An integrated microfluidic system for on-chip enrichment and quantification of circulating extracellular vesicles from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3305-3315 ◽  
Author(s):  
Yi-Sin Chen ◽  
Yu-Dong Ma ◽  
Chihchen Chen ◽  
Shu-Chu Shiesh ◽  
Gwo-Bin Lee
Keyword(s):  
Extracellular Vesicles ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Magnetic Beads ◽  
Extracellular Vesicle ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Whole Blood ◽  
On Chip ◽  
Chip Enrichment

An integrated microfluidic system was developed for extracellular vesicle (EV) enrichment and quantification by using anti-CD63-coated magnetic beads and an on-chip enzyme-linked immunosorbent assay in human whole blood.

A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay

2020 ◽  
Vol 12 (40) ◽  
pp. 4858-4866
Author(s):  
Takaaki Abe ◽  
Shunya Okamoto ◽  
Akinobu Taniguchi ◽  
Michiyasu Fukui ◽  
Akinobu Yamaguchi ◽  
...  
Keyword(s):  
Injection Molding ◽  
Microfluidic Device ◽  
Immunosorbent Assay ◽  
Device Driver ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay

In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding.

scholarly journals Enzyme-Linked Immunosorbent Assay for Detection of Plasmodium falciparum Histidine-Rich Protein 2 in Blood, Plasma, and Serum

2008 ◽  
Vol 15 (6) ◽  
pp. 1012-1018 ◽  
Author(s):  
Carolyne M. Kifude ◽  
Halli G. Rajasekariah ◽  
David J. Sullivan ◽  
V. Ann Stewart ◽  
Evelina Angov ◽  
...  
Keyword(s):  
Plasmodium Falciparum ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Linear Range ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Target Concentration ◽  
Intervention Trials ◽  
Lower Limits ◽  
Asexual Cycle

ABSTRACT Microscopy, the gold standard for the detection and quantification of malaria parasites in blood, is in many aspects deficient for this purpose. The method is poorly reproducible and can be inaccurate because Plasmodium falciparum parasites sequester for a portion of each asexual cycle. Due to these deficiencies, biomarkers such as P. falciparum histidine-rich protein 2 (PfHRP2) are increasingly being used. In this study, we evaluated the use of a commercial PfHRP2 enzyme-linked immunosorbent assay (ELISA) kit with some procedural modifications. We determined the linear range of the assay, including the lower limits of detection and quantitation, using recombinant PfHRP2 (rPfHRP2). In 10 repeat experiments, the linear range of optical densities (ODs) at 450 to 650 nm was from 0.05 ± 0.002 to 2.28 ± 0.042, corresponding to 3.91 to 250 ng/ml of rPfHRP2. The coefficient of variation (CV) at each target concentration ranged from 1.93 to 8.07%. Using cultured parasites, we confirmed the linear range of ODs as well as the association between the PfHRP2 ELISA results and the microscopic parasite densities. For whole-blood samples spiked with cultured, washed, ring-stage-infected red blood cells (iRBCs), the linear range was 11.7 to 750 iRBCs/μl, with CVs of 0.29 to 7.56%. The same spiked samples evaluated by microscopists had similar sensitivities, but the CVs were unacceptably high (20.7 to 161.6%). Stock rPfHRP2 was stable through four freeze-thaw cycles (P < 0.05; paired t test). When different patient sample types at different concentrations within the linear range of the assay are compared, the recoveries of PfHRP2 from blood and serum were within ±20%, whereas the recoveries from plasma ranged between +35 and −41%. We conclude that PfHRP2 ELISA using whole-blood and serum samples is a suitable adjunct to microscopy and could ultimately benefit malaria intervention trials.

scholarly journals Development of an automated on-chip bead-based ELISA platform

2015 ◽  
Vol 7 (19) ◽  
pp. 8472-8477 ◽  
Author(s):  
Jennifer Campbell ◽  
Nira R. Pollock ◽  
Andre Sharon ◽  
Alexis F. Sauer-Budge
Keyword(s):  
Immunosorbent Assay ◽  
Lab On A Chip ◽  
Enzyme Linked Immunosorbent Assay ◽  
Liquid Samples ◽  
On Chip

We present a lab-on-a-chip and associated instrument for heterogeneous enzyme-linked immunosorbent assay (ELISA)-based detection of proteins from liquid samples.

scholarly journals On-Chip Titration of an Anticoagulant Argatroban and Determination of the Clotting Time within Whole Blood or Plasma Using a Plug-Based Microfluidic System

2006 ◽  
Vol 78 (14) ◽  
pp. 4839-4849 ◽  
Author(s):  
Helen Song ◽  
Hung-Wing Li ◽  
Matthew S. Munson ◽  
Thuong G. Van Ha ◽  
Rustem F. Ismagilov
Keyword(s):  
Whole Blood ◽  
Microfluidic System ◽  
Clotting Time ◽  
On Chip

scholarly journals Detection of Plasmodium Aldolase Using a Smartphone and Microfluidic Enzyme Linked Immunosorbent Assay

2017 ◽  
Vol 2017 ◽  
pp. 1-4
Author(s):  
Nikhil S. Gopal ◽  
Ruben Raychaudhuri
Keyword(s):  
Immunosorbent Assay ◽  
Microfluidic Chip ◽  
Rural Areas ◽  
Color Change ◽  
Low Cost ◽  
Sandwich Elisa ◽  
Microfluidic System ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cost System ◽  
Therapeutic Outcomes

Background. Malaria control efforts are limited in rural areas. A low-cost system to monitor response without the use of electricity is needed. Plasmodium aldolase is a malaria biomarker measured using enzyme linked immunosorbent assay (ELISA) techniques. A three-part system using ELISA was developed consisting of a microfluidic chip, hand crank centrifuge, and a smartphone. Methods. A circular microfluidic chip was fabricated using clear acrylic and a CO2 laser. A series of passive valves released reagents at precise times based upon centrifugal force. Color change was measured via smartphone camera using an application programmed in Java. The microchip was compared to a standard 96-well sandwich ELISA. Results. Results from standard ELISA were compared to microchip at varying concentrations (1–10 ng/mL). Over 15 different microfluidic patterns were tested, and a final prototype of the chip was created. The prototype microchip was compared to standard sandwich ELISA (n=20) using samples of recombinant aldolase. Color readings of standard ELISA and microfluidic microchip showed similar results. Conclusion. A low-cost microfluidic system could detect and follow therapeutic outcomes in rural areas and identify resistant strains.

Enzyme-linked immunosorbent assay for determination of cyclosporin a in the whole blood

2011 ◽  
Vol 5 (1) ◽  
pp. 72-75
Author(s):  
I. P. Andreeva ◽  
N. T. Vorobyeva ◽  
L. I. Vinnitsky ◽  
S. S. Bogush ◽  
E. M. Gavrilova ◽  
...  
Keyword(s):  
Cyclosporin A ◽  
Immunosorbent Assay ◽  
Whole Blood ◽  
Enzyme Linked Immunosorbent Assay

scholarly journals On-Chip Purification of Tetracyclines Based on Copper Ions Interaction

Sensors ◽  
2021 ◽  
Vol 21 (21) ◽  
pp. 7236
Author(s):  
Lorenzo Lunelli ◽  
Martina Germanis ◽  
Lia Vanzetti ◽  
Roberta Tatti ◽  
Cristina Potrich ◽  
...  
Keyword(s):  
Magnetic Beads ◽  
Copper Ions ◽  
Raw Milk ◽  
Microfluidic System ◽  
Bacterial Diseases ◽  
Animal Growth ◽  
Pre Treatment ◽  
On Chip ◽  
Set Up

Antibiotics are widely used to both prevent and treat bacterial diseases as well as promote animal growth. This massive use leads to the presence of residual antibiotics in food with severe consequences for human health. Limitations and regulations on the tolerated amount of antibiotics in food have been introduced and analytical methods have been developed. The bioanalytical methods usually employed to detect antibiotic residues, however, are time-consuming, expensive and laboratory-based. Novel methods with improved rapidity, portability and cost that are easy-to-use and sustainable are therefore highly desirable. In the attempt to fulfill this need, a microfluidic system was set up herein for the purification and pre-concentration of tetracyclines from raw milk selected as the case-study. The system includes a polymeric microfluidic chip containing magnetic beads loaded with copper to exploit the preferential interaction of tetracycline with divalent ions. The microfluidic system was demonstrated to efficiently pre-concentrate tetracycline, oxytetracycline and chlortetracycline with similar performances and efficiently purify tetracycline from raw milk without any pre-treatment. The simplified method described in this paper could be easily integrated in a compact and portable device for the in-field detection of tetracyclines, with the economic advantage of preventing food wastes and guaranteeing food safety.

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