bone healing of critical-size rat calvarial defects.

scholarly journals The bone-healing effect of a xenograft in a rat calvarial defect model

2009 ◽  
Vol 28 (4) ◽  
pp. 396-400 ◽  
Author(s):  
Hakan DEVELIOGLU ◽  
Serpil UNVER SARAYDIN ◽  
Unal KARTAL
Keyword(s):  
Bone Healing ◽  
Calvarial Defect ◽  
Defect Model ◽  
Healing Effect ◽  
Rat Calvarial Defect

Nanohydroxyapatite/cellulose nanocrystals/silk fibroin ternary scaffolds for rat calvarial defect regeneration

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35684-35691 ◽  
Author(s):  
Xiaoming Chen ◽  
Runmei Zhou ◽  
Bin Chen ◽  
Jianting Chen
Keyword(s):  
Silk Fibroin ◽  
Cellulose Nanocrystals ◽  
Critical Size ◽  
Calvarial Defect ◽  
Calvarial Defects ◽  
Biomimetic Scaffold ◽  
Rat Calvarial Defect

The purpose of this study was to design and characterise a novel biomimetic scaffold for the repair of critical size calvarial defects.

Efficacy and safety of a novel hemostatic material, BoneStat, compared with Ostene and Bone Wax in a rat calvarial defect model

2021 ◽  
pp. 039139882110214
Author(s):  
Seon Young Choi ◽  
Jiheon Rhim ◽  
Seon A Heo ◽  
Woo-Jung Han ◽  
Myung Hee Kim ◽  
...  
Keyword(s):  
Bone Healing ◽  
Water Solubility ◽  
Water Soluble ◽  
Calvarial Defect ◽  
Defect Model ◽  
Inflammatory Reactions ◽  
Alkylene Oxide ◽  
Rat Calvarial Defect ◽  
Inflammation Reaction ◽  
Consequent Increase

Hemostasis has critical significance during surgical procedures. Bone Wax has traditionally been commonly used for bone hemostasis despite well-documented undesirable side effects: hindering osteogenesis and induction of inflammatory reactions with consequent increase in infection rates. A later developed formulation, Ostene, offers an alternative to Bone Wax with lesser undesired effects. In this study, BoneStat, a newly developed bone hemostatic formulation comprising water-soluble alkylene oxide co-polymers, was evaluated for water solubility, hemostatic efficacy, ease of handling, bone healing efficacy, and inflammatory reactions compared with Bone Wax and Ostene in a rat calvarial defect model. More than 95% of BoneStat was dissolved in water within 48 h, as was Ostene, but not Bone Wax. The time to hemostasis using BoneStat was significantly faster than with Ostene or Bone Wax. BoneStat also improved ease of handling compared to Ostene or BoneWax. BoneStat- and Ostene-treated groups constantly showed better bone healing than with Bone Wax. The BoneStat and Ostene groups presented no evidence of chronic inflammation reaction contrary to Bone Wax. These results suggest improved hemostasis, ease of handling, non-hindering bone healing, and unnoticeable chronic inflammatory reactions with BoneStat. Thus, Bonestat is a useful and reliable formulation for mechanical hemostasis.

scholarly journals Canine Mesenchymal Stromal Cell-Mediated Bone Regeneration is Enhanced in the Presence of Sub-Therapeutic Concentrations of BMP-2 in a Murine Calvarial Defect Model

2021 ◽  
Vol 9 ◽  
Author(s):  
Lauren K. Dobson ◽  
Suzanne Zeitouni ◽  
Eoin P. McNeill ◽  
Robert N. Bearden ◽  
Carl A. Gregory ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Healing ◽  
Human Mscs ◽  
Calvarial Defect ◽  
Defect Model ◽  
Alp Activity ◽  
Calvarial Defects ◽  
Species Specific

Novel bone regeneration strategies often show promise in rodent models yet are unable to successfully translate to clinical therapy. Sheep, goats, and dogs are used as translational models in preparation for human clinical trials. While human MSCs (hMSCs) undergo osteogenesis in response to well-defined protocols, canine MSCs (cMSCs) are more incompletely characterized. Prior work suggests that cMSCs require additional agonists such as IGF-1, NELL-1, or BMP-2 to undergo robust osteogenic differentiation in vitro. When compared directly to hMSCs, cMSCs perform poorly in vivo. Thus, from both mechanistic and clinical perspectives, cMSC and hMSC-mediated bone regeneration may differ. The objectives of this study were twofold. The first was to determine if previous in vitro findings regarding cMSC osteogenesis were substantiated in vivo using an established murine calvarial defect model. The second was to assess in vitro ALP activity and endogenous BMP-2 gene expression in both canine and human MSCs. Calvarial defects (4 mm) were treated with cMSCs, sub-therapeutic BMP-2, or the combination of cMSCs and sub-therapeutic BMP-2. At 28 days, while there was increased healing in defects treated with cMSCs, defects treated with cMSCs and BMP-2 exhibited the greatest degree of bone healing as determined by quantitative μCT and histology. Using species-specific qPCR, cMSCs were not detected in relevant numbers 10 days after implantation, suggesting that bone healing was mediated by anabolic cMSC or ECM-driven cues and not via engraftment of cMSCs. In support of this finding, defects treated with cMSC + BMP-2 exhibited robust deposition of Collagens I, III, and VI using immunofluorescence. Importantly, cMSCs exhibited minimal ALP activity unless cultured in the presence of BMP-2 and did not express endogenous canine BMP-2 under any condition. In contrast, human MSCs exhibited robust ALP activity in all conditions and expressed human BMP-2 when cultured in control and osteoinduction media. This is the first in vivo study in support of previous in vitro findings regarding cMSC osteogenesis, namely that cMSCs require additional agonists to initiate robust osteogenesis. These findings are highly relevant to translational cell-based bone healing studies and represent an important finding for the field of canine MSC-mediated bone regeneration.

scholarly journals Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1521
Author(s):  
Zhe Xing ◽  
Xiaofeng Jiang ◽  
Qingzong Si ◽  
Anna Finne-Wistrand ◽  
Bin Liu ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Host Response ◽  
Endochondral Ossification ◽  
Marrow Stromal Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Calvarial Defects ◽  
Ossification Process ◽  
Rat Calvarial Defect

It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

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