An endoplasmic reticulum-targeting fluorescent probe for imaging ˙OH in living cells

2020 ◽  
Vol 56 (47) ◽  
pp. 6344-6347 ◽  
Author(s):  
Yanyan Zhao ◽  
Hongyu Li ◽  
Ziyin Chai ◽  
Wen Shi ◽  
Xiaohua Li ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Fluorescent Probe ◽  
Lipid Droplet ◽  
Droplet Formation ◽  
Living Cells ◽  
Lipid Droplet Formation ◽  
Er Targeting ◽  
Endoplasmic Reticulum Targeting

A new ER-targeting fluorescent probe for ˙OH is developed and applied to imaging ˙OH generation as well as lipid droplet formation in ER stress.

An endoplasmic reticulum-targeting fluorescent probe for the imaging of GSH in living cells

2019 ◽  
Vol 11 (29) ◽  
pp. 3736-3740 ◽  
Author(s):  
Cheng-Shi Jiang ◽  
Zhi-Qiang Cheng ◽  
Yong-Xi Ge ◽  
Jia-Li Song ◽  
Juan Zhang ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Er Targeting ◽  
Endoplasmic Reticulum Targeting

A new endoplasmic reticulum (ER)-targeting fluorescent probe (ER-G) was designed and applied for the detection of cellular GSH in the ER.

The development of an endoplasmic reticulum-targeting fluorescent probe for the imaging of 1,4-dithiothreitol (DTT) in living cells

2021 ◽  
Author(s):  
Abdul Hadi Mehmood ◽  
Baoli Dong ◽  
Yaru Lu ◽  
Wenhui Song ◽  
Yaru Sun ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Turn On ◽  
Er Targeting ◽  
Endoplasmic Reticulum Targeting ◽  
First Time

An effective turn-on ER-targeting fluorescent probe ER-DTT was designed to image DTT of endoplasmic reticulum for the first time.

scholarly journals Induction of Liver Steatosis and Lipid Droplet Formation in ATF6α-Knockout Mice Burdened with Pharmacological Endoplasmic Reticulum Stress

2010 ◽  
Vol 21 (17) ◽  
pp. 2975-2986 ◽  
Author(s):  
Keisuke Yamamoto ◽  
Kazuna Takahara ◽  
Seiichi Oyadomari ◽  
Tetsuya Okada ◽  
Takashi Sato ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Er Stress ◽  
Lipid Droplet ◽  
Knockout Mice ◽  
Neutral Lipids ◽  
Liver Steatosis ◽  
Droplet Formation ◽  
Mrna Levels ◽  
Lipid Droplet Formation ◽  
Transcriptional Induction

Accumulation of unfolded proteins in the endoplasmic reticulum (ER) activates homeostatic responses collectively termed the unfolded protein response. Among the three principal signaling pathways operating in mammals, activating transcription factor (ATF)6α plays a pivotal role in transcriptional induction of ER-localized molecular chaperones and folding enzymes as well as components of ER-associated degradation, and thereby mouse embryonic fibroblasts deficient in ATF6α are sensitive to ER stress. However, ATF6α-knockout mice show no apparent phenotype under normal growing conditions. In this report, we burdened mice with intraperitoneal injection of the ER stress-inducing reagent tunicamycin and found that wild-type mice were able to recover from the insult, whereas ATF6α-knockout mice exhibited liver dysfunction and steatosis. Thus, ATF6α-knockout mice accumulated neutral lipids in the liver such as triacylglycerol and cholesterol, which was ascribable to blockage of β-oxidation of fatty acids caused by decreased mRNA levels of the enzymes involved in the process, suppression of very-low-density lipoprotein formation due to destabilized apolipoprotein B-100, and stimulation of lipid droplet formation resulting from transcriptional induction of adipose differentiation-related protein. Accordingly, the hepatocytes of tunicamycin-injected knockout mice were filled with many lipid droplets. These results establish links among ER stress, lipid metabolism, and steatosis.

An endoplasmic reticulum-targeting and ratiometric fluorescent probe for hypochlorous acid in living cells based on a 1,8-naphthalimide derivative

2020 ◽  
Vol 44 (42) ◽  
pp. 18389-18398
Author(s):  
Qiujuan Ma ◽  
Chunyan Wang ◽  
Guojiang Mao ◽  
Meiju Tian ◽  
Jingguo Sun ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Endoplasmic Reticulum Targeting ◽  
Ratiometric Fluorescent Probe

A novel reticulum-targeting and ratiometric fluorescent probe for determining hypochlorous acid has been developed.

A New Endoplasmic Reticulum (ER)-Targeting Fluorescent Probe for the Imaging of Cysteine in Living Cells

2020 ◽  
Vol 30 (6) ◽  
pp. 1357-1364
Author(s):  
Lei Zhou ◽  
Yunxia Li ◽  
Aiqin Zhou ◽  
Guanghui Zhang ◽  
Zhi-Qiang Cheng ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Er Targeting

A two-photon endoplasmic reticulum-targeting fluorescent probe for the imaging of pH in living cells and zebrafish

2018 ◽  
Vol 10 (47) ◽  
pp. 5702-5706 ◽  
Author(s):  
Nan Zhang ◽  
Baoli Dong ◽  
Xiuqi Kong ◽  
Wenhui Song ◽  
Weiying Lin
Keyword(s):  
Endoplasmic Reticulum ◽  
Fluorescent Probe ◽  
Living Cells ◽  
Ph Changes ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

The pH changes of the endoplasmic reticulum (ER) are closely related to many diseases.

Development of an endoplasmic reticulum-targeting fluorescent probe for the two-photon imaging of hypochlorous acid (HClO) in living cells

2019 ◽  
Vol 11 (35) ◽  
pp. 4450-4455 ◽  
Author(s):  
Wenhui Song ◽  
Baoli Dong ◽  
Yaru Lu ◽  
Xiuqi Kong ◽  
Abdul Hadi Mehmood ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endoplasmic Reticulum ◽  
Cell Death ◽  
Fluorescent Probe ◽  
Hypochlorous Acid ◽  
Living Cells ◽  
Oxygen Species ◽  
Cell Dysfunction ◽  
Two Photon ◽  
Endoplasmic Reticulum Targeting

As an important reactive oxygen species, hypochlorous acid (HClO) could irreversibly destroy Ca2+-ATPase to inhibit Ca2+ transport in the endoplasmic reticulum (ER), which may eventually lead to cell dysfunction and cell death.

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