Nanohydroxyapatite/cellulose nanocrystals/silk fibroin ternary scaffolds for rat calvarial defect regeneration

RSC Advances ◽  
2016 ◽  
Vol 6 (42) ◽  
pp. 35684-35691 ◽  
Author(s):  
Xiaoming Chen ◽  
Runmei Zhou ◽  
Bin Chen ◽  
Jianting Chen
Keyword(s):  
Silk Fibroin ◽  
Cellulose Nanocrystals ◽  
Critical Size ◽  
Calvarial Defect ◽  
Calvarial Defects ◽  
Biomimetic Scaffold ◽  
Rat Calvarial Defect

The purpose of this study was to design and characterise a novel biomimetic scaffold for the repair of critical size calvarial defects.

scholarly journals Cell-loaded injectable gelatin/alginate/LAPONITE® nanocomposite hydrogel promotes bone healing in a critical-size rat calvarial defect model

RSC Advances ◽  
2020 ◽  
Vol 10 (43) ◽  
pp. 25652-25661
Author(s):  
Bin Liu ◽  
Junqin Li ◽  
Xing Lei ◽  
Sheng Miao ◽  
Shuaishuai Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bone Healing ◽  
Critical Size ◽  
Nanocomposite Hydrogel ◽  
Calvarial Defect ◽  
Defect Model ◽  
Promote Cell Proliferation ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

An injectable cell-laden nanocomposite hydrogel simulate natural ECM, promote cell proliferation, and accelerate bone healing of critical-size rat calvarial defects.

scholarly journals The effect of silk fibroin and rhBMP-2 on bone regeneration in rat calvarial defect model

2010 ◽  
Vol 36 (5) ◽  
pp. 366 ◽  
Author(s):  
Jeong-Hun Nam ◽  
Kyung-Lok Noh ◽  
Eun-O Pang ◽  
Woo-Geun Yu ◽  
Eung-Sun Kang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Silk Fibroin ◽  
Calvarial Defect ◽  
Defect Model ◽  
Rat Calvarial Defect

scholarly journals Evaluation of Open Hollow Hydroxyapatite Microsphere on Bone Regeneration in Rat Calvarial Defects

2019 ◽  
Author(s):  
Youqu Shen ◽  
Mohamed Rahaman ◽  
Yongxian Liu ◽  
Yue-Wern Huang
Keyword(s):  
Surface Roughness ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Convex Surface ◽  
Total Surface Area ◽  
Calvarial Defect ◽  
New Bone Formation ◽  
Calvarial Defects ◽  
The Difference ◽  
Rat Calvarial Defect

AbstractHollow hydroxyapatite (HA) microspheres showed the ability to facilitate bone regeneration in rats with non-healing calvarial defects. However, new bone formation in the rat calvarial defect implanted with the closed HA microspheres was limited. The objective of this work is to evaluate size-, time, and structure-dependent bone regeneration between open and closed HA microspheres in an osseous model. Open HA microspheres were obtained by sectioning closed HA microspheres. The open HA microsphere had dense convex surface and rough and porous concave surface. For both size ranges (ϕ106-150 μm vs. ϕ212-250 μm), the open HA microsphere were more effective in facilitating bone regeneration than the closed HA microspheres in rat calvarial defects. Bone regeneration in the open HA microspheres (49 ± 7% for ϕ106-150 μm; 40 ± 8% for ϕ212-250 μm) were higher than the closed HA microsphere (26 ± 8% for ϕ106-150 μm; 30 ± 9% for ϕ212-250 μm) at 12 weeks. Furthermore, the open HA microspheres of smaller size showed a significant increase in bone regeneration than the open HA microspheres of larger size at both 6 weeks and 12 weeks. The difference in bone regeneration between these microspheres could be due to their differences in microstructures, namely curvature, concavity, porosity, surface roughness, and total surface area available for cells to attached to.

scholarly journals Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1521
Author(s):  
Zhe Xing ◽  
Xiaofeng Jiang ◽  
Qingzong Si ◽  
Anna Finne-Wistrand ◽  
Bin Liu ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Host Response ◽  
Endochondral Ossification ◽  
Marrow Stromal Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Calvarial Defects ◽  
Ossification Process ◽  
Rat Calvarial Defect

It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

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