A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay

2020 ◽  
Vol 12 (40) ◽  
pp. 4858-4866
Author(s):  
Takaaki Abe ◽  
Shunya Okamoto ◽  
Akinobu Taniguchi ◽  
Michiyasu Fukui ◽  
Akinobu Yamaguchi ◽  
...  
Keyword(s):  
Injection Molding ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Device Driver ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay

In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.

An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

scholarly journals Detection of Plasmodium Aldolase Using a Smartphone and Microfluidic Enzyme Linked Immunosorbent Assay

2017 ◽  
Vol 2017 ◽  
pp. 1-4
Author(s):  
Nikhil S. Gopal ◽  
Ruben Raychaudhuri
Keyword(s):  
Immunosorbent Assay ◽  
Microfluidic Chip ◽  
Rural Areas ◽  
Color Change ◽  
Low Cost ◽  
Sandwich Elisa ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cost System ◽  
Therapeutic Outcomes

Background. Malaria control efforts are limited in rural areas. A low-cost system to monitor response without the use of electricity is needed. Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. A three-part system using ELISA was developed consisting of a microfluidic chip, hand crank centrifuge, and a smartphone. Methods. A circular microfluidic chip was fabricated using clear acrylic and a CO2 laser. A series of passive valves released reagents at precise times based upon centrifugal force. Color change was measured via smartphone camera using an application programmed in Java. The microchip was compared to a standard 96-well sandwich ELISA. Results. Results from standard ELISA were compared to microchip at varying concentrations (1–10 ng/mL). Over 15 different microfluidic patterns were tested, and a final prototype of the chip was created. The prototype microchip was compared to standard sandwich ELISA (n=20) using samples of recombinant aldolase. Color readings of standard ELISA and microfluidic microchip showed similar results. Conclusion. A low-cost microfluidic system could detect and follow therapeutic outcomes in rural areas and identify resistant strains.

Micro OS-ELISA: Rapid noncompetitive detection of a small biomarkerpeptide by open-sandwich enzyme-linked immunosorbent assay (OS-ELISA) integrated into microfluidic device

Lab on a Chip ◽  
2010 ◽  
Vol 10 (1) ◽  
pp. 92-100 ◽  
Author(s):  
Masaki Ihara ◽  
Amane Yoshikawa ◽  
Yushu Wu ◽  
Hiroko Takahashi ◽  
Kazuma Mawatari ◽  
...  
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay

Direct digital manufacturing of a mini-centrifuge-driven centrifugal microfluidic device and demonstration of a smartphone-based colorimetric enzyme-linked immunosorbent assay

2016 ◽  
Vol 8 (2) ◽  
pp. 256-262 ◽  
Author(s):  
Yoshiaki Ukita ◽  
Yuichi Utsumi ◽  
Yuzuru Takamura
Keyword(s):  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Three Dimensional ◽  
Enzyme Linked Immunosorbent Assay ◽  
Digital Manufacturing ◽  
3D Printer ◽  
Direct Digital Manufacturing

This study reports the first demonstration of an enzyme-linked immunosorbent assay (ELISA) using a microfluidic device that was fabricated in a three-dimensional (3D) printer.

Surface Modification for Enhancing Antibody Binding on Polymer-Based Microfluidic Device for Enzyme-Linked Immunosorbent Assay

Langmuir ◽  
2006 ◽  
Vol 22 (22) ◽  
pp. 9458-9467 ◽  
Author(s):  
Yunling Bai ◽  
Chee Guan Koh ◽  
Megan Boreman ◽  
Yi-Je Juang ◽  
I-Ching Tang ◽  
...  
Keyword(s):  
Surface Modification ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay
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