scholarly journals Endochondral Ossification Induced by Cell Transplantation of Endothelial Cells and Bone Marrow Stromal Cells with Copolymer Scaffold Using a Rat Calvarial Defect Model

Polymers ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 1521
Author(s):  
Zhe Xing ◽  
Xiaofeng Jiang ◽  
Qingzong Si ◽  
Anna Finne-Wistrand ◽  
Bin Liu ◽  
...  
Keyword(s):  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Host Response ◽  
Endochondral Ossification ◽  
Marrow Stromal Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Calvarial Defects ◽  
Ossification Process ◽  
Rat Calvarial Defect

It has been recently reported that, in a rat calvarial defect model, adding endothelial cells (ECs) to a culture of bone marrow stromal cells (BMSCs) significantly enhanced bone formation. The aim of this study is to further investigate the ossification process of newly formed osteoid and host response to the poly(L-lactide-co-1,5-dioxepan-2-one) [poly(LLA-co-DXO)] scaffolds based on previous research. Several different histological methods and a PCR Array were applied to evaluate newly formed osteoid after 8 weeks after implantation. Histological results showed osteoid formed in rat calvarial defects and endochondral ossification-related genes, such as dentin matrix acidic phosphoprotein 1 (Dmp1) and collagen type II, and alpha 1 (Col2a1) exhibited greater expression in the CO (implantation with BMSC/EC/Scaffold constructs) than the BMSC group (implantation with BMSC/Scaffold constructs) as demonstrated by PCR Array. It was important to notice that cartilage-like tissue formed in the pores of the copolymer scaffolds. In addition, multinucleated giant cells (MNGCs) were observed surrounding the scaffold fragments. It was concluded that the mechanism of ossification might be an endochondral ossification process when the copolymer scaffolds loaded with co-cultured ECs/BMSCs were implanted into rat calvarial defects. MNGCs were induced by the poly(LLA-co-DXO) scaffolds after implantation, and more specific in vivo studies are needed to gain a better understanding of host response to copolymer scaffolds.

Comparison among Four Kinds of Bone Tissue Engineering Scaffold

2007 ◽  
Vol 330-332 ◽  
pp. 963-966 ◽  
Author(s):  
Lei Liu ◽  
Run Liang Chen ◽  
Yun Feng Lin ◽  
Cai Li ◽  
Wei Dong Tian ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Defects ◽  
Marrow Stromal Cells ◽  
Autogenous Bone ◽  
Calvarial Defects ◽  
Reconstruction Of Bone Defects

Bone tissue engineering is a promising way to repair of bone defects. To choose a proper scaffold is still a disputable problem in bone tissue engineering. This study aimed to compare the effects of repairing critical calvarial defects with the compounds of autogenous bone marrow stromal cells (BMSCs) and coral hydroxyapatite(CHA), hydroxyapatite/ tricalcium phosphate (HA/TCP), poly(lactide-co-glycolide) (PLGA) and alginate (AG). The results showed that CHA and AG were satisfactory bone tissues engineering scaffolds among the four kinds of materials. BMSCs/CHA and BMSCs/AG are promising techniques for reconstruction of bone defects.

scholarly journals Cell-loaded injectable gelatin/alginate/LAPONITE® nanocomposite hydrogel promotes bone healing in a critical-size rat calvarial defect model

RSC Advances ◽  
2020 ◽  
Vol 10 (43) ◽  
pp. 25652-25661
Author(s):  
Bin Liu ◽  
Junqin Li ◽  
Xing Lei ◽  
Sheng Miao ◽  
Shuaishuai Zhang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Bone Healing ◽  
Critical Size ◽  
Nanocomposite Hydrogel ◽  
Calvarial Defect ◽  
Defect Model ◽  
Promote Cell Proliferation ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

An injectable cell-laden nanocomposite hydrogel simulate natural ECM, promote cell proliferation, and accelerate bone healing of critical-size rat calvarial defects.

Dimethyloxallyl Glycine-Incorporated Borosilicate Bioactive Glass Scaffolds for Improving Angiogenesis and Osteogenesis in Critical-Sized Calvarial Defects

2019 ◽  
Vol 16 (6) ◽  
pp. 565-576 ◽  
Author(s):  
Xiangyun Jin ◽  
Dan Han ◽  
Jie Tao ◽  
Yinjun Huang ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
In Vivo Studies ◽  
New Bone Formation ◽  
Calvarial Bone ◽  
Calvarial Defects

Background: In the field of bone tissue engineering, there has been an increasing interest in biomedical materials with both high angiogenic ability and osteogenic ability. Among various osteogenesis materials, bioactive borosilicate and borate glass scaffolds possess suitable degradation rate and mechanical strength, thus drawing many scholars’ interests and attention. Objective: In this study, we fabricated bioactive glass scaffolds composed of borosilicate 2B6Sr using the Template-Method and incorporated Dimethyloxalylglycine (DMOG), a small-molecule angiogenic drug possessing good angiogenic ability, to improve bone regeneration. Methods: The in-vitro studies showed that porous borosilicate bioactive glass scaffolds released slowly, a steady amount of DMOG and stimulated the proliferation and osteogenic differentiation of human bone marrow stromal cells hBMSCs. Results: In-vivo studies showed that the borosilicate bioactive glass scaffolds could significantly promote new bone formation and neovascularization in rats’ calvarial bone defects. Conclusion: These results indicated that DMOG-incorporated bioactive glass scaffold is a successful compound with excellent angiogenesis-osteogenesis ability, which has favorable clinical prospects.

scholarly journals Interleukin-1β modulates endochondral ossification by human adult bone marrow stromal cells

2012 ◽  
Vol 24 ◽  
pp. 224-236 ◽  
Author(s):  
M Mumme ◽  
◽  
C Scotti ◽  
A Papadimitropoulos ◽  
A Todorov ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Endochondral Ossification ◽  
Marrow Stromal Cells ◽  
Interleukin 1Β ◽  
Adult Bone Marrow ◽  
Human Adult ◽  
Adult Bone

Transplanted Endothelial Cells Enhance Orthotopic Bone Regeneration

2006 ◽  
Vol 85 (7) ◽  
pp. 633-637 ◽  
Author(s):  
D. Kaigler ◽  
P.H. Krebsbach ◽  
Z. Wang ◽  
E.R. West ◽  
K. Horger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Matrix ◽  
Marrow Stromal Cells ◽  
Calvarial Defects ◽  
Osseous Defect

The aim of this study was to determine if endothelial cells could enhance bone marrow stromal-cell-mediated bone regeneration in an osseous defect. Using poly-lactide-co-glycolide scaffolds as cell carriers, we transplanted bone marrow stromal cells alone or with endothelial cells into 8.5-mm calvarial defects created in nude rats. Histological analyses of blood vessel and bone formation were performed, and microcomputed tomography (μCT) was used to assess mineralized bone matrix. Though the magnitude of the angiogenic response between groups was the same, μCT analysis revealed earlier mineralization of bone in the co-transplantation condition. Ultimately, there was a significant increase (40%) in bone formation in the co-transplantation group (33 ± 2%), compared with the transplantation of bone marrow stromal cells alone (23 ± 3%). Analysis of these data demonstrates that, in an orthotopic site, transplanted endothelial cells can influence the bone-regenerative capacity of bone marrow stromal cells.

scholarly journals The Bone Regeneration Using Bone Marrow Stromal Cells with Moderate Concentration Platelet-Rich Plasma in Femoral Segmental Defect of Rats

2017 ◽  
Vol 11 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Junichi Yamakawa ◽  
Junichi Hashimoto ◽  
Mitsuo Takano ◽  
Michiaki Takagi
Keyword(s):  
Bone Marrow ◽  
Cell Proliferation ◽  
Bone Formation ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Platelet Rich Plasma ◽  
Marrow Stromal Cells ◽  
Segmental Defect ◽  
Defect Model

Background: Platelet-rich plasma (PRP) can provide an assortment of growth factors, but how PRP effects bone regeneration is still unknown. The aim of the study was to explore an optimal method of using PRP and bone marrow stromal cells (BMSCs). Methods: An in vitro experiment was first conducted to determine an appropriate quantity of PRP. BMSCs were cultured with PRP of different concentrations to assess cell proliferation and osteogenic differentiation. Following the in vitro study, a rat femoral segmental defect model was used. Five collagen mixtures consisting of different concentrations of PRP and BMSCs were prepared as follows, i) BMSCs and PRP (platelet 20 x 104/µl), ii) BMSCs and PRP (platelet 100 x 104/µl), iii) BMSCs and PRP (platelet 500 x 104/µl), iv) BMSCs, and v) PRP group (platelet 100 x 104/µl), were used to fill defect. New bone formation was evaluated by soft X-ray and histologic analyses were performed at 2, 4, 6 and 8 weeks postoperatively. Results: The cell proliferation increased PRP concentration-dependently. Cellular alkaline phosphatase activity was higher in moderate concentration than high or low concentration group’s in vitro study. In vivo study, the bone fill percentage of newly formed bone in BMSCs and PRP (platelet 100 x 104/µl) was 46.9% at 8 weeks and increased significantly compared with other groups. Conclusion: BMSCs with moderate level of PRP significantly enhanced bone formation in comparison with BMSCs or PRP transplant in a rat femoral defect model.

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